Infectious keratoconjunctivitis and occurrence ofMycoplasma conjunctivaeand Chlamydiaceae in small domestic ruminants from Central Karakoram, Pakistan
Infectious keratoconjunctivitis (IKC) is a contagious eye disease primarily caused byMycoplasma conjunctivaein domestic and wild Caprinae.Chlamydophilaspecies have also been detected in ruminants with IKC. The objectives of this study are to investigate the ocular infection ofM. conjunctivaeand Chlamydiaceae and assess its interaction in relation to IKC in sheep and goats from remote communities around the Central Karakoram National Park in Pakistan, performing a combination of cross-sectional and case–control study design. Mostly asymptomatic and endemic infections ofM. conjunctivaeand Chlamydiaceae were found in sheep (19.3 per cent and 4.5 per cent, respectively) and goats (9.5 per cent and 1.9 per cent, respectively) from all communities, assessed by qPCR. Prevalence significantly differed between species only forM. conjunctivae(P=0.0184), which was also more prevalent in younger sheep (P<0.01).Chlamydophila pecorumwas identified by sequencing and was related with IKC only when coinfection withM. conjunctivaeoccurred, which suggest a synergic interaction. Cluster analysis ofM. conjunctivaestrains revealed higher diversity of strains than expected, evidenced interspecific transmission and suggested a higher local livestock trade than previously assumed. These results highlight the widespread occurrence ofM conjunctivaein sheep worldwide and its implications for wildlife should be assessed from a conservation perspective.