Specific features of temperature scalar field and tie-line vector field diagrams for three-component two-phase systems

2006 ◽  
Vol 40 (6) ◽  
pp. 604-610
Author(s):  
L. A. Serafimov ◽  
T. V. Chelyuskina ◽  
D. I. Bushina
Keyword(s):  
Vector Field ◽  
Scalar Field ◽  
Two Phase ◽  
Phase Systems ◽  
Tie Line ◽  
Temperature Scalar

scholarly journals Phase Separation Behavior and System Properties of Aqueous Two-Phase Systems with Polyethylene Glycol and Different Salts: Experiment and Correlation

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Haihua Yuan ◽  
Yang Liu ◽  
Wanqian Wei ◽  
Yongjie Zhao
Keyword(s):  
Phase Separation ◽  
Sodium Citrate ◽  
Line Length ◽  
Continuous Phase ◽  
Two Phase ◽  
Separation Rate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
System Properties ◽  
Tie Line

The phase separation behaviors of PEG1000/sodium citrate, PEG4000/sodium citrate, PEG1000/ammonium sulfate, and PEG4000/ammonium sulfate aqueous two-phase systems were investigated, respectively. There are two distinct situations for the phase separation rate in the investigated aqueous two-phase systems: one state is top-continuous phase with slow phase separation rate and strong bottom-continuous phase with fast phase separation rate and weak volume ratio dependence. The system properties such as density, viscosity, and interfacial tension between top and bottom phases which have effects on the phase separation rate of aqueous two-phase systems were measured. The property parameter differences between the two phases increased with increasing tie line length and then improved the phase separation rate. Moreover, a modified correlation equation including the phase separation rate, tie line length, and physical properties of the four aqueous two-phase systems has been proposed and successfully tested in the bottom-continuous phase, whose coefficients were estimated through regression analysis. The predicted results of PEG1000/sodium citrate aqueous two-phase systems were verified through the stationary phase retention in the cross-axis countercurrent chromatography.

Liquid-Liquid Equilibria for Acetone + Several Citrates Aqueous Two-Phase Systems at Different Temperatures

2012 ◽  
Vol 549 ◽  
pp. 30-35
Author(s):  
Shi Ping Hu ◽  
Juan Han ◽  
Yong Sheng Yan ◽  
Yu Tao Hu
Keyword(s):  
Ternary Systems ◽  
Data Fitting ◽  
Potassium Citrate ◽  
Liquid Equilibrium ◽  
Two Phase ◽  
Different Temperatures ◽  
Tie Lines ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Tie Line

Liquid-liquid equilibria for the three kinds of the ternary systems acetone + ammonium, sodium or potassium citrate + water have been determined at T= (273.15, 283.15, and 298.15) K. Binodal curves, tie-lines, and integrated phase diagrams for the ternary systems are given. The data of the experimental bimodal curve are described with a four-parameter equation. The result also shows the temperature has little influence on the liquid-liquid equilibrium within the investigated range. The tie-line data calculated according to the bimodal data fitting equation and the lever arm rule were satisfactorily described by using the Othmer-Tobias and Bancroft equations, and the result conform the reliability of the calculation method and corresponding tie-line data.

scholarly journals Liquid-Liquid Equilibrium Data for the Ionic Liquid N-Ethyl-Pyridinium Bromide with Several Sodium Salts and Potassium Salts

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Yuliang Li ◽  
Xiaojia Lu ◽  
Jing Hao ◽  
Changquan Chen
Keyword(s):  
Correlation Coefficients ◽  
Liquid Equilibrium ◽  
Two Phase ◽  
Salting Out ◽  
Pyridinium Bromide ◽  
Equilibrium Data ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Lle Data ◽  
Tie Line

The liquid-liquid equilibrium (LLE) data for systems containing N-ethyl-pyridinium bromide ([EPy]Br), salt (Na2HPO4,K2HPO4,K2SO4,C4O6H4KNa), and water have been measured experimentally atT=298.15 K and the formations of these four aqueous two-phase systems (ATPSs) have been discussed. Also, the effective excluded volume (EEV) values obtained from the binodal models for the four systems were determined and the salting-out abilities of different salts follow the order ofK2SO4>K2HPO4>Na2HPO4>C4O6H4KNa. The solubility data were correlated by the Merchuk and other equations while the tie-line data by the Othmer-Tobias, Bancroft, two-parameter, and Setschenow-type equations. The correlation coefficients evidenced that experimental data fitted well to all these equations. These four salts were proved successfully to form ATPSs with N-ethyl-pyridinium bromide, making a significant contribution to the further study of this kind of ATPS.

scholarly journals The effect of sulphates on partitioning of pectinases in aqueous two-phase systems

2004 ◽  
pp. 179-186 ◽  
Author(s):  
Mirjana Antov ◽  
Draginja Pericin ◽  
Biljana Trbojevic
Keyword(s):  
Polyethylene Glycol ◽  
Partition Coefficient ◽  
Ammonium Sulphate ◽  
Line Length ◽  
Sodium Sulphate ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Sulphate Salts ◽  
Tie Line

The effect of various sulphate salts on the partitioning of endo-pectinase and exo-pectinase in aqueous two-phase systems, composed of polyethylene glycol and dextran, was studied. Presence of ammonium sulphate and sodium sulphate at concentration 15 mmol/l in the system polyethylene glycol 4000/crude dextran, at tie-line length 7.44%, increased partition coefficient of endo-pectinase 1.25 and 1.2 fold, respectively. Ammonium sulphate at 15 mmol/l and sodium sulphate at 5 mmol/l enhanced partition coefficient for exo-pectinase for about 60% in comparison to the system without salts. Addition of magnesium and sodium sulphate to a final concentration of 0.3 mmol/l in the system containing polyethylene glycol 6000/dextran 500 000, at tie-line length 6.26%, increased the partition coefficient of endo activity for 95% and 32%, respectively. Both salts at the same concentration increased partition coefficient of exo activity 1.5 and 3 times, respectively, in comparison to the system without salts.

Cell Separation in Aqueous Two-Phase Systems − Influence of Polymer Molecular Weight and Tie-Line Length on the Resolution of Five Model Cell Lines

2017 ◽  
Vol 13 (2) ◽  
pp. 1700250 ◽  
Author(s):  
Sarah Zimmermann ◽  
Sarah Gretzinger ◽  
Philipp K. Zimmermann ◽  
Are Bogsnes ◽  
Mattias Hansson ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Lines ◽  
Cell Separation ◽  
Line Length ◽  
Polymer Molecular Weight ◽  
Two Phase ◽  
Model Cell ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Tie Line

Tie line framework to optimize non-enveloped virus recovery in aqueous two-phase systems

2019 ◽  
Vol 1126-1127 ◽  
pp. 121744 ◽  
Author(s):  
Pratik U. Joshi ◽  
Dylan G. Turpeinen ◽  
Matthew Weiss ◽  
Glendy Escalante-Corbin ◽  
Michael Schroeder ◽  
...  
Keyword(s):  
Two Phase ◽  
Enveloped Virus ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Virus Recovery ◽  
Tie Line

Studies on Aqueous Two Phase Polymer Systems Useful for Partitioning of Biological Materials

1981 ◽  
Vol 9 ◽  
Author(s):  
Donald E. Brooks ◽  
Stephan Bamberger
Keyword(s):  
Electric Fields ◽  
Critical Concentration ◽  
Drop Diameter ◽  
Electrokinetic Properties ◽  
Two Phase ◽  
Liquid Phase Boundary ◽  
Phase Systems ◽  
Cell Subpopulations ◽  
Poly Ethylene ◽  
Tie Line

ABSTRACTThe two phase systems that result when aqueous solutions of dextran and poly(ethylene glycol) (PEG) are mixed above a critical concentration of a few percent provide a useful medium for the separation of biological cell subpopulations via partition between the top, PEG-rich phase and the liquid-liquid phase boundary. Interfacial tensions of such systems have been measured by the rotating drop technique and found to range between 10−1 and 102 μN m−1 . The tension was found to depend on the length of the tie line describing the system on a phase diagram, via a power law relationship which differed depending on the concentration of Na phosphate buffer present. The electrokinetic properties of drops of one phase suspended in the other were studied for a variety of systems. It was found that the droplet electrophoretic mobility increased monotonically with phosphate concentration and drop diameter but exhibited the opposite sign from that anticipated from phosphate partition measurements. It was possible to take advantage of these electrokinetic properties and dramatically enhance the speed of phase separation through application of relatively small electric fields.

scholarly journals Effects of the polymer molecular weight and type of cation on phase diagrams of polythylene glycol + sulfate salts aqueous two-phase systems

2019 ◽  
Vol 73 (6) ◽  
pp. 375-385
Author(s):  
Gholamhossein Parmoon ◽  
Abdorreza Nafchi ◽  
Mohsen Pirdashti
Keyword(s):  
Molecular Weight ◽  
Magnesium Sulfate ◽  
Phase Diagrams ◽  
Zinc Sulfate ◽  
Two Phase ◽  
Aluminium Sulfate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Lle Data ◽  
Tie Line

Phase diagrams and liquid ? liquid equilibrium (LLE) data for aqueous two-phase systems (ATPSs) containing zinc sulfate, magnesium sulfate or aluminium sulfate and polyethylene glycols PEG 300, 400 and 600 have been determined at 298.15 K. It was attempted to show how the PEG molecular weight and the type of cation influence the binodal curve, tie line length (TLL) and slope of the tie line (STL). The results have shown that as the PEG molecular weight increases, the two-phase region becomes extended and the binodal curve shifts to the origin. The refractive index and density of ternary (PEG 300,400 and 600 + zinc sulfate/magnesium sulfate/aluminium sulfate + water) systems have been measured to achieve the phase composition and the tie lines. Finally, the effective excluded volume (EEV) model was applied to describe the salting-out ability of the systems. The LLE data from this research may be potentially used for recovering biological molecules like proteins.

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