scholarly journals Environmental Stress and Antibiotic Resistance in Food-Related Pathogens

2006 ◽  
Vol 73 (1) ◽  
pp. 211-217 ◽  
Author(s):  
M. Ann S. McMahon ◽  
Jiru Xu ◽  
John E. Moore ◽  
Ian S. Blair ◽  
David A. McDowell
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Food Preservation ◽  
Important Food ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Sublethal Stress ◽  
Serovar Typhimurium ◽  
High Or Low Temperature

ABSTRACT This study investigated the possibility that sublethal food preservation stresses (high or low temperature and osmotic and pH stress) can lead to changes in the nature and scale of antibiotic resistance (ABR) expressed by three food-related pathogens (Escherichia coli, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus). The study found that some sublethal stresses significantly altered antibiotic resistance. Incubation at sublethal high temperature (45°C) decreased ABR. Incubation under increased salt (>4.5%) or reduced pH (<5.0) conditions increased ABR. Some of the pathogens continued to express higher levels of ABR after removal of stress, suggesting that in some cases the applied sublethal stress had induced stable increases in ABR. These results indicate that increased use of bacteriostatic (sublethal), rather than bactericidal (lethal), food preservation systems may be contributing to the development and dissemination of ABR among important food-borne pathogens.

scholarly journals Antibiotic resistance patterns of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella and Vibrio isolated from chicken, pork, buffalo and goat meat in eastern Nepal

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Kamana Bantawa ◽  
Shiv Nandan Sah ◽  
Dhiren Subba Limbu ◽  
Prince Subba ◽  
Arjun Ghimire
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Resistance Patterns ◽  
Meat Samples

Abstract Objective Food-borne pathogens are a major cause of illnesses, death and expenses. Their occurrence in meat and other food is considered a global health problem. The burden of food-borne disease is increasing due to antimicrobial resistance which represents a greater risk of treatment failure. However, very little is known about the antibiotic resistance profile of food-borne pathogens in Nepal. This study was conducted to examine the antibiotic resistance profile of common food-borne bacterial pathogens isolated from raw meat sold in Nepal. A total of 83 meat samples were collected from the market and analyzed. Results The prevalence of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella, and Vibrio were 68%, 53%, 35%, 6%, and 6% respectively. The resistance of Salmonella was most frequently observed to amoxicillin (100%), tetracycline (24%), chloramphenicol (11%), and nalidixic acid (11%). S. aureus was resistant to amoxicillin (100%) followed by tetracycline (63%), nalidixic acid (17%), and cefotaxime (13%) respectively. Vibrio isolates resisted amoxicillin (100%), tetracycline (40%) and chloramphenicol (20%). Shigella expressed the highest resistance to amoxicillin (100%), followed by chloramphenicol (80%), tetracycline (60%) and nalidixic acid (20%). E. coli exhibited the highest resistance to amoxicillin (100%), followed by tetracycline (93%), nalidixic acid (25%) and cefotaxime (19%).

scholarly journals Enterohemorrhagic Escherichia coli Exploits EspA Filaments for Attachment to Salad Leaves

2008 ◽  
Vol 74 (9) ◽  
pp. 2908-2914 ◽  
Author(s):  
Robert K. Shaw ◽  
Cedric N. Berger ◽  
Bart Feys ◽  
Stuart Knutton ◽  
Mark J. Pallen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Protein Translocation ◽  
Secretion System ◽  
Enterohemorrhagic Escherichia Coli ◽  
Effector Protein ◽  
Important Food ◽  
Food Borne Pathogens ◽  
Gut Epithelium ◽  
Food Borne

ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) strains are important food-borne pathogens that use a filamentous type III secretion system (fT3SS) for colonization of the gut epithelium. In this study we have shown that EHEC O157 and O26 strains use the fT3SS apparatus for attachment to leaves. Leaf attachment was independent of effector protein translocation.

Occurrence of antibiotic resistant Escherichia coli and Staphylococcus aureus in streetvended beverages accessible in an industrial zone of Bangladesh

2021 ◽  
Author(s):  
Mueena Jahan ◽  
S. M. Mostafizur Rahaman Sumon ◽  
Abu Sadeque Md. Selim ◽  
Md. Morshedur Rahman
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Bacterial Load ◽  
Resistance Pattern ◽  
Public Health Issue ◽  
Microbial Count ◽  
Industrial Zone ◽  
Food Borne ◽  
And Staphylococcus Aureus

The street-vended fruit beverages are considered as a source of disease causing microorganisms despite their high consumer demand and nutritional value. The study was conducted for the assessment of antibiotic resistance food-borne pathogens in street-vended fruit beverages available in an industrial zone of Bangladesh. A total of 50 samples constituting five different types of street-vended fruit beverages were collected from Gazipur city of Bangladesh. All the samples were examined for total viable microbial count followed by isolation, identification and antibiogram profile of Escherichia coli and Staphylococcus aureus. The total viable microbial count ranged from 2.3 to 8.9 log cfu/ml suggesting a significant bacterial load that exceeds the maximum permitted level of microbial load for freshly extracted fruit juices. Of the 50 samples, 29 (58.0%) were appeared as Staphylococcus aureus positive and 24 (48.0%) samples were contaminated with Escherichia coli. Antibiogram profile revealed Staphylococcus aureus resistance to penicillin (82.8%), amoxicillin (75.9%) and oxacillin (17.2%). The resistance to β-lactam antibiotic like oxacillin indicates the presence of highly pathogenic Staphylococcus aureus which is obviously a potent public health issue. In contrary, Escherichia coli isolates were resistant to amoxicillin (100%) and erythromycin (100%). All the isolates were found highly susceptible to ciprofloxacin and gentamicin. The antibiotic resistance pattern of the isolated organisms could complicate the treatment of food-borne illness in people. Development of holistic approach and their effective application could ensure the hygienic quality of these beverages as well as the health standard of the consumers.

scholarly journals The extracellular juncture domains in the intimin passenger adopt a constitutively extended conformation inducing restraints to its sphere of action

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Julia Weikum ◽  
Alina Kulakova ◽  
Giulio Tesei ◽  
Shogo Yoshimoto ◽  
Line Vejby Jægerum ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Global Health ◽  
Host Cell ◽  
Virulence Factor ◽  
Important Food ◽  
Extracellular Medium ◽  
E Coli ◽  
Food Borne Pathogens ◽  
Medium Interface ◽  
Food Borne

AbstractEnterohemorrhagic and enteropathogenic Escherichia coli are among the most important food-borne pathogens, posing a global health threat. The virulence factor intimin is essential for the attachment of pathogenic E. coli to the intestinal host cell. Intimin consists of four extracellular bacterial immunoglobulin-like (Big) domains, D00–D2, extending into the fifth lectin subdomain (D3) that binds to the Tir-receptor on the host cell. Here, we present the crystal structures of the elusive D00–D0 domains at 1.5 Å and D0–D1 at 1.8 Å resolution, which confirms that the passenger of intimin has five distinct domains. We describe that D00–D0 exhibits a higher degree of rigidity and D00 likely functions as a juncture domain at the outer membrane-extracellular medium interface. We conclude that D00 is a unique Big domain with a specific topology likely found in a broad range of other inverse autotransporters. The accumulated data allows us to model the complete passenger of intimin and propose functionality to the Big domains, D00–D0–D1, extending directly from the membrane.

Introduction to the Food Safety Concerns of Verotoxin-Producing Escherichia coli

2003 ◽  
Vol 228 (4) ◽  
pp. 331-332 ◽  
Author(s):  
Hussein S. Hussein ◽  
Stanley T. Omaye
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Safety Concern ◽  
Critical Evaluation ◽  
The United States ◽  
Global Perspective ◽  
E Coli ◽  
The Past ◽  
Food Borne Pathogens ◽  
Food Borne

Verotoxin-producing Escherichia coli (VTEC) have emerged in the past two decades as food-borne pathogens that can cause major outbreaks of human illnesses worldwide. The number of outbreaks has increased in recent years due to changes in food production and processing systems, eating habits, microbial adaptation, and methods of VTEC transmission. The human illnesses range from mild diarrhea to hemolytic uremic syndrome (HUS) that can lead to death. The VTEC outbreaks have been attributed to O157:H7 and non-O157:H7 serotypes of E. coli. These E. coli serotypes include motile (e.g., O26:H11 and O104:H21) and nonmotile (e.g., O111:H–,0145:H–, and O157:H–) strains. In the United States, E. coli O157:H7 has been the major cause of VTEC outbreaks. Worldwide, however, non-O157:H7 VTEC (e.g., members of the 026, O103, O111, O118, O145, and O166 serogroups) have caused approximately 30% of the HUS cases in the past decade. Because large numbers of the VTEC outbreaks have been attributed to consumption of ruminant products (e.g., ground beef), cattle and sheep are considered reservoirs of these food-borne pathogens. Because of the food safety concern of VTEC, a global perspective on this problem is addressed (Exp Biol Med Vol. 228, No. 4). The first objective was to evaluate the known non-O157:H7 VTEC strains and the limitations associated with their detection and characterization. The second objective was to identify the VTEC serotypes associated with outbreaks of human illnesses and to provide critical evaluation of their virulence. The third objective was to determine the rumen effect on survival of E. coli O157:H7 as a VTEC model. The fourth objective was to explore the role of intimins in promoting attaching and effacing lesions in humans. Finally, the ability of VTEC to cause persistent infections in cattle was evaluated.

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