Identification of human papillomavirus in oral rinse specimens from women with and without cervical intraepithelial lesions

ObjectivesThe objective of this study was to evaluate the frequency of human papillomavirus (HPV) in the oral cavity of women with and without abnormal cervical cytology and to determine whether there is an association of oral HPV infection with infection of the cervix or with cervical cancer precursor lesions.MethodsThe present study was conducted among 406 women, aged 18–82 years, who attended the Prevention Department of Barretos Cancer Hospital (HCB), Brazil due to a previous altered cervical cytology result. Oral rinse, cervical cytology and biopsy were collected at the same day. The participants also answered a questionnaire about socioeconomic characteristics and risk factors for cervical cancer. Molecular screening for HPV16, HPV18 and 12 other high-risk HPV types was performed on cervical and oral rinse specimens using Cobas 4800 (Roche Molecular Systems, USA).ResultsHPV was detected in the oral rinse of 3.9% of participants. Infection of the oral cavity with a non-HPV16 or 18 type was most frequent (81.2%), followed by HPV16 (18.7%). Infection with HPV in the cervix and oral cavity was present in 11 (2.7%) of participants. There were no differences observed in the smoking status (p value 0.62), mean age of first sexual intercourse (p value 0.25), mean age of the first oral sex (p value 0.90) or mean lifetime number of sexual partners (p value 0.08) between the participants with oral HPV infection or not.ConclusionThe presence of HPV infection in the oral cavity was low in the group of women with abnormal cervical cancer screening findings and a high rate of cervical HPV infection.

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Human papillomavirus vaccination coverage among working women

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v6i4.51238 ◽

2021 ◽

Vol 6 (4) ◽

pp. 714-722

Author(s):

Sajeda Khatun ◽

Ashees Kumar Saha ◽

Irtiza Ahmed Chowdhury ◽

Arifa Sultana ◽

Mst Wazeda Begum ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Vaccination Coverage ◽

Hpv Vaccination ◽

Working Women ◽

Hpv Infection ◽

Cross Sectional Study ◽

P Value ◽

Human Papillomavirus Vaccination ◽

Cross Sectional

Human papillomavirus (HPV) infection is one of the causes of cervical cancer which is preventable. The study was a cross-sectional study with the objective was to determine the Human papillomavirus (HPV) vaccination coverage among working women. A total 236 educated working women were selected purposively from different working places in Dhaka city. A pretested semi- structured questionnaire was used for data collection. The knowledge was assessed by using modified Blooms cut off points which was developed with cervical cancer and vaccine knowledge related questions. The study result revealed that among 236 working women 71.2% knew about the risk factor of cervical cancer. It was found that 92.8% knew about the way of prevention of cervical cancer respectively. The overall knowledge on cervical cancer and vaccine was found to be having good knowledge 21.2%, satisfactory knowledge 41.9% and poor knowledge 36.9%. Out of the total working women only 5.1% had HPV vaccination coverage. Reason behind non vaccinations, 50% mentioned had no knowledge about vaccine, 35.3% mentioned can’t decide and 8.9% mentioned high cost of vaccine. Significant association has been observed in between vaccination coverage with over all knowledge of cervical cancer (p value .04). It may be concluded that HPV vaccination coverage is very low which needs awareness program on this issues through mass media, educational session, counseling and so on to reduce human papillomavirus associated cancers as well as economic burden. Asian J. Med. Biol. Res. December 2020, 6(4): 714-722

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Distribution and Prevalence of High-Risk Human Papillomavirus Genotypes in Cervical Specimens

Flora the Journal of Infectious Diseases and Clinical Microbiology ◽

10.5578/flora.68958 ◽

2020 ◽

Vol 25 (3) ◽

pp. 325-331

Author(s):

Erkan Özmen ◽

Ülkü Altoparlak ◽

Muhammet Hamidullah Uyanık ◽

Abdulkadir Gülen

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Age Groups ◽

Hpv Infection ◽

High Rate ◽

Genotype Distribution ◽

Hpv Dna ◽

Hpv Test ◽

Significant Difference ◽

Hpv Types

Introduction: Human papillomavirus (HPV) is frequently a sexually transmitted virus and can cause cervical cancer in women. Cervical cancer is the second most common type of cancer among the developing countries. In this study, cervical HPV DNA positivity and genotype distributions were investigated in female patients living in our region and the results were compared with different studies. Materials and Methods: Between 1 July, 2017 and 1 March, 2019, 433 cervical swabs were sent to Ataturk University, Medical Faculty Hospital, Medical Microbiology Laboratory due to suspicion of HPV. Swab samples were evaluated for HPV virus using molecular (Polymerase Chain Reaction-PCR) methods. For this purpose, Xpert HPV Test (Cepheid, Inc, Sunnyvale, CA) was used to identify HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 t in a single sample. Results: Mean age of the patients ranged from 20 to 69 years, with a mean of 39.8 years (± 10.0). Positivity was detected in 62 of the 433 patients. Mean age of the positive patients was 40.2 years (± 11.3). When the positive patients were examined in terms of HPV types, the presence of HPV 16 was observed with a rate of 25.6%, while the HPV 18/45 types were found to be 9.0% in total. When patients were evaluated according to age groups, HPV DNA positivity was highest in the 25-34 age group with 38.7%. In our statistical study, there was no significant difference in HPV DNA positivity rate between the ages of 35 and under 35 years. Conclusion: This study demonstrates the prevalence and viral genotype distribution of HPV infection in women in Erzurum region. HPV type 16 is seen with a high rate in our region.

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Characteristics of high-risk human papillomavirus infection in women with abnormal cervical cytology: a population-based study in Shanxi Province, China

10.21203/rs.2.15784/v1 ◽

2019 ◽

Author(s):

Li Song ◽

Yuanjing Lyu ◽

Ling Ding ◽

Xiaoxue Li ◽

Wen Gao ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

Cervical Cytology ◽

Shanxi Province ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Abnormal Cervical Cytology ◽

Intraepithelial Lesion

Abstract Background: High-risk human papillomavirus (HR-HPV) infection is widely known as the major cause of cervical intraepithelial neoplasia (CIN) and cervical cancer and it’s characteristics vary greatly in different population. Women with abnormal cervical cytology could increase the risk of cervical cancer, however, HR-HPV infection characteristics in women with abnormal cervical cytology remains unclear. Methods: This study was based on baseline survey of the CIN Cohort established in Shanxi Province, China. A total number of 2300 women with cervical abnormalities were enrolled in this study. All participants gave informed consent and agreed to HPV and thinprepcytologic test (TCT). Each individual completed a questionnaire about characteristics related to HPV infection. Results: The overall prevalence of HR-HPV in 2300 women was 32.0%, and the proportion of single and multiple HR-HPV infections were 70.2% and 29.8% in HR-HPV infection women, respectively. The top five HR-HPV genotypes were ranked as HPV16, HPV58, HPV52, HPV53 and HPV51. The prevalence of HR-HPV in atypical squamous cells of undetermined significance (ASC-US), low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion and above(HSIL+) were 30.8%, 36.5% and 54.9%, respectively, showing an increasing trend with the severity of cervical cytology ( χ 2 trend =13.952; p <0.001). The women aged 35~45 years, with lower education level, less frequency of bathing, multiple gravidity, multiple parity, history of gynecological diseases and premenopausal women were prone to HR-HPV infection. Conclusions: We defined the characteristics related to HR-HPV infection in abnormal cervical cytology women, and provided an insight for the development and deeply research of HPV vaccine.

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Squamous lesions with glandular involvement: conventional cytology versus liquid-based cytology

Citotech Online - Case Review ◽

10.26537/citotech.v0i3.2284 ◽

2017 ◽

Author(s):

Telma Oliveira ◽

Vera Azevedo ◽

Rute Dominguez

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

European Country ◽

Incidence Rate ◽

Screening Method ◽

Hpv Infection ◽

Cervical Cytology ◽

Western European Country ◽

Conventional Cytology ◽

Liquid Based Cytology

Portugal is the Western European country with the highest incidence rate of cervical cancer, being the Human Papillomavirus (HPV) infection its main cause. Cervical cytology is the most widely used screening method for the prevention and detection of cervical cancer, either by conventional means or by liquid basis. Conventional cytological preparations have more limitations than liquid-based preparations, however, when dealing with glandular lesions, both conventional and liquid-based cytology have some drawbacks. We report the case of a 31-year-old woman whose results from the screening cytology tests diverge from histological diagnosis. With this study we intend to highlight the limitations of conventional cytology in relation to the liquid-based cytology in the diagnostic of glandular lesions.

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Concordance of Human Papillomavirus Type 16 and 18 in Cervical and Oral Specimen of Cervical Cancer Patients

Indonesian Journal of Obstetrics and Gynecology ◽

10.32771/inajog.v7i1.817 ◽

2019 ◽

pp. 70

Author(s):

Willy Akbar ◽

Syahrul Rauf ◽

Deviana S. Riu ◽

St. Maisuri T. Chalid

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Oral Cavity ◽

Cancer Patients ◽

Oral Fluid ◽

Cross Sectional Study ◽

Cross Sectional ◽

Hpv 16 ◽

Human Papillomavirus Type 16 ◽

Oral Hpv

Abstract Objective : To determine the conformity of HPV type 16 and 18 in cervical and oral/buccal specimens from cervical cancer patients. Methods :A cross-sectional study was conducted in March - September 2016 at several hospitals in Makassar. HPV 16 and 18 genotyping in cervical and oral fluid of 77 patients with cervical cancer performed with PCR method. Results : The prevalence of HPV type 18 infection both in the cervical and the oral fluid was higher than HPV type 16 [9(47.4%) vs 5(26.3%)]. The aggreement of HPV type 18 infection (r=0.328;p=0.000) in the cervical-oral sites was higher than HPV type 16 (r=0.194;p=0.042). Conclusion : HPV type 16 and 18 could infect both cervix and oral cavity although type-specific concordance is low. Keywords :Human papillomavirus,servix, oral cavity Abstrak Tujuan: Mengetahui tingkat kesesuaian hasil pemeriksaan HPV tipe 16 dan 18 antara spesimen serviks dan oral/buccal pada penderita kanker serviks. Metode: Penelitian cross sectional ini dilakukan pada Maret – September 2016 pada beberapa rumah sakit di Makassar. Pemeriksaan HPV 16 dan 18 pada cairan serviks dan oral dari 77 orang penderita kanker serviks menggunakan teknik PCR. Hasil: Prevalensi infeksi bersama pada serviks dan oral HPV tipe 18 lebih tinggi dibandingkan HPV tipe 16 [9(47,4%) vs 5(26,3%)]. Tingkat kesesuaian antara HPV tipe 18 (r=0,328;p=0,000) pada serviks dan oral lebih tinggi dibandingkan tipe 16 (r=0,194;p=0,042). Kesimpulan: HPV tipe 16 dan 18 dapat menginfeksi serviks dan oral meskipun tingkat kesesuaian kedua tipe ini lemah. Kata kunci : Human papillomavirus, serviks, kavum oral

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Interaction of High- and Low-Risk human papillomavirus genotypes is associated with a reduced risk of developing cervical cancer (Preprint)

10.2196/preprints.36017 ◽

2021 ◽

Author(s):

Amir Avan

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

Population Based ◽

High Risk Group ◽

Low Risk ◽

P Value ◽

Simultaneous Infection ◽

High Risk Hpv

BACKGROUND Cervical cancer is among the most common type of cancers in women and is associated with human papillomavirus (HPV) infection. OBJECTIVE The link between cervical cancer and high-risk HPV infection has been well documented, although the effect of simultaneous infection with high- and low-risk HPV or low-risk HPV alone on the risk of developing cervical malignancy is remained to be unanswered in guideline METHODS We have investigated the association of high and low-risk HPVs (HR or LR) genotype with cervical carcinoma risk, as well as pathological and cytological information in cases recruited from a population-based cohort study of 790 patients. RESULTS The percentage of HR+LR and HR-HPV16/18 were 9.30% and 11.20% in class II, 7.15% and 7.10% in class IV and 7.15% and 5.80% in As-CUS smears. Interestingly concurrent infection with HR-HPV and LR-HPV types led to a notable decline in the risk of developing malignancy in comparison with the high-risk group (OR=0.3 (0.098-0.925), p-value=0.04). The percentage of individuals with cervical malignancy was 10.2% and 28.2% within the co-infected and the HR-HPV participants. CONCLUSIONS Our finding demonstrated that simultaneous infection with high- and low-risk HPV reduces the risk of cervical malignancy.

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Oral human papillomavirus infection in HIV-infected and HIV-uninfected MSM: the OHMAR prospective cohort study

Sexually Transmitted Infections ◽

10.1136/sextrans-2019-054301 ◽

2020 ◽

Vol 96 (7) ◽

pp. 528-536 ◽

Cited By ~ 2

Author(s):

Massimo Giuliani ◽

Francesca Rollo ◽

Maria Fenicia Vescio ◽

Barbara Pichi ◽

Alessandra Latini ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

Incidence Rates ◽

Oral Sex ◽

Sex Partners ◽

Oral Rinse ◽

Increased Risk ◽

Frequent Event ◽

Oral Hpv

ObjectiveWe aimed to assess incidence and clearance of oral human papillomavirus (HPV) infection and the respective risk factors in HIV-infected and uninfected men who have sex with men (MSM).MethodsOral rinse and gargles were collected semiannually from 244 MSM (103, 42.2% HIV-infected). HPV-DNA testing was performed with the Linear Array HPV Genotyping test. A Markov model was used for estimation of incidence, clearance and risk factor analysis.ResultsIncidence rates for any HPV were 21.2 and 15.0×1000 person-months in HIV-infected and uninfected MSM, respectively. The respective figures for high-risk HPVs were 10.7 and 6.5×1000 person-months. The clearance rate was 4–12 times higher than the respective incidence rate. HIV-infected MSM with >95 lifetime oral sex partners showed increased incidence of any HPV (adjusted HR, aHR: 8.46, 95% CI 1.89 to 37.92). Condomless oral sex appeared the strongest predictor for incident infection by high-risk HPVs in this group (aHR: 13.40, 95% CI 2.55 to 70.53). Those aged >46 years (aHR: 0.30, 95% CI 0.12 to 0.74) and those with nadir CD4+ T count of <200 cells/mm3 (aHR: 0.14, 95% CI 0.03 to 0.75) displayed a significantly reduced clearance of any and high-risk HPVs, respectively. HIV-uninfected MSM aged >46 years had increased risk of acquiring any HPV (aHR: 3.70, 95% CI 1.30 to 10.52) and high-risk HPV (aHR: 5.33, 95% CI 1.06 to 26.68). Any HPV clearance declined in those with more than six recent oral sex partners (aHR: 0.18, 95% CI 0.05 to 0.65).ConclusionsAcquisition of oral HPV infection in MSM seems to occur rarely, whereas clearance seems to be a frequent event. Oral HPV natural history in these at-risk subjects is differently influenced by age and sex behaviour, depending on HIV status.

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Prevalence and genotype specific concordance of oro-genital and anal human papillomavirus infections among sexually active Nigerian women

Infectious Agents and Cancer ◽

10.1186/s13027-021-00398-9 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Imran O. Morhason-Bello ◽

Kathy Baisley ◽

Miquel Angel Pavon ◽

Isaac F. Adewole ◽

Rasheed Bakare ◽

...

Keyword(s):

Risk Factors ◽

Human Papillomavirus ◽

Oral Cavity ◽

Hpv Infection ◽

Cross Sectional Study ◽

Sub Saharan Africa ◽

Sexually Active ◽

Cross Sectional ◽

Nigerian Women ◽

Oral Hpv

Abstract Background Human papillomavirus (HPV) associated cancers are increasingly reported globally, including in sub-Saharan Africa (SSA). However, with the exception of cervical HPV infection, data from SSA on the epidemiology of oral and genital HPV infections are limited. This study assessed the prevalence and concordance of oro-genital and anal HPV genotype specific infections among women in the general population. Methods We conducted a cross-sectional study in sexually active women aged 18–45 years in Ibadan, Nigeria. After a face-to-face interview and clinical examination, oral, cervical, vulvar, and anal samples were collected from participants and tested by the Anyplex II 28 HPV assay. Descriptive and multivariable analyses were used to report prevalence and risk factors associated with HPV infections. Results The prevalence of any vulva, cervical, anal, and oral HPV infections was 68.0% (210/309), 59.7% (182/305), 56.8% (172/303), and 16.1% (14/286), respectively. There was an inverse relationship between age-group and HPV prevalence of HPV in all anatomic sites except for the oral HPV infections. HPV 35 was the most prevalent high-risk HPV genotype in the vulva, cervix and oral cavity. Associated risk factors for HPV infection in each of the anatomic sites were reported. Overall, 10.0% (31/310) women had concordance of any HPV type in the four anatomic sites. Conclusion There was a high prevalence of oro-genital and anal HPV infections among sexually active Nigerian women, with concordance of HPV types in the cervix, vulva, anus and oral cavity. We advocate large longitudinal studies that will involve sampling of multiple anatomic sites and inclusion of other women in the community for better understanding of HPV epidemiology in this region.

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Knowledge of Human Papillomavirus and Uptake of its Vaccine among Female Undergraduate Students of Ambrose Alli University, Ekpoma, Nigeria

Journal of Community Medicine and Primary Health Care ◽

10.4314/jcmphc.v33i1.6 ◽

2021 ◽

Vol 33 (1) ◽

pp. 64-75

Author(s):

A.R. Isara ◽

N. Osayi

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Undergraduate Students ◽

Hpv Vaccine ◽

Sexual Debut ◽

Statistical Significance ◽

Hpv Infection ◽

Vaccine Uptake ◽

P Value ◽

Female Undergraduate

Background: Human Papillomavirus (HPV) is a prerequisite for the development of cervical cancer which is the fourth most common cancer affecting women worldwide. The use of HPV vaccine has been found to be responsible for significant decline in the prevalence of HPV infection and consequently, of cervical cancer. This study assessed the knowledge of HPV and the uptake of HPV vaccine among female undergraduate students of Ambrose Alli University, Ekpoma, Nigeria.Methods: This institution-based cross-sectional study was carried out among 229 students selected using multi-staged sampling technique. A structured, pretested, self-administered questionnaire was used to collect data. IBM SPSS version 21.0 was used for data analysis. Statistical significance was set at p-value less than 0.05.Results: Only 12 (5.2%) students were knowledgeable about HPV and 39 (17.0%) were aware of the existence of HPV vaccines. The participants’ age (p = 0.031) and level of study (p = 0.026) were significantly associated with knowledge of HPV. Only 1 (0.44%) student had received the vaccine. Eight (10.8%) students had their sexual debut at 10 to 14 years, 9 (12.1%) had more than five sexual partners and 21 (23.4%) never used condom during sexual intercourse.Conclusion: The knowledge about HPV infection and uptake of HPV vaccine were very low in this study. Concerted effort should be made by health authorities to create awareness about HPV infection and its vaccine among university students. This will improve HPV vaccine uptake, prevent HPV infections and reduce the prevalence of cervical cancer.

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High Rate of Infection by Only Oncogenic Human Papillomavirus in Amerindians

mSphere ◽

10.1128/msphere.00176-18 ◽

2018 ◽

Vol 3 (3) ◽

Cited By ~ 6

Author(s):

Daniela Vargas-Robles ◽

Magda Magris ◽

Natalia Morales ◽

Maurits N. C. de Koning ◽

Iveth Rodríguez ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

High Rate ◽

Human Populations ◽

Viral Agent ◽

Hpv Types ◽

Amazonas State ◽

The Town

ABSTRACTHuman papillomavirus (HPV), an etiological agent of cervical cancer (CC), has infected humans since ancient times. Amerindians are the furthest migrants out of Africa, and they reached the Americas more than 14,000 years ago. Some groups still remain isolated, and some migrate to towns, forming a gradient spanning urbanization. We hypothesized that, by virtue of their history, lifestyle, and isolation from the global society, remote Amerindian women have lower HPV diversity than do urban women (Amerindian or mestizo). Here we determined the diversity of the 25 most relevant cervical HPV types in 82 Amerindians spanning urbanization (low, medium, and high, consistent with the exposure to urban lifestyles of the town of Puerto Ayacucho in the Venezuelan Amazonas State), and in 29 urban mestizos from the town. Cervical, anal, oral, and introitus samples were taken, and HPVs were typed using reverse DNA hybridization. A total of 23 HPV types were detected, including 11 oncogenic or high-risk types, most associated with CC. Cervical HPV prevalence was 75%, with no differences by group, but Amerindians from low and medium urbanization level had significantly lower HPV diversity than mestizos did. In Amerindians, but not in mestizos, infections by only high-risk HPVs were higher than coinfections or by exclusively low-risk HPVs. Cervical abnormalities only were observed in Amerindians (9/82), consistent with their high HPV infection. The lower cervical HPV diversity in more isolated Amerindians is consistent with their lower exposure to the global pool, and transculturation to urban lifestyles could have implications on HPV ecology, infection, and virulence.IMPORTANCEThe role of HPV type distribution on the disparity of cervical cancer (CC) incidence between human populations remains unknown. The incidence of CC in the Amazonas State of Venezuela is higher than the national average. In this study, we determined the diversity of known HPV types (the viral agent of CC) in Amerindian and mestizo women living in the Venezuelan Amazonas State. Understanding the ecological diversity of HPV in populations undergoing lifestyle transformations has important implication on public health measures for CC prevention.

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