Frequency and Clinical Manifestations of Dengue in Urban Medellin, Colombia

A dengue fever surveillance study was conducted at three medical facilities located in the low-income district of San Javier in Medellin, Colombia. During March 2008 to 2009, 781 patients with fever regardless of chief complaint were recruited for acute dengue virus infection testing. Of the 781 tested, 73 (9.3%) were positive for dengue infection. Serotypes DENV-2 (77%) and -3 (23%) were detected by PCR. One patient met the diagnostic criteria for dengue hemorrhagic fever. Only 3 out of 73 (4.1%) febrile subjects testing positive for dengue infection were diagnosed with dengue fever by the treating physician. This study confirms dengue virus as an important cause of acute febrile illness in Medellin, Colombia, but it is difficult to diagnose without dengue diagnostic testing.

Download Full-text

The Role Activity of Complement, TNFα & IL12 in Pathogenesis Dengue Virus

Indonesian Journal of Tropical and Infectious Disease ◽

10.20473/ijtid.v1i1.3723 ◽

2010 ◽

Vol 1 (1) ◽

pp. 44

Author(s):

Soegeng Soegijanto ◽

Dian Dwi Sary ◽

Budi Setiawan ◽

Atsushi Yamanaka

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Dengue Fever ◽

Dengue Hemorrhagic Fever ◽

Hemorrhagic Fever ◽

Study Groups ◽

Dengue Virus Infection ◽

Cross Sectional ◽

Complement Activity ◽

Grade Iii

Dengue Virus infection is always found in some part of the world especially South East Asia including Indonesia. The pathogenesis of Dengue Virus infection is still controversial. The aim of this study is to analyze the role complement activity, TNFα & IL12 in Dengue Virus infection especially in pathogenesis of Dengue Virus infection. Cross sectional study had been done since February 2009 in Dr. Soetomo Hospital Surabaya. Blood Sera of Dengue Virus infection were collected from Dengue Fever, and Dengue Hemorrhagic Fever patient who had been care in Paediatric. Dengue patients and time schedule for taking blood sample for examination CH50, TNFα & IL12 as follow: on the first day on admission, the second day, the third day. Study groups of patients as follow: Dengue Fever, 36; Dengue Hemorrhagic Fever grade I, 37; Dengue Hemorrhagic Fever grade II, 10; Dengue Hemorrhagic Fever grade III, 18; Dengue Hemorrhagic Fever grade IV, 6. In this study found that the higher activity complement which lower level CH50 was more identified on Dengue Shock Syndrome and Dengue Hemorrhagic Fever grade III than Dengue Fever cases. A concept of our study was focusing on manifestation of vascular leakage, measurement of complement activity CH50, TNFα & IL12 and clinical manifestation Dengue Hemorrhagic Fever. The examination of TNFα & IL12 in our study supported the role the activity complement. The conclusion are measurement CH50, TNFα & IL12 can be used as a predictive factor of the degree of Dengue Virus infection

Download Full-text

Addition of Partial Envelope Domain II into Envelope Domain III of Dengue Virus Antigen Potentiates the Induction of Virus-Neutralizing Antibodies and Induces Protective Immunity

Vaccines ◽

10.3390/vaccines8010088 ◽

2020 ◽

Vol 8 (1) ◽

pp. 88 ◽

Cited By ~ 3

Author(s):

Jisang Park ◽

Hyun-Young Lee ◽

Ly Tuan Khai ◽

Nguyen Thi Thu Thuy ◽

Le Quynh Mai ◽

...

Keyword(s):

Dengue Virus ◽

Dengue Fever ◽

Neutralizing Antibodies ◽

Subunit Vaccine ◽

Secondary Infection ◽

Febrile Illness ◽

Hemorrhagic Fever ◽

Denv Infection ◽

Severe Dengue ◽

Domain Iii

Dengue virus (DENV) comprises four serotypes in the family Flaviviridae and is a causative agent of dengue-related diseases, including dengue fever. Dengue fever is generally a self-limited febrile illness. However, secondary infection of patients with a suboptimal antibody (Ab) response provokes life-threatening severe dengue hemorrhagic fever or dengue shock syndrome. To develop a potent candidate subunit vaccine against DENV infection, we developed the EDII-cEDIII antigen, which contains partial envelope domain II (EDII) including the fusion loop and BC loop epitopes together with consensus envelope domain III (cEDIII) of all four serotypes of DENV. We purified Ab from mice after immunization with EDII-cEDIII or cEDIII and compared their virus neutralization and Ab-dependent enhancement of DENV infection. Anti-EDII-cEDIII Ab showed stronger neutralizing activity and lower Ab-dependent peak enhancement of DENV infection compared with anti-cEDIII Ab. Following injection of Ab-treated DENV into AG129 mice, anti-EDII-cEDIII Ab ameliorated DENV infection in tissues with primary and secondary infection more effectively than anti-cEDIII Ab. In addition, anti-EDII-cEDIII Ab protected against DENV1, 2, and 4 challenge. We conclude that EDII-cEDIII induces neutralizing and protective Abs, and thus, shows promise as a candidate subunit vaccine for DENV infection.

Download Full-text

The 1993 dengue 2 epidemic in Charters Towers, North Queensland: clinical features and public health impact

Epidemiology and Infection ◽

10.1017/s0950268898001058 ◽

1998 ◽

Vol 121 (1) ◽

pp. 151-156 ◽

Cited By ~ 26

Author(s):

W. J. H. McBRIDE ◽

H. MULLNER ◽

J. T. LaBROOY ◽

I. WRONSKI

Keyword(s):

Dengue Virus ◽

Dengue Fever ◽

Dengue Infection ◽

Neutralizing Antibody ◽

Symptomatic Patient ◽

Health Impact ◽

Subclinical Infection ◽

Dengue Virus Infection ◽

Dengue Virus Type 2

In 1993 an epidemic caused by dengue virus type 2 occurred in several North Queensland population centres. Charters Towers, estimated population 10000, had 155 officially notified cases. An analysis of symptoms was undertaken using a random sample of 1000 residents to determine specificity of symptoms, the subclinical infection rate, and to establish the true extent of the epidemic. Retrospective diagnoses of dengue fever were based on the presence of both serum dengue 2 neutralizing antibody and presence of symptoms. An estimated 20% of the population had dengue fever. The rate of subclinical infections in this epidemic was 14·6%. There were no symptoms that were specific for dengue fever. Bleeding occurred more frequently in people who recalled a previous dengue infection during a dengue 1 epidemic 12 years earlier (55·6% vs. 16·8%, P=0·003). Surveillance for future epidemics should be based on serological and virological confirmation of dengue virus infection amongst symptomatic patient.

Download Full-text

Clinico-haematological profile of dengue virus infection at rural tertiary care centre in North India

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20173080 ◽

2017 ◽

Vol 5 (8) ◽

pp. 3329

Author(s):

Vidyasagar Ram ◽

Khwaja Saifullah Zafar ◽

Manoj Kumar ◽

Ganga Prasad Vaishya ◽

Ejaz Ahmad ◽

...

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Dengue Fever ◽

Indian Subcontinent ◽

Dengue Infection ◽

Tertiary Care ◽

Laboratory Data ◽

North India ◽

Dengue Virus Infection ◽

Haematological Profile

Background: In India, dengue epidemics are becoming more frequent. Dengue viral infections are among the most important mosquito-borne disease of the Indian subcontinent. The study aimed to compare the clinical and haematological profile of all patients diagnosed with dengue viral infection. Patient with confirmed infection with dengue virus were prospectively evaluated for disease progression and to determine their final clinical outcome. The objective of the study was to study the clinico-haematological profile of dengue infection at rural tertiary care centre.Methods: This prospective study included 456 clinically suspected cases of dengue infection of ages more than 14 years of age. Dengue was confirmed by serological methods. Confirmed cases of dengue infection were analyzed according to age, sex and education. Clinical and hematological data were compared between dengue fever and dengue hemorrhagic fever.Results: Serologic dengue infection was positive in 220 (48%) out of 456 clinically suspected cases of the dengue infection. 127 (57.73%) patients were males and 93 (42.27%) were females. 149 (67.72%) patient had dengue fever while 71 (32.27%) had dengue hemorrhagic fever. The proportion of dengue cases was highest among male aged 15 to 24 years-old. A gradual decrease occurred in the percentage of dengue cases in the population aged 44 years-old or more. Common clinical symptoms were fever, headache, abdominal pain, myalgia, joint pain and retro-orbital pain. Common haematological abnormalities were thrombocytopenia, leukopenia and elevated alanine aminotransferase (SGOT).Conclusions: Differences in the clinical, biochemical and haematological profile of dengue virus infection, indicative of a variation in disease severity from DF to DHF. In this study, we present recent data on readily obtained clinical and laboratory data that can be used for early diagnosis and earlier appropriate treatment of dengue virus infection.

Download Full-text

TROMBOSIT TERENDAH, KENAIKAN HEMATOKRIT DAN KADAR TNF-α SEBAGAI INDIKASI KEPARAHAN INFEKSI VIRUS DENGUE PADA PASIEN DD DAN DBD

JURNAL WIDYA BIOLOGI ◽

10.32795/widyabiologi.v10i01.233 ◽

2019 ◽

Vol 10 (01) ◽

pp. 13-22

Author(s):

A.S. Siskayani ◽

I Made Sumarya ◽

N.L.P Kartika Sari

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Clinical Manifestations ◽

Hemorrhagic Fever ◽

Hemorrhagic Diathesis ◽

Dengue Virus Infection ◽

Cross Sectional ◽

Blood Samples ◽

Tnf Α ◽

The Mean

Clinical manifestations of dengue virus infection vary from unspecified fever, dengue fever (DF) and dengue hemorrhagic fever (DHF). The fundamental difference in the diagnosis between DF and DHF is plasma leakage, hypotension, thrombocytopenia and hemorrhagic diathesis in DHF. The objectives of the study were to determine the lowest platelet, haematocrit increase, and TNF-α levels in DF and DHF patients as an indication of severity of dengue virus infection. The cross-sectional study was conducted by taking 54 blood samples of patients aged 26-45 years who were infected by dengue virus and grouped into two groups: DF patient group (27 blood samples) and DHF group (27 blood samples). The lowest platelets and hematocrit rise were determined by Hematology Analyzer and the TNF-α levels were determined by ELISA. The data obtained were statistically analyzed by independent Mann-Whitney test. The results showed that the lowest mean platelet counts of DF patients were significantly (p <0.01) higher than those of DHF patients. The mean hematocrit increase in DF patients was significantly (p <0.01) lower than that of the DHF patients and the mean TNF-α levels of DF patients were significantly (p <0.01) lower than the DHF patients. Based on the results of this study it can be concluded that the lowest platelets, hematocrit increase and TNF-α levels are an indication of the severity of dengue virus infection.

Download Full-text

Sera-epidemiology of Dengue Virus Infection in Clinically Suspected Patients Attended in Dhaka Medical College Hospital During January to December 2016

Journal of Dhaka Medical College ◽

10.3329/jdmc.v26i2.38825 ◽

2018 ◽

Vol 26 (2) ◽

pp. 111-116

Author(s):

Monira Pervin ◽

Afroza Akbar Sweety ◽

Mohammad Zaid Hossain ◽

Rabeya Sharmin ◽

Nusrat Fatema ◽

...

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Dengue Fever ◽

Clinical Manifestations ◽

Medical College Hospital ◽

Dengue Virus Infection ◽

College Hospital ◽

Medical College ◽

Igm Antibody ◽

Orbital Pain

Introduction: Dengue is now established as one of the most important Arboviral infection in tropical countries. As the epidemic continues worldwide, this Aedes mosquito-transmitted pathogen is considered a major re-emerging tropical disease and significant public health concern. The study was conducted to determine the sero-epidemiology and clinical picture of dengue virus infection among the clinically suspected patients attended in Dhaka Medical College Hospital during January to December 2016.Materials and Methods: The study was carried out on 145 clinically suspected Dengue patients attended in Dhaka Medical College Hospital from January to December 2016 who had fever with temperature >39°C. Blood sample was collected during acute febrile phase of patients and separated sera were tested for NS1 dengue antigen and IgM antibodies using commercial test kits (NS1 by OMC Healthcare (Pvt.) Ltd & IgM antibody by Omega Diagnostics Ltd.).Results: Of the total 145 patients 40 (27.6%) were positive for Dengue NS1 antigen and or IgM antibody test. Of the positive 40 patients, 18 (45%) were positive for NS1, 19 (47.5%) were positive for IgM and 3 (7.5%) were positive for both NS1 and IgM antibody. Major clinical manifestations were fever with arthralgia (79.5%) and retro-orbital pain (50%).Conclusion: Outbreak of Dengue fever is continuing every year and typical presentation of Dengue fever has changed. Arthralgia and retro orbital pain was the most common clinical feature in the present study instead of headache and rash with fever. A large number of suspected dengue cases were negative by Dengue tests for antigen NS1 and IgM antibody which suggests other organisms of similar clinical manifestations are becoming prevalent in Dhaka city.J Dhaka Medical College, Vol. 26, No.2, October, 2017, Page 111-116

Download Full-text

Prevalence of dengue virus infection among population of Bhaili visiting tertiary health institution; Chhattisgarh

Asian Journal of Medical Sciences ◽

10.3126/ajms.v11i2.27345 ◽

2020 ◽

Vol 11 (2) ◽

pp. 30-34

Author(s):

Dhruba Hari Chandi

Keyword(s):

Virus Infection ◽

Early Detection ◽

Dengue Virus ◽

Dengue Fever ◽

Dengue Infection ◽

Rapid Test ◽

Age Group ◽

Immunochromatographic Test ◽

Dengue Virus Infection ◽

Health Institution

Background: Dengue fever, dengue haemorrhagic fever and dengue shock syndrome of infection is distinct clinical forms which is caused by Dengue Virus that belong to a member of the Flaviviridae family. It is most important arthropod borne viral disease that causes morbidity and mortality. Dengue virus infection is a major public health problem growing in worldwide and it is estimated about 2.5 billion people of world are at risk of this infection. In India several parts of the country it is an endemic disease. Aims and Objective: The current work is an attempt to review current perspectives of dengue infection among population of Bhilai visiting tertiary health institution. In study also we have compared the serological profiles of the dengue cases. Material and Methods: Samples received were performing retrospective analysis in the department of microbiology and process in the departmental microbiology laboratory obtaining during monsoon season. The duration of fever (in days) and other relevant clinical information were recorded from the requisition form. Rapid test for dengue as NS1Ag, IgG and IgM test were performed in accordance with the manufacturer’s instructions. The dengue NS1Ag, IgG and IgM rapid test is an in vitro immunochromatographic test (ICT) which is a onestep assay designed for the qualitative determination of dengue NS1Ag, IgG and IgM in human serum for the diagnosis dengue infection. Result: During the study period total 1308 serum samples were collected from suspected Dengue fever patients. Among 1308 samples 412(31.5%) samples were found to be positive dengue fever. In the month of August maximum number of sample were received. Out of 1308 patients with dengue suspected patients 792(60.6%) were male and 516(39.4%) were female. Among 60.6% of male 28.2% were positive and out of 39.4% of female 36.6% were positive for dengue. Age between 10-20 years old (55.83%) patients were most affected age group and 70-80 years old (8.77%) age group were least affected. Among the dengue positive patients, dengue parameter associated with thrombocytopenia was also recorded which showed that there was no significant difference between the parameters in relation to thrombocytopenia. Conclusion: Especially in developing countries like India where there are poor resources for diagnosis of dengue infection, the sensitivity of these tests is more than immunochromatographic Test (ICT). By NS1 assay early detection of dengue infection can help in early confirmation and management of this before its gets complicate. Therefore commercial available dengue NS1 antigen test kits provided additional laboratory diagnostic tool for early detection of dengue.

Download Full-text

Headache Features in Patients with Dengue Virus Infection

Cephalalgia ◽

10.1111/j.1468-2982.2006.01100.x ◽

2006 ◽

Vol 26 (7) ◽

pp. 879-882 ◽

Cited By ~ 10

Author(s):

RB Domingues ◽

GW Kuster ◽

FL Onuki de Castro ◽

VA Souza ◽

JE Levi ◽

...

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Dengue Fever ◽

Dengue Infection ◽

Severe Form ◽

Dengue Haemorrhagic Fever ◽

Neurological Involvement ◽

Haemorrhagic Fever ◽

Dengue Virus Infection

The aim of this study was to describe the frequency and features of headache among patients with confirmed dengue virus infection and to compare the headache features in patients with dengue fever and dengue haemorrhagic fever, primary and secondary dengue infection, and patients with and without neurological involvement. Patients with classic dengue fever had a more intense headache than those with the more severe form of the disease, dengue haemorrhagic fever.

Download Full-text

Enhanced passive surveillance dengue infection among febrile children: Prevalence, co-infections and associated factors in Cameroon

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009316 ◽

2021 ◽

Vol 15 (4) ◽

pp. e0009316

Author(s):

Celine Nguefeu Nkenfou ◽

Nadine Fainguem ◽

Félicitée Dongmo-Nguefack ◽

Laeticia Grace Yatchou ◽

Joel Josephine Kadji Kameni ◽

...

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Dengue Infection ◽

Hemorrhagic Fever ◽

Infection Prevalence ◽

Dengue Virus Infection ◽

Life Threatening Illness ◽

Life Threatening ◽

Triple Infection ◽

Associated Risk Factors

Dengue virus (DENV) causes a spectrum of diseases ranging from asymptomatic, mild febrile to a life-threatening illness: dengue hemorrhagic fever. The main clinical symptom of dengue is fever, similar to that of malaria. The prevalence of dengue virus infection, alone or in association with other endemic infectious diseases in children in Cameroon is unknown. The aim of this study was to determine the prevalence of dengue, malaria and HIV in children presenting with fever and associated risk factors. Dengue overall prevalence was 20.2%, Malaria cases were 52.7% and HIV cases represented 12.6%. The prevalence of dengue-HIV co-infection was 6.0% and that of Malaria-dengue co-infection was 19.5%. Triple infection prevalence was 4.3%. Dengue virus infection is present in children and HIV-Dengue or Dengue- Malaria co-infections are common. Dengue peak prevalence was between August and October. Sex and age were not associated with dengue and dengue co-infections. However, malaria as well as HIV were significantly associated with dengue (P = 0.001 and 0.028 respectively). The diagnosis of dengue and Malaria should be carried out routinely for better management of fever.

Download Full-text

Seroepidemiology of Asymptomatic Dengue Virus Infection in Jeddah, Saudi Arabia

Virology Research and Treatment ◽

10.4137/vrt.s34187 ◽

2016 ◽

Vol 7 ◽

pp. VRT.S34187 ◽

Cited By ~ 4

Author(s):

Ghazi A. Jamjoom ◽

Esam I. Azhar ◽

Moujahid A. Kao ◽

Raja M. Radadi

Keyword(s):

Saudi Arabia ◽

Virus Infection ◽

Dengue Virus ◽

Dengue Fever ◽

Blood Donors ◽

Dengue Infection ◽

Igg Antibodies ◽

Dengue Virus Infection ◽

High Exposure ◽

Dengue Viruses

Background Although virologically confirmed dengue fever has been recognized in Jeddah, Saudi Arabia, since 1994, causing yearly outbreaks, no proper seroepidemiologic studies on dengue virus have been conducted in this region. Such studies can define the extent of infection by this virus and estimate the proportion that may result in disease. The aim of this study was to measure the seroprevalence of past dengue virus infection in healthy Saudi nationals from different areas in the city of Jeddah and to investigate demographic and environmental factors that may increase exposure to infection. Methods Sera were collected from 1984 Saudi subjects attending primary health care centers in six districts of Jeddah. These included general patients of various ages seeking routine vaccinations, antenatal care or treatment of different illnesses excluding fever or suspected dengue. A number of blood donors were also tested. Serum samples were tested by enzyme immunoassay (EIA) for IgG antibodies to dengue viruses 1, 2, 3, 4. A questionnaire was completed for each patient recording various anthropometric data and factors that may indicate possible risk of exposure to mosquito bites and dengue infection. Patients with missing data and those who reported a history of dengue fever were excluded from analysis, resulting in a sample of 1939 patients to be analyzed. Results The overall prevalence of dengue virus infection as measured by anti-dengue IgG antibodies from asymptomatic residents in Jeddah was 47.8% (927/1939) and 37% (68/184) in blood donors. Infection mostly did not result in recognizable disease, as only 19 of 1956 subjects with complete information (0.1%) reported having dengue fever in the past. Anti dengue seropositivity increased with age and was higher in males than females and in residents of communal housing and multistory buildings than in villas. One of the six districts showed significant increase in exposure rate as compared to the others. Availability of public sewage was associated with lower infection at a nearly significant level. No other clear risk factors were identifiable. Infection was not related to travel abroad. Conclusions Our results indicate a relatively high exposure of Jeddah residents to infection by dengue viruses, which must be considered endemic to this region. Infection largely remained asymptomatic or was only associated with minor illness for which patients did not seek treatment. These results call for continued vigilance for clinical cases of dengue that may arise from this wide exposure. They also call for more extensive control efforts to reduce exposure to and transmission of dengue viruses.

Download Full-text