scholarly journals Recombinant Multiepitope Protein for Early Detection of Dengue Infections

2006 ◽  
Vol 13 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Ravulapalli AnandaRao ◽  
Sathyamangalam Swaminathan ◽  
Sirimali Fernando ◽  
Asha M. Jana ◽  
Navin Khanna
Keyword(s):  
Dengue Fever ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Laboratory Diagnosis ◽  
Viral Disease ◽  
M Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Fusion ◽  
Promising Alternative ◽  
Strip Test

ABSTRACT Dengue fever is a mosquito-borne viral disease prevalent mainly in tropical countries. As the clinical manifestations of dengue are not very unique, laboratory diagnosis is crucial in identifying cases of dengue infection. Detection of dengue infection based on the identification of antidengue antibodies has emerged as a practical and reliable means of diagnosing dengue fever. We recently developed a customized recombinant dengue multiepitope protein (r-DME-G) that can specifically detect the immunoglobulin G (IgG) class of antidengue antibodies in patient sera. Using this strategy, we have now created another dengue multiepitope protein, r-DME-M, with specificity for the IgM class of antidengue antibodies. A synthetic gene encoding the r-DME-M protein was expressed as a maltose-binding protein fusion in Escherichia coli. The recombinant protein was purified in a single affinity chromatographic step to obtain yields of ∼15 mg purified protein/liter of culture. The purified protein was used to develop an in-house IgM enzyme-linked immunosorbent assay (ELISA) and tested using a panel of 172 patient sera characterized using the commercially available Dengue Duo rapid strip test from PanBio, Australia. The IgM ELISA results showed that the r-DME-M protein not only recognized all IgM+ samples identified by the PanBio test but also identified samples missed by the latter test. We also successfully adapted the r-DME-M protein to a rapid strip test format. This approach of creating customized antigens coupled to overexpression in E. coli and simple purification offers a promising alternative option to dengue diagnosis with the potential to circumvent the drawbacks of the whole virus antigen-based commercial kits.

scholarly journals Presentation, Management and Outcome of Dengue Fever – A Study of 200 Cases

2013 ◽  
Vol 14 (1) ◽  
pp. 18-22
Author(s):  
Md. Habibur Raman ◽  
Abu Yousuf Md. Shahidul Alam ◽  
AKM Mijanur Rahman ◽  
Md. Sarwar Khan ◽  
Nahid Reaz Shapla ◽  
...  
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Skin Rash ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Case Fatality ◽  
Blood Platelet ◽  
Viral Disease ◽  
Female Ratio ◽  
General Weakness

Background: Dengue is the most rapidly spreading mosquito-borne viral disease in the world1. The rapidly expanding global footprint of dengue is a public health challenge. The endemicity of dengue is also increasing in Bangladesh. This study highlights our current understanding of dengue, including its clinical manifestations, laboratory tests, management and outcome. Objectives: This study was designed to document the presenting features and outcome of Dengue infection in Border Guard personnel. Materials and Methods: It was a prospective observational study which was carried out among outpatient and indoor cases from February 2011 to November 2012 in Border Guard Hospital, Dhaka which is a 300 bedded hospital. Total 200 cases were enrolled. A detailed history, clinical examinations and relevant investigations were done. Data were collected in a predesigned structured questionnaire and analyzed with the help of SPSS-16.0 and Chisquare (X2) Test. Results: A total of 200 adult seropositive Dengue cases of various grade were studied. Among these 152(76%) were male and 48 (24%) were female. Male to female ratio was 3.17:1.The age range of the patients was 18 to 60 years and the mean age 39±12.56 years. Among 200 patients, 112(66%) were Dengue Fever (DF) and 88(44%) were Dengue Haemorrhagic Fever (DHF) including 3(1.5%) cases of DHF Grade lII but none (0%) had Grade-IV DHF. All the patients presented with fever 200(100%), general weakness 200(100%) followed by various skin rash 196(98%), headache 192(96%), myalgia/arthralgia 191(95.5%), retroorbital pain 84(42%). Bleeding manifestation showed in 94(47%) cases of which petechiae was most frequent 86(43%), Haematocrit was normal only in 13(6.5%) patients and 82(41%) had a rise of >20%; Leucopenia was found in 187(93.5%) patients.Only 2(1%) patients had normal platelet count and 03(1.5%) patients had platelet count of less than 10X109 /L. Raised serum alanine aminotransferase (ALT) was observed in 184(92%) of cases. All (200%) the patients recovered completely from the disease; however, one patient subsequently developed Guillein Barre Syndrome. Conclusion: High persistent fever, profound general weakness, myalgia, headache and itchy skin rash were the usual presenting features. Most of the patients recovered well with efficient symptomatic and supportive treatment. Very few cases required blood/platelet transfusion. There was no case fatality in this study group DOI: http://dx.doi.org/10.3329/jom.v14i1.14531 J MEDICINE 2013; 14 : 18-22

scholarly journals Epidemiological and Clinical Features of Dengue Infection in Adults in the 2017 Outbreak in Vietnam

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Bui Vu Huy ◽  
Le Nguyen Minh Hoa ◽  
Dang Thi Thuy ◽  
Nguyen Van Kinh ◽  
Ta Thi Dieu Ngan ◽  
...  
Keyword(s):  
Elderly Patients ◽  
Dengue Fever ◽  
Clinical Features ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Warning Signs ◽  
Severe Dengue ◽  
Severe Bleeding ◽  
Cross Sectional ◽  
Number Of Patients

Purpose. The clinical features and laboratory results of dengue-infected adult patients admitted to the hospital during the 2017 outbreak were analyzed in this study. Method. This is a cross-sectional study. 2922 patients aged 18 years or more with dengue fever in National Hospital for Tropical Diseases (NHTD) in the North and Hospital for Tropical Disease (HTD) in the South of Vietnam were recruited in this study. Result. Patients were admitted in the hospital around the year and concentrated from August to December, in 53/63 (84.0%) provinces in Vietnam, and patients in all ages were affected. The number of patients with dengue fever was 1675 (57.3%), dengue with warning signs 914 (31.3%), and severe dengue 333 (11.4%), respectively. Among patients with severe dengue, severe plasma leakage and dengue shock account for 238 (8.1%), severe organ impairment 73 (2.5%), and severe bleeding 22 (0.75%). The rate of mortality was 0.8%, and the outcome of dengue patients is worse in the elderly and people with underlying diseases. Conclusion. The 2017 dengue outbreak occurred in a larger scale than in the previous years in terms of time, location, and number of patients. More elderly patients were infected by dengue in this outbreak, and this may contribute to the mortality rate. Clinical manifestations of dengue patients in Southern Vietnam are more typical than the northern, but the rate of severe dengue is not different. The mortality risk and underlying conditions associated with dengue-infected elderly patients are worthy of further investigations in the future.

scholarly journals The seasonal reproduction number of dengue fever impacts of climate on transmission

2016 ◽  
Author(s):  
Sittisede Polwiang
Keyword(s):  
Low Temperature ◽  
Dengue Fever ◽  
Rainy Season ◽  
Reproduction Number ◽  
Dengue Infection ◽  
Viral Disease ◽  
Seasonal Reproduction ◽  
Epidemic Period ◽  
Very High ◽  
Very Low Temperature

Background: The dengue fever is a mosquito-borne viral disease and a regular epidemic in Thailand. The peak of the dengue epidemic period is around June to August during the rainy season. It is believed that the climate is an important factor for dengue transmission.Method: A mathematical model for vector-host infectious disease was used to calculate the impacts of climate to the transmission of dengue virus. In this study, the data of climate and dengue fever cases were derived from Chiang Mai during 2008-2015, Thailand. The value of seasonal reproduction number was calculated to evaluate the potential, severity and persistence of dengue infection.Results: The mosquito population was increasing exponentially from the start of the rainy season in early May and reached its the peak in late June. The simulations suggest that the greatest potential for the dengue transmission occurs when the temperature is 28.9ºC. The seasonal reproduction numbers were larger than one from late March to end of August and reaching the peak in June. The highest incidences occurred in August due to the delay of transmission humans-mosquito-humans. Increasing mean temperature by 1.2ºC, the number of incidences increases 43.7%. However, a very high or very low temperature reduces the number of infection.Discussion and Conclusion: The results show that the dengue infection depends on the seasonal variation of the climate. The rainfall provides places for the mosquitoes to lay eggs and develop to adult stage. The temperature plays an important role in the life cycle and behavior of the mosquitoes. A very high or very low temperature reduces the risk of the dengue infection.

scholarly journals Frequency and Clinical Manifestations of Dengue in Urban Medellin, Colombia

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Berta Nelly Restrepo ◽  
Mark E. Beatty ◽  
Yenny Goez ◽  
Ruth E. Ramirez ◽  
G. William Letson ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Low Income ◽  
Dengue Infection ◽  
Diagnostic Testing ◽  
Clinical Manifestations ◽  
Febrile Illness ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Medical Facilities

A dengue fever surveillance study was conducted at three medical facilities located in the low-income district of San Javier in Medellin, Colombia. During March 2008 to 2009, 781 patients with fever regardless of chief complaint were recruited for acute dengue virus infection testing. Of the 781 tested, 73 (9.3%) were positive for dengue infection. Serotypes DENV-2 (77%) and -3 (23%) were detected by PCR. One patient met the diagnostic criteria for dengue hemorrhagic fever. Only 3 out of 73 (4.1%) febrile subjects testing positive for dengue infection were diagnosed with dengue fever by the treating physician. This study confirms dengue virus as an important cause of acute febrile illness in Medellin, Colombia, but it is difficult to diagnose without dengue diagnostic testing.

scholarly journals Clinical-epidemiological characteristics delivery cases of tropic dengue fever

2021 ◽  
Vol 13 (1) ◽  
pp. 78-85
Author(s):  
V. V. Nechaev ◽  
I. I. Yarovaya ◽  
G. V. Kachenya ◽  
E. V. Doguzhieva ◽  
S. S. Buntovskya ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Clinical Manifestations ◽  
Hemorrhagic Fever ◽  
Viral Disease ◽  
Primary Diagnosis ◽  
West Nile Fever ◽  
Tropical Countries ◽  
Number Of Patients ◽  
Epidemiological Characteristics ◽  
The City

Dengue fever is a zooantroponotic, vector-borne viral disease. It is common among the population of tropical countries and is characterized by a tendency to expand to other countries as a result of infection drifts.Objective: to identify epidemiological, clinical and laboratory features of imported dengue fever in St. Petersburg.Materials and methods. The obtained data of official registration of cases of diseases in the Department of accounting and registration of infectious and parasitic diseases of the city of St. Petersburg and clinical case histories from the Сlinical Infectious diseases hospital named after S.P. Botkin for 2012–2018 were used as the basis for epidemiological analysis. 144 cases of dengue fever were identified on the basis of registration materials in the city during this period, of which 86 cases were studied on clinical histories. Standards methods of epidemiological diagnostics and statistics were used in the article.Results and discussion. Epidemiological anamnesis has been seen in a wide variety of countries in which tourists stayed and became infected. The hemorrhagic form of dengue fever was diagnosed in only 18 (19,1%) out of 86 patients. This form usually develops with repeated encounters with the virus and is more severe than the classic version of the disease. Dengue fever remains a topical imported disease and is associated with an increase in tourists, often re-visiting disadvantaged tropical countries. Both sexes were equally exposed to the disease. The age characteristic of patients is represented by persons of 20–29 and 30–39 years of age. An increase in the number of patients over a 7-year period was noted in May and November. Such countries as Thailand (37%), Vietnam (13,9%) and India (9,3%) were the predominant tourist destinations. Besides, single infections occurred in 13 countries of the Asian, African and American regions. The primary diagnosis in 50,3% of patients referred to hospital were acute respiratory diseases, combined with diarrhea and other manifestations. Clinical manifestations fit into 5 syndromes, which pass with a variety of symptoms, complicating both clinical diagnosis and laboratory confirmation of the diagnosis. Imported dengue fever is characterized by combined infections caused by pathogens of acute intestinal diseases, cytomegalovirus, hemorrhagic fever with renal syndrome, West Nile fever, hepatitis A, Legionella and Plasmodium malaria. In General, combined infection was detected in 39.5% of patients.

scholarly journals The seasonal reproduction number of dengue fever impacts of climate on transmission

2015 ◽  
Author(s):  
Sittisede Polwiang
Keyword(s):  
Low Temperature ◽  
Dengue Fever ◽  
Rainy Season ◽  
Reproduction Number ◽  
Dengue Infection ◽  
Viral Disease ◽  
Seasonal Reproduction ◽  
Epidemic Period ◽  
Very High ◽  
Very Low Temperature

Background: The dengue fever is a mosquito-borne viral disease and a regular epidemic in Thailand. The peak of the dengue epidemic period is around June to August during the rainy season. It is believed that the climate is an important factor for dengue transmission.Method: A mathematical model for vector-host infectious disease was used to calculate the impacts of climate to the transmission of dengue virus. In this study, the data of climate and dengue fever cases were derived from Chiang Mai during 2004-2014, Thailand. The value of seasonal reproduction number was calculated to evaluate the potential, severity and persistence of dengue infection.Results: The mosquito population was increasing exponentially from the start of the rainy season in early May and reached its the peak in late June. The simulations suggest that the greatest potential for the dengue transmission occurs when the temperature is 28.7ºC. The seasonal reproduction numbers were larger than one from late March to end of August and reaching the peak in June. The highest incidences occurred in August due to the delay of transmission humans-mosquito-humans. Increasing mean temperature by 1ºC, the number of incidences increases 30%. However, a very high or very low temperature reduces the number of infection.Discussion and Conclusion: The results show that the dengue infection depends on the seasonal variation of the climate. The rainfall provides places for the mosquitoes to lay eggs and develop to adult stage. The temperature plays an important role in the life cycle and behavior of the mosquitoes. A very high or very low temperature reduces the risk of the dengue infection.

scholarly journals The seasonal reproduction number of dengue fever impacts of climate on transmission

2016 ◽  
Author(s):  
Sittisede Polwiang
Keyword(s):  
Low Temperature ◽  
Dengue Fever ◽  
Rainy Season ◽  
Reproduction Number ◽  
Dengue Infection ◽  
Viral Disease ◽  
Seasonal Reproduction ◽  
Epidemic Period ◽  
Very High ◽  
Very Low Temperature

Background: The dengue fever is a mosquito-borne viral disease and a regular epidemic in Thailand. The peak of the dengue epidemic period is around June to August during the rainy season. It is believed that the climate is an important factor for dengue transmission.Method: A mathematical model for vector-host infectious disease was used to calculate the impacts of climate to the transmission of dengue virus. In this study, the data of climate and dengue fever cases were derived from Chiang Mai during 2008-2015, Thailand. The value of seasonal reproduction number was calculated to evaluate the potential, severity and persistence of dengue infection.Results: The mosquito population was increasing exponentially from the start of the rainy season in early May and reached its the peak in late June. The simulations suggest that the greatest potential for the dengue transmission occurs when the temperature is 28.9ºC. The seasonal reproduction numbers were larger than one from late March to end of August and reaching the peak in June. The highest incidences occurred in August due to the delay of transmission humans-mosquito-humans. Increasing mean temperature by 1.2ºC, the number of incidences increases 43.7%. However, a very high or very low temperature reduces the number of infection.Discussion and Conclusion: The results show that the dengue infection depends on the seasonal variation of the climate. The rainfall provides places for the mosquitoes to lay eggs and develop to adult stage. The temperature plays an important role in the life cycle and behavior of the mosquitoes. A very high or very low temperature reduces the risk of the dengue infection.

Study of Cardiac Manifestation in Patients with Seropositive Dengue Fever and to Find Correlation with Subclinical Cardiac Involvement

2021 ◽  
Vol 8 (12) ◽  
pp. 28-33
Author(s):  
Rishad Ahmed
Keyword(s):  
Dengue Fever ◽  
Cardiac Involvement ◽  
Randomized Study ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Denv Infection ◽  
Left Ventricular ◽  
High Grade Fever ◽  
Plasma Leakage ◽  
Cardiac Manifestation

Objective: The main objective of the present study was to observe the electrocardiogram (ECG) changes in dengue fever and to find correlation with subclinical cardiac involvement. Methods: This was an observational, prospective, non-randomized study. High grade fever for one to five days diagnosed as dengue infection later confirmed by dengue tests (NS1, IgG and IgM ELISA) were included in this study. Prior to the commencement of the study informed consent was taken from all the participants. With 12 lead ECG taken during the febrile phase of dengue fever randomly selected serologically confirmed cases of dengue fever were evaluated at an interval of 24 hrs. for a total of five days. On the day of admission when the patient was afebrile, echocardiography was done. Plasma leakage was diagnosed by presence of any one of four: subcutaneous oedema (pedal oedema, facial puffiness) or pleural effusion or pericardial effusion or ascites. Result: A total 80 patients who were admitted to hospital during study periods due to symptoms of dengue fever and IgM dengue positive status were included in this study. Among this total population of 80 patients (N=80) 48 were male and 32 were female. Out of these, majority were diagnosed as DF 52 (65%), 23 (29%) were diagnosed as DHF and rest only 5 (6%) were diagnosed as DSS. Total 54 patients were found to have normal ECG among which 35 were having dengue fever, 16 were having DHF and 3 were having DSS. Total 2 patients were diagnosed to have left ventricular hypertrophy among which one had dengue fever and rest two had DHF. ST depression was observed in total 7 patients in which 5 were had dengue fever and 2 were had DHF. Relative bradycardia was found in 14, tall t waves in 2 and ventricular ectopics in 1 patient (table 2). Abnormal ECG findings were compared using Chi-square test and were found to be statistically significant (P < 0.05) in dengue fever and DHF. There was statistically significant correlation between ECG abnormalities and abdominal pain and mucosal bleed. The presence of plasma leakage were observed among 77 (96.3%) patients. Proportion of echocardiography abnormalities were higher among patients with plasma leak 6 (30%) as compared to without plasma leak 9 (15%), this difference was not found to be statistically significant Conclusion: In all type of Dengue infections incidence of cardiac involvement was quite high. Due to the overlapping clinical manifestations such as capillary leak associated with DENV infection, tachycardia, pulmonary edema, and hypotension as well as due to the low index of clinical suspicion cardiac involvement in dengue fever is often underdiagnosed. Keywords: Cardiac manifestation, dengue infection, echocardiography, electrocardiography.

scholarly journals Dengue viral infection deteriorates vitamin D3, K, thrombopoietin, and angiotensinogen levels in humans

2018 ◽  
Vol 16 ◽  
pp. 205873921879110 ◽  
Author(s):  
Hina Fatima ◽  
Muhammad Riaz ◽  
Zahed Mahmood ◽  
Fatima Yousaf ◽  
Muhammad Shahid
Keyword(s):  
Dengue Fever ◽  
Hematological Parameters ◽  
Test Group ◽  
Serum Levels ◽  
Viral Disease ◽  
Blood Parameters ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Mortality And Morbidity ◽  
Elisa Kit

Dengue fever is the most significant arthropod-transmitted viral disease in terms of mortality and morbidity, affecting at least 50 million people around the world. It is commonly recognized as “breakbone” fever as it is mostly associated with severe muscle and joint pain. The objective of the study was to evaluate and assess the levels of selected vitamins and hormones in serum of dengue patients from Faisalabad, Pakistan. Estimation of serum levels of vitamins D3 and K was carried out through spectrophotometer, while concentration of serum hormones, angiotensinogen and thrombopoietin, was determined through enzyme-linked immunosorbent assay (ELISA) kit method. Hematological blood parameters were also investigated. Hematological parameters significantly ( P < 0.05) differ in dengue fever patients and normal control subjects. Vitamin D3 level was found significantly ( P < 0.05) decreased in test group (dengue patients) compared to control group (healthy individual). Vitamin K level was also reduced in test group compared to control group, but this reduction was nonsignificant ( P > 0.05). Among the tested hormones, angiotensinogen level was found significantly ( P < 0.05) lower in test group, while thrombopoietin was nonsignificantly ( P > 0.05) higher compared to control group. In conclusion, the results of the current study demonstrated the significance of tested vitamins and hormones along with blood parameters in monitoring the dengue infected patients.

FACTORS PREDICTING SEVERITY OF ILLNESS IN DENGUE FEVER: AN OBSERVATIONAL STUDY

2021 ◽  
pp. 54-55
Author(s):  
Abhay John Gray ◽  
Sheela Mathew ◽  
Gayathri R ◽  
Akhila Arya ◽  
Aswathy MA ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Ldl Cholesterol ◽  
Severe Disease ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Severity Of Illness ◽  
Warning Signs ◽  
Severe Dengue ◽  
Detailed History ◽  
Dengue With Warning Signs

Introduction: Dengue is a systemic viral infection caused by the virus from genus Flaviviridae. Dengue infection has got a wide clinical spectrum that includes both severe and non-severe clinical manifestations. The group progressing from non-severe to severe disease is difcult to dene. Early detection and access to proper medical care signicantly lower fatality rates and would help to reduce the burden of hospital stay and economy loss. Objectives: To study the inammatory markers predicting the severity of dengue infection. Methods: 250 diagnosed patients were evaluated with detailed history, physical examination and blood investigations. Inammatory markers associated with severity of dengue were studied. Observations and conclusions- 97 patients (39%) had dengue without warning signs, 62(25%) had dengue with warning signs, 63(25%) had severe dengue and 28(11%) had expanded dengue. Low CRP, ESR, HDL and LDL cholesterol were observed in dengue fever, without any statistical signicance. Ferritin levels more than 1000 and triglyceride more than 200 was signicantly associated with severity of dengue.

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