Effects of Electroacupuncture on Bone Mineral Density, Oestradiol Level and Osteoprotegerin Ligand Expression in Ovariectomised Rabbits

Objectives To investigate the effects of electroacupuncture (EA) on the oestradiol level, bone mineral density and osteoprotegerin ligand (OPGL) expression, and to explore whether EA might be a complementary method to prevent and treat osteoporosis. Methods A total of 21 New Zealand rabbits were randomly divided into three groups: a normal control (NC) group undergoing no surgery or EA; an ovariectomised (OVX) group, in which rabbits were ovariectomised but did not receive EA; an EA group, in which rabbits were ovariectomised and treated with EA. Acupuncture was applied at ST35, BL20 and BL23 points bilaterally. EA (10 Hz, 2 mA) was applied bilaterally at BL20 and BL23 for 30 min a day for 14 days. After 14 days, all animals were killed. OPGL expression level was determined by immunohistochemistry. Blood serum levels of oestradiol were measured by ELISA and bone mineral density was detected by dual-energy x-ray absorptiometry. Results After ovariectomy, the bone mineral density and oestradiol level decreased significantly in the OVX group compared with the NC group (p=0.001), whereas the OPGL expression level increased. After EA, the bone mineral density and oestradiol level increased compared with the OVX group (p=0.049 and p=0.012, respectively). The OPGL level OPGL level in the EA group was lower than that in the OVX group (p=0.022). Conclusions EA restored bone mineral density towards normal and was associated with increased plasma oestradiol level and reduced OPGL expression in an ovariectomised rabbit model of osteoporosis.

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P013 Serum osteoprotegerin and RANKL in patients with Juvenile Idiopathic Arthritis and their correlation with bone mineral density

Rheumatology ◽

10.1093/rheumatology/keab722.005 ◽

2021 ◽

Vol 60 (Supplement_5) ◽

Author(s):

Radwa Helmy Shalaby ◽

Elham Mohamed Kassem ◽

Nagat Mohamed El-Gazzar ◽

Sahar Ahmed Fathy Hammoudah ◽

Amal Mohamed El-Barbary

Keyword(s):

Bone Mineral Density ◽

Juvenile Idiopathic Arthritis ◽

Lumbar Spine ◽

Bone Mineral ◽

Disease Duration ◽

Serum Levels ◽

Z Score ◽

Low Bone Mass ◽

Mineral Density ◽

Serum Rankl

Abstract Background Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic arthropathy of childhood and is associated with low bone mass, and may hasten the onset of osteoporosis later in life1. Bone loss occurs because of an imbalance between osteoclasts-activating factors like receptor activator of nuclear factor-κB ligand (RANKL) and its inhibitor osteoprotegerin (OPG) 2. Dual energy X-ray absorptiometry (DXA) is the preferred method for measuring bone mineral density (BMD) in children and to identify and follow individuals at risk for fracture 3. The objective is the Evaluation of serum levels of osteoprotegerin and RANKL and their correlation with BMD in JIA patients. Methods Forty JIA patients (according to the revised classification criteria of ILAR) and 40 healthy children individually matched for age, sex and race were included in this study. Children excluded from the study were those with primary and secondary causes of osteoporosis (such as chronic illness). All patients were assessed clinically by: age, sex, body mass index, type of JIA, disease duration and disease activity (by Juvenile Arthritis Disease Activity Score; JADAS 10). The functional disability was assessed by the Childhood Health Assessment Questionnaire (CHAQ). Blood samples were collected from JIA patients and healthy controls to determine serum levels of OPG and RANKL by ELISA. DXA scans were done using GE Healthcare Lunar DPX, Madison, Wisconsin. Bone mineral density of the L1-L4 lumbar spine and total body less head (TBLH) was evaluated in g/cm2 and expressed as Z score for age, sex according to the reference data given for this equipment. Results The study included 40 patients (25 females) with a mean age of 11.14 years and median disease duration of 2.5 years. As regard JIA type, 45% of patients were oligoarticular, 32.5% were polyarticular, and 22.5% were systemic JIA. Median JADAS 10 was 13.95. Patients (especially polyarticular JIA) had significantly higher serum RANKL levels and lower serum OPG and OPG/RANKL ratio when compared with controls (with p-value <0.001, 0.032 and <0.001 respectively). A diagnosis of low BMD (BMD Z-score ≤ -2) was given in 25% of patients (15% polyarticular and 10% systemic) by DXA of lumbar spine, and 20% (10% polyarticular and 10% systemic) by DXA of TBLH. On the other hand, no patient was given a diagnosis of osteoporosis (BMD Z-score ≤ -2 and a significant fracture history). Low BMD at lumbar spine and TBLH was negatively correlated with serum RANKL while positively correlated with OPG/RANKL ratio. Moreover, low BMD at lumbar spine was positively correlated with serum OPG level Conclusion High RANKL and low OPG levels appear to be associated with low bone mass in JIA patients. Patients with JIA (especially polyarticular and systemic subtype) are at increased risk of low bone mineral mass. Disclosure of Interests None declared

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Association of dietary consumption and serum levels of vitamin A and β-carotene with bone mineral density in Chinese adults

Bone ◽

10.1016/j.bone.2015.05.028 ◽

2015 ◽

Vol 79 ◽

pp. 110-115 ◽

Cited By ~ 14

Author(s):

Geng-dong Chen ◽

Ying-Ying Zhu ◽

Yi Cao ◽

Jun Liu ◽

Wen-qi Shi ◽

...

Keyword(s):

Bone Mineral Density ◽

Vitamin A ◽

Bone Mineral ◽

Serum Levels ◽

Mineral Density ◽

Chinese Adults ◽

Dietary Consumption ◽

Β Carotene

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Increased serum levels of the Wnt antagonist Dicckopf-1 (DKK1) and impaired trabecular bone mineral density using QCT scan in acromegalic patients

Endocrine Abstracts ◽

10.1530/endoabs.37.ep798 ◽

2015 ◽

Author(s):

Elena Valassi ◽

Iris Crespo ◽

Anna Aulinas ◽

Eulalia Urgell ◽

Jorge Malouf ◽

...

Keyword(s):

Bone Mineral Density ◽

Trabecular Bone ◽

Bone Mineral ◽

Serum Levels ◽

Mineral Density ◽

Trabecular Bone Mineral Density

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Bone Mineral Density, Parathyroid Hormone, and Vitamin D After Gastric Bypass Surgery: a 10-Year Longitudinal Follow-Up

Obesity Surgery ◽

10.1007/s11695-020-04912-7 ◽

2020 ◽

Vol 30 (12) ◽

pp. 4995-5000

Author(s):

Mustafa Raoof ◽

Ingmar Näslund ◽

Eva Rask ◽

Eva Szabo

Keyword(s):

Bone Mineral Density ◽

Vitamin D ◽

Gastric Bypass ◽

Parathyroid Hormone ◽

Femoral Neck ◽

Bone Mineral ◽

Gastric Bypass Surgery ◽

Serum Levels ◽

Massive Weight Loss ◽

Mineral Density

Abstract Background The aim of the present study was to study longitudinal changes in bone mineral density (BMD), vitamin D, and parathyroid hormone (PTH) levels in females over a 10-year period after laparoscopic Roux-en-Y gastric bypass (LRYGB). Methods Twenty-three women, mean age 43.4 ± 8.7 years and mean body mass index (BMI) 44.6 ± 5.17 kg/m2 at baseline, were included. BMD, BMI, S-calcium, S-25(OH)-vitamin D, and fP-PTH were measured preoperatively and 2, 5, and 10 years postoperatively. Results Ten years after surgery, BMD of the spine and femoral neck decreased by 20% and 25%, respectively. Changes in serum levels of vitamin D, PTH, and calcium over the same period were small. Conclusion After LRYGB with subsequent massive weight loss, a large decrease in BMD of the spine and femoral neck was seen over a 10-year postoperative period. The fall in BMD largely occurred over the first 5 years after surgery.

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