scholarly journals Adhesive Property of Different Strains of Lactobacilli in The Presence of Resveratrol

2018 ◽  
Vol 49 (4) ◽  
pp. 291-296 ◽  
Author(s):  
V. Jarosova ◽  
I. Doskocil ◽  
T. Volstatova ◽  
J. Havlik
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Biological Effects ◽  
Lactobacillus Brevis ◽  
Bacterial Suspension ◽  
Cytotoxicity Test ◽  
Intestinal Epithelial ◽  
Dietary Polyphenols ◽  
Adhesion Ability

Abstract The ability of bacteria to adhere to the intestinal epithelial cells is one of the main criteria for selection of new probiotic strains. Some dietary polyphenols have been proven to affect bacterial adhesion, providing a rationale for the use of mixtures of polyphenols and probiotics. Resveratrol, a naturally occurring stilbene in plants, has been shown to have a number of beneficial biological effects. The adhesion ability of four Lactobacillus strains (Lactobacillus brevis, L. fermentum, L. gasseri, and L. plantarum), in the presence of resveratrol, has been investigated in an in vitro model based on mixed co-culture of Caco-2 and HT29-MTX intestinal epithelial cells. The effective concentration of resveratrol used in the adhesion experiment has been selected based on cytotoxicity test. Resveratrol at three physiologically low concentrations (4.5, 2.25, and 1.125 µg ml−1), added together with the bacterial suspension, had no statistically significant influence on the adhesion of any strain (P < 0.05). Since the health benefits of polyphenols are often associated with the composition of gut microbiota, the knowledge of interactions between known bacteria and polyphenols would be of high scientific value.

Effect of Cellulase Genes Recombinant L. reuteri on Digestibility of Diet Crude Fiber and Adhesion in Gut of Chicken

2020 ◽  
Author(s):  
Wang Li ◽  
Jingbo Zhang ◽  
Peng Cheng ◽  
Longmei Zhao
Keyword(s):  
Epithelial Cells ◽  
Digestive Tract ◽  
Intestinal Epithelial Cells ◽  
Fermentation Broth ◽  
Crude Fiber ◽  
Control Group ◽  
Intestinal Epithelial ◽  
Adhesion Ability ◽  
And Control

Background: The aim of this test was study the gut adhesion ability of recombinant Lactobacilli and their improvement of the digestibility of crude fiber in chick diet. Methods: The adhesion ability was observed through in vitro and in vivo tests. Intestinal epithelial cells and recombinant Lactobacillus were co-cultured for observation of adhesion. One hundred one-day-old chicks were randomly divided into four groups; C15, C73, C1573 and control. Each chick in C15, C73, C1573 and control were orally gavaged with 200 ìL of fermentation broth of recombinant L. reuteri XNY-Cel15, 200 ìL of fermentation broth of recombinant L. reuteri XNY-Cel73 and 200 ìL of broth containing equal XNY-Cel15 and XNY-Cel73, 200 ìL of MRS broth, once at the beginning of the trial respectively. Result: Recombinant L. reuteri adhered to chick intestinal epithelial cells which co-cultured together in vitro. After feeding for 30 days, the reporter genes of recombinant Lactobacillus was detected in the crop, small intestine and cecum of trial chicks, with their sequences corresponding to those of the insert genes. The digestibility of crude fiber in experimental groups was higher than that in control group and the digestibility of C15 was significantly higher than other groups (P less than 0.01). It showed that the recombinant Lactobacillus can survive in the digestive tract of chicken for more than 30 days and helped to decompose the crude fiber in the digestive tract.

scholarly journals Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 328 ◽  
Author(s):  
Claudio Salaris ◽  
Melania Scarpa ◽  
Marina Elli ◽  
Alice Bertolini ◽  
Simone Guglielmetti ◽  
...  
Keyword(s):  
Immune Response ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Plaque Assay ◽  
Immune Response Gene ◽  
Intestinal Epithelial ◽  
Antiviral Immune Response ◽  
Qrt Pcr ◽  
Anti Inflammatory

SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.

The effect of berberine in vitro on tight junctions in human Caco-2 intestinal epithelial cells

Fitoterapia ◽  
2009 ◽  
Vol 80 (4) ◽  
pp. 241-248 ◽  
Author(s):  
Lili Gu ◽  
Ning Li ◽  
Qiurong Li ◽  
Qiang Zhang ◽  
Chengyang Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

scholarly journals A σ28-Regulated Nonflagella Gene Contributes to Virulence of Campylobacter jejuni 81-176

2006 ◽  
Vol 74 (1) ◽  
pp. 769-772 ◽  
Author(s):  
Scarlett Goon ◽  
Cheryl P. Ewing ◽  
Maria Lorenzo ◽  
Dawn Pattarini ◽  
Gary Majam ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Diarrheal Disease ◽  
Intestinal Epithelial Cells ◽  
Disease Model ◽  
Intestinal Epithelial ◽  
Model Expression

ABSTRACT A Campylobacter jejuni 81-176 mutant in Cj0977 was fully motile but reduced >3 logs compared to the parent in invasion of intestinal epithelial cells in vitro. The mutant was also attenuated in a ferret diarrheal disease model. Expression of Cj0977 protein was dependent on a minimal flagella structure.

Proteomic response of inflammatory stimulated intestinal epithelial cells to in vitro digested plums and cabbages rich in carotenoids and polyphenols

2016 ◽  
Vol 7 (10) ◽  
pp. 4388-4399 ◽  
Author(s):  
Anouk Kaulmann ◽  
Sébastien Planchon ◽  
Jenny Renaut ◽  
Yves-Jacques Schneider ◽  
Lucien Hoffmann ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Bowel Diseases ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Proteomic Response

Proteomic response of intestinal cells as a model of inflammatory bowel diseases to digested plum and cabbage rich in polyphenols and carotenoids.

Progastrin1–80stimulates growth of intestinal epithelial cells in vitro via high-affinity binding sites

2003 ◽  
Vol 284 (2) ◽  
pp. G328-G339 ◽  
Author(s):  
P. Singh ◽  
X. Lu ◽  
S. Cobb ◽  
B. T. Miller ◽  
N. Tarasova ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Binding Sites ◽  
Intestinal Epithelial Cells ◽  
Full Length ◽  
Intestinal Epithelial ◽  
Growth Effects ◽  
High Affinity ◽  
Significant Difference

Proliferation and carcinogenesis of the large intestinal epithelial cells (IEC) cells is significantly increased in transgenic mice that overexpress the precursor progastrin (PG) peptide. It is not known if the in vivo growth effects of PG on IEC cells are mediated directly or indirectly. Full-length recombinant human PG (rhPG1–80) was generated to examine possible direct effects of PG on IEC cells. Surprisingly, rhPG (0.1–1.0 nM) was more effective than the completely processed gastrin 17 (G17) peptide as a growth factor. Even though IEC cells did not express CCK1and CCK2receptors (-R), fluorescently labeled G17 and Gly-extended G17 (G-Gly) were specifically bound to the cells, suggesting the presence of binding proteins other than CCK1-R and CCK2-R on IEC cells. High-affinity ( Kd= 0.5–1.0 nM) binding sites for125I-rhPG were discovered on IEC cells that demonstrated relative binding affinity for gastrin-like peptides in the order PG ≥ COOH-terminally extended G17 ≥ G-Gly > G17 > *CCK-8 (* significant difference; P< 0.05). In conclusion, our studies demonstrate for the first time direct growth effects of the full-length precursor peptide on IEC cells in vitro that are apparently mediated by the high-affinity PG binding sites that were discovered on these cells.

A thermal injury-induced circulating factor(s) compromises intestinal cell morphology, proliferation, and migration

1999 ◽  
Vol 277 (1) ◽  
pp. G175-G182 ◽  
Author(s):  
Maryam Varedi ◽  
George H. Greeley ◽  
David N. Herndon ◽  
Ella W. Englander
Keyword(s):  
Epithelial Cells ◽  
Thermal Injury ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Rat Serum ◽  
In Vitro Effects ◽  
Mucosal Morphology ◽  
And Migration

The effects of a 60% body surface area thermal injury in rats on the morphology and proliferation of the epithelium of the small intestine and the in vitro effects of serum collected from scalded rats on intestinal epithelial cells were investigated. Scald injury caused significant reductions in duodenal villus width and crypt dimensions, villus enterocytes changed in shape from columnar to cuboidal, and the number of goblet cells decreased. The proportion of bromodeoxyuridine-labeled S phase cells in crypts was also diminished. In vitro, incubation of intestinal epithelial cells (IEC-6) with scalded rat serum (SRS) collected at either 12 or 24 h after injury caused a disruption in the integrity of the confluent culture and induced the appearance of large denuded areas. SRS also decreased DNA synthesis and delayed wound closure in an in vitro wound-healing model. The thermal injury-induced changes in intestinal mucosal morphology and epithelial cell growth characteristics described in this study may underlie, in part, the mechanism(s) involved in the diminished absorption of nutrients, increased intestinal permeability, and sepsis in patients with thermal injury.

Sign in / Sign up

Export Citation Format

Share Document