scholarly journals Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

2012 ◽  
Vol 79 (3) ◽  
pp. 1052-1054 ◽  
Author(s):  
Dixie F. Mollenkopf ◽  
Jennifer M. Mirecki ◽  
Joshua B. Daniels ◽  
Julie A. Funk ◽  
Steven C. Henry ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Use ◽  
Fecal Samples ◽  
Content Type ◽  
Extended Spectrum ◽  
Lactamase Gene

ABSTRACTWe report the recovery ofEscherichia coliorKlebsiella pneumoniaecontaining the extended-spectrum β-lactamase geneblaCTX-Mfrom 24 of 1,495 (1.6%) swine fecal samples in 8 of 50 (16%) finishing barns located in 5 U.S. states. We did not detect an association between antimicrobial use and recovery ofblaCTX-M.

scholarly journals Novel Conjugative Plasmid from Escherichia coli of Swine Origin That Coharbors the Multiresistance Genecfrand the Extended-Spectrum-β-Lactamase GeneblaCTX-M-14b

2014 ◽  
Vol 59 (2) ◽  
pp. 1337-1340 ◽  
Author(s):  
Wan-Jiang Zhang ◽  
Xiu-Mei Wang ◽  
Lei Dai ◽  
Xin Hua ◽  
Zhimin Dong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Conjugative Plasmid ◽  
Content Type ◽  
Conjugative Plasmids ◽  
Extended Spectrum ◽  
Lactamase Gene

ABSTRACTTwo porcineEscherichia coliisolates harbored thecfrgene on conjugative plasmids of 38,405 bp (pGXEC6) and 41,646 bp (pGXEC3). In these two plasmids, thecfrgene was located within a 4,612-bp region containing atnpA-IS26-cfr-IS26-Δhypelement. Plasmid pGXEC3 was almost identical to pGXEC6 except for a 3,235-bp ISEcp1-blaCTX-M-14binsertion. The colocation of the multiresistancecfrgene with an extended-spectrum-β-lactamase gene on a conjugative plasmid may support the dissemination of these genes by coselection.

scholarly journals Carbapenem Therapy for Bacteremia Due to Extended-Spectrum-β-Lactamase-Producing Escherichia coli or Klebsiella pneumoniae: Implications of Ertapenem Susceptibility

2012 ◽  
Vol 56 (6) ◽  
pp. 2888-2893 ◽  
Author(s):  
Nan-Yao Lee ◽  
Ching-Chi Lee ◽  
Wei-Han Huang ◽  
Ko-Chung Tsui ◽  
Po-Ren Hsueh ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Content Type ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Medical Centers ◽  
Extended Spectrum ◽  
Appropriate Therapy ◽  
Related Mortality

ABSTRACTA retrospective study was conducted at two medical centers in Taiwan to evaluate the clinical characteristics, outcomes, and risk factors for mortality among patients treated with a carbapenem for bacteremia caused by extended-spectrum-beta-lactamase (ESBL)-producing organisms. A total of 251 patients with bacteremia caused by ESBL-producingEscherichia coliandKlebsiella pneumoniaeisolates treated by a carbapenem were identified. Among these ESBL-producing isolates, rates of susceptibility to ertapenem (MICs ≤ 0.25 μg/ml) were 83.8% and 76.4%, respectively; those to meropenem were 100% and 99.3%, respectively; and those to imipenem were 100% and 97.9%, respectively. There were no significant differences in the critical illness rate (P= 0.1) or sepsis-related mortality rate (P= 0.2) for patients with bacteremia caused by ESBL-producingK. pneumoniae(140 isolates, 55.8%) andE. coli(111 isolates, 44.2%). Multivariate analysis of variables related to sepsis-related mortality revealed that the presence of severe sepsis (odds ratio [OR], 15.9; 95% confidence interval [CI], 5.84 to 43.34;P< 0.001), hospital-onset bacteremia (OR, 4.65; 95% CI, 1.42 to 15.24;P= 0.01), and ertapenem-nonsusceptible isolates (OR, 5.12; 95% CI, 2.04 to 12.88;P= 0.001) were independent risk factors. The patients receiving inappropriate therapy had a higher sepsis-related mortality than those with appropriate therapy (P= 0.002), irrespective of ertapenem, imipenem, or meropenem therapy. Infections due to the ertapenem-susceptible isolates (MICs ≤ 0.25 μg/ml) were associated with a more favorable outcome than those due to ertapenem-nonsusceptible isolates (MICs > 0.25 μg/ml), if treated by a carbapenem. However, the mortality for patients with bacteremic episodes due to isolates with MICs of ≤0.5 μg/ml was similar to the mortality for those whose isolates had MICs of >0.5 μg/ml (P= 0.8). Such a finding supports the rationale of the current CLSI 2011 criteria for carbapenems forEnterobacteriaceae.

scholarly journals Characterization of CIA-1, an Ambler Class A Extended-Spectrum β-Lactamase from Chryseobacterium indologenes

2011 ◽  
Vol 56 (1) ◽  
pp. 588-590 ◽  
Author(s):  
Takehisa Matsumoto ◽  
Mika Nagata ◽  
Nau Ishimine ◽  
Kenji Kawasaki ◽  
Kazuyoshi Yamauchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reference Strain ◽  
Content Type ◽  
Chryseobacterium Indologenes ◽  
Esbl Gene ◽  
Class A ◽  
Extended Spectrum ◽  
Class B ◽  
Lactamase Gene

ABSTRACTAn Ambler class A β-lactamase gene,blaCIA-1, was cloned from the reference strainChryseobacterium indologenesATCC 29897 and expressed inEscherichia coliBL21. TheblaCIA-1gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively.blaCIA-1-like genes were detected from clinical isolates. In addition to the metallo-β-lactamase IND of Ambler class B,C. indologeneshas a class A ESBL gene,blaCIA-1, located on the chromosome.

scholarly journals Complete Nucleotide Sequence of an IncI2 Plasmid CoharboringblaCTX-M-55andmcr-1

2016 ◽  
Vol 60 (8) ◽  
pp. 5014-5017 ◽  
Author(s):  
Jian Sun ◽  
Xing-Ping Li ◽  
Run-Shi Yang ◽  
Liang-Xing Fang ◽  
Wei Huo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Sequence Similarity ◽  
Conjugative Plasmid ◽  
Insertion Sequences ◽  
Content Type ◽  
Extensive Sequence ◽  
Extended Spectrum ◽  
Lactamase Gene

ABSTRACTWe report the complete nucleotide sequence of a plasmid, pA31-12, carryingblaCTX-M-55andmcr-1from a chickenEscherichia coliisolate. pA31-12 has an IncI2 replicon that displays extensive sequence similarity with pHN1122-1-borneblaCTX-M-55and pHNSHP45-bornemcr-1. Insertion sequences ISEcp1and ISApl1are responsible for the mobilization ofblaCTX-M-55andmcr-1, respectively. The colocalization ofmcr-1with an extended-spectrum β-lactamase gene on a conjugative plasmid may accelerate the dissemination of both genes by coselection.

scholarly journals Dominance of blaCTX-M within an Australian Extended-Spectrum β-Lactamase Gene Pool

2008 ◽  
Vol 52 (11) ◽  
pp. 4198-4202 ◽  
Author(s):  
Zhiyong Zong ◽  
Sally R. Partridge ◽  
Lee Thomas ◽  
Jonathan R. Iredell
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Gene Pool ◽  
Clinical Isolates ◽  
Conjugative Plasmids ◽  
Extended Spectrum ◽  
Diverse Community ◽  
Lactamase Gene ◽  
Sydney Australia

ABSTRACT bla CTX-M genes, particularly bla CTX-M-15, are the dominant extended-spectrum β-lactamase (ESBL) genes among clinical isolates of Escherichia coli and Klebsiella pneumoniae in Sydney, Australia, where we also found one example of bla CTX-M-62, encoding a novel enzyme conferring ceftazidime resistance. ESBL genes were present in diverse community isolates and in a variety of associated conjugative plasmids.

scholarly journals Molecular Identification of Extended-Spectrum-β-Lactamase Genes from Enterobacteriaceae Isolated from Healthy Human Carriers in Switzerland

2011 ◽  
Vol 56 (3) ◽  
pp. 1609-1612 ◽  
Author(s):  
Nadine Geser ◽  
Roger Stephan ◽  
Bozena M. Korczak ◽  
Lothar Beutin ◽  
Herbert Hächler
Keyword(s):  
Escherichia Coli ◽  
Pcr Analysis ◽  
Healthy Human ◽  
Fecal Samples ◽  
Content Type ◽  
Healthy Humans ◽  
Extended Spectrum ◽  
High Degree ◽  
Esbl Producers ◽  
M 14

ABSTRACTIn this study, fecal samples from 586 healthy humans were investigated to determine the occurrence of extended-spectrum-β-lactamase (ESBL)-producingEnterobacteriaceaein Swiss people. A total of 5.8% of the human fecal samples yielded ESBL producers, and all of the 34 isolated strains wereEscherichia coli. PCR analysis revealed that 14 strains produced CTX-M-15, 10 produced CTX-M-1, 7 strains produced CTX-M-14, and 2 strains produced CTX-M-2 ESBLs. One strain produced SHV-12 ESBL. Of the 34 isolates, 15 produced additional TEM-1 broad-spectrum β-lactamases. By serotyping, a high degree of diversity among the strains was found.

scholarly journals Complete Genome Sequences of Two Phage-Like Plasmids Carrying the CTX-M-15 Extended-Spectrum β-Lactamase Gene

2017 ◽  
Vol 5 (19) ◽  
Author(s):  
Anna Colavecchio ◽  
Julie Jeukens ◽  
Luca Freschi ◽  
Jean-Guillaume Edmond Rheault ◽  
Irena Kukavica-Ibrulj ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Complete Genome ◽  
Nucleotide Sequence Identity ◽  
Genome Sequences ◽  
Content Type ◽  
Sequence Identity ◽  
Extended Spectrum ◽  
Lactamase Gene

ABSTRACT Two similar phage-like plasmids carrying CTX-M-15 resistance cassettes were identified from two environmental Escherichia coli isolates. They demonstrate strong nucleotide sequence identity to the phage-like plasmid pECOH89 and Salmonella bacteriophage SSU5.

scholarly journals Wild Boars Carry Extended-Spectrum β-Lactamase- and AmpC-Producing Escherichia coli

2021 ◽  
Vol 9 (2) ◽  
pp. 367 ◽  
Author(s):  
Anna R. Holtmann ◽  
Diana Meemken ◽  
Anja Müller ◽  
Diana Seinige ◽  
Kathrin Büttner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Phylogenetic Groups ◽  
Fecal Samples ◽  
Wild Boars ◽  
E Coli ◽  
Extended Spectrum ◽  
Nasal Swabs ◽  
Typing Methods ◽  
Lactamase Gene

Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) represent major healthcare concerns. The role of wildlife in the epidemiology of these bacteria is unclear. The purpose of this study was to determine their prevalence in wild boars in Germany and to characterize individual isolates. A total of 375 fecal samples and 439 nasal swabs were screened for the presence of ESBL-/AmpC-E. coli and MRSA, respectively. The associations of seven demographic and anthropogenic variables with the occurrence of ESBL-/AmpC-E. coli were statistically evaluated. Collected isolates were subjected to antimicrobial susceptibility testing, molecular typing methods, and gene detection by PCR and genome sequencing. ESBL-/AmpC-E. coli were detected in 22 fecal samples (5.9%) whereas no MRSA were detected. The occurrence of ESBL-/AmpC-E. coli in wild boars was significantly and positively associated with human population density. Of the 22 E. coli, 19 were confirmed as ESBL-producers and carried genes belonging to blaCTX-M group 1 or blaSHV-12. The remaining three isolates carried the AmpC-β-lactamase gene blaCMY-2. Several isolates showed additional antimicrobial resistances. All four major phylogenetic groups were represented with group B1 being the most common. This study demonstrates that wild boars can serve as a reservoir for ESBL-/AmpC-producing and multidrug-resistant E. coli.

scholarly journals Tigecycline DisplaysIn VivoBactericidal Activity against Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae after 72-Hour Exposure Period

2012 ◽  
Vol 57 (1) ◽  
pp. 640-642 ◽  
Author(s):  
Pamela R. Tessier ◽  
David P. Nicolau
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Clinical Outcomes ◽  
Bactericidal Activity ◽  
Exposure Period ◽  
Content Type ◽  
Density Reduction ◽  
Extended Spectrum ◽  
Level Of Activity

ABSTRACTProgressively enhanced activity of a humanized tigecycline (TGC) regimen was noted over 3 days against an extended-spectrum-β-lactamase (ESBL)-producingEscherichia coliisolate and an ESBL-producingKlebsiella pneumoniaeisolate. Bacterial density reduction approximated 3 log10approaching bactericidal activity at 72 h. This level of activity has not been previously noted for compounds such as tetracyclines, normally considered bacteriostatic antimicrobials. Extended regimen studiesin vivomay aid in better delineation of antimicrobial effects, producing improved correlation with clinical outcomes.

scholarly journals Mutations in blaKPC-3 That Confer Ceftazidime-Avibactam Resistance Encode Novel KPC-3 Variants That Function as Extended-Spectrum β-Lactamases

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
Ghady Haidar ◽  
Cornelius J. Clancy ◽  
Ryan K. Shields ◽  
Binghua Hao ◽  
Shaoji Cheng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Content Type ◽  
Extended Spectrum

ABSTRACT We identified four bla KPC-3 mutations in ceftazidime-avibactam-resistant clinical Klebsiella pneumoniae isolates, corresponding to D179Y, T243M, D179Y/T243M, and EL165-166 KPC-3 variants. Using site-directed mutagenesis and transforming vectors into Escherichia coli, we conclusively demonstrated that mutant bla KPC-3 encoded enzymes that functioned as extended-spectrum β-lactamases; mutations directly conferred higher MICs of ceftazidime-avibactam and decreased the MICs of carbapenems and other β-lactams. Impact was strongest for the D179Y mutant, highlighting the importance of the KPC Ω-loop.

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