scholarly journals Genome Survey and Characterization of Endophytic Bacteria Exhibiting a Beneficial Effect on Growth and Development of Poplar Trees

2008 ◽  
Vol 75 (3) ◽  
pp. 748-757 ◽  
Author(s):  
Safiyh Taghavi ◽  
Craig Garafola ◽  
S�bastien Monchy ◽  
Lee Newman ◽  
Adam Hoffman ◽  
...  
Keyword(s):  
Plant Growth ◽  
Beneficial Effect ◽  
Biomass Production ◽  
Growth And Development ◽  
Endophytic Bacteria ◽  
Sequence Data ◽  
Growth Promotion ◽  
Poplar Trees ◽  
Sequestration Potential ◽  
Plant Hosts

ABSTRACT The association of endophytic bacteria with their plant hosts has a beneficial effect for many different plant species. Our goal is to identify endophytic bacteria that improve the biomass production and the carbon sequestration potential of poplar trees (Populus spp.) when grown in marginal soil and to gain an insight in the mechanisms underlying plant growth promotion. Members of the Gammaproteobacteria dominated a collection of 78 bacterial endophytes isolated from poplar and willow trees. As representatives for the dominant genera of endophytic gammaproteobacteria, we selected Enterobacter sp. strain 638, Stenotrophomonas maltophilia R551-3, Pseudomonas putida W619, and Serratia proteamaculans 568 for genome sequencing and analysis of their plant growth-promoting effects, including root development. Derivatives of these endophytes, labeled with gfp, were also used to study the colonization of their poplar hosts. In greenhouse studies, poplar cuttings (Populus deltoides � Populus nigra DN-34) inoculated with Enterobacter sp. strain 638 repeatedly showed the highest increase in biomass production compared to cuttings of noninoculated control plants. Sequence data combined with the analysis of their metabolic properties resulted in the identification of many putative mechanisms, including carbon source utilization, that help these endophytes to thrive within a plant environment and to potentially affect the growth and development of their plant hosts. Understanding the interactions between endophytic bacteria and their host plants should ultimately result in the design of strategies for improved poplar biomass production on marginal soils as a feedstock for biofuels.

Metabolome adjustments in ectomycorrhizal Populus × canescens associated with strong promotion of plant growth by Paxillus involutus despite a very low root colonization rate

2020 ◽  
Vol 40 (12) ◽  
pp. 1726-1743
Author(s):  
Agnieszka Szuba ◽  
Łukasz Marczak ◽  
Izabela Ratajczak
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Growth Promotion ◽  
P Uptake ◽  
Soluble Carbohydrates ◽  
Paxillus Involutus ◽  
Root Tips ◽  
Poplar Trees ◽  
Related Proteins ◽  
Poplar Growth

Abstract It is believed that resource exchange, which is responsible for intensified growth of ectomycorrhizal plants, occurs in the fungus–plant interface. However, increasing evidence indicates that such intensified plant growth, especially root growth promotion, may be independent of root colonization. Nevertheless, the molecular adjustments in low-colonized plants remain poorly understood. Here, we analysed the metabolome of Populus × canescens microcuttings characterized by significantly increased growth triggered by inoculation with Paxillus involutus, which successfully colonized only 2.1 ± 0.3% of root tips. High-throughput metabolomic analyses of leaves, stems and roots of Populus × canescens microcuttings supplemented with leaf proteome data were performed to determine ectomycorrhiza-triggered changes in N-, P- and C-compounds. The molecular adjustments were relatively low in low-colonized (M) plants. Nevertheless, the levels of foliar phenolic compounds were significantly increased in M plants. Increases of total soluble carbohydrates, starch as well as P concentrations were also observed in M leaves along with the increased abundance of the majority of glycerophosphocholines detected in M roots. However, compared with the leaves of the non-inoculated controls, M leaves presented lower concentrations of both N and most photosynthesis-related proteins and all individual mono- and disaccharides. In M stems, only a few compounds with different abundances were detected, including a decrease in carbohydrates, which was also detected in M roots. Thus, these results suggest that the growth improvement of low-colonized poplar trees is independent of an increased photosynthesis rate, massively increased resource (C:N) exchange and delivery of most nutrients to leaves. The mechanism responsible for poplar growth promotion remains unknown but may be related to increased P uptake, subtle leaf pigment changes, the abundance of certain photosynthetic proteins, slight increases in stem and root amino acid levels and the increase in flavonoids (increasing the antioxidant capacity in poplar), all of which improve the fitness of low-colonized poplars.

Interactions between putatively endophytic bacteria and tall fescue (Festuca arundinacea): plant growth promotion and colonization in host and non-host cultivars

2019 ◽  
Vol 451 (1-2) ◽  
pp. 207-220
Author(s):  
Patricia Vaz Jauri ◽  
Cecilia Taulé ◽  
Maria Cecilia de los Santos ◽  
Belén Fernandez ◽  
Andrés Di Paolo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Endophytic Bacteria ◽  
Growth Promotion

scholarly journals Characterization of bacterial endophytes from the roots of native and cultivated Brazil nut trees (Bertholletia excelsa)

2019 ◽  
Vol 49 (4) ◽  
pp. 257-267
Author(s):  
Patrícia Bombonati CHALITA ◽  
Eliane do Nascimento Cunha FARIAS ◽  
Ismaele Breckenfeld da COSTA ◽  
Brenda Ferreira SOUSA ◽  
Marco Antônio Oliveira dos SANTOS ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Nifh Gene ◽  
Aluminum Phosphate ◽  
Brazil Nut ◽  
Indole Compounds ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Brazil nut is a very important nontimber forest product in the Amazon region. Propagation of this tree still represents a challenge due to slow and uneven seed germination. In this context, plant growth-promoting bacteria can facilitate the process of propagation. The aims of this study were to isolate and characterize endophytic bacteria from the roots of Brazil nut trees in native terra firme forest and cultivation areas in northern Brazil, and to identify mechanisms by which bacteria act in plant growth promotion. Overall, 90 bacterial isolates were obtained from the roots of Brazil nut trees in monoculture, agroforestry and native forest areas by using different semisolid media. The isolates were characterized by sequencing the 16S rRNA gene. Plant growth-promoting characteristics were evaluated by the presence of the nifH gene, aluminum phosphate solubilization and the production of indole compounds. The isolates were affiliated with 18 genera belonging to 5 different classes (α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Bacilli and Actinobacteria). The genus Bacillus was predominant in the forest and monoculture areas. Fourteen isolates presented the nifH gene. Most of the bacteria were able to solubilize aluminum phosphate and synthetize indole compounds. The results indicated high diversity of endophytic bacteria present among the roots of Brazil nut trees, mainly in the agroforestry area, which could be related to soil attributes. Among the 90 isolates, the 22 that presented the best results regarding plant growth promotion traits were good candidates for testing in seedling production of Brazil nut trees.

scholarly journals Seed Endophytic Bacteria of Pearl Millet (Pennisetum glaucum L.) Promote Seedling Development and Defend Against a Fungal Phytopathogen

2021 ◽  
Vol 12 ◽  
Author(s):  
Kanchan Kumar ◽  
Anand Verma ◽  
Gaurav Pal ◽  
Anubha ◽  
James F. White ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Fluorescence Microscopy ◽  
Pearl Millet ◽  
Growth And Development ◽  
Endophytic Bacteria ◽  
Pennisetum Glaucum ◽  
Structural Deformation ◽  
Pantoea Stewartii

Seed endophytic bacteria (SEB) are primary symbionts that play crucial roles in plant growth and development. The present study reports the isolation of seven culturable SEB including Kosakonia cowanii (KAS1), Bacillus subtilis (KAS2), Bacillus tequilensis (KAS3), Pantoea stewartii (KAS4), Paenibacillus dendritiformis (KAS5), Pseudomonas aeruginosa (KAS6), and Bacillus velezensis (KAS7) in pearl millet seeds. All the isolates were characterized for their plant growth promoting activities. Most of the SEB also inhibited the growth of tested fungal phytopathogens in dual plate culture. Removal of these SEB from seeds compromised the growth and development of seedlings, however, re-inoculation with the SEB (Kosakonia cowanii, Pantoea stewartii, and Pseudomonas aeruginosa) restored the growth and development of seedlings significantly. Fluorescence microscopy showed inter and intracellular colonization of SEB in root parenchyma and root hair cells. Lipopeptides were extracted from all three Bacillus spp. which showed strong antifungal activity against tested fungal pathogens. Antifungal lipopeptide genes were also screened in Bacillus spp. After lipopeptide treatment, live-dead staining with fluorescence microscopy along with bright-field and scanning electron microscopy (SEM) revealed structural deformation and cell death in Fusarium mycelia and spores. Furthermore, the development of pores in the membrane and leakages of protoplasmic substances from cells and ultimately death of hyphae and spores were also confirmed. In microcosm assays, treatment of seeds with Bacillus subtilis or application of its lipopeptide alone significantly protected seedlings from Fusarium sp. infection.

scholarly journals Soil Type-Dependent Interactions of P-Solubilizing Microorganisms with Organic and Inorganic Fertilizers Mediate Plant Growth Promotion in Tomato

Agronomy ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 213 ◽  
Author(s):  
Isaac Mpanga ◽  
Harrison Dapaah ◽  
Joerg Geistlinger ◽  
Uwe Ludewig ◽  
Günter Neumann
Keyword(s):  
Plant Growth ◽  
Biomass Production ◽  
Plant Growth Promotion ◽  
Soil Type ◽  
Growth Promotion ◽  
Inorganic Fertilizers ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Organic And Inorganic Fertilizers ◽  
Npk Fertilization

The use of plant growth-promoting microorganisms (PGPMs) as bio-effectors (BEs) to improve the nutrient acquisition of crops has a long history. However, limited reproducibility of the expected effects still remains a major challenge for practical applications. Based on the hypothesis that the expression of PGPM effects depends on soil type and the properties of the applied fertilizers, in this study, the performance of selected microbial inoculants was investigated for two contrasting low-fertility soils supplied with different organic and inorganic fertilizers. Greenhouse experiments were conducted with tomato on an alkaline sandy loam of pH 7.8 and an acidic loamy sand of pH 5.6 with limited phosphate (P) availability. Municipal waste compost, with and without poultry manure (PM), rock phosphate (RP), stabilized ammonium, and mineral nitrogen, phosphorus and potassium (NPK) fertilization were tested as fertilizer variants. Selected strains of Bacillus amyloliquefaciens (Priest et al. 1987) Borriss et al. 2011 (FZB42) and Trichoderma harzianum Rifai (OMG16) with proven plant growth-promoting potential were used as inoculants. On both soils, P was identified as a major limiting nutrient. Microbial inoculation selectively increased the P utilization in the PM-compost variants by 116% and 56% on the alkaline and acidic soil, while RP utilization was increased by 24%. This was associated with significantly increased shoot biomass production by 37–42%. Plant growth promotion coincided with a corresponding stimulation of root growth, suggesting improved spatial acquisition of soluble soil P fractions, associated also with improved acquisition of nitrogen (N), potassium (K), magnesium (Mg), and calcium (Ca). There was no indication for mobilization of sparingly soluble Ca phosphates via rhizosphere acidification on the alkaline soil, and only mineral NPK fertilization reached a sufficient P status and maximum biomass production. However, on the moderately acidic soil, FZB42 significantly stimulated plant growth of the variants supplied with Ca–P in the form of RP + stabilized ammonium and PM compost, which was equivalent to NPK fertilization; however, the P nutritional status was sufficient only in the RP and NPK variants. The results suggest that successful application of microbial biofertilizers requires more targeted application strategies, considering the soil properties and compatible fertilizer combinations.

scholarly journals Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion

2008 ◽  
Vol 25 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Armando C. F. Dias ◽  
Francisco E. C. Costa ◽  
Fernando D. Andreote ◽  
Paulo T. Lacava ◽  
Manoel A. Teixeira ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Endophytic Bacteria ◽  
Growth Promotion

Identification of Endophytic Bacteria in Panax ginseng Seeds and Their Potential for Plant Growth Promotion

2014 ◽  
Vol 22 (4) ◽  
pp. 306-312 ◽  
Author(s):  
Yurry Um ◽  
Bo Ra Kim ◽  
Jin Ju Jeong ◽  
Chan Moon Chung ◽  
Yi Lee
Keyword(s):  
Plant Growth ◽  
Panax Ginseng ◽  
Plant Growth Promotion ◽  
Endophytic Bacteria ◽  
Growth Promotion

Potentially Diazotrophic Endophytic Bacteria Associated to Sugarcane are Effective in Plant Growth-promotion

2018 ◽  
Vol 21 (3) ◽  
pp. 1-15 ◽  
Author(s):  
Maria Leite ◽  
Arthur Pereira ◽  
Adijailton Souza ◽  
Pedro Andrade ◽  
Marisângela Barbosa ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Diazotrophic Endophytic Bacteria
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