Immbilization on cellulose in bead form after periodate oxidation and reductive alkylation

1979 ◽  
Vol 44 (11) ◽  
pp. 3411-3417 ◽  
Author(s):  
Jaroslava Turková ◽  
Jiří Vajčner ◽  
Drahomíra Vančurová ◽  
Jiří Štamberg
Keyword(s):  
Molecular Weight ◽  
Covalent Binding ◽  
Periodate Oxidation ◽  
Coupling Reaction ◽  
Low Molecular Weight ◽  
Cellulose Derivatives ◽  
Cellulose Oxidation ◽  
Immobilized Trypsin ◽  
The Stability ◽  
Hydrolysis Of

The covalent binding of trypsin to cellulose beads after periodate oxidation was examined. The degree of cellulose derivatives solubilization is directly related to cellulose oxidation and increases with the increasing pH of the reaction mixture. The quantity of trypsin immobilized was examined as a function of pH, reaction time, and concentration of native trypsin in the reaction mixture during the coupling reaction. The catalytic activity and the stability of the preparations of immobilized trypsin during continuous hydrolysis of low-molecular-weight and high-molecular-weight substrates was assayed.

scholarly journals An Improved Procedure for the Preparation of Thrombin Low Molecular Weight Substrates - Peptide p-Nitroanilides

2018 ◽  
Vol 1 (4) ◽  
pp. e00057 ◽  
Author(s):  
A.A Chistov ◽  
A.V. Talanova ◽  
M.V. Melnikova ◽  
S.S. Kuznetsova ◽  
E.F. Kolesanova
Keyword(s):  
Molecular Weight ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Potassium Sulfate ◽  
Polymer Support ◽  
Low Molecular Weight ◽  
Chromogenic Substrate ◽  
Peptide Substrates ◽  
Terminal Amino ◽  
Hydrolysis Of

Low molecular weight chromogenic thrombin peptide substrates, p-nitroanilides of short peptides protected at their N-terminal amino group, were prepared by solid-phase peptide synthesis on polystyrene-divinylbenzene polymer with trityl groups with preliminary attached p-phenylene diamine moiety. After the cleavage from the resin peptide p-aminoanilides were mildly oxidized to p-nitroanilides with the mixture of potassium sulfate and persulfate. Adsorption onto polymer support Bio-Beads SM-2 with further elution by acetonitrile allowed easy separating peptide p-nitroanilides from the oxidizer and obtaining the thrombin chromogenic substrate preparations with the target substance contents of not less than 95% and yields of 30-40%. Thrombin effectively catalyzed hydrolysis of the prepared substrates with KM and Vmax values of 29-134 mM and 0.03-1/16 mM/s, respectively.

scholarly journals Biodegradation of low molecular weight polyacrylamide under aerobic and anaerobic conditions: effect of the molecular weight

2020 ◽  
Vol 81 (2) ◽  
pp. 301-308 ◽  
Author(s):  
Wenzhe Song ◽  
Yu Zhang ◽  
Amir Hossein Hamidian ◽  
Min Yang
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Carbon Chain ◽  
Anaerobic Treatment ◽  
Low Molecular Weight ◽  
Amino Groups ◽  
Molecular Weights ◽  
Weight One ◽  
Hydrolysis Of ◽  
Aerobic And Anaerobic

Abstract The biodegradation of polyacrylamide (PAM) includes the hydrolysis of amino groups and cleavage of the carbon chain; however, the effect of molecular weight on the biodegradation needs further investigations. In this study, biodegradation of low molecular weight PAM (1.6 × 106 Da) was evaluated in two aerobic (25 °C and 40 °C) and two anaerobic (35 °C and 55 °C) reactors over 100 days. The removal of the low molecular weight PAM (52.0–52.6%) through the hydrolysis of amino groups by anaerobic treatment (35 °C and 55 °C) was much higher than that of the high molecular weight (2.2 × 107 Da, 11.2–17.0%) observed under the same conditions. The molecular weight was reduced from 1.6 × 106 to 6.45–7.42 × 105 Da for the low molecular weight PAM, while the high molecular weight PAM declined from 2.2 × 107 to 3.76–5.87 × 106 Da. The results showed that the amino hydrolysis of low molecular weight PAM is easier than that of the high molecular weight one, while the cleavage of its carbon chain is still difficult. The molecular weights of PAM in the effluents from the two aerobic reactors (25 °C and 40 °C) were further reduced to 4.31 × 105 and 5.68 × 105 Da by the biofilm treatment, respectively. The results would be useful for the management of wastewater containing PAM.

scholarly journals Effects Of Environmental Factors On The Dispersion Behavior Of Iron Oxide In Aqueous Solutions With Poly Acrylic Acid

2015 ◽  
Vol 60 (2) ◽  
pp. 1561-1564
Author(s):  
E.-H. Lee ◽  
K.-M. Kim ◽  
W.-Y. Maeng ◽  
D.-H. Hur
Keyword(s):  
Molecular Weight ◽  
Acrylic Acid ◽  
High Molecular Weight ◽  
Low Molecular Weight ◽  
Solution Ph ◽  
Molecular Weights ◽  
Aqueous Suspensions ◽  
Magnetite Particles ◽  
The Stability ◽  
Poly Acrylic Acid

Abstract After preparing aqueous suspensions from magnetite particles with a poly-acrylic acid, we investigated the effects of several experimental parameters. We characterized the stability of the suspensions using visual inspection, sedimentation, adsorption, and thermal stability of the dispersant. The dispersion stability is affected by the solution pH, the concentrations of magnetite particles, the molecular weight, the concentration of the dispersants, and the temperature. The stability of the suspensions increased as the concentration of the dispersant and the temperature increased. In terms of the molecular weights of the dispersant, the suspensions with dispersant of low-molecular weight (1800) were more stable than those of high-molecular weight (250000) at room temperature. However, at high temperature the suspensions with high-molecular weight showed stability. The adsorption efficiency of the dispersant was very low. The dispersant of high-molecular weight showed a higher thermal integrity than that of low-molecular weight. From this work, we obtained the optimum conditions for stable aqueous suspensions of magnetite particles.

Detection of cellulase inhibitor in the wheat bran culture of Aspergillus terreus

1981 ◽  
Vol 27 (12) ◽  
pp. 1334-1340 ◽  
Author(s):  
S. N. Sinha ◽  
B. L. Ghosh ◽  
S. N. Ghose
Keyword(s):  
Molecular Weight ◽  
Wheat Bran ◽  
Filter Paper ◽  
Aspergillus Terreus ◽  
Defined Medium ◽  
Low Molecular Weight ◽  
Chemically Defined Medium ◽  
Plant Origin ◽  
First Time ◽  
Hydrolysis Of

The presence of a cellulase inhibitor in the wheat bran culture of a fungus is reported for the first time. The inhibitor has a low molecular weight and is relatively stable to heat. It is absent from wheat bran and is not produced in a chemically defined medium. Unlike cellulase inhibitors of plant origin, this inhibitor is not a polyphenol. It inhibits the hydrolysis of cotton to a greater degree than that of filter paper or carboxymethylcellulose. In addition to inhibiting Aspergillus terreus cellulase, it also inhibits a variety of commercial cellulases.

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