scholarly journals Differentiation among Members of the Mycobacterium tuberculosis Complex by Molecular and Biochemical Features: Evidence for Two Pyrazinamide-Susceptible Subtypes of M. bovis

2000 ◽  
Vol 38 (1) ◽  
pp. 152-157
Author(s):  
Stefan Niemann ◽  
Elvira Richter ◽  
Sabine Rüsch-Gerdes
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Clinical Importance ◽  
Acid Hydrazide ◽  
Biochemical Properties ◽  
Molecular Characteristics ◽  
Tuberculosis Complex ◽  
Carboxylic Acid Hydrazide ◽  
Spoligotype Pattern ◽  
Group 3

ABSTRACT The variations in biochemical as well as molecular characteristics among several members of the Mycobacterium tuberculosis complex that are not M. tuberculosis have been assessed to facilitate an unambiguous species identification. Altogether, 96 M. tuberculosis complex strains including 52 M. bovis isolates and 44 M. africanum isolates were analyzed by spoligotyping. The strains could be clustered into five spoligotype groups. All M. bovis isolates showed the typical absence of the spacers 39 to 43 and typical biochemical properties. However, within these strains we found a group of strains that had a spoligotype pattern which is clearly defined by the additional absence of spacers 3 to 16 and that were uncommonly susceptible to pyrazinamide (PZA). This spoligotype pattern has previously been described as being typical for a caprine genotype because of its predominant isolation from sheep and goats. Due to the clinical importance of PZA resistance, we propose two M. bovis subtypes: M. bovis subtype bovis , which is resistant to PZA, and M. bovis subtype caprae , which is susceptible to PZA. Two additional strains that clustered in group 3 showed biochemical and genetic properties typical for M. bovis and were also sensitive to PZA; thus, they may represent a third PZA-susceptible M. bovis subtype. The M. africanum isolates could be clustered into two spoligotype groups which can be differentiated from M. bovis by hybridization to spacers 39 to 43. These groups correspond to the previously described M. africanum subtypes I and II and can be clearly distinguished from each other by spoligotyping and resistance to thiophen-2-carboxylic acid hydrazide. Our results demonstrate that spoligotyping is a useful tool for differentiation of M. bovis and M. africanum . Moreover, we describe two PZA-susceptible M. bovis subtypes and describe a method that facilitates an unambiguous differentiation of the two M. africanum subtypes.

SPOLIGOBD VER4 – A GENOTYPE DATABASE OF SPOLIGOTYPING FOR MYCOBACTERIUM TUBERUCULOSIS COMPLEX

2014 ◽  
pp. 90-97
Author(s):  
Hoang Bach Nguyen ◽  
Thi Hai Duong Huynh ◽  
Viet Quynh Tram Ngo
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Reference Database ◽  
Complex Object ◽  
Tuberculosis Complex ◽  
Analysis Process ◽  
Spoligotype Pattern ◽  
Result Analysis ◽  
Mycobacteria Tuberculosis

Study objectives: To build a database recording all types of spoligotyping genes to serve the purpose of analyzing the result of spoligotyping technique in the study of the genetic diversity of Mycobacteria tuberculosis complex. Object and Methods: To use SpolDB4 as the reference database from Guadeloupe Paster Institute to employ PHP language for programming and record the spoligotyping data in MySQL. The module is run on the web platform. To build statistical functions according to different data entries and different searching tools according to various data input. Results: To complete the building of “SpoligoDB ver4” database to meet the demand of fast, visual and exact searching requirements and to fulfill the result testing process on spoligotyping technique among researches on classifications and drug-resistance and of Mycobacterium tuberculosis complex at Carlo Urbani Center, Hue University of Medicine and Pharmacy and other agencies at the following address http://khdn-yhue.vn/modules.php?name=Spoligodb; http://carlo-urbani-center.org/en/modules.php?name=Spoligodb. Conclusion: To complete the building of SpologoDB ver4 database from enucleated SpolDB4 database provided by the Guadeloupe Pasteur Institute and meet its requirements to serve the result analysis process on spoligotyping technique for those units that wish to research on the genetic diversity of Mycobacterium tuberculosis complex. Keywords: spoligotyping, spoligotype pattern, SpoligoDB ver4, AIE-VNM.

scholarly journals Genome structure in the vole bacillus, Mycobacterium microti, a member of the Mycobacterium tuberculosis complex with a low virulence for humans

Microbiology ◽  
2004 ◽  
Vol 150 (5) ◽  
pp. 1519-1527 ◽  
Author(s):  
Cristiane C. Frota ◽  
Debbie M. Hunt ◽  
Roger S. Buxton ◽  
Lisa Rickman ◽  
Jason Hinds ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Genome Structure ◽  
Biochemical Properties ◽  
Extensive Study ◽  
Tuberculosis Complex ◽  
Almost All ◽  
Mycobacterium Microti ◽  
Different Levels ◽  
Immunocompetent Patients

Mycobacterium microti, a member of the Mycobacterium tuberculosis complex, is phylogenetically closely related to M. tuberculosis, differing in a few biochemical properties. However, these species have different levels of virulence in different hosts; most notably M. microti shows lower virulence for humans than M. tuberculosis. This report presents genomic comparisons using DNA microarray analysis for an extensive study of the diversity of M. microti strains. Compared to M. tuberculosis H37Rv, 13 deletions were identified in 12 strains of M. microti, including the regions RD1 to RD10, which are also missing in Mycobacterium bovis BCG. In addition, four new deleted regions, named MiD1, RD1β, MiD2 and MiD3, were identified. DNA sequencing was used to define the extent of most of the deletions in one strain. Although RD1 of M. bovis BCG and M. microti is thought to be crucial for attenuation, in this study, three of the four M. microti strains that were isolated from immunocompetent patients had the RD1 deletion. In fact, only the RD3 deletion was present in all of the strains examined, although deletions RD7, RD8 and MiD1 were found in almost all the M. microti strains. These deletions might therefore have some relation to the different host range of M. microti. It was also noticeable that of the 12 strains studied, only three were identical; these strains were all isolated from immunocompetent humans, suggesting that they could have arisen from a single source. Thus, this study shows that it is difficult to ascribe virulence to any particular pattern of deletion in M. microti.

scholarly journals Spatial and Temporal Distribution of Mycobacterium tuberculosis Complex Infection in Eurasian Badger (Meles meles) and Cattle in Asturias, Spain

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1294
Author(s):  
Cristina Blanco Blanco Vázquez ◽  
Thiago Doria Barral ◽  
Beatriz Romero ◽  
Manuel Queipo ◽  
Isabel Merediz ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Road Traffic ◽  
Mycobacterium Tuberculosis Complex ◽  
Temporal Distribution ◽  
Meles Meles ◽  
Interspecies Transmission ◽  
Tuberculosis Complex ◽  
Serum Samples ◽  
Study Objective ◽  
Infection Pressure

The present work investigated the prevalence, spatial distribution, and temporal distribution of tuberculosis (TB) in free-ranging Eurasian badgers (Meles meles) and cattle in Asturias (Atlantic Spain) during a 13-year follow-up. The study objective was to assess the role of badgers as a TB reservoir for cattle and other sympatric wild species in the region. Between 2008 and 2020, 673 badgers (98 trapped and 575 killed in road traffic accidents) in Asturias were necropsied, and their tissue samples were cultured for the Mycobacterium tuberculosis complex (MTC) isolation. Serum samples were tested in an in-house indirect P22 ELISA to detect antibodies against the MTC. In parallel, data on MTC isolation and single intradermal tuberculin test results were extracted for cattle that were tested and culled as part of the Spanish National Program for the Eradication of Bovine TB. A total of 27/639 badgers (4.23%) were positive for MTC based on bacterial isolation, while 160/673 badgers (23.77%) were found to be positive with the P22 ELISA. The rate of seropositivity was higher among adult badgers than subadults. Badger TB status was spatially and temporally associated with cattle TB status. Our results cannot determine the direction of possible interspecies transmission, but they are consistent with the idea that the two hosts may exert infection pressure on each other. This study highlights the importance of the wildlife monitoring of infection and disease during epidemiological interventions in order to optimize outcomes.

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