scholarly journals Comparison of Real-Time PCR and Antigen Assays for Detection of Hepatitis E Virus in Blood Donors

2014 ◽  
Vol 52 (6) ◽  
pp. 2150-2156 ◽  
Author(s):  
T. Vollmer ◽  
C. Knabbe ◽  
J. Dreier
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E

scholarly journals Hepatitis-E-Virus-Infektion bei einem Patienten mit rheumatoider Arthritis unter Baricitinib-Therapie

2021 ◽  
Author(s):  
Larissa Valor-Méndez ◽  
Bernhard Manger ◽  
Georg Schett ◽  
Arnd Kleyer
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Patient Hatte

ZusammenfassungEs wurde ein Patient mit rheumatoider Arthritis (RA) vorgestellt, der unter der Therapie mit dem Januskinase-1/2-Inhibitor Baricitinib eine Infektion mit dem Hepatitis-E-Virus (HEV) entwickelte. Unser Patient hatte bei seiner vierteljährlichen Routineuntersuchung deutlich erhöhte Transaminasen bei einer unauffälligen körperlichen Untersuchung. Es fanden sich Antikörper der IgM- und IgG-Klasse gegen HEV und ein erhöhtes C‑reaktives Protein (CRP) sowie HEV-RNA mittels Real-Time-PCR, was auf eine frische HEV-Infektion hinwies. Baricitinib wurde sofort abgesetzt. Die ausführliche Anamnese ergab, dass der Patient Tage vor der Konsultation Rindertartar verzehrt hatte, ohne dass gastrointestinale Symptome oder Fieber aufgetreten waren. Im weiteren Verlauf erholte sich der Patient vollständig, und die Leberfunktionstests und das CRP normalisierten sich innerhalb von 3 Monaten. Baricitinib wurde daraufhin wieder eingenommen. Bisher wurden nur wenige Daten zu HEV-Infektionen bei RA-Patienten, die mit JAK-Inhibitoren behandelt wurden, publiziert.

scholarly journals Study of Hepatitis E Virus in Blood Donors

2019 ◽  
Vol 13 (1) ◽  
pp. 285-291
Author(s):  
Nashwa M. Al-Kasaby ◽  
Maysaa El Sayed Zaki
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Older Age ◽  
Rural Residence ◽  
Chain Reaction ◽  
Factors Associated ◽  
Polymerase Chain

Introduction: Hepatitis E (HEV) is a major health problem affecting around one third of the world population. The prevalence of antibodies to HEV among blood donors have been documented in several countries in Europe and Asia. Objectives: The aims of the study are to estimate the seroprevalence of hepatitis E antibodies among healthy blood donors and to explore the factors associated with positive HEV antibodies among healthy blood donors. Moreover, to detect HEV viremia by real time polymerase chain reaction among seropositive blood donors for HEV. Methods: The study included 200 apparent healthy blood donors from Dakahlia Governorate, Egypt. Blood samples were collected from the blood donors for serological determination for specific hepatitis E virus immunoglobulin G (anti-HEV IgG) and specific hepatitis E virus immunoglobulin M (anti- HEV IgM). Positive samples for anti-HEV IgM were further subjected for determination of HEV-RNA by real time Polymerase Chain Reaction (PCR). Anti-HEV-IgG was positive in 50 donor (25%) anti-HEV-IgM was positive in 10 donors (5%) and HEV-RNA was positive in 6 donors (3%). Results and Discussion: The comparison between blood donors positive for anti-HEV-IgG and negative blood donors negative reveals significant association between anti-HEV-IgG and donors with older age (42.0 ± 9.7,P = 0.001),rural residence (76%, P = 0.001), workers in agricultural works (92%, P = 0.035) and elevated AST (31.28±14.28, P = 0.04). Regarding viral markers, there was significant prevalance between positive anti-HCV-IgG and positive anti-HEV-IgG (P = 0.003). Univariate analysis for risk factors associated with positive anti-HEV IgG reveals significant prevalence with older age (P = 0.001), rural residence (P < 0.001), positive anti-HCV- IgG (P = 0.004) and increase in AST (P = 0.045). However, on Multivariate analysis HEV infection was independently prevalent with older age (P < 0.001) and rural residence (P = 0.002). Conclusion: The present study highlights that HEV seroprevalence in blood donors is common finding. Further finding is the statistically significant correlation between antibodies to HCV and serological markers for HEV and even HEV viremia. Longitudinal studies may be needed to explore the clinical significance and cost effectiveness of screening of the blood donors for hepatitis E virus by serological tests and/or detection of viremia by Molecular testing.

Universal detection of hepatitis E virus by two real-time PCR assays: TaqMan® and Primer-Probe Energy Transfer

2007 ◽  
Vol 146 (1-2) ◽  
pp. 226-235 ◽  
Author(s):  
Péter Gyarmati ◽  
Nahla Mohammed ◽  
Helene Norder ◽  
Jonas Blomberg ◽  
Sándor Belák ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Real Time Pcr ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Pcr Assays ◽  
Universal Detection

Minor groove binder modification of widely used TaqMan probe for hepatitis E virus reduces risk of false negative real-time PCR results

2012 ◽  
Vol 186 (1-2) ◽  
pp. 157-160 ◽  
Author(s):  
Jeremy A. Garson ◽  
Ruth B. Ferns ◽  
Paul R. Grant ◽  
Samreen Ijaz ◽  
Eleni Nastouli ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Hepatitis E Virus ◽  
False Negative ◽  
Hepatitis E ◽  
Minor Groove ◽  
Taqman Probe ◽  
Minor Groove Binder

scholarly journals Seroprevalence of hepatitis E virus in risk populations and blood donors in a referral hospital in the south of Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marisa Boff Costa ◽  
Michele Soares Gomes Gouvêa ◽  
Samira Chuffi ◽  
Gustavo Hirata Dellavia ◽  
Felipe Ornel ◽  
...  
Keyword(s):  
Low Income ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Total Sample ◽  
Hepatitis E ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Confirmatory Test ◽  
Cross Sectional ◽  
Populations At Risk

AbstractThe prevalence of anti-hepatitis E virus (HEV) antibodies has a high heterogeneity worldwide. South American data are still scarce. The aim of this study was to evaluate the prevalence of HEV in populations at risk in comparison to blood donors (BD). A cross-sectional study was carried out in adults of different risk populations including crack users (CK), residents in a low income area (LIA), cirrhotic (CIR) and liver transplant patients (LT) compared with BD. The WANTAI HEV ELISA test was used and real-time PCR (in-house for screening and ALTONA as confirmatory test) for HEV RNA screening. A total of 400 participants were included. Anti-HEV IgG was positive in 19.5% of the total sample, reaching the highest rate in the CIR group, 22.5%, followed by CK, LT, and LIA (20%, 18.7%, and 17.5%, respectively). The prevalence found in BD individuals was of 18.7% (p = NS). Anti-HEV IgM was positive in only 1.5% of the sample (6/400). No blood or stools samples were positive for HEV RNA. The seroprevalence reported is among the highest rates ever found in Brazil. Considering the intense diagnostic investigation, data show that HEV circulation is more common that might be expected in our country.

scholarly journals First Report of Hepatitis E Virus in Shellfish in Southeast Italy

2020 ◽  
Vol 11 (1) ◽  
pp. 43
Author(s):  
Gianfranco La Bella ◽  
Maria Grazia Basanisi ◽  
Gaia Nobili ◽  
Valentina Terio ◽  
Elisabetta Suffredini ◽  
...  
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Potential Role ◽  
Hepatitis E ◽  
Developed Countries ◽  
Rt Pcr ◽  
Viral Pathogens ◽  
Apulia Region ◽  
Causative Agents

Hepatitis E virus (HEV) represents one of the principal causative agents of hepatitis globally. Among the five HEV genotypes affecting humans, genotypes 3 and 4 are zoonotic and are the main source of hepatitis E in developed countries. HEV has been detected in several foods. The present work investigated the presence of this virus in shellfish sold at retail in the Apulia region of Italy. The presence of HEV RNA was assessed by real-time RT-PCR in 225 shellfish samples collected during 2018. Overall, two (0.89%) of these samples tested positive for HEV RNA. To our knowledge, this is the first notification of the detection of HEV in mussels sold at retail in the Apulia region. These data highlight the potential role of shellfish as a vehicle for the transmission of viral pathogens.

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