scholarly journals Constitutive Expression of the Proteorhodopsin Gene by a Flavobacterium Strain Representative of the Proteorhodopsin-Producing Microbial Community in the North Sea

2010 ◽  
Vol 76 (10) ◽  
pp. 3187-3197 ◽  
Author(s):  
Thomas Riedel ◽  
Jürgen Tomasch ◽  
Ina Buchholz ◽  
Jenny Jacobs ◽  
Mario Kollenberg ◽  
...  
Keyword(s):  
Water Sample ◽  
North Sea ◽  
Sea Water ◽  
Constitutive Expression ◽  
Physiological Role ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Degenerate Primers ◽  
The North ◽  
The North Sea

ABSTRACT Proteorhodopsin (PR), a photoactive proton pump containing retinal, is present in approximately half of all bacteria in the ocean, but its physiological role is still unclear, since very few strains carrying the PR gene have been cultured. The aim of this work was to characterize PR diversity in a North Sea water sample, cultivate a strain representative of North Sea PR clusters, and study the effects of light and carbon concentration on the expression of the PR gene. A total of 117 PR sequences, of which 101 were unique, were obtained from a clone library of PCR-amplified PR gene fragments. Of the North Sea PRs, 97% were green light absorbing, as inferred from the amino acid at position 105; 67% of the PR protein fragments showed closest similarity to PRs from Alphaproteobacteria, 4% showed closest similarity to PRs from Gammaproteobacteria, and 29% showed closest similarity to PRs from “Bacteroidetes”/Flavobacteria. The dominant PR cluster (comprising 18% of all PRs) showed a high degree of similarity to the PR from the cultivated Roseobacter strain HTCC2255. The relative abundances of the North Sea PR clusters were confirmed by quantitative PCR. They were detected in metagenomic fragments from coastal oceans worldwide with various degrees of abundance. Several hundred bacterial strains from the North Sea water sample were cultivated on oligocarbophilic media. By screening with degenerate primers, two strains carrying the PR gene were identified. Their 16S rRNA gene sequences were identical and affiliated with a Bacteroidetes subcluster from the North Sea. The PR sequence of isolate PRO95 was completed by chromosomal walking. It was 76% identical to that of Dokdonia donghaensis MED134 and was functional, as indicated by the signature amino acids. PRO95 expressed its PR gene in liquid media containing between 9.7 and 121 mM carbon, both in the light and in the dark. Growth was not enhanced by light. Thus, the detection of the physiological role of PR may require more sensitive methods.

scholarly journals Phylogeny and Synonymy of Gyrodinium heterostriatum comb. nov. (Dinophyceae), a Common Unarmored Dinoflagellate in the World Oceans [plus supplementary materials]

2020 ◽  
Vol 59 (2) ◽  
pp. 77-87
Author(s):  
Fernando Gómez ◽  
Luis F. Artigas ◽  
Rebecca J. Gast
Keyword(s):  
North Sea ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Plasticity ◽  
Sister Group ◽  
Rrna Gene ◽  
Microbial World ◽  
The North ◽  
The North Sea ◽  
Environmental Sequences

The North Sea and the English Channel are regions with a long tradition of plankton studies, where the colony-forming haptophyte Phaeocystis globosa dominates the spring phytoplankton blooms. Among its predators, we investigated an abundant unarmored dinoflagellate (~3000 cells per liter) in the North Sea in May 2019. It has been reported in the literature as Gymnodinium heterostriatum or G. striatissimum, and often identified as Gyrodinium spirale. Phylogenetic analyses using the small-, large subunit- and Internal Transcriber Spacers of the ribosomal RNA (SSU-, LSU-, ITS rRNA) gene sequences indicate that our isolates clustered within the Gyrodinium clade. The new sequences formed a sister group with sequences of the freshwater taxon Gyrodinium helveticum, being one of the infrequent marine-freshwater transitions in the microbial world. This isolate is the first characterized member of a clade of numerous environmental sequences widely distributed from cold to tropical seas. This common and abundant taxon has received several names due to its morphological plasticity (changes of size and shape, often deformed after engulfing prey) and the difficulty in discerning surface striation. We conclude that the priority is for the species name Gymnodinium heterostriatum Kofoid & Swezy 1921, a new name that was proposed for Gymnodinium spirale var. obtusum sensu Dogiel 1906. The species Gyrodinium striatissimum (Hulburt 1957) Gert Hansen & Moestrup 2000 and Gymnodinium lucidum D. Ballantine in Parke & Dixon 1964 (=G. hyalinum M. Lebour 1925) are posterior synonyms. We propose Gyrodinium heterostriatum comb. nov. for Gymnodinium heterostriatum.

scholarly journals An ensemble study of extreme storm surge related water levels in the North Sea in a changing climate

Ocean Science ◽  
2009 ◽  
Vol 5 (3) ◽  
pp. 369-378 ◽  
Author(s):  
A. Sterl ◽  
H. van den Brink ◽  
H. de Vries ◽  
R. Haarsma ◽  
E. van Meijgaard
Keyword(s):  
North Sea ◽  
Climate Model ◽  
Sea Water ◽  
Global Climate ◽  
Global Climate Model ◽  
Storm Surges ◽  
Water Levels ◽  
The North ◽  
Extreme Storm ◽  
The North Sea

Abstract. The height of storm surges is extremely important for a low-lying country like The Netherlands. By law, part of the coastal defence system has to withstand a water level that on average occurs only once every 10 000 years. The question then arises whether and how climate change affects the heights of extreme storm surges. Published research points to only small changes. However, due to the limited amount of data available results are usually limited to relatively frequent extremes like the annual 99%-ile. We here report on results from a 17-member ensemble of North Sea water levels spaning the period 1950–2100. It was created by forcing a surge model of the North Sea with meteorological output from a state-of-the-art global climate model which has been driven by greenhouse gas emissions following the SRES A1b scenario. The large ensemble size enables us to calculate 10 000 year return water levels with a low statistical uncertainty. In the one model used in this study, we find no statistically significant change in the 10 000 year return values of surge heights along the Dutch during the 21st century. Also a higher sea level resulting from global warming does not impact the height of the storm surges. As a side effect of our simulations we also obtain results on the interplay between surge and tide.

Pollution of the Outer Oslofjord

1991 ◽  
Vol 24 (10) ◽  
pp. 321-322
Author(s):  
Kjell Baalsrud
Keyword(s):  
North Sea ◽  
General Circulation ◽  
Sea Water ◽  
Sewage Treatment ◽  
Circulation Pattern ◽  
Polluted Water ◽  
The North ◽  
Recreation Area ◽  
The North Sea ◽  
Sill Depth

The Outer Oslofjord has recently been subject to concern. 1. The Inner Oslofjord. Covers an area of 193 km2, is 160 m deep and is separated from the Outer Oslofjord by the narrow Drøbak sound with a sill depth of less than 20 metres. This part of the Oslofjord constitutes an enclosed body of sea water, sensitive to pollution, receiving sewage from approximately 650,000 inhabitants. In spite of modern sewage treatment, the fjord still suffers torn eutrophication problems resulting in reduced oxygen in the deep water, and areas of anoxic bottom water. The fjord is an important recreation area. 2. The Outer Oslofjord. Recent findings indicate that eutrophication is slowly increasing. An increasing eutrophication of the Outer Oslofjord wil also increase the need (and cost) of better sewage treatment in the Inner Oslofjord. 3. The North Sea. The quality of the water in the Oslofjord area is also dependent on the water it receives from the Skagerrak. The Skagerrak water will periodically receive polluted water from the southern North Sea and Kattegat. When these episodes coincide with water renewals between the Oslofjord and the Skagerrak, the fjord will receive polluted water from other countries. The Oslofjord water will in turn discharge into the Skagerrak, but due to the general circulation pattern, this will mainly influence the Norwegian south-east coast.

scholarly journals Seasonal variations in the occurrence of Vibriovulnificusalong the Dutch coast

1994 ◽  
Vol 112 (2) ◽  
pp. 285-290 ◽  
Author(s):  
J. Veenstra ◽  
P. J. G. M. Rietra ◽  
J. M. Coster ◽  
E. Slaats ◽  
S. Dirks-Go
Keyword(s):  
Seasonal Variation ◽  
Water Temperature ◽  
North Sea ◽  
Seasonal Variations ◽  
Water Samples ◽  
Human Disease ◽  
Sea Water ◽  
Dutch Coast ◽  
The North ◽  
The North Sea

SUMMARYThe seasonal variation in the occurrence ofV. vulnificusin relation to water temperature and salinity was studied along the Dutch coast. In two consecutive yearsV. vulnificusstrains could be isolated in August when the water temperature was highest. The indole-positive strains isolated from North Sea water samples were identical to most strains isolated from human disease and from the environment. However, strains isolated from four of five patients living in countries around the North Sea were different from the North Sea isolates in that they were indole-negative and have a lower NaCl tolerance.

Annual and spatial variability in endo- and ectoparasite infections of North Sea cod (Gadus morhua Linnaeus, 1758) larvae, post-larvae and juveniles

2014 ◽  
Vol 59 (2) ◽  
Author(s):  
Foojan Mehrdana ◽  
Qusay Bahlool ◽  
Alf Skovgaard ◽  
Jesper Kuhn ◽  
Per Kania ◽  
...  
Keyword(s):  
Spatial Variability ◽  
North Sea ◽  
Gadus Morhua ◽  
Sea Water ◽  
Atlantic Cod ◽  
The North ◽  
Infection Pressure ◽  
High Prevalence ◽  
Atlantic Cod Larvae ◽  
The North Sea

AbstractA parasitological investigation was performed on a total of 5380 Atlantic cod larvae, post-larvae and small juveniles sampled from the North Sea during a period of five years. The copepod Caligus elongatus (Von Nordmann, 1832) and the nematode Hysterothylacium aduncum (Rudolphi, 1802) were found at a relatively high prevalence of infection (4.6% and 5.2%, respectively). The infection by both parasites showed annual and spatial variability. C. elongatus showed a higher prevalence in 1992 compared to the following years, whereas the prevalence of H. aduncum increased from 1992 to 2001.We observed a relation between parasite distribution and parameters such as latitude and water depth. Adult digeneans (Lecithaster gibbosus and Derogenes varicus) and larval cestodes were also found with lower infection rates. Since changes of infection levels coincided with increasing North Sea water temperature in the studied period, it is hypothesized that temperature may affect parasite population levels. However, it is likely that other environmental factors may contribute to the observed variations. Absence of infection intensities higher than one nematode per fish in small larvae and post-larvae suggests that host survival may be affected by a high infection pressure. The relatively high levels of infection in the younger stages of cod, and the annual/spatial variability of these infections should be considered in the understanding of the early life dynamics of the species.

scholarly journals Fungi found in Mediterranean and North Sea sponges: how specific are they?

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3722 ◽  
Author(s):  
Mohd Azrul Naim ◽  
Hauke Smidt ◽  
Detmer Sipkema
Keyword(s):  
North Sea ◽  
Ribosomal Rna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Anaerobic Digesters ◽  
Operational Taxonomic Units ◽  
The North ◽  
Hypervariable Regions ◽  
The North Sea ◽  
Biological Sources

Fungi and other eukaryotes represent one of the last frontiers of microbial diversity in the sponge holobiont. In this study we employed pyrosequencing of 18S ribosomal RNA gene amplicons containing the V7 and V8 hypervariable regions to explore the fungal diversity of seven sponge species from the North Sea and the Mediterranean Sea. For most sponges, fungi were present at a low relative abundance averaging 0.75% of the 18S rRNA gene reads. In total, 44 fungal OTUs (operational taxonomic units) were detected in sponges, and 28 of these OTUs were also found in seawater. Twenty-two of the sponge-associated OTUs were identified as yeasts (mainly Malasseziales), representing 84% of the fungal reads. Several OTUs were related to fungal sequences previously retrieved from other sponges, but all OTUs were also related to fungi from other biological sources, such as seawater, sediments, lakes and anaerobic digesters. Therefore our data, supported by currently available data, point in the direction of mostly accidental presence of fungi in sponges and do not support the existence of a sponge-specific fungal community.

The North Sea: an overview

1987 ◽  
Vol 316 (1181) ◽  
pp. 461-485 ◽  
Keyword(s):  
Trace Metals ◽  
Organic Compounds ◽  
North Sea ◽  
Human Activities ◽  
Sea Water ◽  
Sea Floor ◽  
Natural Systems ◽  
The North ◽  
The North Sea ◽  
Trace Constituents

An overview is given of the natural systems of the North Sea: water-circulation, topography and geology of the sea floor, sediment transport, influx of trace constituents (nutrients, trace metals, organic compounds), biological systems and their interrelations. The effects of pollution and other human activities are discussed as well as the difficulties in assessing them where they are obscured by natural changes.

Quantitative studies on the marine Phycomycetes between Fladenground and the mouth of the River Tay

1980 ◽  
Vol 78 (3-4) ◽  
pp. s131-s131
Author(s):  
S. Raghu Kumar
Keyword(s):  
Surface Water ◽  
North Sea ◽  
Sea Water ◽  
Seasonal Fluctuation ◽  
Fungal Population ◽  
The North ◽  
Quantitative Studies ◽  
The North Sea ◽  
Very High ◽  
High Seas

SynopsisQuantitative studies on marine Phycomycetes (thraustochytrids) were carried out at 6 stations between the Fladenground area of the North Sea and the mouth of the River Tay, between September 1975 and September 1976. Five samplings were made of the surface water and the sediment during this period. The number of fungi/litre sea water or sediment increased with the proximity to the mouth of the river compared to the high seas. The fungal population exhibited a seasonal fluctuation, a high number occurring in September and an extremely low number occurring in March. The sediments revealed a very high number of propagules/litre (up to 73,000) compared to the water (up to 384).

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