scholarly journals Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp.

2014 ◽  
Vol 53 (1) ◽  
pp. 302-308 ◽  
Author(s):  
Ana C. Q. Marques ◽  
Katia S. Paludo ◽  
Cibelle B. Dallagassa ◽  
Monica Surek ◽  
Fábio O. Pedrosa ◽  
...  
Keyword(s):  
Plant Growth Promoting Rhizobacteria ◽  
Eukaryotic Cells ◽  
Clinical Samples ◽  
Environmental Isolates ◽  
Biochemical Tests ◽  
Cytotoxic Effects ◽  
Content Type ◽  
Virulence Potential ◽  
Plant Growth Promoting ◽  
Growth Promoting

Herbaspirillumbacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. Here, biochemical tests, matrix-assisted laser deionization–time of flight (MALDI-TOF) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates ofHerbaspirillumspp. Discrete biochemical differences were observed between human and environmental strains. All strains adhered to HeLa cells at low densities, and cytotoxic effects were discrete, supporting the view thatHerbaspirillumbacteria are opportunists with low virulence potential.

scholarly journals Whole-Genome Sequences of Three Plant Growth-Promoting Rhizobacteria Isolated from Solanum tuberosum L. Rhizosphere in Tanzania

2020 ◽  
Vol 9 (20) ◽  
Author(s):  
Becky N. Aloo ◽  
Ernest R. Mbega ◽  
Billy A. Makumba ◽  
Ines Friedrich ◽  
Robert Hertel ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Plant Growth ◽  
Complete Genome ◽  
Solanum Tuberosum L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

We present here the complete genome sequences of plant growth-promoting Klebsiella sp. strain MPUS7, Serratia sp. strain NGAS9, and Citrobacter sp. strain LUTT5, isolated from rhizosphere soils and tubers of potato (Solanum tuberosum L.) plants growing in the northern and southern highlands of Tanzania.

scholarly journals Characterization of Selected Plant Growth-Promoting Rhizobacteria and Their Non-Host Growth Promotion Effects

2021 ◽  
Author(s):  
Di Fan ◽  
Donald L. Smith
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Content Type ◽  
Host Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

There are pressing needs to reduce the use of agrochemicals, and PGPR are receiving increasing interest in plant growth promotion and disease protection. This study follows up our previous report that the four newly isolated rhizobacteria promote the growth of Arabidopsis thaliana .

scholarly journals IAA production of indigenous isolate of plant growth promoting rhizobacteria in the presence of tryptophan

2020 ◽  
pp. 537-544 ◽  
Author(s):  
Maria José Cavalcante da Silva ◽  
Sebastião Ferreira Palmeira Junior ◽  
Kleber Fortes Junior ◽  
Velber Xavier Nascimento ◽  
Amanda Silva de Medeiros ◽  
...  
Keyword(s):  
Plant Growth ◽  
Indoleacetic Acid ◽  
Plant Growth Promoting Rhizobacteria ◽  
Automated System ◽  
Vegetable Production ◽  
Sequencing Data ◽  
Biochemical Tests ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting rhizobacteria (PGPRBs) is an interesting way to promote increased vegetable production. Here, we aimed to isolate, identify, and characterize PGPRBs by using biochemical tests, sequencing of 16S ribossomal DNA, in vitro and screening for indoleacetic acid production. The isolates were identified through VITEK® 2 Compact equipment, which is an automated system. We identified microorganisms such as Alcaligenes faecalis sp. faecalis, Pseudomonas putida, Proteus vulgaris, Providencia rettgeri, Serratia marcescens e Myroides sp. by performing vitek 2 biochemical tests. The analysis of sequencing data for 16S ribossomal DNA of the isolated bacteria showed presence of A. faecalis, Myroides sp., P. putida, P. vulgaris, Providencia sp. and Serratia sp. The in vitro screening of all isolated bacteria showed production of indoleacetic acid under presence of tryptophan, highlighting that higher concentrations were produced by Providencia sp. and Myroides sp. The rhizobacteria studied here have shown the potential to be used in the development of new products for plant growth-promoting.

scholarly journals Genome Sequences of Two Azospirillum sp. Strains, TSA2S and TSH100, Plant Growth-Promoting Rhizobacteria with N2O Mitigation Abilities

2019 ◽  
Vol 8 (32) ◽  
Author(s):  
Nan Gao ◽  
Weishou Shen ◽  
Tomoyasu Nishizawa ◽  
Kazuo Isobe ◽  
Yong Guo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Paddy Soil ◽  
Agricultural Soil ◽  
Plant Growth Promoting Rhizobacteria ◽  
Genome Sequences ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

Azospirillum sp. strains TSA2S and TSH100 are plant growth-promoting rhizobacteria with the capacity to mitigate N2O from agricultural soil. They were isolated from the rhizosphere of paddy soil in Tokyo, Japan. Here, we present the genome sequences of these two strains.

scholarly journals Draft Genome Sequences of Pseudomonas koreensis Strain UASWS1668, Bacillus megaterium Strain UASWS1667, and Paenibacillus sp. Strain UASWS1643, Considered Potential Plant Growth-Promoting Rhizobacteria

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Julien Crovadore ◽  
Bastien Cochard ◽  
Romain Chablais ◽  
Martine Haenzi ◽  
François Raffini ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Megaterium ◽  
Draft Genome ◽  
Plant Growth Promoting Rhizobacteria ◽  
Horse Chestnut ◽  
Genome Sequences ◽  
Content Type ◽  
Paenibacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Plant growth-promoting rhizobacteria (PGPR) include species in the genera Bacillus, Paenibacillus, and Pseudomonas. We report here the draft genome sequences of the strains Pseudomonas koreensis UASWS1668 and Bacillus megaterium UASWS1667, isolated from a horse chestnut tree, and Paenibacillus sp. strain UASWS1643, isolated from a tomato stem. Auxin production and phosphate solubilization were biochemically confirmed.

scholarly journals Plant growth-promoting rhizobacteria associated to candelilla rhizosphere (Euphorbia antisyphilitica) and its effects on Arabidopsis thaliana seedlings

2021 ◽  
Vol 49 (2) ◽  
pp. 12294
Author(s):  
Maria T. SALAZAR-RAMÍREZ ◽  
Jorge SÁENZ-MATA ◽  
Pablo PRECIADO-RANGEL ◽  
Manuel FORTIS-HERNÁNDEZ ◽  
Edgar O. RUEDA-PUENTE ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Plant Growth ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth Promoters ◽  
Biochemical Tests ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

In the communities of Sierra Mojada and Viesca, Coahuila, Mexico of Coahuila desert, two rhizosphere samplings of candelilla (Euphorbia antisyphilitica Zucc) were collected to isolate, characterize, and identifying plant growth-promoting rhizobacteria (PGPR); 165 rhizobacteria were tested in vitro with Arabidopsis thaliana seedlings to evaluate their potential as plant growth promoters, and obtaining 21 strains with best results in the variables of the number of secondary roots and fresh weight concerning the uninoculated control. Their salinity tolerance was evaluated at concentrations from 0.85 M, 1.7 M and 2.55 M of NaCl. Biochemical tests were accomplishing such as siderophores production, phosphates solubilization, production of Indole-3-acetic acid (IAA), and the activity of the ACC deaminase enzyme. The results obtained from 21 strains selected, high activities were obtained in organic substances like a siderophores since they developed a translucent orange halo around their growth; four rhizobacteria developed a clear halo around the bacterial growth with a thickness between 1.487 mm ± 0.667 mm and 5.267 mm ± 0.704 mm in phosphates solubilization; in the production of Indole-3-acetic acid (IAA), the bacterial strains showed the presence of this phytohormone, with values ​​from 4.444 μg mL-1 to 19.286 μg mL-1; and according to the activity of the ACC deaminase enzyme, values ​​from 0.424 to 1.306 µmol α-KB/h/mg Pr were showed. 16S rRNA sequencing was carried out and genus identified were Bacillus, Staphylococcus, Acinetobacter, Cronobacter and Siccibacter. The results obtained show the potential of the isolated rhizobacteria as growth promoters and the increase in the biomass of the Arabidopsis thaliana seedlings is evident. This is a first indication to proceed to carry out tests in different phenological stages in crops of agricultural importance.

scholarly journals Improved chromium tolerance of Medicago sativa by plant growth-promoting rhizobacteria (PGPR)

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Nabil Tirry ◽  
Aziza Kouchou ◽  
Bouchra El Omari ◽  
Mohamed Ferioun ◽  
Naïma El Ghachtouli
Keyword(s):  
Plant Growth ◽  
Medicago Sativa ◽  
Contaminated Soils ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Biochemical Tests ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background Soil pollution by heavy metals increases the bioavailability of metals like hexavalent chromium (Cr (VI)), subsequently limiting plant growth and reducing the efficiency of phytoremediation. Plant growth-promoting rhizobacteria (PGPR) have substantial potential to enhance plant growth as well as plant tolerance to metal stress. The aim of this research was to investigate Cr (VI) phytoremediation enhancement by PGPR. Results The results showed that the 27 rhizobacterial isolates studied were confirmed as Cr (VI)-resistant PGPR, by using classical biochemical tests (phosphate solubilization, nitrogen fixation, indole acetic acid, exopolysaccharides, hydrogen cyanide, siderophores, ammonia, cellulase, pectinase, and chitinase production) and showed variable levels of Cr (VI) resistance (300–600 mg/L). The best four selected Cr (VI)-resistant PGPR (NT15, NT19, NT20, and NT27) retained most of the PGP traits in the presence of 100–200 mg/L concentrations of Cr (VI). The inoculation of Medicago sativa with any of these four isolates improved the shoot and root dry weight. The NT27 isolate identified using 16S rDNA gene sequence analyses as a strain of Pseudomonas sp. was most effective in terms of plant growth promotion and stress level decrease. It increased shoot and root dry weights of M. sativa by 97.6 and 95.4%, respectively, in the presence of Cr (VI) when compared to non-inoculated control plants. It also greatly increased chlorophyll content and decreased the levels of stress markers, malondialdehyde, hydrogen peroxide, and proline. The results of the effect of Pseudomonas sp. on Cr content and bioaccumulation factor (BAF) of the shoots and roots of M. sativa plants showed the increase of plant biomass concomitantly with the increase of Cr root concentration in inoculated plants. This would lead to a higher potential of Cr (VI) phytostabilization. Conclusions This study demonstrates that the association M. sativa-Pseudomonas sp. may be an efficient biological system for the bioremediation of Cr (VI)-contaminated soils.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

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