Differential expression of the ras gene family in mice

1987 ◽  
Vol 7 (4) ◽  
pp. 1535-1540 ◽  
Author(s):  
J Leon ◽  
I Guerrero ◽  
A Pellicer
Keyword(s):  
Gene Family ◽  
Differential Expression ◽  
Adult Mouse ◽  
Expression Patterns ◽  
Cellular Functions ◽  
Ras Gene ◽  
Ras Genes ◽  
Mouse Tissues

We compared the expression of the ras gene family (H-ras, K-ras, and N-ras) in adult mouse tissues and during development. We found substantial variations in expression among different organs and in the amounts of the different transcripts originating from each gene, especially for the N-ras gene. The expression patterns were consistent with the reported preferential tissue activation of ras genes and suggested different cellular functions for each of the ras genes.

scholarly journals Differential expression of the ras gene family in mice.

1987 ◽  
Vol 7 (4) ◽  
pp. 1535-1540 ◽  
Author(s):  
J Leon ◽  
I Guerrero ◽  
A Pellicer
Keyword(s):  
Gene Family ◽  
Differential Expression ◽  
Adult Mouse ◽  
Expression Patterns ◽  
Cellular Functions ◽  
Ras Gene ◽  
Ras Genes ◽  
Mouse Tissues

We compared the expression of the ras gene family (H-ras, K-ras, and N-ras) in adult mouse tissues and during development. We found substantial variations in expression among different organs and in the amounts of the different transcripts originating from each gene, especially for the N-ras gene. The expression patterns were consistent with the reported preferential tissue activation of ras genes and suggested different cellular functions for each of the ras genes.

scholarly journals Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice

2011 ◽  
Vol 193 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Shinichi Nakagawa ◽  
Takao Naganuma ◽  
Go Shioi ◽  
Tetsuro Hirose
Keyword(s):  
Adult Mouse ◽  
Expression Patterns ◽  
Physiological Role ◽  
Growth Conditions ◽  
Nuclear Bodies ◽  
Cellular Functions ◽  
Mouse Tissues

Nuclei of higher organisms are well structured and have multiple, distinct nuclear compartments or nuclear bodies. Paraspeckles are recently identified mammal-specific nuclear bodies ubiquitously found in most cells cultured in vitro. To investigate the physiological role of paraspeckles, we examined the in vivo expression patterns of two long noncoding RNAs, NEAT1_1 and NEAT1_2, which are essential for the architectural integrity of nuclear bodies. Unexpectedly, these genes were only strongly expressed in a particular subpopulation of cells in adult mouse tissues, and prominent paraspeckle formation was observed only in the cells highly expressing NEAT1_2. To further investigate the cellular functions of paraspeckles, we created an animal model lacking NEAT1 by gene targeting. These knockout mice were viable and fertile under laboratory growth conditions, showing no apparent phenotypes except for the disappearance of paraspeckles. We propose that paraspeckles are nonessential, subpopulation-specific nuclear bodies formed secondary to particular environmental triggers.

Saccharomyces cerevisiae synthesizes proteins related to the p21 gene product of ras genes found in mammals

1984 ◽  
Vol 4 (1) ◽  
pp. 23-29
Author(s):  
A G Papageorge ◽  
D Defeo-Jones ◽  
P Robinson ◽  
G Temeles ◽  
E M Scolnick
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Family ◽  
Yeast Cells ◽  
Drosophila Genome ◽  
Ras Gene ◽  
Rat Serum ◽  
Viral Oncogenes ◽  
Ras Genes ◽  
Family Based ◽  
Related Proteins

A family of normal vertebrate genes and oncogenes has been called the ras gene family. The name ras was assigned to this gene family based on the species of origin of the viral oncogenes of the rat-derived Harvey and Kirsten murine sarcoma viruses. There are now three known functional members of the ras gene family, and genes homologous to ras genes have been detected in the DNA of a wide variety of mammals and in Drosophila melanogaster. Prior experiments have detected proteins coded for by ras genes in a large number of normal cells, cell lines, and tumors. We report here the detection of ras-related proteins in D. melanogaster, a result predicted by the earlier detection of ras-related genes in the Drosophila genome. We also report for the first time the detection of ras-related proteins in a single-cell eucaryocyte, Saccharomyces cerevisiae. These proteins, approximately 30K in size, are recognized by both a monoclonal antibody which binds to the p21 coded for by mammalian ras genes and a polyclonal rat serum made by transplanting a v-Ha-ras-induced tumor in Osborne-Mendel rats. The p21 of v-Ha-ras and the 30K proteins from S. cerevisiae share methionine-labeled peptides as detected by two-dimensional tryptic peptide maps. The results indicate that S. cerevisiae synthesizes ras-related proteins. A genetic analysis of the function of these proteins for yeast cells may now be possible.

scholarly journals Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish

2017 ◽  
Vol 114 (5) ◽  
pp. E717-E726 ◽  
Author(s):  
Jeremy S. Rabinowitz ◽  
Aaron M. Robitaille ◽  
Yuliang Wang ◽  
Catherine A. Ray ◽  
Ryan Thummel ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Amino Acid ◽  
Differential Expression ◽  
Spatial Information ◽  
Expression Patterns ◽  
Spatial Position ◽  
Adult Zebrafish ◽  
Cellular Functions ◽  
Caudal Fin ◽  
Functional Studies

Regeneration requires cells to regulate proliferation and patterning according to their spatial position. Positional memory is a property that enables regenerating cells to recall spatial information from the uninjured tissue. Positional memory is hypothesized to rely on gradients of molecules, few of which have been identified. Here, we quantified the global abundance of transcripts, proteins, and metabolites along the proximodistal axis of caudal fins of uninjured and regenerating adult zebrafish. Using this approach, we uncovered complex overlapping expression patterns for hundreds of molecules involved in diverse cellular functions, including development, bioelectric signaling, and amino acid and lipid metabolism. Moreover, 32 genes differentially expressed at the RNA level had concomitant differential expression of the encoded proteins. Thus, the identification of proximodistal differences in levels of RNAs, proteins, and metabolites will facilitate future functional studies of positional memory during appendage regeneration.

scholarly journals The tweety Gene Family: From Embryo to Disease

2021 ◽  
Vol 14 ◽  
Author(s):  
Rithvik R. Nalamalapu ◽  
Michelle Yue ◽  
Aaron R. Stone ◽  
Samantha Murphy ◽  
Margaret S. Saha
Keyword(s):  
Gene Family ◽  
Neural Development ◽  
Chloride Channels ◽  
Expression Patterns ◽  
Pediatric Brain Tumors ◽  
Structure Evolution ◽  
Cellular Functions ◽  
Stem Cell Maintenance ◽  
Embryonic And Fetal Development ◽  
Wide Range

The tweety genes encode gated chloride channels that are found in animals, plants, and even simple eukaryotes, signifying their deep evolutionary origin. In vertebrates, the tweety gene family is highly conserved and consists of three members—ttyh1, ttyh2, and ttyh3—that are important for the regulation of cell volume. While research has elucidated potential physiological functions of ttyh1 in neural stem cell maintenance, proliferation, and filopodia formation during neural development, the roles of ttyh2 and ttyh3 are less characterized, though their expression patterns during embryonic and fetal development suggest potential roles in the development of a wide range of tissues including a role in the immune system in response to pathogen-associated molecules. Additionally, members of the tweety gene family have been implicated in various pathologies including cancers, particularly pediatric brain tumors, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. Here, we review the current state of research using information from published articles and open-source databases on the tweety gene family with regard to its structure, evolution, expression during development and adulthood, biochemical and cellular functions, and role in human disease. We also identify promising areas for further research to advance our understanding of this important, yet still understudied, family of genes.

Differential Expression Patterns Within the Grapevine Stilbene Synthase Gene Family Revealed Through Their Regulatory Regions

2018 ◽  
Vol 36 (2) ◽  
pp. 225-238 ◽  
Author(s):  
Constanza Chialva ◽  
Claudio Muñoz ◽  
María Miccono ◽  
Estefanía Eichler ◽  
Luciano Calderón ◽  
...  
Keyword(s):  
Gene Family ◽  
Differential Expression ◽  
Expression Patterns ◽  
Stilbene Synthase ◽  
Regulatory Regions ◽  
Stilbene Synthase Gene

scholarly journals Expression of HSP86 in male germ cells.

1990 ◽  
Vol 10 (6) ◽  
pp. 3239-3242 ◽  
Author(s):  
S J Lee
Keyword(s):  
Germ Cell ◽  
Mrna Expression ◽  
Germ Cells ◽  
Adult Mouse ◽  
Expression Patterns ◽  
Testicular Function ◽  
Male Germ Cells ◽  
Homologous Genes ◽  
Cell Specificity ◽  
Mouse Tissues

A comparison of HSP84 and HSP86 mRNA expression in adult mouse tissues revealed distinct expression patterns for these highly homologous genes. Particularly striking is the germ cell specificity of HSP86 expression in the testis, suggesting distinct roles for HSP84 and HSP86 with respect to testicular function and development.

scholarly journals Saccharomyces cerevisiae synthesizes proteins related to the p21 gene product of ras genes found in mammals.

1984 ◽  
Vol 4 (1) ◽  
pp. 23-29 ◽  
Author(s):  
A G Papageorge ◽  
D Defeo-Jones ◽  
P Robinson ◽  
G Temeles ◽  
E M Scolnick
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Family ◽  
Yeast Cells ◽  
Drosophila Genome ◽  
Ras Gene ◽  
Rat Serum ◽  
Viral Oncogenes ◽  
Ras Genes ◽  
Family Based ◽  
Related Proteins

A family of normal vertebrate genes and oncogenes has been called the ras gene family. The name ras was assigned to this gene family based on the species of origin of the viral oncogenes of the rat-derived Harvey and Kirsten murine sarcoma viruses. There are now three known functional members of the ras gene family, and genes homologous to ras genes have been detected in the DNA of a wide variety of mammals and in Drosophila melanogaster. Prior experiments have detected proteins coded for by ras genes in a large number of normal cells, cell lines, and tumors. We report here the detection of ras-related proteins in D. melanogaster, a result predicted by the earlier detection of ras-related genes in the Drosophila genome. We also report for the first time the detection of ras-related proteins in a single-cell eucaryocyte, Saccharomyces cerevisiae. These proteins, approximately 30K in size, are recognized by both a monoclonal antibody which binds to the p21 coded for by mammalian ras genes and a polyclonal rat serum made by transplanting a v-Ha-ras-induced tumor in Osborne-Mendel rats. The p21 of v-Ha-ras and the 30K proteins from S. cerevisiae share methionine-labeled peptides as detected by two-dimensional tryptic peptide maps. The results indicate that S. cerevisiae synthesizes ras-related proteins. A genetic analysis of the function of these proteins for yeast cells may now be possible.

scholarly journals Targeted Long-Read Sequencing Decodes the Transcriptional Atlas of the Founding RAS Gene Family Members

2021 ◽  
Vol 22 (24) ◽  
pp. 13298
Author(s):  
Panagiotis G. Adamopoulos ◽  
Panagiotis Tsiakanikas ◽  
Michaela A. Boti ◽  
Andreas Scorilas
Keyword(s):  
Gene Family ◽  
Expression Profiles ◽  
Protein Isoforms ◽  
Alternative Transcript ◽  
Transcriptional Level ◽  
Ras Gene ◽  
Ras Genes ◽  
Transcript Variants ◽  
Depth Analysis ◽  
Genes Encoding

The complicity of human RAS proteins in cancer is a well-documented fact, both due to the mutational hyperactivation of these GTPases and the overexpression of the genes encoding these proteins. Thus, it can be easily assumed that the study of RAS genes at the transcriptional and post-transcriptional level is of the utmost importance. Although previous research has shed some light on the basic mechanisms by which GTPases are involved in tumorigenesis, limited information is known regarding the transcriptional profile of the genes encoding these proteins. The present study highlights for the first time the wide spectrum of the mRNAs generated by the three most significant RAS genes (KRAS, NRAS and HRAS), providing an in-depth analysis of the splicing events and exon/intron boundaries. The implementation of a versatile, targeted nanopore-sequencing approach led to the identification of 39 novel RAS mRNA transcript variants and to the elucidation of their expression profiles in a broad panel of human cell lines. Although the present work unveiled multiple hidden aspects of the RAS gene family, further study is required to unravel the biological function of all the novel alternative transcript variants, as well as the putative protein isoforms.

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