scholarly journals Penetration of cefixime into fibrin clots and in vivo efficacy against Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus.

1986 ◽  
Vol 30 (6) ◽  
pp. 913-916 ◽  
Author(s):  
M G Bergeron ◽  
A Turcotte
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
In Vivo Efficacy ◽  
And Staphylococcus Aureus ◽  
Fibrin Clots

scholarly journals Control of bacterial pathogens isolated from water using Actinomycetes extracts at Egerton University, Kenya

Bionatura ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Paul Njenga Waithaka ◽  
Eliud Mugu Gathuru ◽  
Benson Muriuki Githaiga ◽  
Jackline Njeri Kamunyi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Bacterial Pathogens ◽  
Diffusion Method ◽  
Significant Difference ◽  
Shallow Wells ◽  
And Staphylococcus Aureus

Diseases are the worst enemy to man currently. This study was aimed at isolating pathogenic bacteria from water obtained from shallow wells in Dundori Kenya. Also, the study aimed at testing the isolates for sensitivity to antibiotic metabolites previously extracted from Actinomycetes isolates from soils of Egerton University. Water samples were collected from shallow wells randomly selected from Dundori and abbreviated as A, B, C, D, and E. Bacterial pathogens were isolated from the water samples using the membrane filtration technique. The isolates were characterized using biochemical means. Antimicrobial sensitivity testing was carried out using Kirby Bauer disk diffusion method. Data analysis was carried out using the Statistical Package for Social Sciences (SPSS). Comparison of means was carried out using one way ANOVA. Shallow wells B, D and E were highly contaminated with pathogenic bacteria. Biochemical characterization of the isolates indicated that the most common isolates were Vibrio cholera, Klebsiella pneumoniae, Proteus sp, Escherichia coli, and Staphylococcus aureus. There was no significant difference between the zones of inhibition produced by the antibiotic metabolites (F=2.149 P=1340) when tested against the test isolates. There were no significant differences between the MIC's of the antibiotic metabolites on the bacterial pathogens (F=2.01 P=0.15). Water from some shallow wells in Dundori is highly contaminated with Klebsiella pneumoniae, Escherichia coli, Proteus sp., Vibrio cholerae and Staphylococcus aureus. The pathogens can effectively be controlled using antibiotics from the Actinomycetes. There is a need to sensitize the residents of the study area on ways of preventing seepage of contaminants into the shallow wells.

scholarly journals AÇÃO ANTIMICROBIANA DAS FLORES DE ANACARDIUM OCCIDENTALE E DO ÁCIDO ELÁGICO PRESENTE NO EXTRATO

2021 ◽  
Author(s):  
Pâmela Gomes Santos ◽  
Rosane Nassar Meireles Guerra ◽  
Josivan Regis Farias ◽  
Simone Batista Muniz ◽  
Danielle Cristine Gomes Franco
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Tenebrio Molitor ◽  
Anacardium Occidentale

Introdução: As infecções bacterianas têm aumentado significativamente nas últimas décadas, sobretudo aquelas ocasionadas por microrganismos multirresistentes. Assim, o uso de produtos naturais com finalidades terapêuticas surge com alvo de bioprospecção na busca de novos compostos com ação antimicrobiana. Além disso, o uso de insetos, como o Tenebrio molitor como modelo experimental para avaliação in vivo tem sido muito frequente, pois exige menos material em relação aos testes com animais vertebrados. Objetivo: O presente trabalho investigou o efeito citotóxico e ação antimicrobiana do extrato hidroalcoólico das flores de Anacardium occidentale (EHAo) e do ácido elágico. Material e Métodos: Avaliamos a citoxicidade de ácido elágico e do EHAo nas concentrações (1; 5 e 50mg/kg) em Tenebrio molitor. A ação antimicrobiana para Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa e Klebsiella pneumoniae e a toxicidade do ácido elágico, foi avaliada por microdiluição, segundo a norma M7-A6 do manual da Clinical and Laboratory Standards Institute – CLSI. Foi determinada a Concentração Bactericida Mínima (CBM) e concentração inibitória mínima (CIM), em culturas de 24 horas, incubadas à 37ºC. Resultados: No ensaio de citotoxidade aguda se verificou que nenhuma das concentrações usados foram tóxicas, pois não ocorreram óbitos e nem nenhuma anormalia morfológica nas larvas de Tenebrio molitor. Os testes de concentração inibitória mínima (CIM) de concentração bactericida mínima (CBM) mostraram que o EHAo apresentou ação bactericida para Enterococcus faecalis em todas as concentrações testadas. Para Staphylococcus aureus os resultados mostraram ação bactericida para as maiores concentrações e bacteriostática para a menor diluição. O ácido elágico teve ação bactericida apenas para Enterococcus faecalis. Para as bactérias Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa e Klebsiella pneumoniae as concentrações de EHAo e ácido elágico testadas não foram inibitórias. Conclusões: Os resultados mostraram baixa toxicidade tanto para o EHAo como para o ácido elágico e ainda, que o extrato apresentou melhor efeito antimicrobiano do que o ácido elágico, para Enterococcus faecalis e Staphylococcus aureus.

In vitro and in vivo efficacy of methyl oleate and palmitic acid against ESBL producing MDR Escherichia coli and Klebsiella pneumoniae

2020 ◽  
Vol 148 ◽  
pp. 104446
Author(s):  
Nagarajan Padmini ◽  
Nagasundaram Rashiya ◽  
Natesan Sivakumar ◽  
Narayanan Dhiraviam Kannan ◽  
Ramamoorthy Manjuladevi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Methyl Oleate ◽  
Klebsiella Pneumoniae ◽  
Palmitic Acid ◽  
In Vivo Efficacy

scholarly journals In vitro synergistic potentials of novel antibacterial combination therapies against Salmonella Typhimurium, Escherichia coli and Staphylococcus aureus

2019 ◽  
Author(s):  
Md Akil Hossain ◽  
Hae-Chul Park ◽  
Kwang-jick Lee ◽  
Sung-Won Park ◽  
Seung-Chun Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Virulence Factors ◽  
In Vitro Cytotoxicity ◽  
E Coli ◽  
And Staphylococcus Aureus

Abstract Background: Bacteria have remarkable abilities to acquire resistance against antibiotics by several mechanisms. New strategies are needed to block the development of resistance and to prolong the life of traditional antibiotics. This study aimed to increase the efficacy of existing antibiotics by combining them with the opportunistic phenolic compound gallic acid (GA) and its derivatives. Fractional inhibitory concentration (FIC) indexes of phenolic compound-antibiotic combinations against Salmonella enterica serovar Typhimurium, Escherichia coli and Staphylococcus aureus were determined. Based on the FIC indexes and clinical importance, 3 combinations were selected to evaluate their effects on the virulence factors of these bacteria. The in vitro cytotoxicity of GA and hamamelitannin in the Rattus norvegicus (IEC-6) cell line were evaluated. Results: Phenolic compounds demonstrated considerable antibacterial effects as the minimum inhibitory concentrations (MICs) of epigallocatechin, GA and hamamelitannin found against different strains were (32–1024), (128–1024) and (512–≥2048) μg/mL, respectively. The FIC indexes of the combined antibacterials against these strains were 0.281–1.016. The ultrastructural morphology and time-kill assays showed that the GA-ceftiofur combination, and hamamelitannin-erythromycin and GA-ampicillin combinations more efficiently inhibited the growth of S. Typhimurium and E. coli, respectively, compared to the individual antibiotics. Biofilm viability and the swimming and swarming motilities of S. Typhimurium in the presence of GA-ceftiofur and E. coli in the presence of the hamamelitannin-erythromycin and GA-ampicillin combinations were more competently inhibited than individual antimicrobials. The 50% inhibitory concentrations (IC50) of GA and hamamelitannin in IEC-6 cells were 564.55 μM and 988.54 μM, respectively. Conclusions: The phenolic compounds increase the efficacy of existing antibiotics might be by disrupting virulence factors. We can conclude that these antibacterial combinations are safe and can be potential medications to treat S. Typhimurium, E. coli and S. aureus infections in animals and humans. Further study to confirm this effect in in vivo system and to determine the precise mechanism of action should be undertaken to establish these combinations as medications.

scholarly journals In Vitro Activity of Rifabutin and Rifampin against Antibiotic-Resistant Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae

mSphere ◽  
2021 ◽  
Author(s):  
Bosul Lee ◽  
Jun Yan ◽  
Amber Ulhaq ◽  
Sarah Miller ◽  
Wonjae Seo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
General Effect ◽  
In Vitro Activity ◽  
Antibiotic Resistant ◽  
Content Type

Rifabutin has been recently described as a potential adjunctive therapy for antibiotic-resistant A. baumannii infections due to hypersensitivity in iron-depleted media, which may more closely mimic an in vivo environment. Here, we report that this hyperactivity is specific for A. baumannii , rather than being a general effect for other pathogens.

scholarly journals HPTLC AND GC-MS PROFILE OF BARLERIA LUPULINA LINDL. EXTRACTS AND THEIR EFFECT ON ENTERIC BACTERIAL PATHOGENS

2016 ◽  
Vol 8 ◽  
pp. 61
Author(s):  
Reshma Kumari ◽  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Bacterial Pathogens ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Water Soluble ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
And Staphylococcus Aureus

The antibacterial activity of acetone, methanol, and water-soluble extracts of both leaf and stem of Barleria lupulina were evaluated against some human pathogens viz., Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus. The highest antibacterial activities were exhibited at 100% concentrationof all the extracts against the bacterial pathogens. Acetone-soluble leaf (AL) and acetone-soluble stem (AS) extracts caused the maximum zone of inhibition against P. aeruginosa, and methanol-soluble leaf (ML) and methanol-soluble stem (MS) extracts against of Salmonella typhi. However, Klebsiella pneumoniae was inhibited by all the extracts.

Antimicrobial Activity of 2,5-Dihydroxy-3-methyl-1,4-benzoquinone from Embelia schimperi

2008 ◽  
Vol 63 (1-2) ◽  
pp. 47-50 ◽  
Author(s):  
Ooko Selline Awino ◽  
Paul Chepkwony Kiprono ◽  
Kipkemboi Pius Keronei ◽  
Festus Kaberia ◽  
Andrew Ambogo Obala
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Klebsiella Pneumoniae ◽  
Crude Extract ◽  
Pure Compound ◽  
Salmonella Spp ◽  
Zones Of Inhibition ◽  
And Staphylococcus Aureus

Chromatographic separation of an ethyl acetate extract from Embelia schimperi led to the isolation of a new compound identified as 2,5-dihydroxy-3-methyl-1,4-benzoquinone (1) on the basis of spectroscopic and physical data. The plant’s crude extract and pure compound 1 were assayed for in vitro antimicrobial activity against clinical strains of Salmonella spp., Proteus spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae and Staphylococcus aureus. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antimicrobial activity. Standard antibiotics namely: augmentin, cotrimoxazole, gentamycin, tetracycline and lyncomycin were used as controls. The crude extract was inactive while the pure compound 1 showed significant activities against Salmonella spp., Proteus spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae and Staphylococcus aureus with zones of inhibition ranging from 10−20 mm. The most sensitive microorganism was P. aeruginosa while C. neoformans was insensitive to both the crude extract and compound 1.

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