scholarly journals The eutD Gene of Salmonella enterica Encodes a Protein with Phosphotransacetylase Enzyme Activity

2004 ◽  
Vol 186 (6) ◽  
pp. 1890-1892 ◽  
Author(s):  
Shaun R. Brinsmade ◽  
Jorge C. Escalante-Semerena
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Enzyme Activity ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Salmonella Enterica ◽  
Coenzyme A ◽  
Catalytic Domain ◽  
Activity Level ◽  
Acetyl Coenzyme A

ABSTRACT The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the product of the reaction. The EutD protein restored growth of an S. enterica pta strain on acetate as the source of carbon and energy.

scholarly journals Fast log P determination by ultra-high-pressure liquid chromatography coupled with UV and mass spectrometry detections

2009 ◽  
Vol 394 (7) ◽  
pp. 1919-1930 ◽  
Author(s):  
Yveline Henchoz ◽  
Davy Guillarme ◽  
Sophie Martel ◽  
Serge Rudaz ◽  
Jean-Luc Veuthey ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Log P ◽  
Ultra High Pressure
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