scholarly journals Complete Nucleotide Sequence of a Novel Plasmid Bearing the High-Level Tigecycline Resistance Gene tet(X4)

2019 ◽  
Vol 63 (11) ◽  
Author(s):  
Liang-Xing Fang ◽  
Chong Chen ◽  
Dong-Ling Yu ◽  
Ruan-Yang Sun ◽  
Chao-Yue Cui ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Hybrid Plasmid ◽  
Content Type ◽  
Clinically Significant ◽  
High Level ◽  
Plasmid Backbone

ABSTRACT We reported the complete nucleotide sequence of a tet(X4)-carrying plasmid, pSTB20-1T, from a tigecycline-resistant Escherichia coli isolate in China. Sequence analysis indicated that pSTB20-1T contains a hybrid plasmid backbone and a tet(X4)-containing multidrug resistance region, likely originated through recombination of multiple plasmids. tet(X4) was flanked by two ISCR2, which may be responsible for tet(X4) mobilization. The occurrence and transmission of this novel hybrid plasmid may exacerbate the spread of the clinically significant tet(X4) gene.

scholarly journals Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli

2010 ◽  
Vol 78 (5) ◽  
pp. 1931-1942 ◽  
Author(s):  
Timothy J. Johnson ◽  
Dianna Jordan ◽  
Subhashinie Kariyawasam ◽  
Adam L. Stell ◽  
Nathan P. Bell ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Sequence Analysis ◽  
Rat Model ◽  
Antimicrobial Agents ◽  
Neonatal Meningitis ◽  
E Coli ◽  
High Level ◽  
Plasmid Backbone

ABSTRACT ColV plasmids of extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis. These plasmids are found significantly more often in ExPEC, including ExPEC associated with human neonatal meningitis and avian colibacillosis, than in commensal E. coli. Here we describe pAPEC-O103-ColBM, a hybrid RepFIIA/FIB plasmid harboring components of the ColV pathogenicity island and a multidrug resistance (MDR)-encoding island. This plasmid is mobilizable and confers the ability to cause septicemia in chickens, the ability to cause bacteremia resulting in meningitis in the rat model of human disease, and the ability to resist the killing effects of multiple antimicrobial agents and human serum. The results of a sequence analysis of this and other ColV plasmids supported previous findings which indicated that these plasmid types arose from a RepFIIA/FIB plasmid backbone on multiple occasions. Comparisons of pAPEC-O103-ColBM with other sequenced ColV and ColBM plasmids indicated that there is a core repertoire of virulence genes that might contribute to the ability of some ExPEC strains to cause high-level bacteremia and meningitis in a rat model. Examination of a neonatal meningitis E. coli (NMEC) population revealed that approximately 58% of the isolates examined harbored ColV-type plasmids and that 26% of these plasmids had genetic contents similar to that of pAPEC-O103-ColBM. The linkage of the ability to confer MDR and the ability contribute to multiple forms of human and animal disease on a single plasmid presents further challenges for preventing and treating ExPEC infections.

scholarly journals Complete Nucleotide Sequence of an IncI2 Plasmid CoharboringblaCTX-M-55andmcr-1

2016 ◽  
Vol 60 (8) ◽  
pp. 5014-5017 ◽  
Author(s):  
Jian Sun ◽  
Xing-Ping Li ◽  
Run-Shi Yang ◽  
Liang-Xing Fang ◽  
Wei Huo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Sequence Similarity ◽  
Conjugative Plasmid ◽  
Insertion Sequences ◽  
Content Type ◽  
Extensive Sequence ◽  
Extended Spectrum ◽  
Lactamase Gene

ABSTRACTWe report the complete nucleotide sequence of a plasmid, pA31-12, carryingblaCTX-M-55andmcr-1from a chickenEscherichia coliisolate. pA31-12 has an IncI2 replicon that displays extensive sequence similarity with pHN1122-1-borneblaCTX-M-55and pHNSHP45-bornemcr-1. Insertion sequences ISEcp1and ISApl1are responsible for the mobilization ofblaCTX-M-55andmcr-1, respectively. The colocalization ofmcr-1with an extended-spectrum β-lactamase gene on a conjugative plasmid may accelerate the dissemination of both genes by coselection.

scholarly journals Complete Nucleotide Sequence of the IncN Plasmid Encoding IMP-6 and CTX-M-2 from Emerging Carbapenem-Resistant Enterobacteriaceae in Japan

2014 ◽  
Vol 59 (2) ◽  
pp. 1356-1359 ◽  
Author(s):  
Shizuo Kayama ◽  
Norifumi Shigemoto ◽  
Ryuichi Kuwahara ◽  
Kenshiro Oshima ◽  
Hideki Hirakawa ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Dna Sequence ◽  
Complete Nucleotide Sequence ◽  
Carbapenem Resistance ◽  
Content Type ◽  
Carbapenem Resistant ◽  
The Family ◽  
Carbapenem Resistant Enterobacteriaceae

ABSTRACTWe have determined the DNA sequence ofKlebsiella pneumoniaemultidrug resistance plasmid pKPI-6, which is a self-transmissible IncN-type plasmid. pKPI-6 harboringblaIMP-6andblaCTX-M-2confers a stealth-type carbapenem resistance phenotype on members of the familyEnterobacteriaceaethat is not detectable with imipenem. pKPI-6 is already epidemic in Japan, favoring the dissemination of IMP-6 and CTX-M-2 in members of the familyEnterobacteriaceae.

scholarly journals Nucleotide Sequence Analysis of the Enteropathogenic Escherichia coli Adherence Factor Plasmid pMAR7

2006 ◽  
Vol 74 (9) ◽  
pp. 5408-5413 ◽  
Author(s):  
Carl Brinkley ◽  
Valerie Burland ◽  
Rogéria Keller ◽  
Debra J. Rose ◽  
Adam T. Boutin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Horizontal Transfer ◽  
Complete Nucleotide Sequence ◽  
Nucleotide Sequence Analysis ◽  
Enteropathogenic Escherichia Coli ◽  
Type Iv ◽  
Genes Encoding ◽  
Virulence Regulator

ABSTRACT The complete nucleotide sequence was determined for pMAR7, an enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid that contains genes encoding a type IV attachment pilus (Bfp) and the global virulence regulator per. Prototypic EAF plasmid pMAR7 is self-transmissible, unlike the smaller EAF plasmid pB171, which has no genes encoding conjugative functions. The tra locus, a highly conserved 33-kb segment found in pMAR7, is similar to the tra (conjugation) region of the F plasmid. ISEc13 copies flanking the pMAR7 tra region could potentially mobilize or delete the tra genes. Hybridization of 134 EPEC strains showed that a complete tra region is present only in strains of the EPEC1 clonal group. This study confirms EPEC's potential for dissemination of virulence attributes by horizontal transfer of the EAF plasmid.

scholarly journals Complete Nucleotide Sequence ofcfr-Carrying IncX4 Plasmid pSD11 from Escherichia coli

2014 ◽  
Vol 59 (1) ◽  
pp. 738-741 ◽  
Author(s):  
Jian Sun ◽  
Hui Deng ◽  
Liang Li ◽  
Mu-Ya Chen ◽  
Liang-Xing Fang ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
The United States ◽  
Content Type

ABSTRACTWe report the complete nucleotide sequence of a plasmid carrying the multiresistance genecfr. This plasmid was isolated from anEscherichia colistrain of swine origin in 2011. This 37,672-bp plasmid, pSD11, had an IncX4 backbone similar to those of the IncX4 plasmids obtained from the United States and Australia, in which thecfrgene was flanked by two copies of IS26and a truncated Tn1331was inserted.

scholarly journals Complete Nucleotide Sequence of an Escherichia coli Sequence Type 410 Strain Carrying bla NDM-5 on an IncF Multidrug Resistance Plasmid and bla OXA-181 on an IncX3 Plasmid

2018 ◽  
Vol 6 (5) ◽  
Author(s):  
Søren Overballe-Petersen ◽  
Louise Roer ◽  
Kim Ng ◽  
Frank Hansen ◽  
Ulrik S. Justesen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Nucleotide Sequence ◽  
Resistance Genes ◽  
Complete Nucleotide Sequence ◽  
Sequence Type ◽  
Nanopore Sequencing ◽  
Incompatibility Group ◽  
E Coli ◽  
Resistance Plasmid

ABSTRACT Using Nanopore sequencing, we describe here the circular genome of an Escherichia coli sequence type 410 (ST410) strain with five closed plasmids. A large 111-kb incompatibility group F (IncF) plasmid harbored bla NDM-5 and 16 other resistance genes. A 51-kb IncX3 plasmid carried QnrS1 and bla OXA-181 . E. coli isolates with both bla NDM-5 and bla OXA-181 carbapenemases are rare.

scholarly journals Complete Sequence of ablaKPC-Harboring Cointegrate Plasmid Isolated from Escherichia coli

2015 ◽  
Vol 59 (5) ◽  
pp. 2956-2959 ◽  
Author(s):  
Kalyan D. Chavda ◽  
Liang Chen ◽  
Michael R. Jacobs ◽  
Albert D. Rojtman ◽  
Robert A. Bonomo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Horizontal Transfer ◽  
Complete Nucleotide Sequence ◽  
Complete Sequence ◽  
Plasmid Transfer ◽  
Content Type

ABSTRACTHorizontal transfer ofblaKPC-harboring plasmids contributes significantly to the inter- and intraspecies spread ofKlebsiella pneumoniaecarbapenemase (KPC). Here we report the complete nucleotide sequence of ablaKPC-harboring IncFIA plasmid, pBK32533, fromEscherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates howblaKPCcan be spread from a nonconjugative plasmid through cointegration.

scholarly journals Complete Nucleotide Sequence of a Conjugative Plasmid CarryingblaPER-1

2015 ◽  
Vol 59 (6) ◽  
pp. 3582-3584 ◽  
Author(s):  
Ruichao Li ◽  
Marcus Ho Yin Wong ◽  
Yuanjie Zhou ◽  
Edward Wai-chi Chan ◽  
Sheng Chen
Keyword(s):  
Multidrug Resistance ◽  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Vibrio Parahaemolyticus ◽  
Conjugative Plasmid ◽  
Complex Class ◽  
Class 1 Integron ◽  
Content Type ◽  
Transmissible Plasmid ◽  
Class 1

ABSTRACTThe nucleotide sequence of a self-transmissible plasmid pVPH1 harboringblaPER-1fromVibrio parahaemolyticuswas determined. pVPH1 was 183,730 bp in size and shared a backbone similar to pAQU1 and pAQU2, differing mainly in an ∼40-kb multidrug resistance (MDR) region. A complex class 1 integron was identified together with ISCR1andblaPER-1(ISCR1-blaPER-1-gst-abct-qacEΔ1-sul1), which was shown to form a circular intermediate playing an important role in the dissemination ofblaPER-1.

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