Concordance of Human Papillomavirus Type 16 and 18 in Cervical and Oral Specimen of Cervical Cancer Patients

Abstract Objective : To determine the conformity of HPV type 16 and 18 in cervical and oral/buccal specimens from cervical cancer patients. Methods :A cross-sectional study was conducted in March - September 2016 at several hospitals in Makassar. HPV 16 and 18 genotyping in cervical and oral fluid of 77 patients with cervical cancer performed with PCR method. Results : The prevalence of HPV type 18 infection both in the cervical and the oral fluid was higher than HPV type 16 [9(47.4%) vs 5(26.3%)]. The aggreement of HPV type 18 infection (r=0.328;p=0.000) in the cervical-oral sites was higher than HPV type 16 (r=0.194;p=0.042). Conclusion : HPV type 16 and 18 could infect both cervix and oral cavity although type-specific concordance is low. Keywords :Human papillomavirus,servix, oral cavity Abstrak Tujuan: Mengetahui tingkat kesesuaian hasil pemeriksaan HPV tipe 16 dan 18 antara spesimen serviks dan oral/buccal pada penderita kanker serviks. Metode: Penelitian cross sectional ini dilakukan pada Maret – September 2016 pada beberapa rumah sakit di Makassar. Pemeriksaan HPV 16 dan 18 pada cairan serviks dan oral dari 77 orang penderita kanker serviks menggunakan teknik PCR. Hasil: Prevalensi infeksi bersama pada serviks dan oral HPV tipe 18 lebih tinggi dibandingkan HPV tipe 16 [9(47,4%) vs 5(26,3%)]. Tingkat kesesuaian antara HPV tipe 18 (r=0,328;p=0,000) pada serviks dan oral lebih tinggi dibandingkan tipe 16 (r=0,194;p=0,042). Kesimpulan: HPV tipe 16 dan 18 dapat menginfeksi serviks dan oral meskipun tingkat kesesuaian kedua tipe ini lemah. Kata kunci : Human papillomavirus, serviks, kavum oral

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Cytotoxic T-Lymphocyte Responses to Human Papillomavirus Type 16 E5 and E7 Proteins and HLA-A*0201-Restricted T-Cell Peptides in Cervical Cancer Patients

Journal of Virology ◽

10.1128/jvi.02256-06 ◽

2007 ◽

Vol 81 (6) ◽

pp. 2869-2879 ◽

Cited By ~ 14

Author(s):

Dai-Wei Liu ◽

Yuh-Cheng Yang ◽

Ho-Fan Lin ◽

Mei-Fang Lin ◽

Ya-Wen Cheng ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

T Cell ◽

Cancer Patients ◽

T Lymphocyte ◽

Cytotoxic T Lymphocyte ◽

Hpv 16 ◽

Human Papillomavirus Type 16 ◽

E5 Protein ◽

E7 Proteins

ABSTRACT Previously, we found that human papillomavirus type 16 (HPV-16) E5 protein is a tumor rejection antigen and can induce cytotoxic T-lymphocyte (CTL) activity. Therefore, in this study, human leukocyte antigen A*0201 (HLA-A*0201)-restricted human CTL epitopes of HPV-16 E5 protein were identified using a bioinformatics approach, and the abilities of these predicted peptides to induce an immune response in HLA-A*0201 transgenic mice were confirmed by assaying E5-specific CTLs and in vitro-generated CTLs from normal peripheral blood T lymphocytes of HLA-A2-positive human donors. Second, the CTL responses to HLA-A*0201 CTL epitopes (E5 63-71 and E7 11-20) were examined in HPV-16-infected patients with HLA-A2. Third, the effect of HLA-A-type alleles on CTL activities in response to the entire E5 and E7 proteins was examined in cervical cancer patients. E5 and E7 peptides (but not the whole proteins) stimulated E5- and E7-specific CTL recall responses in HPV-16- and HLA-A2-positive cervical cancer patients, and HPV-16 E5 and E7 proteins stimulated naïve T cells in HPV-16-negative cervical cancer patients with HLA-A11 and -A24 haplotypes. In summary, this is the first demonstration that E5 63-71 is an HLA-A*0201-restricted T-cell epitope of HPV-16 E5.

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Prevalence and genotype specific concordance of oro-genital and anal human papillomavirus infections among sexually active Nigerian women

Infectious Agents and Cancer ◽

10.1186/s13027-021-00398-9 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Imran O. Morhason-Bello ◽

Kathy Baisley ◽

Miquel Angel Pavon ◽

Isaac F. Adewole ◽

Rasheed Bakare ◽

...

Keyword(s):

Risk Factors ◽

Human Papillomavirus ◽

Oral Cavity ◽

Hpv Infection ◽

Cross Sectional Study ◽

Sub Saharan Africa ◽

Sexually Active ◽

Cross Sectional ◽

Nigerian Women ◽

Oral Hpv

Abstract Background Human papillomavirus (HPV) associated cancers are increasingly reported globally, including in sub-Saharan Africa (SSA). However, with the exception of cervical HPV infection, data from SSA on the epidemiology of oral and genital HPV infections are limited. This study assessed the prevalence and concordance of oro-genital and anal HPV genotype specific infections among women in the general population. Methods We conducted a cross-sectional study in sexually active women aged 18–45 years in Ibadan, Nigeria. After a face-to-face interview and clinical examination, oral, cervical, vulvar, and anal samples were collected from participants and tested by the Anyplex II 28 HPV assay. Descriptive and multivariable analyses were used to report prevalence and risk factors associated with HPV infections. Results The prevalence of any vulva, cervical, anal, and oral HPV infections was 68.0% (210/309), 59.7% (182/305), 56.8% (172/303), and 16.1% (14/286), respectively. There was an inverse relationship between age-group and HPV prevalence of HPV in all anatomic sites except for the oral HPV infections. HPV 35 was the most prevalent high-risk HPV genotype in the vulva, cervix and oral cavity. Associated risk factors for HPV infection in each of the anatomic sites were reported. Overall, 10.0% (31/310) women had concordance of any HPV type in the four anatomic sites. Conclusion There was a high prevalence of oro-genital and anal HPV infections among sexually active Nigerian women, with concordance of HPV types in the cervix, vulva, anus and oral cavity. We advocate large longitudinal studies that will involve sampling of multiple anatomic sites and inclusion of other women in the community for better understanding of HPV epidemiology in this region.

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Nurses’ and midwives’ knowledge, attitudes, and acceptance regarding human papillomavirus vaccination in Ghana: a cross-sectional study

BMC Nursing ◽

10.1186/s12912-020-00530-x ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Nancy Innocentia Ebu ◽

Gifty Esinam Abotsi-Foli ◽

Doreen Faakonam Gakpo

Keyword(s):

Risk Factors ◽

Cervical Cancer ◽

Human Papillomavirus ◽

Hpv Vaccination ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Cancer Risk Factors ◽

Cervical Cancer Risk ◽

Nurses And Midwives

Abstract Background Nurses and midwives play important roles in educating the public on cervical cancer prevention strategies. Aim This study sought to assess nurses’ and midwives’ knowledge of, attitudes towards, and acceptance of human papillomavirus (HPV) vaccination in relation to their background characteristics. Methods A descriptive cross-sectional study using questionnaires was conducted with a convenience sample of 318 female nurses and midwives, ages 20 to 59, at the Korle-Bu Teaching Hospital in Ghana. The data were summarised using frequencies, percentages, chi-square tests, and Fisher’s exact tests. Results The results indicated that 41.5% (n = 132) of the participants had high levels of knowledge about cervical cancer risk factors, and 17.6% (n = 56) of the respondents had received at least one dose of the HPV vaccine. Reasons for receiving the HPV vaccination included advice from a colleague (12.9%, n = 41) and perceived threat of cervical cancer (11.7%, n = 37). Of the 262 respondents who had not been vaccinated, 24.45% (n = 78) strongly agreed and 28.0% (n = 89) agreed with the statement that there was limited information on HPV vaccination. Also, there were statistically significant associations between age (X2 = 23.746, p = 0.001), marital status (X2 = 14.758, p = 0.005), completed level of education (X2 = 21.692, p = 0.001), and duration of working at the hospital (X2 = 8.424, p = 0.038) and acceptance of HPV vaccination. Conclusions This study demonstrated gaps in knowledge about cervical cancer risk factors and attitudes towards HPV vaccination, indicating the need for targeted measures to improve knowledge and attitudes. Also, measures to increase acceptance of HPV vaccination among nurses and midwives should consider their sociodemographic characteristics.

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Multiple ASF/SF2 Sites in the Human Papillomavirus Type 16 (HPV-16) E4-Coding Region Promote Splicing to the Most Commonly Used 3′-Splice Site on the HPV-16 Genome

Journal of Virology ◽

10.1128/jvi.00462-10 ◽

2010 ◽

Vol 84 (16) ◽

pp. 8219-8230 ◽

Cited By ~ 33

Author(s):

Monika Somberg ◽

Stefan Schwartz

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Splice Site ◽

Binding Sites ◽

Mrna Levels ◽

Coding Region ◽

Cervical Lesions ◽

Hpv 16 ◽

Human Papillomavirus Type 16 ◽

E6 And E7

ABSTRACT Our results presented here demonstrate that the most abundant human papillomavirus type 16 (HPV-16) mRNAs expressing the viral oncogenes E6 and E7 are regulated by cellular ASF/SF2, itself defined as a proto-oncogene and overexpressed in cervical cancer cells. We show that the most frequently used 3′-splice site on the HPV-16 genome, site SA3358, which is used to produce primarily E4, E6, and E7 mRNAs, is regulated by ASF/SF2. Splice site SA3358 is immediately followed by 15 potential binding sites for the splicing factor ASF/SF2. Recombinant ASF/SF2 binds to the cluster of ASF/SF2 sites. Mutational inactivation of all 15 sites abolished splicing to SA3358 and redirected splicing to the downstream-located, late 3′-splice site SA5639. Overexpression of a mutant ASF/SF2 protein that lacks the RS domain, also totally inhibited the usage of SA3358 and redirected splicing to the late 3′-splice site SA5639. The 15 ASF/SF2 binding sites could be replaced by an ASF/SF2-dependent, HIV-1-derived splicing enhancer named GAR. This enhancer was also inhibited by the mutant ASF/SF2 protein that lacks the RS domain. Finally, silencer RNA (siRNA)-mediated knockdown of ASF/SF2 caused a reduction in spliced HPV-16 mRNA levels. Taken together, our results demonstrate that the major HPV-16 3′-splice site SA3358 is dependent on ASF/SF2. SA3358 is used by the most abundantly expressed HPV-16 mRNAs, including those encoding E6 and E7. High levels of ASF/SF2 may therefore be a requirement for progression to cervical cancer. This is supported by our earlier findings that ASF/SF2 is overexpressed in high-grade cervical lesions and cervical cancer.

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Human papillomavirus vaccination coverage among working women

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v6i4.51238 ◽

2021 ◽

Vol 6 (4) ◽

pp. 714-722

Author(s):

Sajeda Khatun ◽

Ashees Kumar Saha ◽

Irtiza Ahmed Chowdhury ◽

Arifa Sultana ◽

Mst Wazeda Begum ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Vaccination Coverage ◽

Hpv Vaccination ◽

Working Women ◽

Hpv Infection ◽

Cross Sectional Study ◽

P Value ◽

Human Papillomavirus Vaccination ◽

Cross Sectional

Human papillomavirus (HPV) infection is one of the causes of cervical cancer which is preventable. The study was a cross-sectional study with the objective was to determine the Human papillomavirus (HPV) vaccination coverage among working women. A total 236 educated working women were selected purposively from different working places in Dhaka city. A pretested semi- structured questionnaire was used for data collection. The knowledge was assessed by using modified Blooms cut off points which was developed with cervical cancer and vaccine knowledge related questions. The study result revealed that among 236 working women 71.2% knew about the risk factor of cervical cancer. It was found that 92.8% knew about the way of prevention of cervical cancer respectively. The overall knowledge on cervical cancer and vaccine was found to be having good knowledge 21.2%, satisfactory knowledge 41.9% and poor knowledge 36.9%. Out of the total working women only 5.1% had HPV vaccination coverage. Reason behind non vaccinations, 50% mentioned had no knowledge about vaccine, 35.3% mentioned can’t decide and 8.9% mentioned high cost of vaccine. Significant association has been observed in between vaccination coverage with over all knowledge of cervical cancer (p value .04). It may be concluded that HPV vaccination coverage is very low which needs awareness program on this issues through mass media, educational session, counseling and so on to reduce human papillomavirus associated cancers as well as economic burden. Asian J. Med. Biol. Res. December 2020, 6(4): 714-722

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Pathways to care and diagnostic delay among cervical cancer patients attending a tertiary care hospital in Puducherry: a cross-sectional study

International Journal of Community Medicine and Public Health ◽

10.18203/2394-6040.ijcmph20202489 ◽

2020 ◽

Vol 7 (6) ◽

pp. 2298

Author(s):

Roshina Sunny ◽

Sitanshu Sekhar Kar ◽

Dasari Papa ◽

Sujiv Akkilagunta ◽

Jeby Jose Olickal

Keyword(s):

Cervical Cancer ◽

Cancer Patients ◽

Tertiary Care ◽

Diagnostic Delay ◽

Cross Sectional Study ◽

Cancer Center ◽

Care Hospital ◽

Sectional Study ◽

Cross Sectional ◽

Care Facilities

Background: The high mortality among cervical cancer patients in India can be attributed to presentation at advanced stages. The varied and lengthy pathway taken up to diagnosis could be a major reason for advanced stage at presentation. Hence, we aimed to describe the care pathways and diagnostic delay among cervical cancer patients.Methods: A hospital-based cross-sectional study was conducted among 101 cervical cancer patients attending a cancer clinic at a Regional Cancer Center. The histo-pathologically confirmed cases of cervical cancer, who registered in July to October 2018 were approached. Data were collected through personal interviews using a semi-structured questionnaire. Descriptive statistics were used to describe the number of providers visited and diagnostic delay.Results: The median (range) number of providers visited by the patients up to diagnosis was 2 (1-5). As the first point of care, 14% of participants approached sub-center or primary care facilities, 27% approached secondary care facilities, 49% participants approached tertiary care facilities and 11% came directly to a regional cancer center. Diagnosis was made only in 24% of participants at secondary and tertiary care levels. The median (IQR) number of days to get diagnosed was 66 (30-130) days and three fourth of the patients had a diagnostic delay.Conclusions: The diagnostic delay was higher among patients who consulted multiple providers. Implementing a protocol to be followed at all three levels of health care delivery system may enhance the early diagnosis.

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Prognostic implication of human papillomavirus types in cervical cancer patients: a systematic review and meta-analysis

Infectious Agents and Cancer ◽

10.1186/s13027-020-00332-5 ◽

2020 ◽

Vol 15 (1) ◽

Author(s):

Yuanyuan Xu ◽

Yichao Qiu ◽

Shuang Yuan ◽

Hongjing Wang

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Cancer Patients ◽

Meta Analysis ◽

Disease Free Survival ◽

Progression Free Survival ◽

Hpv 16 ◽

Free Survival ◽

American Society ◽

Hpv 18

Abstract Background To estimate the prognostic relevance of human papillomavirus (HPV) 16 and HPV 18 in patients with cervical cancer. Method We searched PubMed, EMBASE, American Society of Clinical Oncology (ASCO) and the European Society of Medical Oncology (ESMO), CNKI, and Wanfang databases to search primary articles illustrating the survival outcomes in cervical cancer patients with or without HPV 16/18 infection. A meta-analysis was conducted to generate a combined hazard ratio (HR) with 95% confidence intervals (CI) for progression-free survival (PFS), disease free survival (DFS) and overall survival (OS). Results A total of 13 studies were included. Our meta-analysis revealed that HPV 16 positive did not have any impact on OS (HR, 0.76; 95% CI = 0.37–1.54; P = 0.44). Cervical cancer patiensts infected with HPV 18 had worse OS (HR, 1.66; 95% CI = 1.28–2.17; P = 0.0001), DFS (HR, 2.10; 95% CI = 1.73–2.54; P < 0.0001) and worse PFS (HR, 2.97; 95% CI = 1.69–5.23; P = 0.00012) compared with those not infected with HPV 18. cervical cancer patiensts infected with HPV 18 had worse PFS compared with those infected with HPV 16 ((HR, 1.34; 95% CI = 1.06–1.70; P = 0.01). Conclusion Cervical cancer patients infected with HPV 18 had worse survival compared with cervical cancer patients with HPV 16 infection.

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High prevalent human papillomavirus infections of the oral cavity of asymptomatic HIV-positive men

BMC Infectious Diseases ◽

10.1186/s12879-019-4677-9 ◽

2020 ◽

Vol 20 (1) ◽

Cited By ~ 1

Author(s):

Rocío Méndez-Martínez ◽

Silvia Maldonado-Frías ◽

Salvador Vázquez-Vega ◽

Yanink Caro-Vega ◽

José Guadalupe Rendón-Maldonado ◽

...

Keyword(s):

Human Papillomavirus ◽

Oral Cavity ◽

Anal Canal ◽

Cross Sectional Study ◽

Cross Sectional ◽

Oral Infections ◽

Immunodeficiency Virus ◽

High Prevalence ◽

Sex With Men ◽

High Risk Hpv

Abstract Background Incidence of anal and oral infections with Human Papillomavirus (HPV) is increasing, particularly among Human Immunodeficiency Virus-positive (HIV+) men. HPV type 16 has exhibited the highest incidence and only limited data is available on other prevalent types, variants of HPV16, as well as associated factors. We were interested in identifying prevalent HPV types, variants of type 16, as well as factors associated with HPV16 infections in the oral cavity of HIV+ men who have sex with men (MSM). Methods A cross-sectional study of oral cavity samples from HIV+ MSM, that in a previous study were identified as positive for HPV16 in the anal canal. Cells from the oral cavity (102 samples, paired with 102 from the anal canal of same patient) were used to extract DNA and detect HPV infections using INNO-LiPA HPV Genotyping Extra II, and PCR. From these, 80 samples (paired, 40 anal and 40 oral) were used to identify variants of type 16 by sequencing. Statistical differences were estimated by the X2 test, and p values equal to or less than 0.05 were considered significant. SPSS ver. Twenty-four statistical software (IBM Corp) was used. Results We found a high prevalence of High-Risk HPV (HR-HPV) and Low-Risk HPV (LR-HPV). Patients were positive in the oral cavity for HR types; 16, 39 and 18 (80.4, 61.8 and 52.9% respectively) and LR types 11 and 6 (53.9 and 34.3% respectively). Surprisingly, only European variants of type 16 were found in the oral cavity, although American Asian (22.5%) and African (2.5%) variants were identified in the anal canal. The analysis showed that CD4 counts could be the most important risk factor associated with HR-HPV infections in the oral cavity, anal canal or both anatomical regions. The risk of infection of the oral cavity with type 18 increased in men diagnosed with HIV for more than 6 years. Conclusions Prevalence of both HR and LR HPV’s in the oral cavity of Mexican HIV+ MSM is very high. The fact that only European variants of HPV16 were found in the oral cavity suggest a possible tropism not previously described.

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