scholarly journals Genetic Basis of Tetracycline and Minocycline Resistance in Potentially Probiotic Lactobacillus plantarum Strain CCUG 43738

2006 ◽  
Vol 50 (4) ◽  
pp. 1550-1551 ◽  
Author(s):  
Geert Huys ◽  
Klaas D'Haene ◽  
Jean Swings

ABSTRACT The potentially probiotic strain Lactobacillus plantarum CCUG 43738, which displayed atypical phenotypic resistance to tetracycline (MIC, 512 μg/ml) and minocycline (MIC, 256 μg/ml), was found to contain a tet(S) gene located on a plasmid of approximately 14 kb. Plasmid curing with novobiocin eliminated this plasmid and restored the tetracycline-susceptible phenotype of the host strain.

2015 ◽  
Vol 6 (3) ◽  
pp. 233-243 ◽  
Author(s):  
P. Hütt ◽  
E. Songisepp ◽  
M. Rätsep ◽  
R. Mahlapuu ◽  
K. Kilk ◽  
...  

The blood pressure-lowering effect of dairy products holds the potential to decrease the risk of cardiovascular disease (CVD). An open question is if the successful expression of functional properties of the probiotic strain depends on host biomarkers and/or food matrix properties. The probiotic Lactobacillus plantarum strain TENSIA® (DSM 21380) is a novel microorganism with antimicrobial and antihypertensive functional properties. The aim of this study was to characterise the functional properties of the probiotic L. plantarum TENSIA and compare its effects on host anthropometric, clinical, and blood biomarkers when consumed with cheese or yoghurt. This study involved two double-blinded randomised placebo-controlled exploratory trials (ISRCTN15061552 and ISRCTN79645828) of healthy adults over a three-week period. The three-week consumption of probiotic L. plantarum TENSIA in a daily dose of 1×1010 cfu in probiotic cheese or a daily dose of 6×109 cfu in yoghurt with different content of carbohydrates, proteins, and lipids did not significantly change the body mass index (BMI), plasma glucose and lipid levels, or inflammatory markers in the blood. Reduced lowered systolic and diastolic blood pressure values were detected, regardless of food matrix or baseline values for blood pressure and BMI. In conclusion, our study showed that three-week consumption of the probiotic L. plantarum TENSIA either in cheese or yoghurt lowered diastolic and systolic blood pressure regardless of food matrix and baseline values of blood pressure and BMI, confirming the impact of the functional properties of the probiotic strain in decreasing CVD risk.


2017 ◽  
Vol 06 (11) ◽  
Author(s):  
Golnoush Madani ◽  
Maryam Mirlohi ◽  
Sabiheh Soleimanain-Zad ◽  
Parham Hosseini ◽  
Mina Babashahi

Author(s):  
Louella Concepta Goveas ◽  
K.S. Ashwath ◽  
Brejeth Renita Nazerath ◽  
Ostrin Dsouza ◽  
Ullekh ◽  
...  

2010 ◽  
Vol 193 (1) ◽  
pp. 313-314 ◽  
Author(s):  
Y. Wang ◽  
C. Chen ◽  
L. Ai ◽  
F. Zhou ◽  
Z. Zhou ◽  
...  

2021 ◽  
Vol 11 (13) ◽  
pp. 5765
Author(s):  
Joo-Yun Kim ◽  
Eun-Jung Choi ◽  
Jae-Ho Lee ◽  
Myeong-Seok Yoo ◽  
Keon Heo ◽  
...  

Vitamin B2, also known as riboflavin, is essential for maintaining human health. The purpose of this study was to isolate novel lactic acid bacteria that overproduce vitamin B2 and to validate their potential as probiotics. In this study, Lactobacillus plantarum HY7715 (HY7715) was selected among lactic acid bacteria isolated from Kimchi. HY7715 showed a very high riboflavin-producing ability compared to the control strain due to the high expression of ribA, ribB, ribC, ribH, and ribG genes. HY7715 produced 34.5 ± 2.41 mg/L of riboflavin for 24 h without consuming riboflavin in the medium under optimal growth conditions. It was able to produce riboflavin in an in vitro model of the intestinal environment. In addition, when riboflavin deficiency was induced in mice through nutritional restriction, higher levels of riboflavin were detected in plasma and urine in the HY7715 administration group than in the control group. HY7715 showed high survival rate in simulated gastrointestinal conditions and had antibiotic resistance below the cutoff MIC value suggested by the European Food Safety Authority; moreover, it did not cause hemolysis. In conclusion, HY7715 could be considered a beneficial probiotic strain for human and animal applications, suggesting that it could be a new alternative to address riboflavin deficiency.


2015 ◽  
Vol 53 (1) ◽  
pp. 766-774 ◽  
Author(s):  
Darko Dimitrovski ◽  
Elena Velickova ◽  
Maja Dimitrovska ◽  
Tomaz Langerholc ◽  
Eleonora Winkelhausen

2018 ◽  
Vol 7 (19) ◽  
Author(s):  
Sandra Rayen Quilodran-Vega ◽  
Leonardo Albarracin ◽  
Elvira Maria Hebert ◽  
Lucila Saavedra ◽  
Alexis Fonseca ◽  
...  

This report describes the draft genome sequence of Lactobacillus brevis TUCO-5E, a probiotic strain isolated from porcine maternal milk. The reads were generated by a whole-genome sequencing (WGS) strategy on an Illumina MiSeq sequencer and were assembled into contigs with a total estimated size of 2,461,089 bp.


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