scholarly journals Convergence of TOR-Nitrogen and Snf1-Glucose Signaling Pathways onto Gln3

2002 ◽  
Vol 22 (4) ◽  
pp. 1246-1252 ◽  
Author(s):  
Paula G. Bertram ◽  
Jae H. Choi ◽  
John Carvalho ◽  
Ting-Fung Chan ◽  
Wandong Ai ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Glucose Sensor ◽  
Nitrogen Sources ◽  
Nutrient Sensing ◽  
Nutrient Sources ◽  
Glucose Signaling ◽  
Carbon And Nitrogen ◽  
Dependent Protein ◽  
Yeast Homolog

ABSTRACT Carbon and nitrogen are two basic nutrient sources for cellular organisms. They supply precursors for energy metabolism and metabolic biosynthesis. In the yeast Saccharomyces cerevisiae, distinct sensing and signaling pathways have been described that regulate gene expression in response to the quality of carbon and nitrogen sources, respectively. Gln3 is a GATA-type transcription factor of nitrogen catabolite-repressible (NCR) genes. Previous observations indicate that the quality of nitrogen sources controls the phosphorylation and cytoplasmic retention of Gln3 via the target of rapamycin (TOR) protein. In this study, we show that glucose also regulates Gln3 phosphorylation and subcellular localization, which is mediated by Snf1, the yeast homolog of AMP-dependent protein kinase and a cytoplasmic glucose sensor. Our data show that glucose and nitrogen signaling pathways converge onto Gln3, which may be critical for both nutrient sensing and starvation responses.

scholarly journals The effect of culture conditions for the mycelium growth of Phillenus linteus

2021 ◽  
Vol 63 (2) ◽  
pp. 39-43
Author(s):  
Van Giang Nguyen ◽  
◽  
Thi Bich Thuy Nguyen ◽  
Thi Khanh Linh Vu ◽  
Duy Trinh Nguyen ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Experimental Results ◽  
Phellinus Linteus ◽  
Mycelium Growth ◽  
Nutrient Sources ◽  
Carbon And Nitrogen ◽  
Medicinal Value

Phellinus linteus(Berk. & Curt.) Teng mushroom belonging to the genus Phellinusspp., has high medicinal value. This study was carried out with the aim to evaluate the effects of carbon, nitrogen sources, pH, and temperature on the mycelial growth of the P. linteusstrain. The experimental results showed that glucose and casein with concentrations of 2.0 and 0.2% respectively are identified as the most suitable carbon and nitrogen nutrient sources forP. linteus. Conversely, the mycelia of P. linteusare not able to grow on the urea media. Mycelium of P. linteus growths well at 7-10 pH and temperature at 30oC. When mycelia are incubated in these conditions, the rate of mycelial growth of P. linteus reaches 5.0 mm/day with thick density even distribution, and adherence to the substrate firmly.

Testing carbon and nitrogen sources for the in vitro growth of the model lichenized fungus Endocarpon pusillum Hedw.

2021 ◽  
Vol 53 (3) ◽  
pp. 257-264
Author(s):  
Oliver L. Mead ◽  
Cécile Gueidan
Keyword(s):  
Carbon Sources ◽  
Nitrogen Sources ◽  
Available Nitrogen ◽  
Primary Metabolite ◽  
Nutrient Sources ◽  
Future Studies ◽  
Carbon And Nitrogen ◽  
Growth Assay ◽  
Lichenized Fungus

AbstractTo improve the efficiency of isolating and culturing lichen mycobionts, we performed a growth assay on an Australian strain of the soil-crust lichenized fungus Endocarpon pusillum Hedw. This assay determined the preferred nitrogen and carbon sources of the fungus by limiting the available nitrogen or carbon sources to single compounds found in soils, plants and lichen thalli. We found that the non-proteinaceous amino acid, GABA, produced the most growth of all nutrients when provided as the sole nitrogen source but was a poor carbon source. Fructose, glucose, cellobiose and sorbitol produced the most growth of all the carbon sources tested. Ammonium, nitrate and polyamines were poor nutrient sources. These findings correspond with reports of primary metabolite pools in other lichen species and may guide future studies involving growth of recalcitrant lichen mycobionts.

Stable carbon and nitrogen isotopic characterization and tracing nutrient sources of Ulva blooms around Jeju coastal areas

2019 ◽  
Vol 254 ◽  
pp. 113033 ◽  
Author(s):  
Palas Samanta ◽  
Sookkyung Shin ◽  
Sojin Jang ◽  
Young-Cheol Song ◽  
Sangsil Oh ◽  
...  
Keyword(s):  
Coastal Areas ◽  
Stable Carbon ◽  
Nutrient Sources ◽  
Carbon And Nitrogen ◽  
Isotopic Characterization

scholarly journals A highly thermotolerant laccase produced by Cerrena unicolor strain CGMCC 5.1011 for complete and stable malachite green decolorization

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanhua Yao ◽  
Guimei Zhou ◽  
Yonghui Lin ◽  
Xinqi Xu ◽  
Jie Yang
Keyword(s):  
Malachite Green ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Life Time ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Carbon And Nitrogen ◽  
Cdna Sequences ◽  
Cerrena Unicolor ◽  
Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong
Keyword(s):  
Enhanced Oil Recovery ◽  
Bacterial Growth ◽  
Oil Recovery ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

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