scholarly journals The Intestinal Microbiota of Hermetia illucens Larvae Is Affected by Diet and Shows a Diverse Composition in the Different Midgut Regions

2018 ◽  
Vol 85 (2) ◽  
Author(s):  
Daniele Bruno ◽  
Marco Bonelli ◽  
Francesca De Filippis ◽  
Ilaria Di Lelio ◽  
Gianluca Tettamanti ◽  
...  
Keyword(s):  
Anterior Part ◽  
Animal Origin ◽  
Organic Substrates ◽  
Hermetia Illucens ◽  
Efficient System ◽  
Content Type ◽  
Larval Midgut ◽  
Protein Sources ◽  
Feeding Substrate ◽  
Microbiological Aspects

ABSTRACT The larva of the black soldier fly (Hermetia illucens) has emerged as an efficient system for the bioconversion of organic waste. Although many research efforts are devoted to the optimization of rearing conditions to increase the yield of the bioconversion process, microbiological aspects related to this insect are still neglected. Here, we describe the microbiota of the midgut of H. illucens larvae, showing the effect of different diets and midgut regions in shaping microbial load and diversity. The bacterial communities residing in the three parts of the midgut, characterized by remarkable changes in luminal pH values, differed in terms of bacterial numbers and microbiota composition. The microbiota of the anterior part of the midgut showed the highest diversity, which gradually decreased along the midgut, whereas bacterial load had an opposite trend, being maximal in the posterior region. The results also showed that the influence of the microbial content of ingested food was limited to the anterior part of the midgut, and that the feeding activity of H. illucens larvae did not significantly affect the microbiota of the substrate. Moreover, a high protein content compared to other macronutrients in the feeding substrate seemed to favor midgut dysbiosis. The overall data indicate the importance of taking into account the presence of different midgut structural and functional domains, as well as the substrate microbiota, in any further study that aims at clarifying microbiological aspects concerning H. illucens larval midgut. IMPORTANCE The demand for food of animal origin is expected to increase by 2050. Since traditional protein sources for monogastric diets are failing to meet the increasing demand for additional feed production, there is an urgent need to find alternative protein sources. The larvae of Hermetia illucens emerge as efficient converters of low-quality biomass into nutritionally valuable proteins. Many studies have been performed to optimize H. illucens mass rearing on a number of organic substrates and to quantitatively and qualitatively maximize the biomass yield. On the contrary, although the insect microbiota can be fundamental for bioconversion processes and its characterization is mandatory also for safety aspects, this topic is largely overlooked. Here, we provide an in-depth study of the microbiota of H. illucens larval midgut, taking into account pivotal aspects, such as the midgut spatial and functional regionalization, as well as microbiota and nutrient composition of the feeding substrate.

Arteriovenous malformations in the posterior fossa

1977 ◽  
Vol 47 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Hiroshi Matsumura ◽  
Yasumasa Makita ◽  
Kuniyuki Someda ◽  
Akinori Kondo
Keyword(s):  
Arteriovenous Malformation ◽  
Brain Stem ◽  
Posterior Fossa ◽  
Arteriovenous Malformations ◽  
Cerebellopontine Angle ◽  
Anterior Part ◽  
Content Type ◽  
The Brain ◽  
Fine Print

✓ We have operated on 12 of 14 cases of arteriovenous malformation (AVM) in the posterior fossa since 1968, with one death. The lesions were in the cerebellum in 10 cases (three anteromedial, one central, three lateral, and three posteromedial), and in the cerebellopontine angle in two; in two cases the lesions were directly related to the brain stem. The AVM's in the anterior part of the cerebellum were operated on through a transtentorial occipital approach.

Exposure of the anterior part of the circle of Willis in the dog

1974 ◽  
Vol 41 (1) ◽  
pp. 107-112
Author(s):  
Shigeaki Hori ◽  
Williamina A. Himwich
Keyword(s):  
Anterior Part ◽  
Cerebral Arteries ◽  
Circle Of Willis ◽  
Content Type ◽  
Anatomical Characteristics ◽  
Fine Print

✓ A technique for exposing the vessels in the anterior part of the circle of Willis in the dog is described. Some of the physiological and anatomical characteristics of the anterior communicating and the anterior cerebral arteries are discussed.

Vegan cheeses vs processed cheeses – traceability issues and monitoring countermeasures

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Moawiya A. Haddad ◽  
Sharaf S. Omar ◽  
Salvatore Parisi
Keyword(s):  
Food Supply ◽  
Design Methodology ◽  
Raw Materials ◽  
Production Method ◽  
Animal Origin ◽  
Information Load ◽  
Supply Chain Risk ◽  
Supply Networks ◽  
Content Type ◽  
Traceability System

PurposeThe purpose of this study comes from the need of defining improved durability values and the realization of a good traceability management for selected vegan cheeses has suggested the comparison between a processed cheese and its analogous version without animal-origin raw materials. The durability should be studied at a well-defined temperature, probably agreed among the food producer and the food processor. In addition, the traceability system should consider many components and related suppliers.Design/methodology/approachA supply chain risk assessment analysis has been carried out with relation to two different products: an analogue cheese and a vegan cheese-like preparation. Raw materials and ingredients have been evaluated (production method and origin; geographical identification), with the aim of identifying simplified food.FindingsAn assessment of food supply networks has been carried out. In the first situation (analogue cheeses), the ingredient “cheeses” shows an important complexity: five suppliers with a related six-interconnection hub. On the other side, vegan cheeses are obtained from 11 ingredients (a challenging hub); four of them may be produced from 2–5 components of different origin (five total hubs). Tested processed cheeses are represented by means of a linear food supply network with two hubs (cheeses and “arrival” show degrees 6 and 9, respectively). Networks concerning vegan cheeses include five different hubs: four complex raw materials (degree: 2, 3, 4 and 5) and the “arrival” step (degree: 12).Originality/valueThe information load of vegan cheeses (two hubs, degrees >> average degree) appears high if compared with processed cheeses (two hubs), although the complexity of networks appears similar. Vegan cheeses may seem technologically simpler than processed cheeses and be sometimes questioned because of important traceability issues. Adequate traceability countermeasures in terms of preventive monitoring actions should be recommended when speaking of vegan cheeses. Anyway, a centralized manager would be always required.

scholarly journals Emergence of Epidemic Multidrug-Resistant Enterococcus faecium from Animal and Commensal Strains

mBio ◽  
2013 ◽  
Vol 4 (4) ◽  
Author(s):  
François Lebreton ◽  
Willem van Schaik ◽  
Abigail Manson McGuire ◽  
Paul Godfrey ◽  
Allison Griggs ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Sequence Data ◽  
Mobile Element ◽  
Multidrug Resistant ◽  
Human Infection ◽  
Animal Origin ◽  
Loss Of Function ◽  
Content Type ◽  
Hospital Acquired Infection ◽  
Hospital Acquired

ABSTRACTEnterococcus faecium, natively a gut commensal organism, emerged as a leading cause of multidrug-resistant hospital-acquired infection in the 1980s. As the living record of its adaptation to changes in habitat, we sequenced the genomes of 51 strains, isolated from various ecological environments, to understand howE. faeciumemerged as a leading hospital pathogen. Because of the scale and diversity of the sampled strains, we were able to resolve the lineage responsible for epidemic, multidrug-resistant human infection from other strains and to measure the evolutionary distances between groups. We found that the epidemic hospital-adapted lineage is rapidly evolving and emerged approximately 75 years ago, concomitant with the introduction of antibiotics, from a population that included the majority of animal strains, and not from human commensal lines. We further found that the lineage that included most strains of animal origin diverged from the main human commensal line approximately 3,000 years ago, a time that corresponds to increasing urbanization of humans, development of hygienic practices, and domestication of animals, which we speculate contributed to their ecological separation. Each bifurcation was accompanied by the acquisition of new metabolic capabilities and colonization traits on mobile elements and the loss of function and genome remodeling associated with mobile element insertion and movement. As a result, diversity within the species, in terms of sequence divergence as well as gene content, spans a range usually associated with speciation.IMPORTANCEEnterococci, in particular vancomycin-resistantEnterococcus faecium, recently emerged as a leading cause of hospital-acquired infection worldwide. In this study, we examined genome sequence data to understand the bacterial adaptations that accompanied this transformation from microbes that existed for eons as members of host microbiota. We observed changes in the genomes that paralleled changes in human behavior. An initial bifurcation within the species appears to have occurred at a time that corresponds to the urbanization of humans and domestication of animals, and a more recent bifurcation parallels the introduction of antibiotics in medicine and agriculture. In response to the opportunity to fill niches associated with changes in human activity, a rapidly evolving lineage emerged, a lineage responsible for the vast majority of multidrug-resistantE. faeciuminfections.

scholarly journals High Protein Substitutes for Gluten in Gluten-Free Bread

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1997
Author(s):  
Adriana Skendi ◽  
Maria Papageorgiou ◽  
Theodoros Varzakas
Keyword(s):  
Protein Digestibility ◽  
High Protein ◽  
Animal Origin ◽  
Soluble Carbohydrates ◽  
Gluten Free ◽  
Key Factors ◽  
Free Products ◽  
Protein Sources ◽  
Come Into The Market ◽  
Careful Design

Gluten-free products have come into the market in order to alleviate health problems such as celiac disease. In this review, recent advances in gluten-free bread are described along with plant-based gluten-free proteins. A comparison with animal-based gluten-free proteins is made reporting on different high protein sources of animal origin. Sea microorganisms- and insect-based proteins are also mentioned, and the optimization of the structure of gluten-free bread with added high protein sources is highlighted along with protein digestibility issues. The latter is an issue for consideration that can be manipulated by a careful design of the mixture in terms of phenolic compounds, soluble carbohydrates and fibres, but also the baking process itself. Additionally, the presence of enzymes and different hydrocolloids are key factors controlling quality features of the final product.

Limiting vs. diversifying patterns of recommendations for key protein sources emerging: a study on national food guides worldwide from a health and sustainability perspective

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Joop de Boer ◽  
Harry Aiking
Keyword(s):  
Animal Protein ◽  
Dietary Guidelines ◽  
Food Sources ◽  
Content Type ◽  
Protein Sources ◽  
Adult Men ◽  
Health And Nutrition ◽  
Sustainable Diet ◽  
Protein Supply

PurposeA shift to a healthy and sustainable diet (as recommended by the EAT Lancet Commission) needs to have a strong societal legitimation. This makes it relevant to investigate to what extent countries are using their Food-Based Dietary Guidelines (FBDGs) in ways that can stimulate such a shift.Design/methodology/approachGiven the pivotal role of protein, the authors examined what patterns of protein-related recommendations are used to guide consumers and how these patterns are related to specific contexts and societal priorities of the countries.FindingsThe analysis of data from 93 countries worldwide revealed two emerging patterns of recommendations. One pattern (found in a cluster of 23% of the countries) combined positive advice about key protein sources with limiting messages to reduce (or replace) the consumption of animal protein. The other pattern (found in a cluster of 24%) encouraged both animal and plant protein, thereby diversifying the set of protein sources, without negative advice on animal-based food sources. The two patterns of recommendations were differently associated with health and nutrition variables, including the countries' level of animal protein supply (in particular, dairy) and the prevalence of overweight individuals among adult men.Social implicationsFor all stakeholders, it is of crucial importance to realize that an increasing number of countries in the world are moving in the direction of acknowledging and addressing the diet-health-environment nexus by adapting their patterns of recommendations for key protein sources.Originality/valueThis study is the first that reveals patterns in recommendations with respect to protein sources by different nations worldwide.

scholarly journals Identification of Antigenic Targets for Immunodetection of Balamuthia mandrillaris Infection

2011 ◽  
Vol 18 (8) ◽  
pp. 1297-1301 ◽  
Author(s):  
Zuzana Kucerova ◽  
Rama Sriram ◽  
Patricia P. Wilkins ◽  
Govinda S. Visvesvara
Keyword(s):  
Geographic Origin ◽  
Acanthamoeba Castellanii ◽  
Rapid Identification ◽  
Animal Origin ◽  
Rabbit Serum ◽  
Protein Profiles ◽  
Hyperimmune Serum ◽  
Content Type ◽  
Balamuthia Mandrillaris ◽  
Human Sera

ABSTRACTThe free-living amoebaBalamuthia mandrillariscauses granulomatous amoebic encephalitis (GAE) in humans. Rapid identification of balamuthiasis is critical for effective therapeutic intervention and case management. In the present study we identified target antigens for the development of a serological assay forB. mandrillarisinfection. We demonstrated by silver staining that protein profiles for all eight isolates ofB. mandrillaris, independent of human or animal origin or geographic origin, appeared to be similar except for some minor differences, indicating the molecular homogeneity of these strains. The profiles of all isolates, which ranged from 200 to 10 kDa, were similar, with a prominent protein visible around 30 kDa; all appeared considerably different from protein profiles of the control E6 cells andAcanthamoeba castellaniiandNaegleria fowleriisolates. Western blot analysis with rabbit hyperimmune serum identified the major immunodominant antigens of 25, 50, 75, and 80 kDa; positive human sera reacted strongly with proteins around 25, 40, 50, and 75 kDa. Proteins around 40 kDa detected by human serum were not recognized by hyperimmune rabbit serum. None of the target proteins were detected by uninfected control sera. Reactivities of five patients' sera with 4 different isolates ofB. mandrillaris(2 strains of human and 2 strains of animal origins) revealed that patients' sera reacted slightly differently with differentB. mandrillarisisolates, although major proteins of approximately 25, 50, and 75 kDa were present in all extracts.

scholarly journals Ecophysiological Distinctions of Haloarchaea from a Hypersaline Antarctic Lake as Determined by Metaproteomics

2016 ◽  
Vol 82 (11) ◽  
pp. 3165-3173 ◽  
Author(s):  
Bernhard Tschitschko ◽  
Timothy J. Williams ◽  
Michelle A. Allen ◽  
Ling Zhong ◽  
Mark J. Raftery ◽  
...  
Keyword(s):  
Low Complexity ◽  
Abundant Species ◽  
Specific Protein ◽  
Organic Substrates ◽  
Deep Lake ◽  
Nitrogen And Phosphorus ◽  
Content Type ◽  
Cold Environments ◽  
Vestfold Hills ◽  
Life On Earth

ABSTRACTDeep Lake in the Vestfold Hills is hypersaline and the coldest system in Antarctica known to support microbial growth (temperatures as low as −20°C). It represents a strong experimental model because the lake supports a low-complexity community of haloarchaea, with the three most abundant species totaling ∼72%. Moreover, the dominant haloarchaea are cultivatable, and their genomes are sequenced. Here we use metaproteomics linked to metagenome data and the genome sequences of the isolates to characterize the main pathways, trophic strategies, and interactions associated with resource utilization. The dominance of the most abundant member,Halohasta litchfieldiae, appears to be predicated on competitive utilization of substrates (e.g., starch, glycerol, and dihydroxyacetone) produced byDunaliella, the lake's primary producer, while also possessing diverse mechanisms for acquiring nitrogen and phosphorus. The second most abundant member, strain DL31, is proficient in degrading complex proteinaceous matter.Hht. litchfieldiaeand DL31 are inferred to release labile substrates that are utilized byHalorubrum lacusprofundi, the third most abundant haloarchaeon in Deep Lake. The study also linked genome variation to specific protein variants or distinct genetic capacities, thereby identifying strain-level variation indicative of specialization. Overall, metaproteomics revealed that rather than functional differences occurring at different lake depths or through size partitioning, the main lake genera possess major trophic distinctions, and phylotypes (e.g., strains ofHht. litchfieldiae) exhibit a more subtle level of specialization. This study highlights the extent to which the lake supports a relatively uniform distribution of taxa that collectively possess the genetic capacity to effectively exploit available nutrients throughout the lake.IMPORTANCELife on Earth has evolved to colonize a broad range of temperatures, but most of the biosphere (∼85%) exists at low temperatures (≤5°C). By performing unique roles in biogeochemical cycles, environmental microorganisms perform functions that are critical for the rest of life on Earth to survive. Cold environments therefore make a particularly important contribution to maintaining healthy, stable ecosystems. Here we describe the main physiological traits of the dominant microorganisms that inhabit Deep Lake in Antarctica, the coldest aquatic environment known to support life. The hypersaline system enables the growth of halophilic members of theArchaea: haloarchaea. By analyzing proteins of samples collected from the water column, we determined the functions that the haloarchaea were likely to perform. This study showed that the dominant haloarchaea possessed distinct lifestyles yet formed a uniform community throughout the lake that was collectively adept at using available light energy and diverse organic substrates for growth.

Effects of milk proteins supplementation on muscle protein synthesis

2019 ◽  
Vol 49 (6) ◽  
pp. 1275-1286
Author(s):  
Milena Casagranda ◽  
Priscila Berti Zanella ◽  
Alexandra Ferreira Vieira ◽  
Rodrigo Cauduro Oliveira Macedo
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Protein Biosynthesis ◽  
Nitrogen Compound ◽  
Milk Proteins ◽  
Control Group ◽  
Content Type ◽  
Protein Sources

Purpose The purpose of the study was to evaluate the acute effect of milk proteins supplementation, compared to another nitrogen compound on muscle protein synthesis. Design/methodology/approach The search was conducted on MEDLINE® (via PUBMED®), Cochrane and Embase databases, using the terms “whey proteins,” “caseins,” “milk proteins,” “protein biosynthesis,” “human” and its related entry terms. The selected outcome was fractional synthetic rate (FSR) before (0) and 3 h after consumption of milk proteins, compared to supplementation with other protein sources or isolated amino acids. Findings The results were expressed as mean difference (MD) of absolute values between treatments with confidence interval (CI) of 95 per cent. Of the 1,913 identified studies, 4 were included, with a total of 74 participants. Milk proteins generated a greater FSR (MD 0.03 per cent/h, CI 95 per cent 0.02-0.04; p < 0.00001), compared to control group. Acute consumption of milk proteins promotes higher increase in FSR than other protein sources or isolated amino acids. Originality/value This paper is a systematic review of the effects of milk proteins supplementation, which is considered an important subject because of its large consumption among athletes and physical exercise practitioners.

scholarly journals Rapid Microarray-Based Identification of DifferentmecAAlleles in Staphylococci

2012 ◽  
Vol 56 (11) ◽  
pp. 5547-5554 ◽  
Author(s):  
Stefan Monecke ◽  
Elke Müller ◽  
Stefan Schwarz ◽  
Helmut Hotzel ◽  
Ralf Ehricht
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid Sequences ◽  
Animal Origin ◽  
Coagulase Negative Staphylococci ◽  
Content Type ◽  
Diagnostic Assays ◽  
Rapid Discrimination ◽  
Unique Amino Acid ◽  
Full Length Gene

ABSTRACTTo screen isolates and to identifymecAalleles, publishedmecAsequences were analyzed, and a microarray for the rapid discrimination ofmecAalleles was designed. A GenBank analysis yielded 135 full-length gene sequences annotated asmecA. These sequences clustered into 32 different alleles corresponding to 28 unique amino acid sequences and to 15 distinct hybridization patterns on this microarray. A collection of 78 clinical and veterinary isolates ofStaphylococcusspp. was characterized using this assay. Nine of the 15 expected patterns, as well as one as-yet-unknown pattern, were identified. These patterns were detected in various epidemic methicillin-resistantStaphylococcus aureusstrains, inS. pseudintermedius, and in coagulase-negative species such asS. epidermidis,S. fleurettii, orS. haemolyticus. There was no correlation between the differentmecAhybridization patterns and the SCCmectype. Determination of MICs showed thatmecAalleles corresponding to only four of these nine patterns were associated with β-lactam resistance. ThemecAalleles that did not confer β-lactam resistance were largely restricted to coagulase-negative staphylococci of animal origin, such asS. sciuriandS. vitulinus. Because of the diversity of sequences and the different impact on β-lactam susceptibility, the existence of differentmecAalleles needs to be taken into account when designing diagnostic assays for the detection ofmecA.

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