scholarly journals Characterization of a Novel Putative Xer-Dependent Integrative Mobile Element Carrying theblaNMC-ACarbapenemase Gene, Inserted into the Chromosome of Members of the Enterobacter cloacae Complex

2015 ◽  
Vol 59 (10) ◽  
pp. 6620-6624 ◽  
Author(s):  
Alberto Antonelli ◽  
Marco Maria D'Andrea ◽  
Vincenzo Di Pilato ◽  
Bruno Viaggi ◽  
Francesca Torricelli ◽  
...  
Keyword(s):  
Mobile Element ◽  
Enterobacter Cloacae ◽  
Bioinformatic Analysis ◽  
Pcr Analysis ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene ◽  
Enterobacter Ludwigii ◽  
Enterobacter Asburiae

ABSTRACTAnEnterobacter ludwigiistrain was isolated during routine screening of a Japanese patient for carriage of carbapenem-resistantEnterobacteriaceae. PCR analysis revealed theblaNMC-Acarbapenemase gene. Whole-genome sequencing revealed thatblaNMC-Awas inserted in the chromosome and associated with a novel 29.1-kb putative Xer-dependent integrative mobile element, named EludIMEX-1. Bioinformatic analysis identified similar elements in the genomes of anEnterobacter asburiaestrain and of otherEnterobacter cloacaecomplex strains, confirming the mobile nature of this element.

scholarly journals Enterobacter cloacae Complex Isolates Harboring blaNMC-A or blaIMI-Type Class A Carbapenemase Genes on Novel Chromosomal Integrative Elements and Plasmids

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
David A. Boyd ◽  
Laura F. Mataseje ◽  
Ross Davidson ◽  
Johannes A. Delport ◽  
Jeff Fuller ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Reference Laboratory ◽  
Chromosomal Locus ◽  
Complex Species ◽  
Content Type ◽  
Class A ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene ◽  
Enterobacter Ludwigii ◽  
Enterobacter Cloacae Complex

ABSTRACT Carbapenem-resistant Enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by PCR for seven common carbapenemase gene groups, namely, KPC, NDM, OXA-48, VIM, IMP, GES, and NMC-A/IMI. Nineteen of the submitted isolates (1.7%) were found to harbor Ambler class A bla NMC-A or bla IMI-type carbapenemases. All 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprofloxacin. Most isolates (17/19) gave positive results with the Carba-NP test for phenotypic carbapenemase detection. Isolates were genetically diverse by pulsed-field gel electrophoresis macrorestriction analysis, multilocus sequence typing, and hsp60 gene analysis. The genes were found in various Enterobacter cloacae complex species; however, bla NMC-A was highly associated with Enterobacter ludwigii. Whole-genome sequencing and bioinformatics analysis revealed that all NMC-A (n = 10), IMI-1 (n = 5), and IMI-9 (n = 2) producers harbored the carbapenemase gene on EludIMEX-1-like integrative mobile elements (EcloIMEXs) located in the identical chromosomal locus. Two novel genes, bla IMI-5 and bla IMI-6, were harbored on different IncFII-type plasmids. Enterobacter cloacae complex isolates harboring bla NMC-A/IMI-type carbapenemases are relatively rare in Canada. Though mostly found integrated into the chromosome, some variants are located on plasmids that may enhance their mobility potential.

scholarly journals Characterization of an Enterobacter cloacae Strain Producing both KPC and NDM Carbapenemases by Whole-Genome Sequencing

2015 ◽  
Vol 59 (10) ◽  
pp. 6625-6628 ◽  
Author(s):  
Wenjing Wu ◽  
Yu Feng ◽  
Alessandra Carattoli ◽  
Zhiyong Zong
Keyword(s):  
Whole Genome Sequencing ◽  
Bloodstream Infection ◽  
Genome Sequencing ◽  
Carbapenem Resistance ◽  
Enterobacter Cloacae ◽  
Whole Genome ◽  
Content Type ◽  
Carbapenem Resistant

ABSTRACTA carbapenem-resistantEnterobacter cloacaestrain, WCHECl-14653, causing a fatal bloodstream infection, was characterized by genome sequencing and conjugation experiments. The strain carried two carbapenemase genes,blaNDM-1andblaKPC-2, on separate IncF plasmids. The coexistence ofblaNDM-1andblaKPC-2conferred slightly higher-level carbapenem resistance compared with that ofblaNDM-1orblaKPC-2alone, and the coexistence of two IncF plasmids may generate new platforms for spreading carbapenemase genes.

scholarly journals Characterization of Multiple NDM-1-Producing Enterobacteriaceae Isolates from the Same Patient

2015 ◽  
Vol 59 (6) ◽  
pp. 3648-3651 ◽  
Author(s):  
Nathalie Tijet ◽  
David Richardson ◽  
Gregory MacMullin ◽  
Samir N. Patel ◽  
Roberto G. Melano
Keyword(s):  
Escherichia Coli ◽  
Male Patient ◽  
Community Hospital ◽  
Enterobacter Cloacae ◽  
Blood Cultures ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Plasmid Analysis ◽  
Providencia Stuartii

ABSTRACTA male patient was admitted to a community hospital in Ontario, Canada, with an infected sacral ulcer after returning from India, where he was hospitalized. Carbapenem-resistantEscherichia coli(isolated from blood cultures),Enterobacter cloacae, andProvidencia stuartii(from urine samples), all positive forblaNDM-1, were recovered. Comparative NDM-1 plasmid analysis suggests both lateral plasmid transfer and independent acquisition of theblaNDM-1gene in these clinical isolates.

scholarly journals Nosocomial Outbreak of Extensively Drug-Resistant Acinetobacter baumannii Isolates Containing blaOXA-237 Carried on a Plasmid

2017 ◽  
Vol 61 (11) ◽  
Author(s):  
Andrea M. Hujer ◽  
Paul G. Higgins ◽  
Susan D. Rudin ◽  
Genevieve L. Buser ◽  
Steven H. Marshall ◽  
...  
Keyword(s):  
United States ◽  
Acinetobacter Baumannii ◽  
Health Care Facilities ◽  
Pcr Analysis ◽  
Drug Resistant ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Extensively Drug Resistant ◽  
Separate Branch ◽  
Carbapenemase Gene

ABSTRACT Carbapenem antibiotics are among the mainstays for treating infections caused by Acinetobacter baumannii, especially in the Northwest United States, where carbapenem-resistant A. baumannii remains relatively rare. However, between June 2012 and October 2014, an outbreak of carbapenem-resistant A. baumannii occurred in 16 patients from five health care facilities in the state of Oregon. All isolates were defined as extensively drug resistant. Multilocus sequence typing revealed that the isolates belonged to sequence type 2 (international clone 2 [IC2]) and were >95% similar as determined by repetitive-sequence-based PCR analysis. Multiplex PCR revealed the presence of a bla OXA carbapenemase gene, later identified as bla OXA-237. Whole-genome sequencing of all isolates revealed a well-supported separate branch within a global A. baumannii phylogeny. Pacific Biosciences (PacBio) SMRT sequencing was also performed on one isolate to gain insight into the genetic location of the carbapenem resistance gene. We discovered that bla OXA-237, flanked on either side by ISAba1 elements in opposite orientations, was carried on a 15,198-bp plasmid designated pORAB01-3 and was present in all 16 isolates. The plasmid also contained genes encoding a TonB-dependent receptor, septicolysin, a type IV secretory pathway (VirD4 component, TraG/TraD family) ATPase, an integrase, a RepB family plasmid DNA replication initiator protein, an alpha/beta hydrolase, and a BrnT/BrnA type II toxin-antitoxin system. This is the first reported outbreak in the northwestern United States associated with this carbapenemase. Particularly worrisome is that bla OXA-237 was carried on a plasmid and found in the most prominent worldwide clonal group IC2, potentially giving pORAB01-3 great capacity for future widespread dissemination.

scholarly journals Characterization of resistance mechanisms of Enterobacter cloacae Complex co-resistant to carbapenem and colistin

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shixing Liu ◽  
Renchi Fang ◽  
Ying Zhang ◽  
Lijiang Chen ◽  
Na Huang ◽  
...  
Keyword(s):  
Lipid A ◽  
Efflux Pump ◽  
Resistance Mechanism ◽  
Carbapenem Resistance ◽  
Enterobacter Cloacae ◽  
Resistance Mechanisms ◽  
Colistin Resistance ◽  
Carbapenem Resistant ◽  
Horizontal Spread

Abstract Background The emergence of carbapenem-resistant and colistin-resistant ECC pose a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in Enterobacter cloacae Complex (ECC) strains. Results This study showed that the mechanisms of carbapenem resistance in this study are: 1. Generating carbapenemase (7 of 19); 2. The production of AmpC or ESBLs combined with decreased expression of out membrane protein (12 of 19). hsp60 sequence analysis suggested 10 of 19 the strains belong to colistin hetero-resistant clusters and the mechanism of colistin resistance is increasing expression of acrA in the efflux pump AcrAB-TolC alone (18 of 19) or accompanied by a decrease of affinity between colistin and outer membrane caused by the modification of lipid A (14 of 19). Moreover, an ECC strain co-harboring plasmid-mediated mcr-4.3 and blaNDM-1 has been found. Conclusions This study suggested that there is no overlap between the resistance mechanism of co-resistant ECC strains to carbapenem and colistin. However, the emergence of strain co-harboring plasmid-mediated resistance genes indicated that ECC is a potential carrier for the horizontal spread of carbapenems and colistin resistance.

Characterization of the genetic environment of blaKPC in Escherichia coli isolates from hospitals in China

2020 ◽  
Vol 367 (11) ◽  
Author(s):  
Xuejing Yang ◽  
Yan Qi ◽  
Guoping Li ◽  
Yuying Wang ◽  
Zhengqing Lou ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Carbapenem Resistance ◽  
Pcr Analysis ◽  
Genetic Environment ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Infection Treatment ◽  
Hospital Infection Control ◽  
Klebsiella Pneumoniae Carbapenemases

ABSTRACT Carbapenem resistance in Enterobacteriaceae members has become a major challenge, and the genetic environment of blaKPC, encoding Klebsiella pneumoniae carbapenemases, has not been fully clarified in China. In this study, we aimed to explore the genetic environment of blaKPC in 25 carbapenem-resistant E. coli isolates from hospitals in Hangzhou Province, China. Antimicrobial susceptibility against 22 common antimicrobial agents was tested. Polymerase chain reaction (PCR) analysis was performed for screening of the resistent genes, such as blaKPC, blaCTX-M, blaTEM, blaSHV, blaNDM, qnrA, qnrB, qnrS, aac(6’)-Ib, armA and rmtB. The genetic environment of blaKPC were determinedin one isolate. blaKPC was detected by PCR in all the clinical E. coli isolates. There were no strains carrying blaNDM, qnrA and armA. The genetic environment of blaKPC showed that blaKPC dissemination is plasmid mediated and that it is located in the Tn3–Tn4401 transposon complex. Encoding of blaKPC-2 was responsible for carbapenem resistance in the 25 E. coli isolates. The genetic environment of blaKPC was characterized by the Tn3–Tn4401 complex. Our findings may provide a theoretical basis for clinical drug-resistance monitoring, anti-infection treatment and hospital infection control.

scholarly journals Molecular Characterization of Carbapenem-Resistant Enterobacter cloacae in 11 Chinese Cities

2018 ◽  
Vol 9 ◽  
Author(s):  
Chunmei Jin ◽  
Jiangang Zhang ◽  
Qi Wang ◽  
Hongbin Chen ◽  
Xiaojuan Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterobacter Cloacae ◽  
Chinese Cities ◽  
Carbapenem Resistant

scholarly journals Characterization of the Enterobacter Phage vB_EclM_CIP9

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Klara Wang ◽  
Marielou G. Tamayo ◽  
Tiffany V. Penner ◽  
Bradley W. M. Cook ◽  
Deborah A. Court ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Opportunistic Pathogen ◽  
Multidrug Resistant ◽  
Open Reading Frames ◽  
Resistant Isolate ◽  
Content Type ◽  
Hospital Acquired Infections ◽  
Hospital Acquired ◽  
Reading Frames

Enterobacter cloacae is an opportunistic pathogen that causes hospital-acquired infections in immunocompromised patients. Here, we describe vB_EclM_CIP9, a novel Enterobacter phage that infects a multidrug-resistant isolate of E. cloacae. Phage vB_EclM_CIP9 is a myovirus that has a 174,924-bp genome, with 296 predicted open reading frames.

scholarly journals New Variant of mcr-3 in an Extensively Drug-Resistant Escherichia coli Clinical Isolate Carrying mcr-1 and blaNDM-5

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Lu Liu ◽  
Yu Feng ◽  
Xiaoxia Zhang ◽  
Alan McNally ◽  
Zhiyong Zong
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Amino Acid Substitutions ◽  
Drug Resistant ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Resistant Genes ◽  
Extensively Drug Resistant ◽  
New Variant ◽  
Carbapenemase Gene

ABSTRACT A colistin- and carbapenem-resistant Escherichia coli clinical isolate was found to carry two plasmid-borne colistin-resistant genes, mcr-1 and the newly identified mcr-3, and a carbapenemase gene, bla NDM-5. mcr-3 is a new variant (mcr-3.5) in the isolate and encodes three amino acid substitutions compared with the original MCR-3. mcr-3 was carried by a TnAs3-like transposon on a self-transmissible IncP plasmid in the isolate, highlighting that mcr-3 may have widely spread.

scholarly journals Epidemiology of Carbapenem-Resistant Enterobacteriaceae Infections: Report from the China CRE Network

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
Yawei Zhang ◽  
Qi Wang ◽  
Yuyao Yin ◽  
Hongbin Chen ◽  
Longyang Jin ◽  
...  
Keyword(s):  
Polymyxin B ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene ◽  
Tertiary Hospitals ◽  
Enterobacteriaceae Infections ◽  
Intra Abdominal Infection ◽  
Tract Infections ◽  
Susceptibility Rate

ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) infection is highly endemic in China, but estimates of the infection burden are lacking. We established the incidence of CRE infection from a multicenter study that covered 25 tertiary hospitals in 14 provinces. CRE cases defined as carbapenem-nonsusceptible Citrobacter freundii, Escherichia coli, Enterobacter cloacae, or Klebsiella pneumoniae infections during January to December 2015 were collected and reviewed from medical records. Antimicrobial susceptibility testing and carbapenemase gene identification were performed. Among 664 CRE cases, most were caused by K. pneumoniae (73.9%), followed by E. coli (16.6%) and E. cloacae (7.1%). The overall CRE infection incidence per 10,000 discharges was 4.0 and differed significantly by region, with the highest in Jiangsu (14.97) and the lowest in Qinghai (0.34). Underlying comorbidities were found in 83.8% of patients; the median patient age was 62 years (range, 45 to 74 years), and 450 (67.8%) patients were male. Lower respiratory tract infections (65.4%) were the most common, followed by urinary tract infection (16.6%), intra-abdominal infection (7.7%), and bacteremia (7.7%). The overall hospital mortality rate was 33.5%. All isolates showed nonsusceptibility to carbapenems and cephalosporins. The susceptibility rate of polymyxin B was >90%. Tigecycline demonstrated a higher susceptibility rate against E. coli than against K. pneumoniae (90.9% versus 40.2%). Of 155 clinical isolates analyzed, 89% produced carbapenemases, with a majority of isolates producing KPC (50%) or NDM (33.5%)-type beta-lactamases among K. pneumoniae and E. coli. The incidence of CRE infection in China was 4.0 per 10,000 discharges. The patient-based disease burden in tertiary hospitals in China is severe, suggesting an urgent need to enhance infection control.

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