scholarly journals Association of Composite IS26-sul3Elements with Highly Transmissible IncI1 Plasmids in Extended-Spectrum-β-Lactamase-Producing Escherichia coli Clones from Humans

2011 ◽  
Vol 55 (5) ◽  
pp. 2451-2457 ◽  
Author(s):  
Tânia Curiao ◽  
Rafael Cantón ◽  
M. Pilar Garcillán-Barcia ◽  
Fernando de la Cruz ◽  
Fernando Baquero ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Comparative Analysis ◽  
Transposable Elements ◽  
In Silico ◽  
Insertion Element ◽  
Content Type ◽  
Class 1 Integrons ◽  
Extended Spectrum ◽  
Class 1 ◽  
M 14

ABSTRACTThe association of an IS440-sul3platform with Tn21class 1 integrons carried by IncI1 plasmids encoding extended-spectrum β-lactamases (ESBLs; mainly SHV-12 and CTX-M-14) among worldwideEscherichia coliclones of phylogroups A (ST10, ST23, and ST46), B1 (ST155, ST351, and ST359), and D/B2 (ST131) is reported. Anin silicocomparative analysis ofsul3elements available in the GenBank database shows the evolution ofsul3platforms by hosting different transposable elements facilitating the potential genesis of IS26composite transposons and further insertion element-mediated promoted arrangements.

Antimicrobial Resistance, Extended-Spectrum β-Lactamase Productivity, and Class 1 Integrons in Escherichia coli from Healthy Swine

2015 ◽  
Vol 78 (8) ◽  
pp. 1442-1450 ◽  
Author(s):  
KANJANA CHANGKAEW ◽  
APIRADEE INTARAPUK ◽  
FUANGFA UTRARACHKIJ ◽  
CHIE NAKAJIMA ◽  
ORASA SUTHIENKUL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Intestinal Flora ◽  
Class 1 Integron ◽  
Potential Health ◽  
Gene Cassettes ◽  
E Coli ◽  
Class 1 Integrons ◽  
Extended Spectrum ◽  
Class 1

Administration of antimicrobials to food-producing animals increases the risk of higher antimicrobial resistance in the normal intestinal flora of these animals. The present cross-sectional study was conducted to investigate antimicrobial susceptibility and extended-spectrum β-lactamase (ESBL)–producing strains and to characterize class 1 integrons in Escherichia coli in healthy swine in Thailand. All 122 of the tested isolates had drug-resistant phenotypes. High resistance was found to ampicillin (98.4% of isolates), chloramphenicol (95.9%), gentamicin (78.7%), streptomycin (77.9%), tetracycline (74.6%), and cefotaxime (72.1%). Fifty-four (44.3%) of the E. coli isolates were confirmed as ESBL-producing strains. Among them, blaCTX-M (45 isolates) and blaTEM (41 isolates) were detected. Of the blaCTX-M-positive E. coli isolates, 37 carried the blaCTX-M-1 cluster, 12 carried the blaCTX-M-9 cluster, and 5 carried both clusters. Sequence analysis revealed blaTEM-1, blaTEM-135, and blaTEM-175 in 38, 2, and 1 isolate, respectively. Eighty-seven (71%) of the 122isolates carried class 1 integrons, and eight distinct drug-resistance gene cassettes with seven different integron profiles were identified in 43 of these isolates. Gene cassettes were associated with resistance to aminoglycosides (aadA1, aadA2, aadA22, or aadA23), trimethoprim (dfrA5, dfrA12, or dfrA17), and lincosamide (linF). Genes encoding β-lactamases were not found in class 1 integrons. This study is the first to report ESBL-producing E. coli with a class 1 integron carrying the linF gene cassette in swine in Thailand. Our findings confirm that swine can be a reservoir of ESBL-producing E. coli harboring class 1 integrons, which may become a potential health risk if these integrons are transmitted to humans. Intensive analyses of animal, human, and environmental isolates are needed to control the spread of ESBL-producing E. coli strains.

scholarly journals Molecular Identification of Extended-Spectrum-β-Lactamase Genes from Enterobacteriaceae Isolated from Healthy Human Carriers in Switzerland

2011 ◽  
Vol 56 (3) ◽  
pp. 1609-1612 ◽  
Author(s):  
Nadine Geser ◽  
Roger Stephan ◽  
Bozena M. Korczak ◽  
Lothar Beutin ◽  
Herbert Hächler
Keyword(s):  
Escherichia Coli ◽  
Pcr Analysis ◽  
Healthy Human ◽  
Fecal Samples ◽  
Content Type ◽  
Healthy Humans ◽  
Extended Spectrum ◽  
High Degree ◽  
Esbl Producers ◽  
M 14

ABSTRACTIn this study, fecal samples from 586 healthy humans were investigated to determine the occurrence of extended-spectrum-β-lactamase (ESBL)-producingEnterobacteriaceaein Swiss people. A total of 5.8% of the human fecal samples yielded ESBL producers, and all of the 34 isolated strains wereEscherichia coli. PCR analysis revealed that 14 strains produced CTX-M-15, 10 produced CTX-M-1, 7 strains produced CTX-M-14, and 2 strains produced CTX-M-2 ESBLs. One strain produced SHV-12 ESBL. Of the 34 isolates, 15 produced additional TEM-1 broad-spectrum β-lactamases. By serotyping, a high degree of diversity among the strains was found.

scholarly journals Relevance of class 1 integrons and extended-spectrum β-lactamases in drug-resistant Escherichia coli

2013 ◽  
Vol 8 (4) ◽  
pp. 1251-1255 ◽  
Author(s):  
LI-TAO LIU ◽  
LI-HONG WAN ◽  
XIAO-HONG SONG ◽  
YAO XIONG ◽  
SHAO-JU JIN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistant ◽  
Class 1 Integrons ◽  
Extended Spectrum ◽  
Class 1

scholarly journals High Rate of Fecal Carriage of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Healthy Children in Gipuzkoa, Northern Spain

2014 ◽  
Vol 58 (3) ◽  
pp. 1822-1824 ◽  
Author(s):  
María Fernández-Reyes ◽  
Diego Vicente ◽  
María Gomariz ◽  
Olatz Esnal ◽  
Joseba Landa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Rate ◽  
Healthy Children ◽  
Northern Spain ◽  
Content Type ◽  
Fecal Carriage ◽  
Extended Spectrum ◽  
High Prevalence ◽  
Stool Samples ◽  
M 14

ABSTRACTThe prevalence of extended-spectrum-β-lactamase-producingEnterobacteriaceae(ESBLPE) was studied in stool samples from 125 8- to 16-month-old healthy children. Twenty-four percent of them and 10.7% of the 318 fecal samples studied yielded extended-spectrum-β-lactamase-producingEscherichia coli, with the types being SHV-12, CTX-M-1, CTX-M-14, and TEM-52, the most common types of β-lactamases. This high prevalence of ESBLPE in healthy people, which is to our knowledge the highest currently reported in Europe, may represent a risk for increased infections by these organisms in the future.

scholarly journals Widespread Dissemination of CTX-M-15 Genotype Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae among Patients Presenting to Community Hospitals in the Southeastern United States

2013 ◽  
Vol 58 (2) ◽  
pp. 1200-1202 ◽  
Author(s):  
Luke F. Chen ◽  
Joshua T. Freeman ◽  
Brad Nicholson ◽  
Anna Keiger ◽  
Sarah Lancaster ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
North Carolina ◽  
Southeastern United States ◽  
Sequence Type ◽  
Community Hospitals ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Widespread Dissemination ◽  
M 14

ABSTRACTExtended-spectrum-β-lactamase (ESBL)-producing organisms are increasingly prevalent. We determined the characteristics of 66 consecutive ESBL-producing isolates from six community hospitals in North Carolina and Virginia from 2010 to 2012. Fifty-three (80%) ESBL-producing isolates contained CTX-M enzymes; CTX-M-15 was found in 68% ofEscherichia coliand 73% ofKlebsiellaisolates. Sequence type 131 (ST131) was the commonest type ofE. coli, accounting for 48% of CTX-M-15-producing and 66% of CTX-M-14-producing isolates. In conclusion, the CTX-M genotype and ST131E. coliwere common among ESBL isolates from U.S. community hospitals.

scholarly journals Occurrence of extended-spectrum beta-lactamases in human and bovine isolates of Escherichia coli from Oyo state, Nigeria

2014 ◽  
Vol 8 (06) ◽  
pp. 774-779 ◽  
Author(s):  
Christiana Inwezerua ◽  
Nuno Mendonça ◽  
Vera Calhau ◽  
Sara Domingues ◽  
Olufemi Ezekiel Adeleke ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recipient Strain ◽  
Beta Lactamases ◽  
E Coli ◽  
Class 1 Integrons ◽  
Extended Spectrum ◽  
Plasmid Replicon ◽  
Class 1 ◽  
Extended Spectrum Beta Lactamases

Introduction: The main objective of the study was the molecular characterization of extended spectrum β-lactamases (ESBL) in Escherichia coli isolates collected from human and bovine samples in Oyo state, Nigeria. Methodology: Between August 2010-2011, 114 E. coli isolates were collected from hospitals (n = 57) and bovine (n = 57). PCR and sequencing were used for identification of ESBLs, upstream sequences, plasmid-mediated quinolone resistance (PMQR) genes and class 1 integrons. Plasmid incompatibility groups were identified among ESBL-positive isolates by PCR. Genetic relatdness was assessed by rep-PCR and MLST. Transfer of ESBL determinants to the recipient strain E. coli J53 was performed by broth mating assays. Results: CTX-M15 was the unique ESBL found in eight human isolates. Six CTX-M-15 producers also carry the aac(6’)-lb-cr gene and/or qnrB gene, and class 1 integrons. FIA, FIB, H11, H12, F, Y and K were the plasmid replicon types found. CTX-M-15 and PMQR determinants were transferred by conjugation in two E. coli assigned by MLST to ST131 and ST2695, a new allele. Conclusions: The study highlights the dissemination hability of CTX-M-15 associated with PMQR, and the presence of class 1 integrons, able to capture additional genes, justifying the urgent need of antimicrobial resistance surveillance in Nigeria.

scholarly journals Dissemination of pCT-Like IncK Plasmids Harboring CTX-M-14 Extended-Spectrum β-Lactamase among Clinical Escherichia coli Isolates in the United Kingdom

2012 ◽  
Vol 56 (6) ◽  
pp. 3376-3377 ◽  
Author(s):  
Hiran Dhanji ◽  
Parmina Khan ◽  
Jennifer L. Cottell ◽  
Laura J. V. Piddock ◽  
Jiancheng Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quality Assurance ◽  
United Kingdom ◽  
Clinical Isolates ◽  
Content Type ◽  
E Coli ◽  
The United Kingdom ◽  
Extended Spectrum ◽  
Cattle Strain ◽  
M 14

ABSTRACTIncK plasmids encoding CTX-M-14 extended-spectrum β-lactamase (ESBL) and highly related to plasmid pCT were detected in 13 of 67 (19%) human clinical isolates ofEscherichia coliwith a group 9 CTX-M-type ESBL from the United Kingdom and in 2 quality assurance isolates. None of theseE. colistrains was related to the cattle strain from which pCT was originally characterized.

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