scholarly journals AB0596 THE POSSIBLE CONTRIBUTION OF DEHYDROEPIANDROSTERONE SULFATE FOR OSTEOARTHRITIS OF THE KNEE

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1335.1-1335
Author(s):  
I. Bashkova ◽  
I. Madyanov ◽  
K. Misko ◽  
E. Preobrazhenskaia
Keyword(s):  
Factor Analysis ◽  
Risk Factor ◽  
Experimental Studies ◽  
Dehydroepiandrosterone Sulfate ◽  
Knee Joints ◽  
Control Group ◽  
Inflammatory Factor ◽  
X Ray ◽  
Age Related ◽  
Anti Inflammatory

Background:Genetic, biochemical, metabolic, hormonal (primarily imbalance of sex hormones) factors are involved in the progression of osteoarthritis (OA). Dehydroepiandrosterone and its metabolite, dehydroepiandrosterone sulfate (DHEA-S), have a stress-limiting, anti-atherogenic, anti-diabetic, antihypertensive, anti-infective, immunomodulatory effects. Heroprotective effect is not excluded. Experimental studies have identified a relationship between an age-related decrease in DHEA-S levels and various adverse effects of aging.Objectives:To identify the contribution of DHEA-S to the pathogenesis of OA, it`s advisable to conduct a comparative analysis of connection of the adrenal hormones with clinical, laboratory, radiological signs of the course of OA.Methods:Patients with primary OA with a lesion of the knee joints (n=90, including 22 men) were examined. The age of the patients - 29-69 years, the duration of the disease – 1.5-20 years. The control group (n=114, including 26 men) was formed by random sampling of the population from healthy people, it`s representative by gender and age. We investigated the serum levels of cortisol, DHEA-S, estradiol (in women), testosterone (in men) and carried out radiography of the knee joints. OA was diagnosed using R.D. Althman, the x-ray stage-according to the classification of Kellgren and Lawrence. Statistically determined the mean value, standard deviation. Differences between the samples were considered statistically significant at p<0.05. To create a model of OA pathogenesis, the method of principal components of factor analysis was used.Results:The DHEA-S level in the blood of patients with OA was lower than that of the control group (2.40±1.20 vs 3.66±1.45 μg/ml, p=0.001),in women-was lower, than in men (2.25±1.17 vs 2.89±1.23 μg/ml, p=0.045). In the control group, gender differences were not statistically significant (p>0.05). All patients with OA showed an inverse correlation between age and DHEA-S (r=-0.511, p=0.0001, and r=-0.549, p=0.0001 respectively). For factor analysis the most important signs for the course of OA are ESR levels, C-reactive protein (CRP) in the blood (as markers of inflammatory component of OA, or factor 2) were selected, and the radiological stage of OA (degenerative component, or factor 1). In women, we regarded factor 1 as «degenerative», the maximum contribution to total dispersion was made by the «x-ray stage» (+0.72). This symptom was opposed by the «DHEA-S level» (-0.79) and «estradiol blood level» (-0.68), which suggests a link between degenerative and dystrophic processes in the knee joint in women with OA and a decrease in blood levels of DHEA-S and estradiol. Factor 2 we interpreted as «inflammatory». This was indicated by the values of «CRP» (+0.66) and «ESR» (+0.64). The «inflammatory» factor in women from hormonal indicators was opposed by the «blood cortisol content» (-0.31) and «DHEA-S level» (-0.26). Factor 1 in men accounted for 46% of the total variance. Since factor 1 in men included the most significant «CRP» (+0.85), «X-ray stage» (+0.77) and «ESR» (+0.72), we called it «antidegenerative anti-inflammatory factor». The maximum value (modulo) in factor 1 is for DHEA-S (-0.76) and the lower is for testosterone (-0.53). So, in men, a sufficient level of DHEA-S is closely related to the «antidegenerative-anti-inflammatory» factor of OA pathogenesis and DHEA-S counteracts 2 key pathogenetic processes simultaneously-degenerative and inflammatory.Conclusion:In women, a decrease in DHEA-S is a risk factor for the predominantly degenerative component of OA, in men it`s a universal risk factor, predisposing both to the development of inflammation and degenerative changes in the jointsDisclosure of Interests:None declared.

scholarly journals Nekotorye aspekty problemy narusheniyaosanki u podrostkov Primorskogo kraya

2007 ◽  
Vol 10 (2) ◽  
pp. 2-4
Author(s):  
T G VASIL'EVA ◽  
E A KOChETKOVA
Keyword(s):  
Risk Factor ◽  
Bone Metabolism ◽  
Dual Energy ◽  
Control Group ◽  
Phosphorus Concentration ◽  
Blood Calcium ◽  
X Ray ◽  
Different Types ◽  
Calcium Exchange ◽  
Mineral Bone Density

Objective of study was to examine mineral bone density and condition of bone metabolism in adolescent with different types of carriage disorders. Material and methods: We investigated 122 adolescent with carriage disorders, its dates of dual - energy X - ray absorbtiometry. Condition of bone metabolism was estimated on blood osteocalcini and ßCrossLaps. Condition of calcii-phosphorum exchange was estimated on blood calcium (Ca) and phosphorus concentration and also its level in urinary daily. PTH secretion in the serum blood was estimated. Control group was consisted by 70 health adolescent similar ages and sex. Results: 48% adolescences with carriage disorders had osteopenic syndrome and dysfunction of calcii-phosphorum exchange and bone metabolism in compare control group. Conclusion: carriage disorders and long conservation of negative calcium exchange lead to bone metabolism dysfunction and is one's risk factor development of osteopenic syndrome.

scholarly journals Allium sativum L. (Garlic) Role in Osteoarthritis: A Systematic Review of Clinical Trials

2021 ◽  
Vol 11 (4) ◽  
pp. 12104-12119
Keyword(s):  
Systematic Review ◽  
Clinical Trials ◽  
Side Effects ◽  
Allium Sativum ◽  
Inflammatory Diseases ◽  
Control Group ◽  
Therapeutic Effects ◽  
Inflammatory Factor ◽  
Anti Inflammatory ◽  
Over Time

Osteoarthritis is a chronic degenerative disease involving the joints and bones, causing their degradation over time. Inflammation, pain, and stiffness in joints are indicators of the disease. Pharmacotherapy cannot always be efficient and may cause side effects. So, adjuncts such as complementary herbs have become of note. Garlic is a herb well-known for its various therapeutic effects such as anti-bacterial, anti-hypertension, antioxidant and anti-inflammatory effects. Due to garlic's widespread use, studying its effects and mechanisms on inflammatory diseases such as osteoarthritis has been noteworthy. We searched Science Direct, Pubmed, Cochrane, and Google Scholar databases for all articles published until October 2020, based on PRISMA. Searched keywords were the following: [(garlic and arthritis), (garlic and osteoarthritis), (Garlic and OA), (Allium sativum and arthritis), (Allium sativum and osteoarthritis), (Allium sativum and OA)]. The results showed garlic, and its constituents have remarkable effects on improving OA symptoms through antioxidant and anti-inflammatory pathways. Our review shows that groups receiving garlic as a treatment showed a significant reduction in pain and inflammatory factor levels and an improved physical function instead of the control group.

Renal aging resembles a continuum between normal and diseased kidneys that potentiates inflammatory response to injury

2020 ◽  
Author(s):  
Su Woong Jung ◽  
Dong Jin Kim ◽  
Yang Gyun Kim ◽  
Ju-Young Moon ◽  
Kyung Hwan Jeong ◽  
...  
Keyword(s):  
Immune System ◽  
Inflammatory Response ◽  
T Cell Activation ◽  
Experimental Studies ◽  
Kidney Damage ◽  
Control Group ◽  
Immune System Activation ◽  
Age Related ◽  
Renal Aging ◽  
Young Mice

Abstract Renal aging is a natural process that can lead to structural changes and functional decline in the kidneys. These age-related changes are considered irreversible physiological processes, but resemble diseased kidneys. To enhance understanding of the molecular nature of renal aging, we first compared whole-kidney RNA sequencing between naturally aging mice (24-month-old) and adenine-induced nephropathy in young mice (2-month-old). Young mice (2-month-old) without intervention were used as the control group to investigate transcription alteration with aging or by adenine-enriched diet. Next, we compared the functional and structural renal consequences of aging and adenine-induced nephropathy between young (2-month-old) and old mice (18- to 22-month-old). C57BL/6 male mice were used in all experimental studies. Both aging kidneys and adenine-induced nephropathy showed similar transcriptional profiles characterized by upregulation in innate and adaptive immune system activation and inflammation, although these alterations were generally less significant in the aging kidneys. In contrast to aging kidneys, adenine-induced nephropathy showed prominent expression of the genes related to cytokines, T cell activation, and fibrosis and decreased expression of the genes implicated in transporter activity and metabolism. The subclinical immunological micromilieu in aging kidneys potentially causes augmented kidney damage in response to injurious stimulus. When mice were fed with adenine-enriched diet, aging kidneys showed more extensive tubular injury and fibrosis with stronger inflammatory response than young kidneys. Taken together, our results suggest that renal aging may lie on a continuum between normal kidneys and diseased kidneys in the context of immune system upregulation that can worsen kidney damage upon injury.

scholarly journals MULTIMORBIDITY AS ONE OF RISK PREDICTORS FOR CONTRAST INDUCED NEPHROPATHY IN GERIATRIC PRACTICE

2017 ◽  
Vol 21 (1) ◽  
pp. 34-38 ◽  
Author(s):  
A. I. Ariev ◽  
A. A. Chesnokov ◽  
S. D. Dzakhova ◽  
N. A. Ovsiannikova ◽  
G. T. Arieva
Keyword(s):  
Risk Factor ◽  
Age Groups ◽  
Endovascular Intervention ◽  
Control Group ◽  
Contrast Induced Nephropathy ◽  
Creatinine Concentration ◽  
X Ray ◽  
Endovascular Interventions ◽  
Before And After ◽  
Risk Predictors

AIM: research of multimorbidity as a risk factor for contrast induced nephropathy after X-ray endovascular intervention in patients of senior age groups. PATIENTS AND METHODS. Patients of following age groups were examined: 34 – 59 years (49,7±7,8 years) – 35 people (control group), 60 – 74 years (66,1±4,1) – 48 people and 75 – 82 years (78,8±4,2) – 22 people. Kidneys functional status examination was performed in patients of advanced and senile age before and after 24, 48 120 hours after X-ray endovascular intervention. Effect of multimorbidity states on contrast induced nephropathy (CIN) development. RESULTS. Strong correlation between different multimorbidity factors, serum creatinine concentration in different time periods after X-ray endovascular intervention and risk of CIN was revealed. CONCLUSION: in patients of advanced and senile age multimorbidity is a risk factor for CIN development when carrying out X-ray endovascular interventions. 

Abstract TP279: Brain-Derived Microparticles Mediate Neuroinflammation After Stroke

Stroke ◽  
2020 ◽  
Vol 51 (Suppl_1) ◽  
Author(s):  
Zhili Chen ◽  
Michael Chopp ◽  
Alex Zacharek ◽  
Wei Li ◽  
Poornima Venkat ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Neurological Deficits ◽  
Cell Infiltration ◽  
Control Group ◽  
Lesion Volume ◽  
Inflammatory Factor ◽  
Ischemic Brain ◽  
Factor Expression ◽  
Anti Inflammatory

Background and Purpose: Microparticles (MPs, ~ size between 0.1-1mm) are lipid encased containers, and are involved in intercellular communication and regulate inflammation. Stroke increases brain derived MP (BDMP) secretion which induces neuroinflammation. Milk fat globule-EGF factor-8 (MFGE8) promotes apoptotic cell clearance and limits pathogenic antigen cross presentation. In this study, we investigate whether BDMP affects stroke-induced neuroinflammation; whether MFGE8 treatment reduces stroke or BDMP-induced neuroinflammation and improves functional outcome after stroke. Method: 1) BDMPs were extracted from ischemic brain 24h after dMCAo by ultracentrifugation. 2) Adult (8 months) male C57BL/6J mice were subjected to dMCAo and were injected via tail vein 3h after stroke with: A) PBS (n=5/group); B) +BDMP(1.5х10 8 ,n=6/group); C)+MFGE8 (Lactadherin, 400ug/kg, n=5/group); D) +BDMP+MFGE8 (n=6/group). A battery of neurological function outcomes and immunostaining were performed. Blood plasma was used for Western blot assay. Result: 1) Compared with the Stroke+PBS control group, Stroke+BDMP significantly increases inflammatory factor expression in the circulation, increases lesion volume, neurological deficits, blood brain barrier (BBB) leakage, microglial activation, and inflammatory cell infiltration (CD45, microglia/macrophage, Neutrophils) and inflammatory factor (TNFα, IL6, IL1β) expression in brain, and increases axon/white matter (WM) damage; 2) Compared to Stroke+PBS and Stroke+BDMP groups, Stroke+MFGE8 and Stroke+BDMP+MFGE8 mice exhibited significantly improved neurological outcome; decreased lesion volume and BBB leakage, reduced axon/WM damage, and decreased inflammatory cell infiltration and inflammatory factor expression in the ischemic border, respectively. MFGE8 treatment significantly increased anti-inflammatory factor (IL10) expression in ischemic brain, and decreased IL1β expression in circulation compared to Stroke+PBS and Stroke+BDMP groups, respectively. Conclusion: BDMP increases neuroinflammation and induces worse brain damage after stroke. MFGE8 treatment reduces stroke and BDMP-induced neurological deficits possibly via its anti-inflammatory effects.

Dehydroepiandrosterone Sulfate (DHEAS) Secretion in Early and Advanced Solid Neoplasms: Selective Deficiency in Metastatic Disease

1998 ◽  
Vol 13 (3) ◽  
pp. 154-157 ◽  
Author(s):  
P. Lissoni ◽  
F. Rovelli ◽  
L. Giani ◽  
M. Mandala ◽  
S. Meregalli ◽  
...  
Keyword(s):  
Advanced Cancer ◽  
Experimental Studies ◽  
Biological Significance ◽  
Serum Levels ◽  
Dehydroepiandrosterone Sulfate ◽  
Control Group ◽  
Neoplastic Progression ◽  
Antitumor Effects ◽  
Significant Difference ◽  
Preliminary Study

Several endogenous hormones have been proven to stimulate cancer growth, whereas at present very few hormones are known to display oncostatic activity. The most widely investigated antitumor hormone is the pineal indole melatonin (MLT), and cancer progression has been shown to be associated with a decline in MLT secretion. Recently, another hormone, the adrenal steroid dehydroepiandrosterone-sulfate (DHEAS), has appeared to exert antitumor effects similar to those previously described for MLT In addition, experimental studies suggest a diminished DHEAS production with neoplastic progression. This preliminary study was performed to evaluate the daily secretion of DHEAS in a group of early and advanced cancer patients. The study included 70 patients with solid tumors (gastrointestinal tract tumors: 28; breast cancer: 24; non-small cell lung cancer: 18), 28 without and 42 with distant metastases. The serum levels of DHEAS were measured by RIA in blood samples collected in the morning. The control group consisted of 100 age- and sex-matched healthy subjects. No significant difference in mean serum levels of DHEAS was observed between controls and non-metastatic patients. In contrast, metastatic patients, irrespectively of tumor histotype, showed significantly lower mean levels of DHEAS with respect to either controls or non-metastatic patients. Moreover, metastatic patients with visceral locations showed significantly lower values of DHEAS than those with bone or soft-tissue metastases. This preliminary study would suggest there to be a deficiency in the daily DHEA secretion in patients with disseminated cancer. Further studies evaluating circadian DHEAS secretion in relation to that of the pineal hormone MLT will be required to better define the biological significance of the advanced cancer-related decline in endogenous DHEAS production.

scholarly journals The Effect of Trehalose in Inhibiting Liver Damage via The Il-6 Pathway in Old Mice

2021 ◽  
Vol 2 (2) ◽  
pp. 9-19
Author(s):  
Rabiatul Aminah ◽  
Arif Santoso ◽  
Muh Husni Cangara ◽  
Marhaen Hardjo ◽  
Aminuddin Aminuddin ◽  
...  
Keyword(s):  
Liver Damage ◽  
Vascular Inflammation ◽  
Inflammatory Factors ◽  
Control Group ◽  
Inflammatory Factor ◽  
Control Group Design ◽  
Age Related ◽  
Post Test ◽  
Effective Therapeutic Strategy ◽  
Old Rats

Trehalose is a non-reducing disaccharide consisting of two glucose molecules linked by a 1–1 glycosidic bond. Trehalose can play a role in organ detoxification, antioxidant enzyme activity, reducing lipid peroxidation and reducing the secretion of inflammatory factors TNF-, IL-1β, Il-6, thereby inhibiting liver damage. Because of its role, this study aims to determine the effect of trehalose administration on liver damage through the IL-6 expression pathway with experimental post-test design with control group design. This research was conducted using samples of old rats, namely Wistar rats (Rattus novergicus). The old rats used were 21 male species which were then divided into 3 groups, namely the old control group, the old rat group that was given sucrose, and the old rat group that was given trehalose. Then observed for 8 weeks, namely in March - May 2021 at the Faculty of Medicine, Hasanuddin University Makassar. The results obtained from the 3 groups showed a significant value that the administration of trehalose sugar in elderly mice had an effect on reducing the inflammatory factor IL-6 because it activated autophagy in macrophages thereby reducing cytokine production and vascular inflammation so as to be able to restore all aging mice to a level like observed in young mice. It is also known that aged rat liver, which is characterized by ER UPR activation and inflammatory signaling, decreases nicotinamide and UDP-N-acetyl-hexosamines, and reduces proteasome activity. Therefore, trehalose can be an effective therapeutic strategy against age-related disorders of proteostasis in the liver.

scholarly journals Anti-Inflammatory Properties In Vitro and Hypoglycaemic Effects of Phenolics from Cultivated Fruit Body of Phellinus baumii in Type 2 Diabetic Mice

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2285
Author(s):  
Kai Yang ◽  
Su Zhang ◽  
Yan Geng ◽  
Baoming Tian ◽  
Ming Cai ◽  
...  
Keyword(s):  
Dietary Intervention ◽  
Fruit Body ◽  
Raw 264.7 Cells ◽  
Control Group ◽  
Inflammatory Factor ◽  
Dependent Manner ◽  
Diabetic Mice ◽  
Phellinus Baumii ◽  
Anti Inflammatory

Dietary intervention in type 2 diabetes mellitus (T2DM) is a hotspot in international research because of potential threats to human health. Phellinus baumii, a wild fungus traditionally used as a food and medicine source, is now cultivated in certain East Asian countries, and is rich in polyphenols, which are effective anti-inflammatory ingredients useful in treatment of T2DM, with fewer side effects than drugs. To examine the hypoglycaemic effects of Phellinus baumii phenolics (PPE), the metabolite profiles of T2DM mice induced by streptozotocin after PPE intervention were systematically analyzed. Here, 10 normal mice were given normal saline as control group, and 50 model mice were randomly assigned to five groups and daily intragastric administrated with saline, metformin (100 mg/kg), and PPE (50, 100, 150 mg/kg of body weight), for 60 days. The pro-inflammatory factor contents of lipopolysaccharide stimulation of RAW 264.7 cells were decreased in a dose-dependent manner after PPE treatment, we propose that PPE could exert anti-inflammatory properties. PPE could also effectively reduce blood glucose levels, increased insulin sensitivity, and improved other glucolipid metabolism. Q-PCR results suggested that the hypoglycemic effects of PPE might be through activating IRS1/PI3K/AKT pathway in diabetic mice. These results suggest that PPE has strong potential as dietary components in the prevention or management of T2DM.

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