scholarly journals Genome Sequence of Lactobacillus brevis Strain D6, Isolated from Smoked Fresh Cheese

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Ravi Kant ◽  
Ksenija Uroić ◽  
Ulla Hynönen ◽  
Blaženka Kos ◽  
Jagoda Šušković ◽  
...  
Keyword(s):  
Intestinal Epithelial Cells ◽  
Starter Culture ◽  
Lactobacillus Brevis ◽  
Matrix Proteins ◽  
Intestinal Epithelial ◽  
Technological Properties ◽  
Fresh Cheese ◽  
Protein Strain ◽  
Immunomodulatory Potential ◽  
Cheese Production

The autochthonous Lactobacillus brevis strain D6, isolated from smoked fresh cheese, carries a 45-kDa S-layer protein. Strain D6 has shown adhesion to extracellular matrix proteins and to Caco-2 intestinal epithelial cells, as well as immunomodulatory potential and beneficial milk technological properties. Hence, it could be used as a potential probiotic starter culture for cheese production.

The application of kombucha inoculum as an innovative starter culture in fresh cheese production

LWT ◽  
2021 ◽  
pp. 112142
Author(s):  
Vladimir Vukić ◽  
Mirela Iličić ◽  
Dajana Vukić ◽  
Sunčica Kocić-Tanackov ◽  
Branimir Pavlić ◽  
...  
Keyword(s):  
Starter Culture ◽  
Fresh Cheese ◽  
Cheese Production

scholarly journals Lactobacillus delbrueckii CRL 581 Differentially Modulates TLR3-Triggered Antiviral Innate Immune Response in Intestinal Epithelial Cells and Macrophages

2021 ◽  
Vol 9 (12) ◽  
pp. 2449
Author(s):  
Mariano Elean ◽  
Leonardo Albarracin ◽  
Kohtaro Fukuyama ◽  
Binghui Zhou ◽  
Mikado Tomokiyo ◽  
...  
Keyword(s):  
Immune Response ◽  
Epithelial Cells ◽  
Innate Immune Response ◽  
Intestinal Epithelial Cells ◽  
Starter Culture ◽  
Innate Immune ◽  
Lactobacillus Delbrueckii ◽  
Poly I:C ◽  
Intestinal Epithelial ◽  
Antiviral Immune Response

Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture L. delbrueckii subsp. lactis CRL 581 by evaluating its interaction with intestinal epithelial cells and macrophages in the context of innate immune responses triggered by TLR3. Our results showed that the CRL 581 strain was able to adhere to porcine intestinal epithelial (PIE) cells and mucins. The CRL 581 strain also augmented the expression of antiviral factors (IFN-α, IFN-β, Mx1, OAS1, and OAS2) and reduced inflammatory cytokines in PIE cells triggered by TLR3 stimulation. In addition, the influence of L. delbrueckii subsp. lactis CRL 581 on the response of murine RAW macrophages to the activation of TLR3 was evaluated. The CRL 581 strain was capable of enhancing the expression of IFN-α, IFN-β, IFN-γ, Mx1, OAS1, TNF-α, and IL-1β. Of note, the CRL 581 strain also augmented the expression of IL-10 in macrophages. The results of this study show that the high proteolytic strain L. delbrueckii spp. lactis CRL 581 was able to beneficially modulate the intestinal innate antiviral immune response by regulating the response of both epithelial cells and macrophages relative to TLR3 activation.

The role of S-layer in adhesive and immunomodulating properties of probiotic starter culture Lactobacillus brevis D6 isolated from artisanal smoked fresh cheese

LWT ◽  
2016 ◽  
Vol 69 ◽  
pp. 623-632 ◽  
Author(s):  
Ksenija Uroić ◽  
Jasna Novak ◽  
Ulla Hynönen ◽  
Taija E. Pietilä ◽  
Andreja Leboš Pavunc ◽  
...  
Keyword(s):  
Starter Culture ◽  
Lactobacillus Brevis ◽  
Fresh Cheese

Chicken Eggshell Matrix Proteins Enhance Calcium Transport in the Human Intestinal Epithelial Cells, Caco-2

2003 ◽  
Vol 51 (20) ◽  
pp. 6056-6061 ◽  
Author(s):  
Wichittra Daengprok ◽  
Wunwiboon Garnjanagoonchorn ◽  
Onanong Naivikul ◽  
Pornpun Pornsinlpatip ◽  
Kimon Issigonis ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Calcium Transport ◽  
Intestinal Epithelial Cells ◽  
Matrix Proteins ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Chicken Eggshell ◽  
Eggshell Matrix

scholarly journals Effect of high-protein fermentation amaranth feed on the functional and technological properties of milk as raw materials for cheese production

2020 ◽  
Vol 81 (4) ◽  
pp. 166-170
Author(s):  
S. V. Pavlenkova ◽  
G. P. Shuvaeva ◽  
L. A. Miroshnichenko ◽  
T. V. Sviridova ◽  
O. S. Korneeva ◽  
...  
Keyword(s):  
Mass Fraction ◽  
Starter Culture ◽  
Raw Milk ◽  
High Protein ◽  
Winter Period ◽  
Technological Properties ◽  
Canned Food ◽  
Green Mass ◽  
Cheese Production

Improving the quality of raw milk for the production of cheese in the autumn-winter period is one of the important tasks of biotechnology. It can be solved by improving the quality of feed, as they directly affect the functional and technological properties of raw milk. Amaranth silo obtained from the green mass of amaranth by canning using the developed starter culture was used in the diet of lactating cows. Scientific and economic experience was carried out on the basis of peasant farms II Korovnikov, Khokholsky district of Voronezh region. Milk was obtained from two groups of dairy cows of the Holstein-Friesian breed with an average productivity of 4200 kg of milk, selected on the basis of analogues. The diets of the experimental and control groups in accordance with the accepted experimental scheme included canned food from the green mass of amaranth and corn. Cows were fed for 105 days and evaluated the functional and technological properties of raw milk. The results of the study of the functional and technological properties of raw milk obtained from cows, in the diet of which in the autumn-winter period included high-protein canned food from amaranth, are presented. It was found that the replacement of traditional corn silage with amaranth contributed to an increase in the most important components of raw milk for cheese production: mass fraction of protein – by 0.2% (including mass fraction of casein fraction by 0.13%) and mass fraction of fat – by 0.38%. Veterinary health indicators of raw milk have also improved. In the experimental group, there was a decrease in the number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAnM) in relation to the control by 5.6%, which correlated with a decrease in the acidity of milk.

scholarly journals Adhesive Property of Different Strains of Lactobacilli in The Presence of Resveratrol

2018 ◽  
Vol 49 (4) ◽  
pp. 291-296 ◽  
Author(s):  
V. Jarosova ◽  
I. Doskocil ◽  
T. Volstatova ◽  
J. Havlik
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Biological Effects ◽  
Lactobacillus Brevis ◽  
Bacterial Suspension ◽  
Cytotoxicity Test ◽  
Intestinal Epithelial ◽  
Dietary Polyphenols ◽  
Adhesion Ability

Abstract The ability of bacteria to adhere to the intestinal epithelial cells is one of the main criteria for selection of new probiotic strains. Some dietary polyphenols have been proven to affect bacterial adhesion, providing a rationale for the use of mixtures of polyphenols and probiotics. Resveratrol, a naturally occurring stilbene in plants, has been shown to have a number of beneficial biological effects. The adhesion ability of four Lactobacillus strains (Lactobacillus brevis, L. fermentum, L. gasseri, and L. plantarum), in the presence of resveratrol, has been investigated in an in vitro model based on mixed co-culture of Caco-2 and HT29-MTX intestinal epithelial cells. The effective concentration of resveratrol used in the adhesion experiment has been selected based on cytotoxicity test. Resveratrol at three physiologically low concentrations (4.5, 2.25, and 1.125 µg ml−1), added together with the bacterial suspension, had no statistically significant influence on the adhesion of any strain (P < 0.05). Since the health benefits of polyphenols are often associated with the composition of gut microbiota, the knowledge of interactions between known bacteria and polyphenols would be of high scientific value.

Membrane-bound vesicles associated with the microvilli in the midgut of the freshwater microcrustacean, Daphnia magna

1983 ◽  
Vol 41 ◽  
pp. 480-481
Author(s):  
Patricia L. Jansma
Keyword(s):  
Daphnia Magna ◽  
Intestinal Epithelial Cells ◽  
Uranyl Acetate ◽  
Intestinal Epithelial ◽  
Chlamydomonas Reinhardii ◽  
Thin Sections ◽  
Saturated Water ◽  
Cacodylate Buffer ◽  
Membrane Bound ◽  
Ethanol Series

The presence of the membrane bound vesicles or blebs on the intestinal epithelial cells has been demonstrated in a variety of vertebrates such as chicks, piglets, hamsters, and humans. The only invertebrates shown to have these microvillar blebs are two species of f1ies. While investigating the digestive processes of the freshwater microcrustacean, Daphnia magna, the presence of these microvillar blebs was noticed.Daphnia magna fed in a suspension of axenically grown green alga, Chlamydomonas reinhardii for one hour were narcotized with CO2 saturated water. The intestinal tracts were excised in 2% glutaraldehyde in 0.2 M cacodyl ate buffer and then placed in fresh 2% glutaraldehyde for one hour. After rinsing in 0.1 M cacodylate buffer, the sample was postfixed in 2% OsO4, dehydrated with a graded ethanol series, infiltrated and embedded with Epon-Araldite. Thin sections were stained with uranyl acetate and Reynolds lead citrate before viewing with the Philips EM 200.

Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

1992 ◽  
Vol 50 (1) ◽  
pp. 576-577
Author(s):  
Julian P. Heath ◽  
Buford L. Nichols ◽  
László G. Kömüves
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Cell Surfaces ◽  
Basal Surface ◽  
Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

Sign in / Sign up

Export Citation Format

Share Document