Synthesis and biochemical properties of the novel, enzymatically stable mRNA cap analogues with versatile potential applications

2005 ◽  
Author(s):  
Marcin Kalek ◽  
Jacek Jemielity ◽  
Ewa Grudzien ◽  
Joanna Zuberek ◽  
Zbigniew M. Darzynkiewicz ◽  
...  
Keyword(s):  
Biochemical Properties ◽  
The Novel ◽  
Potential Applications

scholarly journals Influence of protein (human galectin-3) design on aspects of lectin activity

2020 ◽  
Vol 154 (2) ◽  
pp. 135-153 ◽  
Author(s):  
Gabriel García Caballero ◽  
Donella Beckwith ◽  
Nadezhda V. Shilova ◽  
Adele Gabba ◽  
Tanja J. Kutzner ◽  
...  
Keyword(s):  
Protein Design ◽  
Full Range ◽  
Biological Information ◽  
The Novel ◽  
Modular Assembly ◽  
Binding Partners ◽  
Galectin 3 ◽  
Potential Applications ◽  
Similar Affinity

Abstract The concept of biomedical significance of the functional pairing between tissue lectins and their glycoconjugate counterreceptors has reached the mainstream of research on the flow of biological information. A major challenge now is to identify the principles of structure–activity relationships that underlie specificity of recognition and the ensuing post-binding processes. Toward this end, we focus on a distinct feature on the side of the lectin, i.e. its architecture to present the carbohydrate recognition domain (CRD). Working with a multifunctional human lectin, i.e. galectin-3, as model, its CRD is used in protein engineering to build variants with different modular assembly. Hereby, it becomes possible to compare activity features of the natural design, i.e. CRD attached to an N-terminal tail, with those of homo- and heterodimers and the tail-free protein. Thermodynamics of binding disaccharides proved full activity of all proteins at very similar affinity. The following glycan array testing revealed maintained preferential contact formation with N-acetyllactosamine oligomers and histo-blood group ABH epitopes irrespective of variant design. The study of carbohydrate-inhibitable binding of the test panel disclosed up to qualitative cell-type-dependent differences in sections of fixed murine epididymis and especially jejunum. By probing topological aspects of binding, the susceptibility to inhibition by a tetravalent glycocluster was markedly different for the wild-type vs the homodimeric variant proteins. The results teach the salient lesson that protein design matters: the type of CRD presentation can have a profound bearing on whether basically suited oligosaccharides, which for example tested positively in an array, will become binding partners in situ. When lectin-glycoconjugate aggregates (lattices) are formed, their structural organization will depend on this parameter. Further testing (ga)lectin variants will thus be instrumental (i) to define the full range of impact of altering protein assembly and (ii) to explain why certain types of design have been favored during the course of evolution, besides opening biomedical perspectives for potential applications of the novel galectin forms.

scholarly journals Molecular Characterization of the Gene Encoding a New AmpC β-Lactamase in a Clinical Strain of Acinetobacter Genomic Species 3

2004 ◽  
Vol 48 (4) ◽  
pp. 1374-1378 ◽  
Author(s):  
Alejandro Beceiro ◽  
Lourdes Dominguez ◽  
Anna Ribera ◽  
Jordi Vila ◽  
Francisca Molina ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Characterization ◽  
Biochemical Properties ◽  
Clinical Strain ◽  
The Novel ◽  
Gene Encoding ◽  
Genomic Species ◽  
First Time

ABSTRACT A presumptive chromosomal cephalosporinase (pI, 9.0) from a clinical strain of Acinetobacter genomic species 3 (AG3) is reported. The nucleotide sequence of this β-lactamase shows for the first time the gene encoding an AmpC enzyme in AG3. In addition, the biochemical properties of the novel AG3 AmpC β-lactamase are reported

Energy Saving Preparation and Evaluation of a Novel Polycarboxylate Superplasticizer

2021 ◽  
Vol 1035 ◽  
pp. 1006-1012
Author(s):  
Xiao Liu ◽  
Xiao Fei Song ◽  
Qi Feng Luo ◽  
Chun Lei Xia ◽  
Yun Sheng Zheng ◽  
...  
Keyword(s):  
Energy Saving ◽  
Building Materials ◽  
Equation Model ◽  
Order Kinetic ◽  
The Novel ◽  
Cement Pastes ◽  
Polycarboxylate Superplasticizer ◽  
Low Emission ◽  
Potential Applications ◽  
Preparation And Evaluation

A novel polycarboxylate superplasticizer (PCE) with energy saving preparation was elaborately designed and synthesized by using acrylic acid (AA), hydroxypropyl acrylate (HPA) and isopentenyl polyethylene glycol (IPEG) as monomers. To investigate the effects of the preparation method on the effectiveness of PCE, the PCEs were prepared from energy-saving method and common method respectively, and the hydration heat evolutions of the cement pastes containing these PCEs were comparatively probed. Furthermore, the working mechanisms of the PCEs by different preparations were identified via adsorption behavior, adsorption kinetic and Zeta potential of the PCE on cement surfaces. The results showed that, this novel PCE prepared in an energy saving manner can significantly prolong the hydration process and present a stronger adsorption capacity. In addition, the adsorption of this PCE on cement surface exhibited a characteristic of pseudo first order kinetic equation model. The evaluation in energy conservation showed that, this energy saving preparation can save 1.548×104 kJ per 10 ton production. The aim of this study is to provide a new avenue to synthesize a PCE with economical method which achieves energy-saving preparation. Due to the indispensable application in construction industry, the innovations from this study contribute to the low energy-consumption production and high eco-effectiveness of the novel PCE, which has potential applications in low-emission building materials.

Fish gelatin: The novel potential applications

2019 ◽  
Vol 63 ◽  
pp. 103581 ◽  
Author(s):  
Lin-Chen Lv ◽  
Qing-Yun Huang ◽  
Wen Ding ◽  
Xing-Hua Xiao ◽  
Hong-Yan Zhang ◽  
...  
Keyword(s):  
The Novel ◽  
Fish Gelatin ◽  
Potential Applications

scholarly journals Biochemical Properties and Potential Applications of Recombinant Leucine Aminopeptidase from Bacillus kaustophilus CCRC 11223

2011 ◽  
Vol 12 (11) ◽  
pp. 7609-7625 ◽  
Author(s):  
Yanfei Shen ◽  
Fanghua Wang ◽  
Dongming Lan ◽  
Yuanyuan Liu ◽  
Bo Yang ◽  
...  
Keyword(s):  
Leucine Aminopeptidase ◽  
Biochemical Properties ◽  
Bacillus Kaustophilus ◽  
Potential Applications

Prediction of disinfection by-product formation in drinking water via fluorescence spectroscopy

2016 ◽  
Vol 2 (2) ◽  
pp. 383-389 ◽  
Author(s):  
Benjamin F. Trueman ◽  
Sean A. MacIsaac ◽  
Amina K. Stoddart ◽  
Graham A. Gagnon
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Fluorescence Spectroscopy ◽  
Statistical Learning ◽  
Learning Algorithms ◽  
Product Formation ◽  
The Novel ◽  
Potential Applications ◽  
By Products

Fluorescence spectroscopy has potential applications for monitoring disinfection by-products (DBPs) during water treatment. This paper demonstrates the novel application of several statistical learning algorithms for fluorescence-based DBP prediction.

scholarly journals SentiMental: An emotional profiling algorithm for identifying affect patterns in text

2018 ◽  
Author(s):  
Massimo Stella
Keyword(s):  
Natural Language Processing ◽  
Natural Language ◽  
Language Processing ◽  
Processing Algorithm ◽  
Copyright Holder ◽  
The Novel ◽  
Novel Approach ◽  
Technical Report ◽  
Potential Applications ◽  
Natural Language Processing Algorithm

This technical report outlines the mechanisms and potential applications of SentiMental, a suite of natural language processing algorithm designed and implemented by Massimo Stella, Complex Science Consulting. The following technical report briefly outlines the novel approach of SentiMental in performing sentiment and emotional analysis by directly harnessing the whole structure of the mental lexicon rather than by using affect norms. Furthermore, this technical report outlines the direct emotional profiling and the visualisations currently implemented in version 0.1 of SentiMental. Features under development and current limitations are also outlined and discussed.This technical report is not meant as a publication. The author holds full copyright and any reproduction of parts of this report must be authorised by the copyright holder. SentiMental represents a work in progress, so do not hesitate to get in touch with the author for any potential feedback.

scholarly journals Polaromonas hydrogenivorans sp. nov., a psychrotolerant hydrogen-oxidizing bacterium from Alaskan soil

2007 ◽  
Vol 57 (3) ◽  
pp. 616-619 ◽  
Author(s):  
Maria Sizova ◽  
Nicolai Panikov
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Biochemical Properties ◽  
Rrna Gene ◽  
Organic Substrates ◽  
The Novel ◽  
Membrane Phospholipids ◽  
Wide Range ◽  
Physiological And Biochemical ◽  
Physiological And Biochemical Properties

Psychrotolerant (0–25 °C), chemolithotrophic Gram-negative cocci were isolated from Alaskan forest soil. The novel isolate was found to grow autotrophically on H2 : CO2 mixtures and to switch to heterotrophic growth on media containing organic substrates. The novel strain utilized a wide range of organic acids, some simple sugars and alcohols. Naphthalene vapour did not support growth. On the basis of 16S rRNA gene sequence similarity, the novel strain is affiliated to the genus Polaromonas, of the class Betaproteobacteria, and is related to Polaromonas naphthalenivorans (99.6 % gene sequence similarity), Polaromonas aquatica (97.4 %) and Polaromonas vacuolata (96.1 %). The membrane phospholipids contained 16 : 1ω7c/16 : 1ω6c, 16 : 0 and 18 : 1ω7c, similar to the fatty acids found for P. naphthalenivorans, P. aquatica and P. vacuolata. On the basis of DNA–DNA hybridization, physiological and biochemical properties, the hydrogen-oxidizing mixotrophic isolate represents a novel species, for which the name Polaromonas hydrogenivorans sp. nov. is proposed. The type strain is DSM 17735T (=NRRL B-41369T).

scholarly journals Immunopathogenesis of COVID-19 and early immunomodulators

2020 ◽  
Vol 63 (7) ◽  
pp. 239-250 ◽  
Author(s):  
Kyung-Yil Lee ◽  
Jung-Woo Rhim ◽  
Jin-Han Kang
Keyword(s):  
Lung Injury ◽  
Early Stage ◽  
Severe Pneumonia ◽  
Biochemical Properties ◽  
Etiologic Agent ◽  
Host Immune System ◽  
The Novel ◽  
Immune Mediated ◽  
Novel Coronavirus ◽  
Current Guidelines

The novel coronavirus disease 2019 (COVID-19) is spreading globally. Although its etiologic agent is discovered as severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), there are many unsolved issues in COVID-19 and other infectious diseases. The causes of different clinical phenotypes and incubation periods among individuals, species specificity, and cytokine storm with lymphopenia as well as the mechanism of damage to organ cells are unknown. It has been suggested that in viral pneumonia, virus itself is not a direct cause of acute lung injury; rather, aberrant immune reactions of the host to the insults from viral infection are responsible. According to its epidemiological and clinical characteristics, SARS-CoV-2 may be a virus with low virulence in nature that has adapted to the human species. Current immunological concepts have limited ability to explain such unsolved issues, and a presumed immunopathogenesis of COVID-19 is presented under the proteinhomeostasis-system hypothesis. Every disease, including COVID-19, has etiological substances controlled by the host immune system according to size and biochemical properties. Patients with severe pneumonia caused by SARS-CoV-2 show more severe hypercytokinemia with corresponding lymphocytopenia than patients with mild pneumonia; thus, early immunomodulator treatment, including corticosteroids, has been considered. However, current guidelines recommend their use only for patients with advanced pneumonia or acute respiratory distress syndrome. Since the immunopathogenesis of pneumonia may be the same for all patients regardless of age or severity and the critical immune-mediated lung injury may begin in the early stage of the disease, early immunomodulator treatment, including corticosteroids and intravenous immunoglobulin, can help reduce morbidity and possibly mortality rates of older patients with underlying conditions.

Synthesis, physico-chemical and biochemical properties of the novel tri-, tetra- and pentaphosphate mRNA cap analogues

2002 ◽  
Author(s):  
Jacek Jemielity ◽  
Joanna Zuberek ◽  
Janusz Stępiński ◽  
Magdalena Lewdorowicz ◽  
Anna Niedzwiecka ◽  
...  
Keyword(s):  
Biochemical Properties ◽  
The Novel ◽  
Physico Chemical
Sign in / Sign up

Export Citation Format

Share Document