scholarly journals Molecular Characteristics of IS 1216 Carrying Multidrug Resistance Gene Cluster in Serotype III/Sequence Type 19 Group B Streptococcus

mSphere ◽  
2021 ◽  
Author(s):  
Yong Zhi ◽  
Hyun Jung Ji ◽  
Jong Hyun Jung ◽  
Eui Baek Byun ◽  
Woo Sik Kim ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Bacterial Colonization ◽  
Group B Streptococcus ◽  
Sequence Type ◽  
Molecular Characteristics ◽  
Protein Vaccine ◽  
Multidrug Resistance Gene ◽  
Content Type ◽  
Vaccine Candidates ◽  
Group B

Most previously isolated group B streptococcus (GBS) strains express either the Srr1 or Srr2 glycoprotein, which plays an important role in bacterial colonization and invasion. These glycoproteins are potential protein vaccine candidates.

scholarly journals Whole-Genome Shotgun Sequence of Streptococcus agalactiae Sequence Type 176 Strain 3966RFQB from a Dairy Herd in Selangor, Malaysia

2019 ◽  
Vol 8 (6) ◽  
Author(s):  
A. Bashir ◽  
Z. Zunita ◽  
F. F. A. Jesse ◽  
S. Z. Ramanoon ◽  
M. L. Mohd-Azmi
Keyword(s):  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Bovine Mastitis ◽  
Dairy Herd ◽  
Sequence Type ◽  
Control Measures ◽  
Content Type ◽  
Whole Genome Shotgun Sequence ◽  
Genome Shotgun Sequence ◽  
Group B

Streptococcus agalactiae, commonly known as group B streptococcus (GBS), is among the most implicated pathogens in bovine mastitis worldwide. Proper control measures can curb both economic and public health effects it may cause.

scholarly journals Molecular Characteristics of Group B Streptococci Isolated from Adults with Invasive Infections in Japan

2016 ◽  
Vol 54 (11) ◽  
pp. 2695-2700 ◽  
Author(s):  
Miyuki Morozumi ◽  
Takeaki Wajima ◽  
Misako Takata ◽  
Satoshi Iwata ◽  
Kimiko Ubukata
Keyword(s):  
Amino Acid ◽  
Group B Streptococcus ◽  
Antibiotic Use ◽  
Molecular Characteristics ◽  
Amino Acid Substitutions ◽  
Group B Streptococci ◽  
Content Type ◽  
Invasive Infections ◽  
Primary Focus ◽  
Group B

Streptococcus agalactiae(group B streptococcus) isolates (n= 443) obtained from Japanese adults with invasive infections between April 2010 and March 2013 were analyzed for capsular serotype, multilocus sequence type (ST), antibiotic susceptibility, and resistance genes. Among these cases, bacteremia without primary focus was the most common variety of infection (49.9%), followed by cellulitis (12.9%) and pneumonia (9.0%). Concerning patient age (18 to 59, 60 to 69, 70 to 79, 80 to 89, and 90 years old or older), the incidence of pneumonia increased in patients in their 70s and 80s (P< 0.001), while younger patients (18 to 59 and 60 to 69 years old) were more likely to have abscesses (P< 0.05). The mortality rate was 10.2% for all ages. The most common capsular serotype was Ib (39.5%), followed by V (16.0%), III (13.8%), VI (9.5%), and Ia (8.6%). The main ST of serotype Ib strains was ST10, which belonged to clonal complex 10 (88.0%). The predominant clonal complexes of serotypes V and III, respectively, were 1 (78.9%) and 19 (75.4%). Among these isolates, 9 strains (2.0%) were identified as group B streptococci with reduced penicillin susceptibility, reflecting amino acid substitutions in penicillin-binding protein 2X (PBP2X). In addition, 19.2% of all strains possessedmef(A/E),erm(A), orerm(B) genes, which mediate macrolide resistance, while 40.2% of strains were resistant to quinolones resulting from amino acid substitutions in GyrA and ParC. Our data argue strongly for the continuous surveillance of microbial characteristics and judicious antibiotic use in clinical practice.

scholarly journals A Vaginal Tract Signal Detected by the Group B Streptococcus SaeRS System Elicits Transcriptomic Changes and Enhances Murine Colonization

2018 ◽  
Vol 86 (4) ◽  
Author(s):  
Laura C. C. Cook ◽  
Hong Hu ◽  
Mark Maienschein-Cline ◽  
Michael J. Federle
Keyword(s):  
Reproductive Tract ◽  
Bacterial Colonization ◽  
Group B Streptococcus ◽  
Lavage Fluid ◽  
Content Type ◽  
Colonization Factor ◽  
Vaginal Tract ◽  
Group B ◽  
Virulence Regulator

ABSTRACTStreptococcus agalactiae(group B streptococcus [GBS]) can colonize the human vaginal tract, leading to both superficial and serious infections in adults and neonates. To study bacterial colonization of the reproductive tract in a mammalian system, we employed a murine vaginal carriage model. Using transcriptome sequencing (RNA-Seq), the transcriptome of GBS growingin vivoduring vaginal carriage was determined. Over one-quarter of the genes in GBS were found to be differentially regulated duringin vivocolonization compared to laboratory cultures. A two-component system (TCS) homologous to the staphylococcal virulence regulator SaeRS was identified as being upregulatedin vivo. One of the SaeRS targets,pbsP, a proposed GBS vaccine candidate, is shown to be important for colonization of the vaginal tract. A component of vaginal lavage fluid acts as a signal to turn onpbsPexpression via SaeRS. These data demonstrate the ability to quantify RNA expression directly from the murine vaginal tract and identify novel genes involved in vaginal colonization by GBS. They also provide more information about the regulation of an important virulence and colonization factor of GBS,pbsP, by the TCS SaeRS.

scholarly journals Emergence of Serotype IV Group B Streptococcus Adult Invasive Disease in Manitoba and Saskatchewan, Canada, Is Driven by Clonal Sequence Type 459 Strains

2015 ◽  
Vol 53 (9) ◽  
pp. 2919-2926 ◽  
Author(s):  
Sarah Teatero ◽  
Taryn B. T. Athey ◽  
Paul Van Caeseele ◽  
Greg Horsman ◽  
David C. Alexander ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Group B Streptococcus ◽  
The United States ◽  
Invasive Disease ◽  
Sequence Type ◽  
Polymorphism Analysis ◽  
Invasive Infection ◽  
Content Type ◽  
Total Burden ◽  
Group B

Serotype IV group BStreptococcus(GBS) is emerging in Canada and the United States with rates as high as 5% of the total burden of adult invasive GBS disease. To understand this emergence, we studied the population structure and assessed the antimicrobial susceptibility of serotype IV isolates causing adult invasive infection in Manitoba and Saskatchewan, Canada, between 2010 and 2014. Whole-genome sequencing was used to determine multilocus sequence typing information and identify genes encoding antimicrobial resistance in 85 invasive serotype IV GBS strains. Antimicrobial susceptibility testing was performed by standard methods. Strain divergence was assessed using genome-wide single-nucleotide polymorphism analysis. Serotype IV strains were responsible for 16.9% of adult invasive GBS infections in Manitoba and Saskatchewan during the period. The majority of serotype IV isolates (89%) were clonally related, tetracycline-, erythromycin-, and clindamycin-resistant sequence type 459 (ST459) strains that possessed genestetMandermTR. Genome comparisons between ST459 and serotype V ST1 GBS identified several areas of recombination in an overall similar genomic background. Serotype IV ST459 GBS strains are expanding and causing a substantial percentage of adult invasive GBS disease. This emergence may be linked to the acquisition of resistance to tetracycline, macrolides, and lincosamides.

scholarly journals Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015–2020

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dusan Kekic ◽  
Ina Gajic ◽  
Natasa Opavski ◽  
Milan Kojic ◽  
Goran Vukotic ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Late Onset ◽  
Group B Streptococcus ◽  
Neonatal Morbidity ◽  
Disease Rate ◽  
Molecular Characteristics ◽  
Group B Streptococci ◽  
Live Births ◽  
Invasive Infections ◽  
Group B

AbstractGroup B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality. Serbia has not fully implemented preventive measures against GBS neonatal diseases. Therefore, we aimed to assess the maternal GBS colonisation and invasive neonatal disease rate, to reveal the trends of antimicrobial resistance and serotype distribution of GBS from various patient groups. Randomly selected non-invasive (n = 991) and all invasive GBS (n = 80) collected throughout Serbia from 2015 to 2020 were tested for antimicrobial susceptibility, capsular typing, and hvgA detection. Overall, 877/5621 (15.6%) pregnant women were colonised with GBS. Invasive GBS infections incidence in infants (0.18/1000 live births) showed a decreasing trend (0.3 to 0.1/1000 live births). Type III was overrepresented in infants with invasive infections (n = 35, 58.3%), whereas type V predominated among colonised adults (n = 224, 25.5%) and those with noninvasive (n = 37, 32.5%) and invasive infections (n = 8, 40%). The hypervirulent clone III/ST17 was highly associated with invasive infections (n = 28, 35%), particularly late-onset disease (n = 9, 47.4%), showing an increase from 12.3 to 14.8%. The GBS resistance to erythromycin and clindamycin was 26.7% and 22.1%, respectively, with an upward trend. The emergence of the hypervirulent clone III/ST17 and the escalation in GBS resistance highlight an urgent need for continuous monitoring of GBS infections.

scholarly journals Evaluation of StrepBSelectChromogenic Medium and the Fast-Track Diagnostics Group B Streptococcus (GBS) Real-Time PCR Assay Compared to Routine Culture for Detection of GBS during Antepartum Screening

2017 ◽  
Vol 55 (7) ◽  
pp. 2137-2142 ◽  
Author(s):  
Deirdre L. Church ◽  
Heather Baxter ◽  
Tracie Lloyd ◽  
Oscar Larios ◽  
Daniel B. Gregson
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Fast Track ◽  
Group B Streptococcus ◽  
Culture Method ◽  
Predictive Values ◽  
Content Type ◽  
Standard Culture ◽  
Group B ◽  
The Cost

ABSTRACTLife-threatening infection in neonates due to group BStreptococcus(GBS) is preventable by screening of near-term pregnant women and treatment at delivery. A total of 295 vaginal-rectal swabs were collected from women attending antepartum clinics in Calgary, Alberta, Canada. GBS colonization was detected by the standard culture method (Strep B Carrot Broth subcultured to blood agar with a neomycin disk) and compared to recovery with Strep Group B Broth (Dalynn Biologicals) subcultured to StrepBSelectchromogenic medium (CM; Bio-Rad Laboratories) and the Fast-Track Diagnostics GBS real-time PCR (quantitative PCR [qPCR]) assay (Phoenix Airmid Biomedical Corp.) performed with broth-enriched samples and the Abbottm2000sp/m2000rt system. A total of 62/295 (21%) women were colonized with GBS; 58 (19.7%) cases were detected by standard culture, while CM and qPCR each found 61 (20.7%) cases. The qPCR and CM were similar in performance, with sensitivities, specificities, and positive and negative predictive values of 98.4 and 98.4%, 99.6 and 99.6%, 98.4 and 98.4%, and 99.6 and 99.6%, respectively, compared to routine culture. Both qPCR and CM would allow more rapid reporting of routine GBS screening results than standard culture. Although the cost per test was similar for standard culture and CM, the routine use of qPCR would cost approximately four times as much as culture-based detection. Laboratories worldwide should consider implementing one of the newer methods for primary GBS testing, depending on the cost limitations of different health care jurisdictions.

scholarly journals Novel Plasmid-Borne Multidrug Resistance Gene Cluster Includinglsa(E) from a Linezolid-Resistant Enterococcus faecium Isolate of Swine Origin

2015 ◽  
Vol 59 (11) ◽  
pp. 7113-7116 ◽  
Author(s):  
Hongbin Si ◽  
Wan-Jiang Zhang ◽  
Shengbo Chu ◽  
Xiu-Mei Wang ◽  
Lei Dai ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Enterococcus Faecium ◽  
Insertion Element ◽  
Multidrug Resistance Gene ◽  
Content Type ◽  
Recombination Processes ◽  
Faecium Isolate

ABSTRACTA novel nonconjugative plasmid of 28,489 bp from a porcine linezolid-resistantEnterococcus faeciumisolate was completely sequenced. This plasmid harbored a novel type of multiresistance gene cluster that comprised the resistance geneslnu(B),lsa(E),spw,aadE,aphA3, and two copies oferm(B), which account for resistance to macrolides, lincosamides, streptogramins, pleuromutilins, streptomycin, spectinomycin, and kanamycin/neomycin. Structural comparisons suggested that this plasmid might have developed from other enterococcal plasmids by insertion element (IS)-mediated interplasmid recombination processes.

scholarly journals Whole-Genome Sequencing Confirms the Coexistence of Different Colonizing Group B Streptococcus Isolates Underscored by CRISPR Typing

2020 ◽  
Vol 9 (5) ◽  
Author(s):  
Clémence Beauruelle ◽  
Maxime Branger ◽  
Thierry Cochard ◽  
Adeline Pastuszka ◽  
Franck Biet ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Draft Genome ◽  
Group B Streptococcus ◽  
Whole Genome ◽  
Industrialized Countries ◽  
Genome Sequences ◽  
Neonatal Infections ◽  
Content Type ◽  
Phylogenetic Affiliation ◽  
Group B

Streptococcus agalactiae is a major pathogen and is the leading cause of neonatal infections in industrialized countries. The diversity of strains isolated from two pregnant women was investigated. Here, we present the draft genome sequences of strains W8A2, W8A6, W10E2, and W10F3, obtained in order to ascertain their phylogenetic affiliation.

scholarly journals Comparison of the Panther Fusion and BD MAX Group B Streptococcus (GBS) Assays for Detection of GBS in Prenatal Screening Specimens

2019 ◽  
Vol 57 (11) ◽  
Author(s):  
Gregory J. Berry ◽  
Fan Zhang ◽  
Ryhana Manji ◽  
Stefan Juretschko
Keyword(s):  
Late Onset ◽  
Clinical Performance ◽  
Group B Streptococcus ◽  
Turnaround Time ◽  
Nucleic Acid Amplification ◽  
Kappa Statistics ◽  
Loading Capacity ◽  
Content Type ◽  
Return Visits ◽  
Group B

ABSTRACT Streptococcus agalactiae or group B Streptococcus (GBS) is the cause of early- and late-onset GBS disease in neonates and can present as septicemia, meningitis, and pneumonia. Our objective was to compare the performance of two FDA-approved nucleic acid amplification tests (NAATs), the Panther Fusion and BD MAX systems, for detection of GBS in vaginal-rectal screening specimens. A total of 510 vaginal-rectal prepartum specimens were tested simultaneously in both NAATs following broth enrichment. Assay agreement was calculated using kappa statistics. Overall agreement between assays was 99.0% (505/510; 95% confidence interval, 0.951 to 0.997; kappa = 0.974). Discordant results were retested with both assays and by standard culture. The assays were also compared for workflow characteristics, including time to first results (TFR), total turnaround time (TAT), number of return visits to load additional specimens, and hands-on time (HoT). Using a standard run size of 60 specimens/day, the Panther Fusion assay had a longer TFR (2.4 versus 2.0 h) but showed a shorter overall TAT for all 60 samples (3.98 versus 7.18 h) due to an increased initial sample loading capacity, and it required less labor (35.0 versus 71.3 s/sample) and fewer return visits for loading additional specimens (0 versus 2). The Panther Fusion system also had a larger sample loading capacity (120 versus 24 samples) and greater 8-h throughput (335 versus 96 samples). In summary, the Panther Fusion GBS assay has clinical performance comparable to that of the BD MAX GBS assay but provides a faster TAT, less HoT, and higher throughput.

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