scholarly journals The Genome Sequence of the Crenarchaeon Acidilobus saccharovorans Supports a New Order, Acidilobales, and Suggests an Important Ecological Role in Terrestrial Acidic Hot Springs

2010 ◽  
Vol 76 (16) ◽  
pp. 5652-5657 ◽  
Author(s):  
Andrey V. Mardanov ◽  
Vitali A. Svetlitchnyi ◽  
Alexey V. Beletsky ◽  
Maria I. Prokofeva ◽  
Elizaveta A. Bonch-Osmolovskaya ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Hot Springs ◽  
Hot Spring ◽  
Tricarboxylic Acid Cycle ◽  
Proton Translocation ◽  
New Order ◽  
Organic Substrates ◽  
Transfer Chain ◽  
Terrestrial Hot Spring

ABSTRACT Acidilobus saccharovorans is an anaerobic, organotrophic, thermoacidophilic crenarchaeon isolated from a terrestrial hot spring. We report the complete genome sequence of A. saccharovorans, which has permitted the prediction of genes for Embden-Meyerhof and Entner-Doudoroff pathways and genes associated with the oxidative tricarboxylic acid cycle. The electron transfer chain is branched with two sites of proton translocation and is linked to the reduction of elemental sulfur and thiosulfate. The genomic data suggest an important role of the order Acidilobales in thermoacidophilic ecosystems whereby its members can perform a complete oxidation of organic substrates, closing the anaerobic carbon cycle.

scholarly journals Carbon Source Preference in Chemosynthetic Hot Spring Communities

2015 ◽  
Vol 81 (11) ◽  
pp. 3834-3847 ◽  
Author(s):  
Matthew R. Urschel ◽  
Michael D. Kubo ◽  
Tori M. Hoehler ◽  
John W. Peters ◽  
Eric S. Boyd
Keyword(s):  
High Temperature ◽  
National Park ◽  
Hot Springs ◽  
Dissolved Inorganic Carbon ◽  
Hot Spring ◽  
Affinity Constant ◽  
Rrna Gene ◽  
Organic Substrates ◽  
Short Term ◽  
Acetate Assimilation

ABSTRACTRates of dissolved inorganic carbon (DIC), formate, and acetate mineralization and/or assimilation were determined in 13 high-temperature (>73°C) hot springs in Yellowstone National Park (YNP), Wyoming, in order to evaluate the relative importance of these substrates in supporting microbial metabolism. While 9 of the hot spring communities exhibited rates of DIC assimilation that were greater than those of formate and acetate assimilation, 2 exhibited rates of formate and/or acetate assimilation that exceeded those of DIC assimilation. Overall rates of DIC, formate, and acetate mineralization and assimilation were positively correlated with spring pH but showed little correlation with temperature. Communities sampled from hot springs with similar geochemistries generally exhibited similar rates of substrate transformation, as well as similar community compositions, as revealed by 16S rRNA gene-tagged sequencing. Amendment of microcosms with small (micromolar) amounts of formate suppressed DIC assimilation in short-term (<45-min) incubations, despite the presence of native DIC concentrations that exceeded those of added formate by 2 to 3 orders of magnitude. The concentration of added formate required to suppress DIC assimilation was similar to the affinity constant (Km) for formate transformation, as determined by community kinetic assays. These results suggest that dominant chemoautotrophs in high-temperature communities are facultatively autotrophic or mixotrophic, are adapted to fluctuating nutrient availabilities, and are capable of taking advantage of energy-rich organic substrates when they become available.

FeAs2 biomineralization on encrusted bacteria in hot springs: an ecological role of symbiotic bacteria

2003 ◽  
Vol 40 (11) ◽  
pp. 1725-1738 ◽  
Author(s):  
Kazue Tazaki ◽  
Islam ABM Rafiqul ◽  
Kaori Nagai ◽  
Takayuki Kurihara
Keyword(s):  
Metal Binding ◽  
Microbial Mats ◽  
Hot Springs ◽  
Hot Spring ◽  
Binding Potential ◽  
Resistant Bacteria ◽  
X Ray Diffraction ◽  
Ft Ir ◽  
Ir Analysis

Bacterial FeAs2 mineralization was found in the reddish-brown microbial mats that have grown on the walls of the drainage systems of Masutomi Hot Springs, Yamanashi Prefecture, Japan. The reddish-brown microbial mats, which are mainly composed of bacilliform and coccoid types of bacteria, have been analyzed and observed by microtechniques to interpret the bacterial biomineralization and search for the clues to bioremediation. These bacteria accumulate Fe and As along with other trace elements to form various biominerals. The electron diffraction (ED) pattern of the bacterial capsule identified lollingite (FeAs2) and calcite (CaCO3) on the surface of the cell. Based on Fourier-transform infrared absorbance spectroscopy (FT–IR) analysis, the presence of organic components such as C—H, C=O, CNH, –COOH, and N—H in the reddish-brown microbial mats emphasized the metal-binding potential of the bacteria. X-ray diffraction (XRD) data showed the poorly crystalline character of the precipitates, which consist mainly of hydrous iron oxides (2.7 Å (1 Å = 0.1 nm)). The FeAs2 biominerals form by adsorption onto the bacterial cell wall, as demonstrated by microscopic observations and spectroscopic analysis. These showed that bacteria in the reddish-brown microbial mats have the ability to form biominerals with heavy metals and toxic metalloids like As. Particularly significant in hot spring environments is the role of symbiotic and toxic-resistant bacteria, which have the ability to adapt to high As concentrations. Bacterial FeAs2 mineralization might also be considered a mechanism by which toxic As is removed from the aquatic ecosystem. The results provide evidence for detoxification processes and offer clues to possible methods of bioremediation.

Searching memories of pleasures in local cuisine: how nostalgia and hedonic values affect tourists' behavior at hot spring destinations?

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Janet Chang ◽  
Sean Hsin-Hung Lin ◽  
Li-Sheng Wu
Keyword(s):  
Food Consumption ◽  
Structural Equation ◽  
Hot Springs ◽  
Local Food ◽  
Hot Spring ◽  
Equation Modeling ◽  
Content Type ◽  
Mediating Role ◽  
Hedonic Experience

PurposeHistorically, hot springs have been regarded as hedonic and foodie destinations, yet the antecedents that affect tourists' intentions for local cuisine in hot springs remain unexplored. The present study aims to address this knowledge gap by considering the role of nostalgia and hedonic values in tourist food consumption.Design/methodology/approachData were collected from 315 domestic tourists by intercept surveys conducted in the Beitou Hot Spring, Taiwan. Covariance-based structural equation modeling (SEM) and bootstrapping were used to test the hypotheses as well as mediating effects.FindingsNostalgia positively influences hedonic values, which affect tourists' intentions for local food consumption. Unexpectedly, nostalgia does not directly influence tourists' preferences due to complete mediation through hedonic values.Research limitations/implicationsGiven a growing number of young consumers visiting hot springs, hedonic experience is essential and more effective for pulling visitors and stimulating local food consumption than nostalgia vibes are. Cross-cultural samples and qualitative research are suggested for future studies.Originality/valueThe study demonstrates different levels of nostalgia in different ages. It highlights the mediating role of hedonic values between nostalgia and tourists' intentions for local cuisine in the hot spring destinations, which has been overlooked in previous studies. Originality is also established by simultaneously investigating hedonic values and behavioral intentions within the context of food tourism.

scholarly journals Draft Genome Sequence of a Divergent Anaerobic Member of the Chloroflexi Class Ardenticatenia from a Sulfidic Hot Spring

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
L. M. Ward ◽  
S. E. McGlynn ◽  
W. W. Fischer
Keyword(s):  
Genome Sequence ◽  
New Genus ◽  
Hot Spring ◽  
Draft Genome ◽  
High Potential ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Family Level ◽  
Terrestrial Hot Spring

Here, we present a draft genome sequence of Nak82, the second genome sequence available for the Chloroflexi class Ardenticatenia and the first from a sulfidic terrestrial hot spring. Nak82 is genetically and metabolically distinct from Ardenticatena maritima and likely represents a new genus- or family-level lineage lacking high-potential respiratory pathways.

scholarly journals Draft Genome Sequence of Tepidiphilus thermophilus Strain JHK30 T (JCM 19170 T ) Isolated from a Terrestrial Hot Spring in India

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Abhijit Poddar ◽  
Rinchen T. Lepcha ◽  
William B. Whitman ◽  
Subrata K. Das
Keyword(s):  
Genome Sequence ◽  
Hot Spring ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Protein Coding ◽  
Coding Sequences ◽  
Gram Negative ◽  
Terrestrial Hot Spring

Tepidiphilus thermophilus strain JHK30 T was isolated from a hot spring at Surajkund, Jharkhand, India. It is a Gram-negative rod, nonsporulating, aerobic, and motile. The estimated genome is 2.3 Mb, with 2,186 protein-coding sequences.

scholarly journals Meet me in the middle: median temperatures impact cyanobacteria and photoautotrophy in eruptive Yellowstone hot springs

2021 ◽  
Author(s):  
Trinity L. Hamilton ◽  
Jeff Havig
Keyword(s):  
High Temperature ◽  
Community Composition ◽  
Hot Springs ◽  
Hot Spring ◽  
Temperature Fluctuations ◽  
Model Systems ◽  
Large Temperature ◽  
Ecological Diversification ◽  
Outflow Channels

AbstractGeographic isolation can be a main driver of microbial evolution in hot springs while temperature plays a role on local scales. For example, cyanobacteria, particularly high temperature Synechococcus spp., have undergone ecological diversification along temperature gradients in hot spring outflow channels. While water flow, and thus temperature, is largely stable in many hot springs, flow can vary in geysing/eruptive hot springs resulting in large temperature fluctuations (sometimes more than 40°C). However, the role of large temperature fluctuations in driving diversification of cyanobacteria in eruptive hot springs has not been explored. Here, we examined phototroph community composition and potential photoautotrophic activity in two alkaline eruptive hot springs with similar geochemistry in the Lower Geyser Basin in Yellowstone National Park, WY. We observed distinct cyanobacterial amplicon sequencing variants (ASVs) consistent with allopatry and levels of light-dependent inorganic carbon uptake rates similar to other hot springs, despite large temperature fluctuations. Our data suggests median temperatures may drive phototroph fitness in eruptive hot springs while future studies are necessary to determine the evolutionary consequences of thriving under continuously fluctuating temperatures. We propose that large temperature swings in eruptive hot springs offer unique environments to examine the role of allopatry vs. physical and chemical characteristics of ecosystems in driving cyanobacteria evolution and add to the debate regarding the ecology of thermal adaptation and the potential for narrowing niche breadth with increasing temperature.ImportanceHot spring cyanobacteria have long been model systems for examining ecological diversification as well as characterizing microbial adaptation and evolution to extreme environments. These studies have reported cyanobacterial diversification in hot spring outflow channels that can be defined by distinct temperature ranges. Our study builds on these previous studies by examining cyanobacteria in geysing hot springs. Geysing hot springs result in outflow channel that experience regular and large temperature fluctuations. While community composition is similar between geysing and nongeysing hot spring outflow channels, our data suggests median, rather than high temperature, drive the fitness of cyanobacteria in geysing hot springs. We propose that large temperature swings may result in patterns of ecological diversification that are distinct from more stable outflows.

Phototrophs in High-Iron-Concentration Microbial Mats: Physiological Ecology of Phototrophs in an Iron-Depositing Hot Spring

1999 ◽  
Vol 65 (12) ◽  
pp. 5474-5483 ◽  
Author(s):  
B. K. Pierson ◽  
M. N. Parenteau ◽  
B. M. Griffin
Keyword(s):  
Oxygen Concentration ◽  
Ferrous Iron ◽  
Hot Springs ◽  
Hot Spring ◽  
Suitable Model ◽  
Visible Radiation ◽  
Dark Light ◽  
High Iron ◽  
The Impact

ABSTRACT At Chocolate Pots Hot Springs in Yellowstone National Park the source waters have a pH near neutral, contain high concentrations of reduced iron, and lack sulfide. An iron formation that is associated with cyanobacterial mats is actively deposited. The uptake of [14C]bicarbonate was used to assess the impact of ferrous iron on photosynthesis in this environment. Photoautotrophy in some of the mats was stimulated by ferrous iron (1.0 mM). Microelectrodes were used to determine the impact of photosynthetic activity on the oxygen content and the pH in the mat and sediment microenvironments. Photosynthesis increased the oxygen concentration to 200% of air saturation levels in the top millimeter of the mats. The oxygen concentration decreased with depth and in the dark. Light-dependent increases in pH were observed. The penetration of light in the mats and in the sediments was determined. Visible radiation was rapidly attenuated in the top 2 mm of the iron-rich mats. Near-infrared radiation penetrated deeper. Iron was totally oxidized in the top few millimeters, but reduced iron was detected at greater depths. By increasing the pH and the oxygen concentration in the surface sediments, the cyanobacteria could potentially increase the rate of iron oxidation in situ. This high-iron-content hot spring provides a suitable model for studying the interactions of microbial photosynthesis and iron deposition and the role of photosynthesis in microbial iron cycling. This model may help clarify the potential role of photosynthesis in the deposition of Precambrian banded iron formations.

Fontimonas thermophila gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a freshwater hot spring, and proposal of Solimonadaceae fam. nov. to replace Sinobacteraceae Zhou et al. 2008

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 254-259 ◽  
Author(s):  
Nathaniel A. Losey ◽  
Bradley S. Stevenson ◽  
Susanne Verbarg ◽  
Stephen Rudd ◽  
Edward R. B. Moore ◽  
...  
Keyword(s):  
Type Species ◽  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
New Family ◽  
Terrestrial Hot Spring

A novel bacterial strain designated HA-01T was isolated from a freshwater terrestrial hot spring located at Hot Springs National Park, Arkansas, USA. Cells were Gram-negative-staining, rod-shaped, aerobic, chemo-organotrophic, oxidase- and catalase-positive, non-spore-forming and motile by means of a single polar flagellum. Growth occurred at 37–60 °C, with an optimum between 45 and 50 °C, and at pH 6.5–8.5, with an optimum between pH 6.5 and 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of strain HA-01T were Solimonas aquatica NAA-16T (93.8 %), Solimonas flava CW-KD 4T (94.1 %), Solimonas soli DCY12T (93.1 %), Solimonas variicoloris MN28T (94.0 %), Nevskia ramosa Soe1T (91.2 %) and Hydrocarboniphaga effusa AP103T (91.1 %). Major fatty acids consisted of C16 : 0, iso-C16 : 0, C16 : 1ω5c and summed feature 8 (C18 : 1ω9c, C18 : 1ω7c and C18 : 1ω6c). Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major isoprenoid quinone was Q-8. The DNA G+C content was 64.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, it is proposed that strain HA-01T represents a novel species in a new genus for which the name Fontimonas thermophila gen. nov., sp. nov. is proposed. The type strain of the type species is HA-01T ( = DSM 23609T = CCUG 59713T). A new family, Solimonadaceae fam. nov., is also proposed to replace Sinobacteriaceae Zhou et al. 2008.

Mereka yang Tidak Dibayar Tinggi: Ibuisme, Taman Kanak-kanak, dan Kampung di Indramayu

2018 ◽  
Vol 3 (2) ◽  
pp. 121
Author(s):  
Mochammad Arief Wicaksono
Keyword(s):  
Kindergarten Teachers ◽  
New Order ◽  
The Family ◽  
Role Of Women ◽  
Community Pressure ◽  
The Village ◽  
Depth Interviews ◽  
The Ideal

The ideology of state-ibuism has always been interwoven with how the New Order regime until nowadays government constructing the “ideal” role of women in the family and community through the PKK (Pembinaan Kesejahteraan Keluarga) organization. However, in Cangkring Village, Indramayu, the ideology of ibuism works not because of the massive government regulating the role of women through the PKK organization, but it is possible because of the structure of the kampung community itself. Through involved observations and in-depth interviews about a kindergarten in the village, a group of housewives who dedicated themselves to teaching in kindergarten were met without getting paid high. From these socio-cultural phenomenons, this paper will describe descriptively and analytically that housewives in the Cangkring village are willing to become kindergarten teachers because of their moral burden as part of the warga kampung and also from community pressure from people who want their children to be able to read and write.

KEGAGALAN PRIVATISASI BUMN DI ERA REZIM ORDE BARU DALAM PERSPEKTIF EKONOMI POLITIK

Wacana Publik ◽  
2019 ◽  
Vol 12 (02) ◽  
Author(s):  
Syamsul Ma'arif
Keyword(s):  
Market Economy ◽  
Free Market ◽  
Dominant Role ◽  
The State ◽  
Early Years ◽  
New Order ◽  
Free Market Economy ◽  
Economic Sectors ◽  
Role Of The State

After had being carried out nationalization and hostility against west countries, the New Order regime made important decision to change Indonesia economic direction from etatism system to free market economy. A set of policies were taken in order private sector could play major role in economic. However, when another economic sectors were reformed substantially, effords to reform the State Owned Enterprises had failed. The State Owned Enterprise, in fact, remained to play dominant role like early years of guided democracy era. Role of the State Owned Enterprises was more and more powerfull). The main problem of reforms finally lied on reality that vested interest of bureaucrats (civil or military) was so large that could’nt been overcome. 

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