scholarly journals Genome Sequences of Escherichia coli Strains That Cause Persistent and Transient Mastitis

2017 ◽  
Vol 5 (34) ◽  
Author(s):  
Tyler C. Thacker ◽  
John D. Lippolis ◽  
Brian W. Brunelle ◽  
Thomas A. Casey ◽  
Timothy A. Reinhardt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Persistent Infection ◽  
Bovine Mastitis ◽  
Genome Sequences ◽  
Content Type

ABSTRACT We report here the genome sequences of two strains of Escherichia coli (ECA-B and ECC-M) that cause bovine mastitis. These strains are known to be associated with persistent and transient mastitis; strain ECA-B causes a transient infection, and ECC-M leads to a persistent infection.

scholarly journals Draft Genome Sequences of 113 Escherichia coli Strains Isolated from Intramammary Infections in Dairy Cattle

2021 ◽  
Vol 10 (7) ◽  
Author(s):  
Dongyun Jung ◽  
Soyoun Park ◽  
Janina Ruffini ◽  
Simon Dufour ◽  
Jennifer Ronholm
Keyword(s):  
Escherichia Coli ◽  
Dairy Cattle ◽  
Draft Genome ◽  
Bovine Mastitis ◽  
Holstein Cows ◽  
Genome Sequences ◽  
Content Type ◽  
Intramammary Infections ◽  
E Coli ◽  
Etiological Agents

ABSTRACT Escherichia coli is one of the most common etiological agents responsible for clinical bovine mastitis. Here, we report the draft genome sequences and annotations of 113 E. coli strains that were isolated from Holstein cows with intramammary infections in Canada.

scholarly journals Genomic and Transcriptomic Analysis of Escherichia coli Strains Associated with Persistent and Transient Bovine Mastitis and the Role of Colanic Acid

2017 ◽  
Vol 86 (1) ◽  
Author(s):  
John D. Lippolis ◽  
Devin B. Holman ◽  
Brian W. Brunelle ◽  
Tyler C. Thacker ◽  
Bradley L. Bearson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Cattle ◽  
Rna Sequencing ◽  
Persistent Infection ◽  
Bovine Mastitis ◽  
Content Type ◽  
E Coli ◽  
Persistent Infections ◽  
Colanic Acid

ABSTRACTEscherichia coliis a leading cause of bacterial mastitis in dairy cattle. It is most often transient in nature, causing an infection that lasts 2 to 3 days. However,E. colihas been shown to cause a persistent infection in a minority of cases. Mechanisms that allow for a persistentE. coliinfection are not fully understood. The goal of this work was to determine differences betweenE. colistrains originally isolated from dairy cattle with transient and persistent mastitis. Using RNA sequencing, we show gene expression differences in nearly 200 genes when bacteria from the two clinical phenotypes are compared. We sequenced the genomes of theE. colistrains and report genes unique to the two phenotypes. Differences in thewcaoperon, which encodes colanic acid, were identified by DNA as well as RNA sequencing and differentiated the two phenotypes. Previous work demonstrated thatE. colistrains that cause persistent infections were more motile than those that cause transient infections. Deletion of genes in thewcaoperon from a persistent-infection strain resulted in a reduction of motility as measured in swimming and swarming assays. Furthermore, colanic acid has been shown to protect bacteria from complement-mediated killing. We show that transient-infectionE. colistrains were more sensitive to complement-mediated killing. The deletion of genes from thewcaoperon caused a persistent-infectionE. colistrain to become sensitive to complement-mediated killing. This work identifies important differences betweenE. colistrains that cause persistent and transient mammary infections in dairy cattle.

scholarly journals Draft Genome Sequences of Escherichia coli Strains FP2 and FP3, Isolated from the Canada Goose (Branta canadensis)

2018 ◽  
Vol 7 (9) ◽  
Author(s):  
Allison L. Denny ◽  
Susan E. Arruda
Keyword(s):  
Escherichia Coli ◽  
Draft Genome ◽  
Branta Canadensis ◽  
Canada Goose ◽  
Genome Sequences ◽  
Content Type

Draft genomes of two strains of Escherichia coli, FP2 and FP3, isolated from the feces of the Canada goose (Branta canadensis), were sequenced. Genome sizes were 5.26 Mb with a predicted G+C content of 50.54% (FP2) and 5.07 Mb with a predicted G+C content of 50.41% (FP3).

scholarly journals Species-Wide Collection of Escherichia coli Isolates for Examination of Genomic Diversity

2017 ◽  
Vol 5 (50) ◽  
Author(s):  
Jayanthi Gangiredla ◽  
Mark K. Mammel ◽  
Tammy J. Barnaba ◽  
Carmen Tartera ◽  
Solomon T. Gebru ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Health ◽  
Potential Risk ◽  
Phylogenetic Relationships ◽  
Genomic Diversity ◽  
Genome Sequences ◽  
Content Type ◽  
E Coli

ABSTRACT Pathogenic and nonpathogenic Escherichia coli strains present a vast genomic diversity. We report the genome sequences of 2,244 E. coli isolates from multiple animal and environmental sources. Their phylogenetic relationships and potential risk to human health were examined.

scholarly journals Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Håkon Kaspersen ◽  
Thomas H. A. Haverkamp ◽  
Hanna Karin Ilag ◽  
Øivind Øines ◽  
Camilla Sekse ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Complete Genome ◽  
Flow Cell ◽  
Hybrid Assembly ◽  
Genome Sequences ◽  
Content Type ◽  
Short Reads ◽  
Long Reads ◽  
Long Read ◽  
Hybrid Assemblies

ABSTRACT In total, 12 quinolone-resistant Escherichia coli (QREC) strains containing qnrS1 were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly.

scholarly journals Complete Genome Sequences of Seven Uropathogenic Escherichia coli Strains Isolated from Postmenopausal Women with Recurrent Urinary Tract Infection

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Belle M. Sharon ◽  
Amber Nguyen ◽  
Amanda P. Arute ◽  
Neha V. Hulyalkar ◽  
Vivian H. Nguyen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Postmenopausal Women ◽  
Complete Genome ◽  
Recurrent Urinary Tract Infection ◽  
Uropathogenic Escherichia Coli ◽  
Genome Sequences ◽  
Content Type

ABSTRACT Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infection (UTI). This disease disproportionately affects women and frequently develops into recurrent UTI (rUTI) in postmenopausal women. Here, we report the complete genome sequences of seven UPEC isolates obtained from the urine of postmenopausal women with rUTI.

scholarly journals Draft Genome Sequences of 27 Staphylococcus aureus Strains and 3 Staphylococcus Species Strains Isolated from Bovine Intramammary Infections

2020 ◽  
Vol 9 (19) ◽  
Author(s):  
Soyoun Park ◽  
Dongyun Jung ◽  
Simon Dufour ◽  
Jennifer Ronholm
Keyword(s):  
Staphylococcus Aureus ◽  
Draft Genome ◽  
Bovine Mastitis ◽  
Holstein Cows ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Intramammary Infections ◽  
Intramammary Infection ◽  
Etiological Agents

Staphylococcus aureus is one of the most common etiological agents responsible for contagious bovine mastitis. Here, we report the draft whole-genome sequences, with annotations, of 27 S. aureus strains and 3 Staphylococcus species strains that were isolated from Holstein cows with intramammary infection in Canada.

scholarly journals Draft Genome Sequences of Colistin-Resistant MCR-1-Producing Escherichia coli ST1850 and ST74 Strains Isolated from Commercial Chicken Meat

2017 ◽  
Vol 5 (20) ◽  
Author(s):  
Daniel F. Monte ◽  
Miriam R. Fernandes ◽  
Louise Cerdeira ◽  
Tiago A. de Souza ◽  
Andressa Mem ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Draft Genome ◽  
Chicken Meat ◽  
Sequence Type ◽  
Genome Sequences ◽  
Content Type ◽  
Commercial Chicken

ABSTRACT We present here the draft genome sequences of two colistin-resistant mcr-1-carrying Escherichia coli strains belonging to sequence type 74 (ST74) and ST1850, isolated from commercial chicken meat in Brazil. Assembly of this draft genome resulted in 5,022,083 and 4,950,681 bp, respectively, revealing the presence of the IncX4 plasmid-mediated mcr-1 gene responsible for resistance to colistin.

scholarly journals High-Quality Whole-Genome Sequences for 77 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing

2018 ◽  
Vol 6 (19) ◽  
Author(s):  
Pooja N. Patel ◽  
Rebecca L. Lindsey ◽  
Lisley Garcia-Toledo ◽  
Lori A. Rowe ◽  
Dhwani Batra ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Foodborne Pathogen ◽  
Severe Illness ◽  
Whole Genome ◽  
Genome Sequences ◽  
Pacbio Sequencing ◽  
High Quality ◽  
Content Type ◽  
Sequencing Platform

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) is an enteric foodborne pathogen that can cause mild to severe illness. Here, we report the availability of high-quality whole-genome sequences for 77 STEC strains generated using the PacBio sequencing platform.

scholarly journals Draft Genome Sequences of Five Shiga Toxin-Producing Escherichia coli Isolates Harboring the New and Recently Described Subtilase Cytotoxin Allelic Variant subAB2-3

2017 ◽  
Vol 5 (8) ◽  
Author(s):  
Taurai Tasara ◽  
Lisa Fierz ◽  
Jochen Klumpp ◽  
Herbert Schmidt ◽  
Roger Stephan
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Sequence Data ◽  
Draft Genome ◽  
Allelic Variant ◽  
Genome Sequences ◽  
Content Type ◽  
Subtilase Cytotoxin

ABSTRACT We present here the draft genome sequences of five Shiga toxin-producing Escherichia coli (STEC) strains which tested positive in a primary subAB screening. Assembly and annotation of the draft genomes revealed that all strains harbored the recently described allelic variant subAB 2-3 . Based on the sequence data, primers were designed to identify and differentiate this variant.

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