scholarly journals Glycan-Dependent Corneocyte Adherence of Staphylococcus epidermidis Mediated by the Lectin Subdomain of Aap

mBio ◽  
2021 ◽  
Author(s):  
Paroma Roy ◽  
Alexander R. Horswill ◽  
Paul D. Fey
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Staphylococcus Epidermidis ◽  
Normal Skin ◽  
Toxic Substances ◽  
Soft Tissue Infections ◽  
Healthy Individuals ◽  
Content Type ◽  
Skin Flora

Staphylococcus aureus is the most significant cause of skin and soft tissue infections yet it rarely colonizes the skin of healthy individuals. This is believed to be due, in part, to inhibition of colonization via toxic substances produced by normal skin flora, including by S. epidermidis .

scholarly journals Role of Antibodies in Protection Elicited by Active Vaccination with Genetically Inactivated Alpha Hemolysin in a Mouse Model of Staphylococcus aureus Skin and Soft Tissue Infections

2014 ◽  
Vol 21 (5) ◽  
pp. 622-627 ◽  
Author(s):  
Christopher P. Mocca ◽  
Rebecca A. Brady ◽  
Drusilla L. Burns
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Murine Model ◽  
Bacterial Colonization ◽  
Passive Immunization ◽  
The United States ◽  
Soft Tissue Infections ◽  
Content Type ◽  
Alpha Hemolysin ◽  
Active Vaccination

ABSTRACTDue to the emergence of highly virulent community-associated methicillin-resistantStaphylococcus aureus(CA-MRSA) infections,S. aureushas become a major threat to public health. A majority of CA-MRSA skin and soft tissue infections in the United States are caused byS. aureusUSA300 strains that are known to produce high levels of alpha hemolysin (Hla). Therefore, vaccines that contain inactivated forms of this toxin are currently being developed. In this study, we sought to determine the immune mechanisms of protection for this antigen using a vaccine composed of a genetically inactivated form of Hla (HlaH35L). Using a murine model of skin and soft tissue infections (SSTI), we found that BALB/c mice were protected by vaccination with HlaH35L; however, Jh mice, which are deficient in mature B lymphocytes and lack IgM and IgG in their serum, were not protected. Passive immunization with anti-HlaH35L antibodies conferred protection against bacterial colonization. Moreover, we found a positive correlation between the total antibody concentration induced by active vaccination and reduced bacterial levels. Animals that developed detectable neutralizing antibody titers after active vaccination were significantly protected from infection. These data demonstrate that antibodies to Hla represent the major mechanism of protection afforded by active vaccination with inactivated Hla in this murine model of SSTI, and in this disease model, antibody levels correlate with protection. These results provide important information for the future development and evaluation ofS. aureusvaccines.

scholarly journals High Staphylococcus aureus Colonization Prevalence among Patients with Skin and Soft Tissue Infections and Controls in an Urban Emergency Department

2014 ◽  
Vol 53 (3) ◽  
pp. 810-815 ◽  
Author(s):  
Neha Kumar ◽  
Michael Z. David ◽  
Susan Boyle-Vavra ◽  
Julia Sieth ◽  
Robert S. Daum
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
The United States ◽  
Antimicrobial Treatment ◽  
Soft Tissue Infections ◽  
Content Type ◽  
Treatment Regimens ◽  
Commensal Species ◽  
High Prevalence

Staphylococcus aureusis a commensal species that can also be a formidable pathogen. In the United States, an epidemic of community-acquired methicillin-resistantStaphylococcus aureus(MRSA) infections has been occurring for the last 15 years. In the context of a study in which we identified patients with skin and soft tissue infections (SSTIs) and randomized them to receive one of two antimicrobial treatment regimens, we assessedS. aureuscolonization in the nares, throat, and perianal skin on the day of enrollment and 40 days after therapy. We compared the prevalence of colonization between the SSTI patients and an uninfected control population. A total of 144 subjects and 130 controls, predominantly African American, participated in this study, and 116 returned for a 40-day follow-up visit. Of the SSTI patients, 76% were colonized withS. aureusat enrollment, as were 65% of the controls. Patients were more likely than the controls to be colonized with USA300 MRSA (62/144 [43.1%] versus 11/130 [8.5%], respectively;P< 0.001). The nares were not the most common site of colonization. The colonization prevalence diminished somewhat after antibiotic treatment but remained high. The isolates that colonized the controls were more likely than those in the patients to be methicillin-susceptibleS. aureus(MSSA) (74/84 [88.1%] versus 56/106 [52.8%], respectively;P< 0.001). In conclusion, the prevalence ofS. aureuscolonization among SSTI patients was high and often involved USA300 MRSA. The prevalence diminished somewhat with antimicrobial therapy but remained high at the 40-day follow-up visit. Control subjects were also colonized at a high prevalence but most often with a genetic background not associated with a clinical infection in this study.S. aureusis a commensal species and a pathogen. Plans for decolonization or eradication should take this distinction into account.

scholarly journals Whole-Genome Sequences of Staphylococcus aureus Isolates from Positive Blood Cultures

2021 ◽  
Vol 10 (43) ◽  
Author(s):  
Itidal Reslane ◽  
Margaret Sladek ◽  
Paul D. Fey ◽  
Baha Abdalhamid
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Draft Genome ◽  
Bloodstream Infections ◽  
Blood Cultures ◽  
Whole Genome ◽  
Soft Tissue Infections ◽  
Genome Sequences ◽  
Content Type ◽  
Clinical Strains

Staphylococcus aureus is a major cause of skin and soft tissue infections as well as bloodstream infections worldwide. Here, we report the draft genome sequences of 18 deidentified S. aureus clinical strains collected from positive blood cultures.

scholarly journals Molecular Types of Methicillin-Resistant Staphylococcus aureus and Methicillin-Sensitive S. aureus Strains Causing Skin and Soft Tissue Infections and Nasal Colonization, Identified in Community Health Centers in New York City

2015 ◽  
Vol 53 (8) ◽  
pp. 2648-2658 ◽  
Author(s):  
Maria Pardos de la Gandara ◽  
Juan Antonio Raygoza Garay ◽  
Michael Mwangi ◽  
Jonathan N. Tobin ◽  
Amanda Tsang ◽  
...  
Keyword(s):  
New York ◽  
Staphylococcus Aureus ◽  
New York City ◽  
Soft Tissue ◽  
York City ◽  
Community Health Centers ◽  
Health Centers ◽  
Soft Tissue Infections ◽  
Content Type ◽  
Clonal Type

In November 2011, The Rockefeller University Center for Clinical and Translational Science (CCTS), the Laboratory of Microbiology and Infectious Diseases, and Clinical Directors Network (CDN) launched a research and learning collaborative project with six community health centers in the New York City metropolitan area to determine the nature (clonal type) of community-acquiredStaphylococcus aureusstrains causing skin and soft tissue infections (SSTIs). Between November 2011 and March 2013, wound and nasal samples from 129 patients with active SSTIs suspicious forS. aureuswere collected and characterized by molecular typing techniques. In 63 of 129 patients, the skin wounds were infected byS. aureus: methicillin-resistantS. aureus(MRSA) was recovered from 39 wounds and methicillin-sensitiveS. aureus(MSSA) was recovered from 24. Most—46 of the 63–wound isolates belonged to the CC8/Panton-Valentine leukocidin-positive (PVL+) group ofS. aureusclone USA300: 34 of these strains were MRSA and 12 were MSSA. Of the 63 patients withS. aureusinfections, 30 were also colonized byS. aureusin the nares: 16 of the colonizing isolates were MRSA, and 14 were MSSA, and the majority of the colonizing isolates belonged to the USA300 clonal group. In most cases (70%), the colonizing isolate belonged to the same clonal type as the strain involved with the infection. In three of the patients, the identity of invasive and colonizing MRSA isolates was further documented by whole-genome sequencing.

scholarly journals Cold Atmospheric Plasma Promotes Killing of Staphylococcus aureus by Macrophages

mSphere ◽  
2021 ◽  
Author(s):  
Constance Duchesne ◽  
Nadira Frescaline ◽  
Océane Blaise ◽  
Jean-Jacques Lataillade ◽  
Sébastien Banzet ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Soft Tissue ◽  
Atmospheric Plasma ◽  
Soft Tissue Infections ◽  
Content Type ◽  
Cold Atmospheric Plasma ◽  
Resistant Strains

Staphylococcus aureus is the most frequent cause of skin and soft tissue infections. Treatment failures are increasingly common due to antibiotic resistance and the emergence of resistant strains.

scholarly journals Mupirocin Resistance in Staphylococcus aureus Causing Recurrent Skin and Soft Tissue Infections in Children

2011 ◽  
Vol 55 (5) ◽  
pp. 2431-2433 ◽  
Author(s):  
J. Chase McNeil ◽  
Kristina G. Hulten ◽  
Sheldon L. Kaplan ◽  
Edward O. Mason
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Trna Synthetase ◽  
Soft Tissue Infections ◽  
Methicillin Resistant ◽  
Content Type ◽  
Plasmid Gene ◽  
Children's Hospital ◽  
Children’S Hospital ◽  
Mupirocin Resistance

ABSTRACTStaphylococcus aureusresistance to mupirocin is often caused by acquisition of a novel isoleucyl-tRNA synthetase encoded on the plasmid genemupA. We testedS. aureusisolates from children at Texas Children's Hospital with recurrent skin and soft tissue infections for mupirocin resistance andmupA. Of 136 isolates, 20 were resistant to mupirocin (14.7%). Fifteen isolates (11%) carriedmupA, and the gene was more common in methicillin-susceptibleS. aureus(21.4%) than methicillin-resistantS. aureus(8.3%;P= 0.03). Seven of 20 mupirocin-resistant isolates displayed clindamycin resistance.

scholarly journals Efficacy of Lantibiotic Treatment of Staphylococcus aureus-Induced Skin Infections, Monitored byIn VivoBioluminescent Imaging

2016 ◽  
Vol 60 (7) ◽  
pp. 3948-3955 ◽  
Author(s):  
Anton Du Preez van Staden ◽  
Tiaan Heunis ◽  
Carine Smith ◽  
Shelly Deane ◽  
Leon M. T. Dicks
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Treatment Options ◽  
Bacterial Pathogen ◽  
Antimicrobial Activities ◽  
Skin Infections ◽  
Soft Tissue Infections ◽  
Content Type ◽  
Alternative Treatment Option

ABSTRACTStaphylococcus aureusis a bacterial pathogen responsible for the majority of skin and soft tissue infections. Antibiotics are losing their efficacy as treatment for skin and soft tissue infections as a result of increased resistance in a variety of pathogens, includingS. aureus. It is thus imperative to explore alternative antimicrobial treatments to ensure future treatment options for skin and soft tissue infections. A select few lantibiotics, a group of natural defense peptides produced by bacteria, inhibit the growth of numerous clinicalS. aureusisolates, including methicillin-resistant strains. In this study, the antimicrobial activities of nisin, clausin, and amyloliquecidin, separately administered, were compared to that of a mupirocin-based ointment, which is commonly used as treatment forS. aureus-induced skin infections. Full-thickness excisional wounds, generated on the dorsal surfaces of mice, were infected with a bioluminescent strain ofS. aureus(strain Xen 36). The infections were monitored in real time usingin vivobioluminescent imaging. Lantibiotic treatments significantly reduced the bioluminescence ofS. aureusXen 36 to a level similar to that recorded with mupirocin treatment. Wound closure, however, was more pronounced during lantibiotic treatment. Lantibiotics thus have the potential to be used as an alternative treatment option forS. aureus-induced skin infections.

scholarly journals Comparative Exoproteomics and Host Inflammatory Response in Staphylococcus aureus Skin and Soft Tissue Infections, Bacteremia, and Subclinical Colonization

2015 ◽  
Vol 22 (5) ◽  
pp. 593-603 ◽  
Author(s):  
Yun Khoon Liew ◽  
Rukman Awang Hamat ◽  
Alex van Belkum ◽  
Pei Pei Chong ◽  
Vasanthakumari Neela
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Elongation Factor ◽  
Soft Tissue Infections ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Cysteine Synthase ◽  
Chemokine Production ◽  
Content Type ◽  
Potential Biomarker

ABSTRACTThe exoproteome ofStaphylococcus aureuscontains enzymes and virulence factors that are important for host adaptation. We investigated the exoprotein profiles and cytokine/chemokine responses obtained in three differentS. aureus-host interaction scenarios by using two-dimensional gel electrophoresis (2-DGE) and two-dimensional immunoblotting (2D-IB) combined with tandem mass spectrometry (MS/MS) and cytometric bead array techniques. The scenarios includedS. aureusbacteremia, skin and soft tissue infections (SSTIs), and healthy carriage. By the 2-DGE approach, 12 exoproteins (the chaperone protein DnaK, a phosphoglycerate kinase [Pgk], the chaperone GroEL, a multisensor hybrid histidine kinase, a 3-methyl-2-oxobutanoate hydroxymethyltransferase [PanB], cysteine synthase A, anN-acetyltransferase, four isoforms of elongation factor Tu [EF-Tu], and one signature protein spot that could not be reliably identified by MS/MS) were found to be consistently present in more than 50% of the bacteremia isolates, while none of the SSTI or healthy-carrier isolates showed any of these proteins. By the 2D-IB approach, we also identified five antigens (methionine aminopeptidase [MetAPs], exotoxin 15 [Set15], a peptidoglycan hydrolase [LytM], an alkyl hydroperoxide reductase [AhpC], and a haptoglobin-binding heme uptake protein [HarA]) specific for SSTI cases. Cytokine and chemokine production varied during the course of different infection types and carriage. Monokine induced by gamma interferon (MIG) was more highly stimulated in bacteremia patients than in SSTI patients and healthy carriers, especially during the acute phase of infection. MIG could therefore be further explored as a potential biomarker of bacteremia. In conclusion, 12 exoproteins from bacteremia isolates, MIG production, and five antigenic proteins identified during SSTIs should be further investigated for potential use as diagnostic markers.

scholarly journals Origin and Evolution of European Community-Acquired Methicillin-Resistant Staphylococcus aureus

mBio ◽  
2014 ◽  
Vol 5 (5) ◽  
Author(s):  
Marc Stegger ◽  
Thierry Wirth ◽  
Paal S. Andersen ◽  
Robert L. Skov ◽  
Anna De Grassi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Middle East ◽  
North Africa ◽  
Fusidic Acid ◽  
Sub Saharan Africa ◽  
Soft Tissue Infections ◽  
Methicillin Resistant ◽  
Content Type ◽  
Sub Saharan

ABSTRACT Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was recognized in Europe and worldwide in the late 1990s. Within a decade, several genetically and geographically distinct CA-MRSA lineages carrying the small SCCmec type IV and V genetic elements and the Panton-Valentine leukocidin (PVL) emerged around the world. In Europe, the predominant CA-MRSA strain belongs to clonal complex 80 (CC80) and is resistant to kanamycin/amikacin and fusidic acid. CC80 was first reported in 1993 but was relatively rare until the late 1990s. It has since been identified throughout North Africa, the Middle East, and Europe, with recent sporadic reports in sub-Saharan Africa. While strongly associated with skin and soft tissue infections, it is rarely found among asymptomatic carriers. Methicillin-sensitive S. aureus (MSSA) CC80 strains are extremely rare except in sub-Saharan Africa. In the current study, we applied whole-genome sequencing to a global collection of both MSSA and MRSA CC80 isolates. Phylogenetic analyses strongly suggest that the European epidemic CA-MRSA lineage is derived from a PVL-positive MSSA ancestor from sub-Saharan Africa. Moreover, the tree topology suggests a single acquisition of both the SCCmec element and a plasmid encoding the fusidic acid resistance determinant. Four canonical SNPs distinguish the derived CA-MRSA lineage and include a nonsynonymous mutation in accessory gene regulator C (agrC). These changes were associated with a star-like expansion into Europe, the Middle East, and North Africa in the early 1990s, including multiple cases of cross-continent imports likely driven by human migrations. IMPORTANCE With increasing levels of CA-MRSA reported from most parts of the Western world, there is a great interest in understanding the origin and factors associated with the emergence of these epidemic lineages. To trace the origin, evolution, and dissemination pattern of the European CA-MRSA clone (CC80), we sequenced a global collection of strains of the S. aureus CC80 lineage. Our study determined that a single descendant of a PVL-positive methicillin-sensitive ancestor circulating in sub-Saharan Africa rose to become the dominant CA-MRSA clone in Europe, the Middle East, and North Africa. In the transition from a methicillin-susceptible lineage to a successful CA-MRSA clone, it simultaneously became resistant to fusidic acid, a widely used antibiotic for skin and soft tissue infections, thus demonstrating the importance of antibiotic selection in the success of this clone. This finding furthermore highlights the significance of horizontal gene acquisitions and underscores the combined importance of these factors for the success of CA-MRSA.

scholarly journals Mupirocin and Chlorhexidine Resistance in Staphylococcus aureus in Patients with Community-Onset Skin and Soft Tissue Infections

2012 ◽  
Vol 57 (1) ◽  
pp. 559-568 ◽  
Author(s):  
Stephanie A. Fritz ◽  
Patrick G. Hogan ◽  
Bernard C. Camins ◽  
Ali J. Ainsworth ◽  
Carol Patrick ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Routine Care ◽  
Soft Tissue Infections ◽  
Content Type ◽  
Before And After ◽  
Intranasal Mupirocin ◽  
Mupirocin Resistance ◽  
High Level ◽  
Community Onset

ABSTRACTDecolonization measures, including mupirocin and chlorhexidine, are often prescribed to preventStaphylococcus aureusskin and soft tissue infections (SSTI). The objective of this study was to determine the prevalence of high-level mupirocin and chlorhexidine resistance inS. aureusstrains recovered from patients with SSTI before and after mupirocin and chlorhexidine administration and to determine whether carriage of a mupirocin- or chlorhexidine-resistant strain at baseline precludedS. aureuseradication. We recruited 1,089 patients with community-onset SSTI with or withoutS. aureuscolonization. In addition to routine care, 483 patients were enrolled in a decolonization trial: 408 received intranasal mupirocin (with or without antimicrobial baths), and 258 performed chlorhexidine body washes. Patients were followed for up to 12 months with repeat colonization cultures. AllS. aureusisolates were tested for high-level mupirocin and chlorhexidine resistance. At baseline, 23/1,089 (2.1%) patients carried a mupirocin-resistantS. aureusstrain and 10/1,089 (0.9%) patients carried chlorhexidine-resistantS. aureus. Of 4 patients prescribed mupirocin, who carried a mupirocin-resistantS. aureusstrain at baseline, 100% remained colonized at 1 month compared to 44% of the 324 patients without mupirocin resistance at baseline (P= 0.041). Of 2 patients prescribed chlorhexidine, who carried a chlorhexidine-resistantS. aureusstrain at baseline, 50% remained colonized at 1 month compared to 48% of the 209 patients without chlorhexidine resistance at baseline (P= 1.0). The overall prevalence of mupirocin and chlorhexidine resistance is low inS. aureusisolates recovered from outpatients, but eradication efforts were less successful in patients carrying a mupirocin-resistantS. aureusstrain at baseline.

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