Distribution of Non-Locus of Enterocyte Effacement Pathogenic Island-Related Genes in Escherichia coli carrying eae from Patients with Diarrhea and Healthy Individuals in Japan

The purpose of this work was to study the features of the clinical course of dysentery in patients with specific and nonspecific bacterial sensitization. There were 103 patients aged from 17 to 64 years under observation: 76 patients with dysentery and 27 with influenza. Fifteen healthy individuals were examined as controls.

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Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

Journal of Medical Microbiology ◽

10.1099/jmm.0.000468 ◽

2017 ◽

Vol 66 (5) ◽

pp. 577-583 ◽

Cited By ~ 14

Author(s):

Clarisse Oliveira-Pinto ◽

Cristiane Diamantino ◽

Patrícia L Oliveira ◽

Mariana P Reis ◽

Patrícia S Costa ◽

...

Keyword(s):

Escherichia Coli ◽

Urinary Infection ◽

Healthy Individuals ◽

Class 1 Integrons ◽

Class 1

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EDL933 Strains of Escherichia coli O157 can Demonstrate Genetic Diversity and Differential Adherence to Bovine Recto-Anal Junction Squamous Epithelial Cells

The Open Microbiology Journal ◽

10.2174/1874285802115010129 ◽

2021 ◽

Vol 15 (1) ◽

pp. 129-138

Author(s):

Raegan S. Hoefler ◽

Indira T. Kudva

Keyword(s):

Escherichia Coli ◽

Wild Type ◽

Cell Adherence ◽

O157 Strain ◽

Shiga Toxins ◽

The Us ◽

Strain Type ◽

Enterocyte Effacement ◽

Different Sources

Background: Differences between Escherichia coli O157 (O157) strains are well-established with some of these strains being associated with major outbreaks in the US. EDL933 is one such O157 strain that caused a multistate outbreak in 1982 and has since been used as a prototype in various O157-related experiments. Objective: As O157 can readily acquire genetic mutations, we sought to determine if the genetic and phenotypic profiles of EDL933 strains from different sources would be consistent. Methods: We evaluated wild-type O157 strains stocked as EDL933 from three different laboratories, in the strain typing Polymorphic Amplified Typing Sequence (PATS) and the bovine rectal-anal junction squamous epithelial (RSE) cell- and HEp-2 cell- adherence assays. In addition, we also verified if Shiga toxins (Stx), the Locus of Enterocyte Effacement (LEE) or curli fimbriae contributed to the adherence phenotypes observed using mutant and wild-type EDL933 isolates. Results: Our results showed differences in PATS profiles and RSE cell-adherence phenotype, with no influence from the Stx or LEE genes, between EDL933 from different sources. Interestingly, the EDL933 strain that demonstrated the most contrasting diffuse adherence phenotype on RSE cells, EDL933-T, had decreased curli production that may have contributed to this phenotype. Conclusion: Our observations suggest that a comprehensive characterization of bacterial isolates, even if assigned to the same strain type prior to use in experiments, is warranted to ensure consistency and reproducibility of results.

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Prevalence Of Extended Spectrum Beta-Lactamases (ESBLs)-Producing Escherichia Coli Isolated From UTI Patients Attending some Selected Hospitals In Minna, Nigeria

Nigerian Journal of Biotechnology ◽

10.4314/njb.v37i2.6 ◽

2021 ◽

Vol 37 (2) ◽

pp. 56-73

Author(s):

F Iseghohi ◽

J.C Igwe ◽

M Galadima ◽

A.F Kuta ◽

A.M Abdullahi ◽

...

Keyword(s):

Escherichia Coli ◽

Multidrug Resistance ◽

Urine Samples ◽

Antibiotics Resistance ◽

Healthy Individuals ◽

Beta Lactamases ◽

E Coli ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases ◽

Tract Infections

Globally, urinary tract infections are one of the most common infections in need of urgent clinical attention. The prevalence of extended spectrum beta-lactamases (ESBL)- producing Escherichia coli isolated from urine samples of some UTI patients and s of apparently healthy individuals in Minna, Nigeria, is investigated. Standard microbiological techniques were used to conduct this study. A total of 170 catch midstream urine samples submitted to the Medical Microbiology Laboratories of 4 different hospitals (and samples from healthy individuals) were randomly collected for 5 months and examined for microbial growths. Female patients (65.9%) submitted more urine samples for UTI test than their male counterpart (34.1%). The age ranges of 21 -30 (26.5%) and 31 - 40 (25.3%) had the highest percentages of infection rate while those within the ages 1- 10 (3.5%) and ≥ 71 (2.3%) were the least infected. This study observed a prevalence of 23.5% of E. coli in Minna metropolis and a significant number (30%) of healthy individuals (HI) was observed to harbor the E. coli in their urine. The isolates were highly susceptible to Gentamicin (65%), Ofloxacin (65%), Tetracycline (62.5%), Cotrimoxazole (62.5%), and Streptomycin (57.5%). Mildly susceptible to Pefloxacin (37.5%), Chloramphenicol (37.5%), and Ciprofloxacin (35%). There were significant resistance to most of the beta-lactames tested [Cefuroxime (80%), Amoxicillin (42.5%), Augmentin (40), Cefotaxime (20%) and Ceftaxidime (7.5%)]. Two of the isolates were resistant to all the 13 antibiotics tested; 70% (28) of the isolates had multiple antibiotics resistance index (MARI) ≥0.3. Multidrug resistance was expressed in 37.5% of the isolates tested. The study showed a vast resistant pool in the environment. Only 25% of the E. coli isolated from the urine samples produced beta-lactamases phenotypically, most of which expressed resistance to more than 5 of the antibiotics tested and had MARI of ≥ 0.5. Further evaluation showed that 25% (10/40) of the E. coli isolated from the UTI patients in Minna, Nigeria, were ESBL- producers and could harbor one or two of the genes. TEM gene was expressed in 70% (7) of the isolates that produced ESBL phenotypically, 60% 6) harbored CTXM gene, 20% (2) had the OXA gene while none of the bacteria harbored the SHV gene. The study established a 5.9% ESBL prevalence among the E. coli isolated from UTI in the environment studied. This study established that E. coli is one of the prevalent bacteri urea majorly isolated from UTI patients in Minna. The prevalent E. coli are multidrug resistant and could harbor more than one ESBL gene . keywords: Escherichia coli, Minna, UTI, ESBL, Multidrug resistance

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LrhA positively controls the expression of the locus of enterocyte effacement genes in enterohemorrhagic Escherichia coli by differential regulation of their master regulators PchA and PchB

Molecular Microbiology ◽

10.1111/j.1365-2958.2009.06937.x ◽

2009 ◽

Vol 74 (6) ◽

pp. 1393-1341 ◽

Cited By ~ 19

Author(s):

Naoko Honda ◽

Sunao Iyoda ◽

Shouji Yamamoto ◽

Jun Terajima ◽

Haruo Watanabe

Keyword(s):

Escherichia Coli ◽

Differential Regulation ◽

Enterohemorrhagic Escherichia Coli ◽

Master Regulators ◽

Enterocyte Effacement

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Outbreak of Shiga Toxin–Producing Escherichia coli (STEC) O104:H4 Infection in Germany Causes a Paradigm Shift with Regard to Human Pathogenicity of STEC Strains

Journal of Food Protection ◽

10.4315/0362-028x.jfp-11-452 ◽

2012 ◽

Vol 75 (2) ◽

pp. 408-418 ◽

Cited By ~ 151

Author(s):

LOTHAR BEUTIN ◽

ANNETT MARTIN

Keyword(s):

Escherichia Coli ◽

Hemolytic Uremic Syndrome ◽

Shiga Toxin ◽

Outbreak Strain ◽

Fenugreek Seeds ◽

E Coli ◽

Aggregative Adherence ◽

Uremic Syndrome ◽

Enterocyte Effacement ◽

The Way

An outbreak that comprised 3,842 cases of human infections with enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104:H4 occurred in Germany in May 2011. The high proportion of adults affected in this outbreak and the unusually high number of patients that developed hemolytic uremic syndrome makes this outbreak the most dramatic since enterohemorrhagic E. coli (EHEC) strains were first identified as agents of human disease. The characteristics of the outbreak strain, the way it spread among humans, and the clinical signs resulting from EAHEC infections have changed the way Shiga toxin–producing E. coli strains are regarded as human pathogens in general. EAHEC O104:H4 is an emerging E. coli pathotype that is endemic in Central Africa and has spread to Europe and Asia. EAHEC strains have evolved from enteroaggregative E. coli by uptake of a Shiga toxin 2a (Stx2a)–encoding bacteriophage. Except for Stx2a, no other EHEC-specific virulence markers including the locus of enterocyte effacement are present in EAHEC strains. EAHEC O104:H4 colonizes humans through aggregative adherence fimbrial pili encoded by the enteroaggregative E. coli plasmid. The aggregative adherence fimbrial colonization mechanism substitutes for the locus of enterocyte effacement functions for bacterial adherence and delivery of Stx2a into the human intestine, resulting clinically in hemolytic uremic syndrome. Humans are the only known natural reservoir known for EAHEC. In contrast, Shiga toxin–producing E. coli and EHEC are associated with animals as natural hosts. Contaminated sprouted fenugreek seeds were suspected as the primary vehicle of transmission of the EAHEC O104:H4 outbreak strain in Germany. During the outbreak, secondary transmission (human to human and human to food) was important. Epidemiological investigations revealed fenugreek seeds as the source of entry of EAHEC O104:H4 into the food chain; however, microbiological analysis of seeds for this pathogen produced negative results. The survival of EAHEC in seeds and the frequency of human carriers of EAHEC should be investigated for a better understanding of EAHEC transmission routes.

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Mutational Analysis of the Locus of Enterocyte Effacement-Encoded Regulator (Ler) of Enteropathogenic Escherichia coli

Journal of Bacteriology ◽

10.1128/jb.00663-08 ◽

2008 ◽

Vol 190 (23) ◽

pp. 7808-7818 ◽

Cited By ~ 23

Author(s):

Gal Yerushalmi ◽

Chen Nadler ◽

Tatiana Berdichevski ◽

Ilan Rosenshine

Keyword(s):

Escherichia Coli ◽

Type Iii Secretion ◽

Mutational Analysis ◽

Bacterial Colonization ◽

Point Mutations ◽

Dominant Negative ◽

Compensatory Mechanism ◽

Dependent Manner ◽

Enterocyte Effacement ◽

Related Proteins

ABSTRACT The locus of enterocyte effacement (LEE) pathogenicity island of enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC, respectively) comprises a cluster of operons encoding a type III secretion system and related proteins, all of which are essential for bacterial colonization of the host intestines. The LEE1 operon encodes Ler, which positively regulates many EPEC and EHEC virulence genes located in the LEE region and elsewhere in the chromosome. In addition, Ler is a specific autorepressor of LEE1 transcription. To better understand the function of Ler, we screened for Ler mutants defective in autorepression. We isolated 18 different point mutations in Ler, rendering it defective in autorepression and in DNA binding. Among these mutants were those defective in positive regulation as well as in autorepression, dominant-negative mutants, and a mutant deficient in oligomerization. Importantly, a group of Ler autorepression mutants complemented an EPEC ler deletion mutant for transcription activation in a dosage-dependent manner, suggesting that Ler and possibly other autorepressors have an intrinsic compensatory mechanism that enables them to sustain mutations. In addition, the phenotypes of the different mutants identified by the screen define a novel domain in Ler that is required for oligomerization.

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Toxins of Locus of Enterocyte Effacement-Negative Shiga Toxin-Producing Escherichia coli

Toxins ◽

10.3390/toxins10060241 ◽

2018 ◽

Vol 10 (6) ◽

pp. 241 ◽

Cited By ~ 9

Author(s):

Maike Krause ◽

Holger Barth ◽

Herbert Schmidt

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Enterocyte Effacement

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Whole-Genome Analysis of a Shiga Toxin-Producing Escherichia coli O103:H2 Strain Isolated from Cattle Feces

Microbiology Resource Announcements ◽

10.1128/mra.00896-20 ◽

2020 ◽

Vol 9 (45) ◽

Author(s):

Yujie Zhang ◽

Yen-Te Liao ◽

Vivian C. H. Wu

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Whole Genome Sequence ◽

Whole Genome ◽

Whole Genome Analysis ◽

Content Type ◽

Cattle Feces ◽

Beef Products ◽

Foodborne Outbreaks ◽

Enterocyte Effacement

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) serotype O103 is one of the primary pathogenic contaminants of beef products, contributing to several foodborne outbreaks in recent years. Here, we report the whole-genome sequence of a STEC O103:H2 strain isolated from cattle feces that contains a locus of enterocyte effacement (LEE) pathogenicity island.

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