scholarly journals Shedding Light on the Role of the Skin in Vaccine-Induced Protection against the Malaria Sporozoite

mBio ◽  
2018 ◽  
Vol 9 (6) ◽  
Author(s):  
Johanna Patricia Daily
Keyword(s):  
Plasmodium Falciparum ◽  
Blood Vessels ◽  
Blood Stream ◽  
Mosquito Vectors ◽  
Protective Mechanisms ◽  
Partial Protection ◽  
Content Type ◽  
Shed Light ◽  
In Infants And Children

ABSTRACT The most advanced vaccine against Plasmodium falciparum malaria, RTS,S/AS01, provides partial protection in infants and children living in areas of malaria endemicity. Further understanding its mechanisms of protection may allow the development of improved second-generation vaccines. The RTS,S/AS01 vaccine targets the sporozoites injected by mosquito vectors into the dermis which then travel into the blood stream to establish infection in the liver. Flores-Garcia et al. (Y. Flores-Garcia, G. Nasir, C. S. Hopp, C. Munoz, et al., mBio 9:e02194-18, 2018, https://doi.org/10.1128/mBio.02194-18) shed light on early protective responses occurring in the dermis in immunized animals. They demonstrated that immunization impairs sporozoite motility and entry into blood vessels. Furthermore, they established that challenge experiments performed using a dermal route conferred greater protection than intravenous challenge in immunized mice. Thus, the dermal challenge approach captures the additional protective mechanisms occurring in the dermis that reflect the natural physiology of infection. Those studies highlighted the fascinating biology of skin-stage sporozoites and provided additional insights into vaccine-induced protection.

The role of Staphylococcus lugdunensis as a pathogen in children: a multicentre retrospective study

2021 ◽  
Vol 70 (5) ◽  
Author(s):  
Noa Hurvitz ◽  
Lea Ohana Sarna Cahan ◽  
Itai Gross ◽  
Daniel Grupel ◽  
Orli Megged ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Blood Culture ◽  
Otitis Media ◽  
Paediatric Population ◽  
Blood Stream ◽  
Staphylococcus Lugdunensis ◽  
Content Type ◽  
Link Type ◽  
Putative Role

Introduction: Staphylococcus lugdunensis (SL), a tube coagulase negative Staphylococcus , is known to be pathogenic in adults, causing mainly skin infections. Gap Statement: Previous studies assessing SL's role in paediatric populations are sparse and are mainly limited to case reports. Aim: Present the clinical characteristics consistent with SL infections and its putative role as a pathogen in the paediatric population. Methodology: A retrospective multicentre study was conducted in four paediatric medical centres in Israel. Patients with isolates of SL presenting between 2009–2019 were included. Results: SL was isolated from 40 patients. Average (±SD) age at presentation was 5.9 (±6.2) years, with 22 (55 %) being female. Skin, soft tissue and musculoskeletal infections were the most common (n=20, 50%) followed by ear infections (n=13, 32.5%). Five cases of urine isolates and two isolates from blood culture samples were also reported. Skin abscess was the most common infection among skin and soft tissue isolates, reported in 17 children (85%) with SL being the only pathogen in 15 (75%). Otitis media was the most common ear infection accounting for 12 (92%) of all cases with SL as the only isolate reported in 6 (46%). Five cases of SL isolates from urine specimens were reported, all of which with poor growth of bacteria and normal urinalysis. Two cases of SL growth in blood culture were found in children presenting with signs and symptoms consistent with invasive blood stream infection. Conclusions: In the paediatric population, studied infections caused by SL are increasingly observed. The results of this study highlight its role as a pathogen in soft tissue infections and its putative role in otitis media and invasive blood stream infections. However, the role of SL as an uropathogen was not established.

scholarly journals Combined Transcriptome and Proteome Profiling for Role of pfEMP1 in Antimalarial Mechanism of Action of Dihydroartemisinin

2021 ◽  
Author(s):  
Lina Chen ◽  
Zhongyuan Zheng ◽  
Hui Liu ◽  
Xi Wang ◽  
Shuiqing Qu ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Mechanism Of Action ◽  
Blood Cells ◽  
Artemisinin Derivative ◽  
Combination Therapies ◽  
Proteomic Profiling ◽  
First Line ◽  
Content Type ◽  
Artemisinin Combination Therapies

Malaria parasites induce morphological and biochemical changes in the membranes of parasite-infected red blood cells (iRBCs) for propagation, with artemisinin combination therapies as the first-line treatments. To understand whether artemisinin targets or interacts with iRBC membrane proteins, this study investigated the molecular changes caused by dihydroartemisinin (DHA), an artemisinin derivative, in Plasmodium falciparum 3D7 using a combined transcriptomic and membrane proteomic profiling approach.

scholarly journals THE FATE OF EXOGENOUS PEROXIDASE IN THE THYMUS OF NEWBORN AND YOUNG ADULT MICE

1972 ◽  
Vol 20 (6) ◽  
pp. 445-462 ◽  
Author(s):  
SUSAN WINTER GERVIN ◽  
ERIC HOLTZMAN
Keyword(s):  
Young Adult ◽  
Horseradish Peroxidase ◽  
Blood Vessels ◽  
Blood Stream ◽  
Active Uptake ◽  
Adult Mice ◽  
Pinocytotic Vesicles ◽  
Parenchymal Cells ◽  
Shed Light

Horseradish peroxidase injected intraperitoneally into newborn and young adult mice is subsequently found in the thymus within the lumina of blood vessels and in perivascular areas. In the newborns, much peroxidase is also detectable throughout the thymic parenchyma; it is present in extracellular spaces and in pinocytotic vesicles and lysosomes in thymocytes and other parenchymal cells. In young adult mice, very little peroxidase escapes from the vicinity of blood vessels; cells resembling macrophages contain most of the tracer present outside of blood vessels and only those thymocytes located very near small venules or capillaries are exposed to peroxidase. The results suggest that the "blood-thymic" barrier is not simply a static set of structures that prevents penetration of potential antigens from the blood stream into the parenchyma; in young adult animals, active uptake of foreign molecules by macrophages and other cells is an important component of the barrier. In addition, the findings may shed light on aspects of the development of the immunologic system, since it appears, for example, that potential antigens can interact directly with far more cells in the newborn thymus than is true in the adult.

scholarly journals The paradox of being on the glass cliff: why do women accept risky leadership positions?

2018 ◽  
Vol 23 (4) ◽  
pp. 397-426 ◽  
Author(s):  
Maral Darouei ◽  
Helen Pluut
Keyword(s):  
Decision Makers ◽  
Risk Averse ◽  
Job Seekers ◽  
Leadership Positions ◽  
Apparent Paradox ◽  
Content Type ◽  
Working Adults ◽  
Glass Cliff ◽  
Shed Light

PurposeRecent evidence from glass cliff research suggests that women are more willing than men to accept risky leadership positions. The purpose of this paper (based on three studies) is to reveal and resolve the apparent paradox that women are more risk averse than men yet end up in risky leadership positions.Design/methodology/approachIn Study I, risk attitudes of 125 participants were surveyed to understand gender differences in risk taking. In two experimental vignette studies, 119 university students (Study II) and 109 working adults (Study III) were offered a leadership position in either a risky or successful company and asked to rate their willingness to accept the job.FindingsTogether, the results showed that although women are generally more risk averse than men, women who scored low on career self-efficacy were more likely to perceive a risky job as a promotional opportunity and were therefore more willing to accept such a job. These findings shed light on the role of women’s career decision making in the glass cliff phenomenon.Originality/valueGlass cliff research has focused almost exclusively on organizational decision makers. The authors aim to better understand the glass cliff phenomenon by incorporating the perspective of job seekers.

scholarly journals From Nursery to Nursing Home: Emerging Concepts in Clostridioides difficile Pathogenesis

2020 ◽  
Vol 88 (7) ◽  
Author(s):  
Alexander B. Smith ◽  
Joshua Soto Ocana ◽  
Joseph P. Zackular
Keyword(s):  
Pharmaceutical Drugs ◽  
Human Gastrointestinal Tract ◽  
Content Type ◽  
The Past ◽  
Clostridioides Difficile ◽  
Wide Range ◽  
Pediatric Populations ◽  
The Impact ◽  
Shed Light

ABSTRACT Clostridioides difficile is a Gram-positive, spore-forming, anaerobic bacterium that infects the human gastrointestinal tract, causing a wide range of disorders that vary in severity from mild diarrhea to toxic megacolon and/or death. Over the past decade, incidence, severity, and costs associated with C. difficile infection (CDI) have increased dramatically in both the pediatric and adult populations. The factors driving this rapidly evolving epidemiology remain largely unknown but are likely due in part to previously unappreciated host, microbiota, and environmental factors. In this review, we will cover the risks and challenges of CDI in adult and pediatric populations and examine asymptomatic colonization in infants. We will also discuss the emerging role of diet, pharmaceutical drugs, and pathogen-microbiota interactions in C. difficile pathogenesis, as well as the impact of host-microbiota interactions in the manifestation of C. difficile-associated disease. Finally, we highlight new areas of research and novel strategies that may shed light on this complex infection and provide insights into the future of microbiota-based therapeutics for CDI.

scholarly journals What is an author now? Discourse analysis applied to the idea of an author

2015 ◽  
Vol 71 (5) ◽  
pp. 1094-1114 ◽  
Author(s):  
Daniel Martínez-Ávila ◽  
Richard Smiraglia ◽  
Hur-Li Lee ◽  
Melodie Fox
Keyword(s):  
Discourse Analysis ◽  
Lived Experience ◽  
Design Methodology ◽  
Public Discourse ◽  
Phenomenological Analysis ◽  
Content Type ◽  
The Public ◽  
Intellectual Content ◽  
Shed Light

Purpose – The purpose of this paper is to discuss and shed light on the following questions: What is an author? Is it a person who writes? Or, is it, in information, an iconic taxonomic designation (some might say a “classification”) for a group of writings that are recognized by the public in some particular way? What does it mean when a search engine, or catalog, asks a user to enter the name of an author? And how does that accord with the manner in which the data have been entered in association with the names of the entities identified with the concept of authorship? Design/methodology/approach – The authors use several cases as bases of phenomenological discourse analysis, combining as best the authors can components of eidetic bracketing (a Husserlian technique for isolating noetic reduction) with Foucauldian discourse analysis. The two approaches are not sympathetic or together cogent, so the authors present them instead as alternative explanations alongside empirical evidence. In this way the authors are able to isolate components of iconic “authorship” and then subsequently engage them in discourse. Findings – An “author” is an iconic name associated with a class of works. An “author” is a role in public discourse between a set of works and the culture that consumes them. An “author” is a role in cultural sublimation, or a power broker in deabstemiation. An “author” is last, if ever, a person responsible for the intellectual content of a published work. The library catalog’s attribution of “author” is at odds with the Foucauldian discursive comprehension of the role of an “author.” Originality/value – One of the main assets of this paper is the combination of Foucauldian discourse analysis with phenomenological analysis for the study of the “author.” The authors turned to Foucauldian discourse analysis to discover the loci of power in the interactions of the public with the named authorial entities. The authors also looked to phenomenological analysis to consider the lived experience of users who encounter the same named authorial entities. The study of the “author” in this combined way facilitated the revelation of new aspects of the role of authorship in search engines and library catalogs.

A role of the central catecholamine neuron in cerebral circulation

1986 ◽  
Vol 65 (3) ◽  
pp. 370-375 ◽  
Author(s):  
Hideyoshi Yokote ◽  
Toru Itakura ◽  
Kunio Nakai ◽  
Ichiro Kamei ◽  
Harumichi Imai ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Blood Vessels ◽  
Cerebral Circulation ◽  
Intracerebral Injection ◽  
Neuron System ◽  
Content Type ◽  
6 Hydroxydopamine ◽  
Catecholamine Neuron

✓ The effect of the central catecholaminergic neurons on the cerebral microcirculation was investigated by means of a unilateral intracerebral injection of 6-hydroxydopamine (6-OHDA) which produced the degeneration of catecholamine (CA) nerve terminals. Subsequent observation with CA histofluorescence revealed an absence of CA fibers in the vicinity of the 6-OHDA injection site. A significant increase in regional cerebral blood flow (rCBF), measured by the hydrogen clearance method, was demonstrated in the CA-depleted cortex under normocapnia as compared with rCBF in the control cortex (CA-depleted cortex 47.0 ± 2.8 ml/100 gm/min; control cortex 38.5 ± 3.5 ml/100 gm/min; p < 0.005). The increased rCBF in the cortex treated with 6-OHDA was suppressed by the iontophoretic replacement of noradrenaline (NA) to the CA-depleted cortex. An iontophoretic replacement of 10−5 M dopamine (DA) mildly suppressed the increased rCBF in the 6-OHDA-treated cortex. The CO2 reactivity in the CA-depleted cortex was significantly lower than that of the control cortex (CA-depleted cortex 2.13% ± 0.67%/mm Hg; control cortex 3.53% ± 0.70%/mm Hg). No change was noticeable in the cerebral glucose metabolism in the CA-depleted cortex in an investigation based on tritiated (3H)-deoxyglucose uptake. It is suggested that the 6-OHDA-induced change in cerebral blood flow (CBF) is not secondary to alterations in cerebral metabolic rate, and that the central NA neuron system innervating intraparenchymal blood vessels regulates CBF through a direct vasoconstrictive effect on the cerebral blood vessels. The central DA neuron system may modulate the cerebral circulation as a mild vasoconstrictor.

Unemployment outflows: the relevance of gender and marital status in Italy and Spain

2014 ◽  
Vol 35 (5) ◽  
pp. 594-612 ◽  
Author(s):  
Chiara Mussida ◽  
Enrico Fabrizi
Keyword(s):  
Marital Status ◽  
Design Methodology ◽  
Geographical Area ◽  
International Comparisons ◽  
Panel Survey ◽  
Labour Mobility ◽  
Content Type ◽  
Status Differences ◽  
Shed Light

Purpose – The purpose of this paper is to shed light on transitions from the state of unemployment to that of employment and of inactivity in Italy and Spain. Design/methodology/approach – First, the paper investigates the determinants of unemployment outflows in these two Mediterranean labour markets. Then, the paper examines discrepancies and similarities between specific outflow determinants, especially the interactions between gender and marital status, by comparing results obtained across countries. Findings – The findings of the paper suggest that gender and marital status influence the probability of unemployment outflows in both countries, although not in the same way, especially with reference to marital status. Discrepancies also emerge in relation to the role of geographical area of residence. Originality/value – International comparisons of unemployment outflows are rather new in the literature, and as far as we know none have been performed using European Union Statistics on Income and Living Conditions data. Further, although studies quite often examine the issue of gender-related labour mobility using the European Community Household Panel survey that took place in the 1990s (Arulampalam et al., 2007; Garcia Pérez and Rebollo Sanz, 2005; Theodossiou and Zangelidis, 2009), one of the main contributions of this paper is that it provides a systematic examination of the issue, considering the influence of gender and marital status differences on patterns of unemployment outflows to employment and inactivity.

Credibility of a peer endorser and advertising effectiveness

2016 ◽  
Vol 33 (3) ◽  
pp. 182-192 ◽  
Author(s):  
Juha Munnukka ◽  
Outi Uusitalo ◽  
Hanna Toivonen
Keyword(s):  
Design Methodology ◽  
Advertising Effectiveness ◽  
Product Involvement ◽  
Online Questionnaire ◽  
Content Type ◽  
Brand Involvement ◽  
Endorser Credibility ◽  
Shed Light ◽  
Brand Product

Purpose Advertisers use various tactics to influence consumer purchases and create positive associations with their brands. The purpose of this study is to explore the formation of peer-endorser credibility and its influence on attitude formation. The role of product involvement in the formation of attitudes and endorser credibility is also examined. Design/methodology/approach A quantitative study was conducted among university students. Data were collected using an online questionnaire concerning three advertisements for which identical questionnaires were constructed; 364 responses were generated. Findings The authors show that the credibility of a peer endorser is constructed from trustworthiness, expertise, similarity and attractiveness dimensions that positively affect consumers’ attitude toward an advertisement and a brand. Product involvement affects advertising effectiveness indirectly through the endorser-credibility construct. Finally, the authors show that a consumer’s experience with an advertised product affects the perception of endorser credibility and the effectiveness of the advertisement. Originality/value The findings reveal new insights into the little studied area of peer-endorser effectiveness. The authors shed light on the construction of peer endorser credibility and the relative importance of specific credibility dimensions on the effectiveness of an advertisement. This study also provides information on the direct and indirect effects of consumers’ brand involvement on attitudes toward advertisements.

scholarly journals Emotional strategizing in service innovation

2016 ◽  
Vol 54 (2) ◽  
pp. 270-287 ◽  
Author(s):  
Catharina von Koskull ◽  
Tore Strandvik ◽  
Bård Tronvoll
Keyword(s):  
Design Methodology ◽  
Service Innovation ◽  
Innovation Process ◽  
Ethnographic Study ◽  
Strategy Research ◽  
Content Type ◽  
Innovation Processes ◽  
Practical Implications ◽  
Shed Light

Purpose – The purpose of this paper is to shed light on an aspect of service innovation processes that has remained fairly hidden so far, namely, the role of emotions. Design/methodology/approach – The authors use the strategizing approach from strategy research, which focusses on detailed processes, practices, and discourse, to understand the influence of emotions on service innovation processes. The empirical data stem from a longitudinal ethnographic study of a service innovation process. Findings – In the investigated case, the dominant emotion of anxiety is revealed. The authors focus on this emotion in order to explore how it affects the innovation process itself and the outcome. The authors identify five emotion-driven practices that form elements of what the authors label emotional strategizing. Practical implications – Emotion seems to give energy and direction to the service innovation process. This is both positive and challenging for top-level managers. Originality/value – The authors reveal a hidden aspect of service innovation processes – the effect of emotions. Furthermore, the authors show that emotions are important because they give energy and direction to the innovation work, and emerge in practices. Emotional strategizing, as a new term, gives visibility to this important issue.

Sign in / Sign up

Export Citation Format

Share Document