scholarly journals Molecular Characterization of Mycoplasma pneumoniae Isolates in the United States from 2012 to 2018

2020 ◽  
Vol 58 (10) ◽  
Author(s):  
L. Xiao ◽  
A. E. Ratliff ◽  
D. M. Crabb ◽  
E. Mixon ◽  
X. Qin ◽  
...  
Keyword(s):  
United States ◽  
Mycoplasma Pneumoniae ◽  
Tandem Repeats ◽  
Geographic Location ◽  
The United States ◽  
Resistance Rate ◽  
Macrolide Resistance ◽  
Content Type ◽  
Mlva Type ◽  
Epidemiological Characteristics

ABSTRACT Mycoplasma pneumoniae is a major cause of community-acquired pneumonia. There are limited data in the United States on the molecular epidemiological characteristics of M. pneumoniae. We collected 446 M. pneumoniae-positive specimens from 9 states between August 2012 and October 2018. Culture, antimicrobial susceptibility testing, P1 subtyping, and multilocus VNTR (variable-number tandem repeats) analysis (MLVA) were performed to characterize the isolates. Macrolide-resistant M. pneumoniae (MRMp) was detected in 37 (8.3%) specimens. P1 subtype 2 (P1-2) was the predominant P1 subtype (59.8%). P1 subtype distribution did not change significantly chronologically or geographically. The macrolide resistance rate in P1 subtype 1 (P1-1) samples was significantly higher than that in P1-2 (12.9% versus 5.5%). Six P1-2 variants were identified, including two novel types, and variant 2c was predominant (64.6%). P1-2 variants were distributed significantly differently among geographic regions. Classical P1-2 was more frequent in lower respiratory tract specimens and had longer p1 trinucleotide repeats. Classical P1-2 was most common in MRMp (35.7%), while variant 2c was most common in macrolide-susceptible M. pneumoniae (67.5%). Fifteen MLVA types were identified; 3-5-6-2 (41.7%), 4-5-7-2 (35.3%), and 3-6-6-2 (16.6%) were the major types, and four MLVA clusters were delineated. The distribution of MLVA types varied significantly over time and geographic location. The predominant MLVA type switched from 4-5-7-2 to 3-5-6-2 in 2015. MLVA type was associated with P1 subtypes and P1-2 variant types but not with macrolide resistance. To investigate the M. pneumoniae genotype shift and its impact on clinical presentations, additional surveillance programs targeting more diverse populations and prolonged sampling times are required.

scholarly journals Comparison of Molecular Characteristics of Mycoplasma pneumoniae Specimens Collected from the United States and China

2015 ◽  
Vol 53 (12) ◽  
pp. 3891-3893 ◽  
Author(s):  
Chao Yan ◽  
Hongmei Sun ◽  
Stella Lee ◽  
Rangaraj Selvarangan ◽  
Xuan Qin ◽  
...  
Keyword(s):  
United States ◽  
Mycoplasma Pneumoniae ◽  
The United States ◽  
Macrolide Resistance ◽  
Molecular Characteristics ◽  
Lower Prevalence ◽  
Content Type ◽  
Clinical Specimens ◽  
The U.S

Mycoplasma pneumoniae-positive clinical specimens obtained from the United States and China during the same period were studied for their molecular characteristics. We found much more diverse genotypes and a lower prevalence of macrolide resistance in the U.S. specimens. Data from the study also showed an association of the resistance with certain genotypes.

scholarly journals Investigations of Mycoplasma pneumoniae Infections in the United States: Trends in Molecular Typing and Macrolide Resistance from 2006 to 2013

2014 ◽  
Vol 53 (1) ◽  
pp. 124-130 ◽  
Author(s):  
Maureen H. Diaz ◽  
Alvaro J. Benitez ◽  
Jonas M. Winchell
Keyword(s):  
United States ◽  
Mycoplasma Pneumoniae ◽  
Diagnostic Testing ◽  
Variable Number Tandem Repeat ◽  
The United States ◽  
Macrolide Resistance ◽  
Severe Illness ◽  
Surveillance Program ◽  
Content Type ◽  
Time Period

Mycoplasma pneumoniaeis a leading cause of respiratory infections, including community-acquired pneumonia (CAP). Currently, pathogen-specific testing is not routinely performed in the primary care setting, and the United States lacks a systematic surveillance program forM. pneumoniae. Documentation of individual cases and clusters typically occurs only when severe illness and/or failure to improve with empirical antibiotic therapy is observed. Outbreaks, some lasting for extended periods and involving a large number of cases, occur regularly. However, many more likely go unrecognized due to the lack of diagnostic testing and structured reporting. We reviewed data from 17 investigations of cases, small clusters, and outbreaks ofM. pneumoniaeinfections that were supported by the Centers for Disease Control and Prevention (CDC) between 2006 and 2013. We examined 199M. pneumoniae-positive specimens collected during this time period in order to identify trends in antimicrobial resistance and circulating types. Overall, macrolide resistance was identified in approximately 10% ofM. pneumoniaeinfections occurring during this time period. Typing of strains revealed cocirculation of multiple multilocus variable-number tandem-repeat analysis (MLVA) and P1 types throughout this period, including diversity in types detected within individual outbreaks. Three MLVA types (4572, 3562, and 3662) accounted for 97% of the infections during the study period. A systematic surveillance program is necessary to understand the burden ofM. pneumoniaedisease in the United States, facilitate case and outbreak identification, and inform appropriate therapeutic and infection control strategies.

scholarly journals Macrolide-Resistant Mycoplasma pneumoniae in the United States as Determined from a National Surveillance Program

2019 ◽  
Vol 57 (11) ◽  
Author(s):  
K. B. Waites ◽  
A. Ratliff ◽  
D. M. Crabb ◽  
L. Xiao ◽  
X. Qin ◽  
...  
Keyword(s):  
United States ◽  
Clinical Significance ◽  
Mycoplasma Pneumoniae ◽  
The United States ◽  
Antimicrobial Treatment ◽  
23S Rrna ◽  
Surveillance Program ◽  
National Surveillance ◽  
Radiographic Findings ◽  
Content Type

ABSTRACT There are sparse data to indicate the extent that macrolide-resistant Mycoplasma pneumoniae (MRMp) occurs in the United States or its clinical significance. Between 2015 and 2018, hospitals in 8 states collected and stored respiratory specimens that tested positive for M. pneumoniae and sent them to the University of Alabama at Birmingham, where real-time PCR was performed for detection of 23S rRNA mutations known to confer macrolide resistance. MRMp was detected in 27 of 360 specimens (7.5%). MRMp prevalence was significantly higher in the South and East (18.3%) than in the West (2.1%). A2063G was the predominant 23S rRNA mutation detected. MICs for macrolide-susceptible M. pneumoniae (MSMp) were ≤0.008 μg/ml, whereas MICs for MRMp were 16 to 32 μg/ml. Patients with MRMp infection were more likely to have a history of immunodeficiency or malignancy. Otherwise, there were no other significant differences in the clinical features between patients infected with MRMp and those infected with MSMp, nor were there any differences in radiographic findings, hospitalization rates, viral coinfections, the mean duration of antimicrobial treatment, or clinical outcomes. There was no significant change in MRMp incidence over time or according to age, sex, race/ethnicity, or status as an inpatient or an outpatient. Patients with MRMp were more likely to have received a macrolide prior to presentation, and their treatment was more likely to have been changed to a fluoroquinolone after presentation. This is the first national surveillance program for M. pneumoniae in the United States. Additional surveillance is needed to assess the clinical significance of MRMp and to monitor changes in MRMp prevalence.

scholarly journals Emergence of Macrolide-Resistant Mycoplasma pneumoniae in Hong Kong Is Linked to Increasing Macrolide Resistance in Multilocus Variable-Number Tandem-Repeat Analysis Type 4-5-7-2

2015 ◽  
Vol 53 (11) ◽  
pp. 3560-3564 ◽  
Author(s):  
Pak-Leung Ho ◽  
Pierra Y. Law ◽  
Betsy W. K. Chan ◽  
Chun-Wai Wong ◽  
Kelvin K. W. To ◽  
...  
Keyword(s):  
Hong Kong ◽  
Mycoplasma Pneumoniae ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Routine Care ◽  
Variable Number ◽  
Macrolide Resistance ◽  
Content Type ◽  
Repeat Analysis ◽  
Mlva Type

Macrolide-resistantMycoplasma pneumoniae(MRMP) is rapidly emerging in Asia, but information on the temporal relationship between the increase in macrolide resistance and changes in strain types is scarce. Between 2011 and 2014,M. pneumoniaeinfection was diagnosed by PCR as part of routine care in a health care region in Hong Kong. Testing was initiated by clinicians, mainly in patients with suspectedM. pneumoniaepneumonia. Specimens positive forM. pneumoniaewere retrospectively investigated by macrolide resistance genotyping and a four-locus (Mpn13 to -16) multilocus variable-number tandem-repeat analysis (MLVA) scheme. The overall percentage ofM. pneumoniae-positive specimens was 17.9%, with annual rates ranging from 9.8% to 27.2%. The prevalence of MRMP had rapidly increased from 13.6% in 2011 to 30.7% in 2012, 36.6% in 2013, and 47.1% in 2014 (P= 0.038). Two major MLVA types, 4-5-7-2 and 3-5-6-2, accounted for 75% to 85% of the infections each year. MLVA types 4-5-7-2 and 3-5-6-2 predominated among macrolide-resistant and macrolide-sensitive groups, respectively. The increase in MRMP was mainly caused by increasing macrolide resistance in the prevalent MLVA type 4-5-7-2, changing from 25.0% in 2011 to 59.1% in 2012, to 89.7% in 2013, and to 100% in 2014 (P< 0.001). In conclusion, increasing MRMP in Hong Kong was linked to a single MLVA type, which was both prevalent and increasingly resistant to macrolides.

scholarly journals Evaluation of Commercial Molecular Diagnostic Methods for Detection and Determination of Macrolide Resistance in Mycoplasma pneumoniae

2020 ◽  
Vol 58 (6) ◽  
Author(s):  
Sixto M. Leal ◽  
Arthur H. Totten ◽  
Li Xiao ◽  
Donna M. Crabb ◽  
Amy Ratliff ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Large Scale ◽  
The United States ◽  
Macrolide Resistance ◽  
Diagnostic Methods ◽  
Respiratory Pathogen ◽  
Molecular Diagnostic ◽  
Content Type ◽  
Significant Difference ◽  
The Impact

ABSTRACT We evaluated six commercial molecular tests targeting Mycoplasma pneumoniae, namely, the BioFire FilmArray respiratory panel (RP), the Meridian Alethia Mycoplasma Direct, the GenMark ePlex respiratory pathogen panel (RPP), the Luminex NxTAG RPP, the ELITech ELITe InGenius Mycoplasma MGB research use only (RUO) PCR, and the SpeeDx Resistance Plus MP assays. Laboratory-developed PCR assays at the University of Alabama at Birmingham and the Centers for Disease Control and Prevention were used as reference standards. Among 428 specimens, 212 were designated confirmed positives for M. pneumoniae. The highest clinical sensitivities were found with the InGenius PCR (99.5%) and the FilmArray RP (98.1%). The Resistance Plus MP identified 93.3% of the confirmed-positive specimens, whereas 83.6, 64.6, and 55.7% were identified by the ePlex RPP, NxTAG RPP, and Mycoplasma Direct assays, respectively. There was no significant difference between the sensitivity of the reference methods and that of the FilmArray RP and InGenius assays, but the remaining four assays detected significantly fewer positive specimens (P < 0.05). Specificities of all assays were 99.5 to 100%. The Resistance Plus MP assay detected macrolide resistance in 27/33 specimens, resulting in a sensitivity of 81.8%. This study provides the first large-scale comparison of commercial molecular assays for detection of M. pneumoniae in the United States and identified clear differences among their performance. Additional studies are necessary to explore the impact of various test performances on patient outcome.

scholarly journals In Vitro Activities of Lefamulin and Other Antimicrobial Agents against Macrolide-Susceptible and Macrolide-Resistant Mycoplasma pneumoniae from the United States, Europe, and China

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Ken B. Waites ◽  
Donna M. Crabb ◽  
Lynn B. Duffy ◽  
Jorgen S. Jensen ◽  
Yang Liu ◽  
...  
Keyword(s):  
United States ◽  
Mycoplasma Pneumoniae ◽  
Antimicrobial Agents ◽  
The United States ◽  
Bactericidal Effect ◽  
Content Type ◽  
Highly Active ◽  
Resistant Strains

ABSTRACT Lefamulin, an investigational pleuromutilin, was tested against a collection of 18 macrolide-susceptible and 42 macrolide-resistant Mycoplasma pneumoniae strains, and the results were compared with those of azithromycin, erythromycin, tetracycline, doxycycline, and moxifloxacin testing. Lefamulin was highly active against all strains tested, with all MICs at ≤0.008 μg/ml. The lefamulin MIC90 (0.002 μg/ml) for macrolide-resistant strains was the lowest among all drugs tested. Minimum bactericidal concentrations were within 2 dilutions of the MIC values, indicating a bactericidal effect.

NCSS notable trade book lesson plan: the girl who buried her dreams in a can

2020 ◽  
Vol 15 (2) ◽  
pp. 121-126
Author(s):  
Takisha Durm
Keyword(s):  
United States ◽  
Research Design ◽  
Design Methodology ◽  
Lesson Plan ◽  
The United States ◽  
Elementary Grades ◽  
Effect Change ◽  
Content Type ◽  
Similarities And Differences ◽  
Future Date

PurposeThe Girl Who Buried Her Dreams in a Can, written by Dr Tererai, profiles a cultural, yet global experience of the power of believing in one's dream. Through this study of the similarities and differences of how children in the United States and abroad live and dream of a better life, this lesson seeks to enhance students' understandings of the power and authority they possess to effect change not only within their own lives but also in the lives of countless others in world. After reading the text, students will work to create vision boards illustrating their plans to effect change within their homes, schools, communities, states or countries. They will present their plans to their peers. To culminate the lesson, the students will bury their dreams in can and collectively decide on a future date to revisit the can to determine how far they have progressed in accomplishing their goals.Design/methodology/approachThis is an elementary grades 3–6 lesson plan. There was no research design/methodology/approach included.FindingsAs this is a lesson plan and no actual research was represented, there are no findings.Originality/valueThis is an original lesson plan completed by the first author Takisha Durm.

Bicycle Helmet Use Patterns Among Children

PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 166-173 ◽  
Author(s):  
Gregory B. Rodgers
Keyword(s):  
United States ◽  
Logistic Regression ◽  
Geographic Location ◽  
Personal Characteristics ◽  
The United States ◽  
National Sample ◽  
Medical Attention ◽  
Survey Method ◽  
Helmet Use ◽  
Use Patterns

Objective. To describe and evaluate the helmet use patterns of children younger than 15 years of age in the United States. Methods. A national telephone survey of bicycle riders was conducted by means of the Mitofsky-Waksberg method of random-digit dialing, a survey method intended to give all telephone numbers in the continental United States an equal probability of selection. Based on information collected in the survey, a logistic regression model was used to determine and quantify the factors associated with helmet use. Results. Information was collected on the bicycle and helmet use patterns of a national sample of 399 children younger than 15 years of age who rode bicycles during the year preceding the survey. This sample projects to the approximately 26.4 million children who are estimated to have ridden bicycles in 1991. About 26% of all child riders owned or had the use of bicycle helmets, and about 15% were reported to have used their helmets all or more than half of the time when riding. Information is provided on the reasons the children did or did not wear helmets. The logistic regression analysis shows that helmet use by children is systematically related to their personal characteristics (eg, age and whether they had previously had bicycle-related accidents requiring medical attention), riding patterns (eg, riding surface), and household demographic characteristics (eg, geographic location and whether household members had attended college). Conclusions. Helmet use rates among children remain low. Less than one fifth of the children who rode bicycles wore helmets all or more than half of the time in 1991. However, based on comparisons with earlier studies, the results of the analysis suggest that helmet use rates have been rising.

Washington will rethink its foreign chip strategy

2021 ◽  
Keyword(s):  
United States ◽  
Supply Chain ◽  
The United States ◽  
Export Controls ◽  
Political Pressure ◽  
Technical Standards ◽  
Content Type ◽  
The Us ◽  
Technology Competition ◽  
Business In China

Significance Follow-on action from Washington and responses from foreign actors will shape the US government’s adversarial policy towards China in semiconductors and other strategic technologies. Impacts The Biden administration will likely conclude that broad-based diversion of the semiconductor supply chain away from China is not feasible. The United States will rely on export controls and political pressure to prevent diffusion to China of cutting-edge chip technologies. The United States will focus on persuading foreign semiconductor leaders to help develop US capabilities, thereby staying ahead of China. Washington will focus on less direct approaches to strategic technology competition with China, notably technical standards-setting. Industry leaders in the semiconductor supply chain worldwide will continue expanding business in China in less politically sensitive areas.

Canada-US ties will see fewer irritants under Biden

2021 ◽  
Keyword(s):  
United States ◽  
Prime Minister ◽  
Oil Industry ◽  
The United States ◽  
Donald Trump ◽  
Federal Election ◽  
Content Type ◽  
Un Peacekeeping ◽  
Keystone Xl ◽  
Keystone Xl Pipeline

Significance Canada’s Liberal Prime Minister Justin Trudeau is preparing to welcome a more predictable and stable partner in Biden than outgoing Republican President Donald Trump. However, Biden is also expected quickly to cancel the Keystone XL pipeline, cutting another lifeline to Canada’s oil industry and creating some strain in Canada-US ties. Impacts Improved Canada-US ties will persist even if Trudeau loses the next federal election to Conservative Erin O’Toole. Canada will re-engage militarily with UN peacekeeping and NATO deployments. Trudeau will encourage Biden to end US prosecution of Meng Wanzhou, allowing Canada to release her; Biden may agree. Canada’s border with the United States will open in stages as COVID-19 recedes. Ottawa will push Biden to end ‘Buy American’ procurement policies, with little success.

Sign in / Sign up

Export Citation Format

Share Document