scholarly journals Molecular Tracking, through Processing, of Campylobacter Strains Colonizing Broiler Flocks

2011 ◽  
Vol 77 (16) ◽  
pp. 5722-5729 ◽  
Author(s):  
Karen T. Elvers ◽  
Victoria K. Morris ◽  
Diane G. Newell ◽  
Vivien M. Allen

ABSTRACTMany of the poultry flocks produced in the United Kingdom are colonized withCampylobacter, and the intensive nature of poultry processing usually results in contaminated carcasses. In this study, a previously reported molecular oligonucleotide probe method was used to track a specific flock-colonizing strain(s) on broiler carcasses during processing in two United Kingdom commercial poultry processing plants. FiveCampylobacter-positive flocks were sampled at four points along the processing line, postbleed, postpluck, prechill, and postchill, and twoCampylobacter-negative flocks processed immediately after positive flocks were sampled prechill.flaAwas sequenced fromCampylobacterstrains isolated from these flocks, and strain-specific probes were synthesized. Skin and cecal samples were plated onto selective agar to give individual colonies, which were transferred onto membranes. These were then hybridized with the strain- and genus-specific probes. For all the 5 positive flocks, there was a significant reduction in campylobacters postbleed compared to postpluck but no subsequent fall on sampling pre- and postchill, and the strain(s) predominating on the carcasses throughout processing came from the flock being processed. This indicates that strains from the abattoir environment were not a significant cause of carcass contamination in flocks with well-established campylobacter colonization. However, negative flocks that were preceded by positive flocks were contaminated by strains that did not generally originate from the predominating strains recovered from the ceca of the previous positive flocks. This suggests that the abattoir environment has a significant role in the contamination of carcasses from negative but not fully colonized flocks.

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Agnese Rondoni ◽  
Elena Millan ◽  
Daniele Asioli

PurposePlant-based eggs have recently been developed to provide consumers with a healthier, animal-friendlier and more sustainable alternative to conventional eggs. The purpose of this paper is to investigate intrinsic and extrinsic attribute preferences for three prototypes of plant-based egg, namely the liquid, powder and egg-shaped.Design/methodology/approachNine focus groups in the United Kingdom and nine in Italy were conducted, with a total of 180 participants. A thematic analysis of results was conducted.FindingsIn terms of intrinsic product attributes, consumers' preferences for colour, shape, taste, ingredients, nutrients, method of production and shelf-life for plant-based eggs were revealed. Regarding the extrinsic attributes, preferences for price, packaging, country of origin and product naming emerged. Similarities and differences between consumers from the two countries are also discussed. Differences in preferences also emerged between vegan and non-vegan consumers.Research limitations/implicationsThis study adds to the existing knowledge on consumers' preferences for new plant-based food alternatives and identifies future quantitative approaches based on qualitative findings.Practical implicationsResults from this study can assist plant-based egg manufacturers in improving their products in line with consumers' expectations, which may help reducing risk of product failure.Originality/valueThis study is the first to investigate consumers' preferences, expectations and needs for new food products like plant-based eggs and provides information that can be practically applied by manufacturers, as well as suggestions for future research.


2018 ◽  
Vol 84 (14) ◽  
Author(s):  
Lydia J. Leon ◽  
Ronan Doyle ◽  
Ernest Diez-Benavente ◽  
Taane G. Clark ◽  
Nigel Klein ◽  
...  

ABSTRACT In this study, differences in the placental microbiota from term and preterm deliveries in a large pregnancy cohort in the United Kingdom were studied by using 16S-targeted amplicon sequencing. The impacts of contamination from DNA extraction, PCR reagents, and the delivery itself were also examined. A total of 400 placental samples from 256 singleton pregnancies were analyzed, and differences between spontaneous preterm-, nonspontaneous preterm-, and term-delivered placentas were investigated. DNA from recently delivered placentas was extracted, and screening for bacterial DNA was carried out by using targeted sequencing of the 16S rRNA gene on the Illumina MiSeq platform. Sequenced reads were analyzed for the presence of contaminating operational taxonomic units (OTUs) identified via sequencing of negative extraction and PCR-blank samples. Differential abundances and between-sample (beta) diversity metrics were then compared. A large proportion of the reads sequenced from the extracted placental samples mapped to OTUs that were also found for negative extractions. Striking differences in the compositions of samples were also observed, according to whether the placenta was delivered abdominally or vaginally, providing strong circumstantial evidence for delivery contamination as an important contributor to observed microbial profiles. When OTU- and genus-level abundances were compared between the groups of interest, a number of organisms were enriched in the spontaneous preterm-delivery cohort, including organisms that have been associated previously with adverse pregnancy outcomes, specifically Mycoplasma spp. and Ureaplasma spp. However, analyses of the overall community structure did not reveal convincing evidence for the existence of a reproducible “preterm placental microbiome.” IMPORTANCE Preterm birth is associated with both psychological and physical disabilities and is the leading cause of infant morbidity and mortality worldwide. Infection is known to be an important cause of spontaneous preterm birth, and recent research has implicated variation in the “placental microbiome” in the risk of preterm birth. Consistent with data from previous studies, the abundances of certain clinically relevant species differed between spontaneous preterm- and nonspontaneous preterm- or term-delivered placentas. These results support the view that a proportion of spontaneous preterm births have an intrauterine-infection component. However, an additional observation from this study was that a substantial proportion of sequenced reads were contaminating reads rather than DNA from endogenous, clinically relevant species. This observation warrants caution in the interpretation of sequencing outputs from low-biomass samples such as the placenta.


Significance The United Kingdom eyes a strategic tilt to the Indo-Pacific, with South-east Asia a key focus because of its economic dynamism and the convening power of ASEAN. The aircraft carrier HMS Queen Elizabeth will deploy to the Indo-Pacific for six months later this year. Impacts The United Kingdom’s view of China as a systemic challenger makes a trade pact between the two countries unlikely. UK efforts to conclude a trade deal with India will be frustrated by Delhi’s protectionist approach. London will pursue closer defence cooperation with Tokyo.


Significance All three countries are united by the objective to mitigate US-China rivalry by promoting multilateralism and engagement among middle powers, while at the same time using these to contain China’s behaviour and ambitions in the region. Impacts Engaging with like-minded middle powers in the Indo-Pacific will help Europe distance itself from Washington’s tougher policy on China. The Indo-Pacific vision will result in deepening defence partnerships with India, plus Australia and Japan, such as through arms sales. The Indo-Pacific will be an area of potential future collaboration and cooperation between the United Kingdom and EU countries.


Significance However, there has been a notable change in the EU’s tone. In July, the European Commission unexpectedly paused legal action against the United Kingdom for an alleged breach of the NIP, and when London announced on September 6 that it was suspending key elements indefinitely, the EU’s response was muted. Impacts France is so deeply aggrieved over AUKUS that any further UK breaches of the Withdrawal Agreement could prompt a bad-tempered response. The possibility of an early assembly election in Northern Ireland would complicate EU-UK attempts to resolve the NIP issue. The exclusion of high profile, pro-EU politicians in the UK cabinet reshuffle shows how important the Brexit agenda remains for London.


2020 ◽  
Vol 22 (3) ◽  
pp. 233-245
Author(s):  
Yasmine Dominguez-Whitehead ◽  
Felix Maringe

PurposeThis paper provides a cross-national analysis of PhD supervision models, milestones and examination procedures in order to compare PhD programs and their practices.Design/methodology/approachA comparative approach is employed, which systematically interrogates PhD supervision models, milestones and examination procedures in the United Kingdom, South Africa and the United States via a comprehensive review of the practices and literature.FindingsThe findings indicate the ramifications of the different approaches and highlight the benefits and drawbacks associated with the different models.Originality/valueBy making explicit the dominant supervision models, milestones and examination procedures that exist in the United Kingdom, South Africa and the United States, the authors shed light on the somewhat obscure path to earning a PhD degree.


2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Emma Beacom ◽  
Sinéad Furey ◽  
Lynsey Elizabeth Hollywood ◽  
Paul Humphreys

PurposeA number of food poverty definitions have been identified by academics and various government organisations globally; however, there exists no government-endorsed definition of food poverty in the United Kingdom (UK), and there remains a gap regarding how relevant current food poverty definitions are in the Northern Ireland (NI)/UK contexts.Design/methodology/approachInterviews (n = 19) with a range of stakeholders (e.g. policymakers, politicians, community advice centre workers, consumer sub-group representatives, food bank and food redistribution organisation representatives) were conducted to examine (1) the usefulness of a food poverty definition, (2) what a food poverty definition should include and (3) the applicability of an existing definition (Radimer et al., 1992) in the NI/UK context. Data was thematically analysed using QSR NVivo (v.12).FindingsDefinition was considered important to increase awareness and understanding. Any consideration of revising the Radimer et al. (1992) definition, or of establishing a new standardised definition, should seek to reduce/remove ambiguity and subjectivity of terminology used (i.e. more clearly defining what the terms “adequate”, “sufficient”, “quality” and “socially acceptable ways” mean in this context).Practical implicationsThis research emphasises the importance of appropriately conceptually defining social phenomena such as food poverty, as a first step to constructing and reviewing measurement approaches and ultimately assessing predictors and recommending solutions.Originality/valueThis research addresses the gap relating to stakeholders’ opinion on food poverty definition and contributes recommendations for modifying the Radimer et al. (1992) definition in the NI/UK and present-day contexts.


2020 ◽  
Vol 33 (6) ◽  
pp. 1219-1246 ◽  
Author(s):  
Bikram Chatterjee ◽  
Carolyn J. Cordery ◽  
Ivo De Loo ◽  
Hugo Letiche

PurposeIn this paper, we concentrate on the use of research assessment (RA) systems in universities in New Zealand (NZ) and the United Kingdom (UK). Primarily we focus on PBRF and REF, and explore differences between these systems on individual and systemic levels. We ask, these days, in what way(s) the systemic differences between PBRF and REF actually make a difference on how the two RA systems are experienced by academic staff.Design/methodology/approachThis research is exploratory and draws on 19 interviews in which accounting researchers from both countries offer reflections on their careers and how RA (systems) have influenced these careers. The stories they tell are classified by regarding RA in universities as a manifestation of the spectacle society, following Debord (1992) and Flyverbom and Reinecke (2017).FindingsBoth UK and New Zealand academics concur that their research activities and views on research are very much shaped by journal rankings and citations. Among UK academics, there seems to be a greater critical attitude towards the benefits and drawbacks of REF, which may be related to the history of REF in their country. Relatively speaking, in New Zealand, individualism seems to have grown after the introduction of the PBRF, with little active pushback against the system. Cultural aspects may partially explain this outcome. Academics in both countries lament the lack of focus on practitioner issues that the increased significance of RA seems to have evoked.Research limitations/implicationsThis research is context-specific and may have limited applicability to other situations, academics or countries.Practical implicationsRA and RA systems seem to be here to stay. However, as academics we can, and ought to, take responsibility to try to ensure that these systems reflect the future of accounting (research) we wish to create. It is certainly not mainly or solely up to upper management officials to set this in motion, as has occasionally been claimed in previous literature. Some of the academics who participated in this research actively sought to bring about a different future.Originality/valueThis research provides a unique contextual analysis of accounting academics' perspectives and reactions to RA and RA systems and the impact these have had on their careers across two countries. In addition, the paper offers valuable critical reflections on the application of Debord's (1992) notion of the spectacle society in future accounting studies. We find more mixed and nuanced views on RA in academia than many previous studies have shown.


2016 ◽  
Vol 42 (4) ◽  
pp. 390-410 ◽  
Author(s):  
Richard A. Bernardi ◽  
Taylor L. Delande ◽  
Kimberly A. Zamojcin

Purpose – The purpose of this paper is to examine the trends in accounting-education publications and the influence of journal rankings for authors from Australia, Canada, New Zealand and the UK. Design/methodology/approach – The authors included the publications in ten accounting-education journals for the 20-year period from 1993 to 2012. Findings – The data provide insights into the perceptions of accounting-education journals by authors from four countries. The authors found that, while the use of Accounting Education as a publication outlet for accounting authors from Australia and the UK was relatively stable, the use of Accounting Education as a publication outlet increased (decreased) for the accounting authors from New Zealand (Canada). The authors also found that, while coauthoring by the accounting authors from Australia and the UK increased slightly, coauthoring by the accounting authors from Canada and New Zealand increased during the 20-year period. Research limitations/implications – The data suggests a tendency by the authors from these four countries to publish their accounting-education research in journals that had been ranked as a top accounting journal. Originality/value – This paper is the first paper to consider trends in international accounting-education publications. The data in this research can be used by accounting faculty wishing to assess which journals their colleagues publish in most frequently.


Significance The budget will prove to be all but the last political event before the final campaign and the May 7 general election. Although the detail had to be determined in cooperation with the Liberal Democrats, Osborne's package was an unashamed pitch for a Conservative term of office. The crucial contest at the election will be the government's claim to have delivered on economic recovery and deficit reduction over the past five years, versus the Labour Party's assertion that its own preferred route to budgetary stability is better balanced and more socially acceptable. Impacts The link, if any, between the economy and politics is uncertain: the Conservatives won in 1992 (recession) but lost in 1997 (boom). The United Kingdom has experienced a 'voteless recovery' -- a huge move from pessimism to optimism, with no benefit for the Conservatives. Benign international economic conditions (notably the sharp fall in global oil prices) have boosted growth for 2015. This shift could be easily reversed if oil prices were to return to 2012-14 levels. Assumptions that the 'age of austerity' is over and the budget will achieve balance in the next parliament are very premature.


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