scholarly journals Serum melatonin in dogs with congenital portosystemic shunting, with and without hepatic encephalopathy

2020 ◽  
Vol 187 (3) ◽  
pp. e23-e23
Author(s):  
Marisa da Fonseca Ferreira ◽  
Richard John Mellanby ◽  
Adam George Gow
Keyword(s):  
Hepatic Encephalopathy ◽  
Medical Records ◽  
Clinical Signs ◽  
Rank Correlation ◽  
Final Diagnosis ◽  
Neurological Dysfunction ◽  
Spearman Rank Correlation ◽  
Serum Samples ◽  
Portosystemic Shunting ◽  
Healthy Dogs

BackgroundMelatonin is a hormone produced and secreted primarily by the pineal gland and mainly metabolised in the liver. Increased melatonin concentrations have been reported in human cirrhosis and hepatic encephalopathy (HE), a syndrome of neurological dysfunction. The pathogenesis of canine HE is incompletely understood. Melatonin has been hypothesised as a contributor to the development of HE. The aim of this study was to investigate whether serum melatonin concentrations are increased in canine congenital portosystemic shunting (cPSS), with and without HE.MethodsMedical records were retrospectively reviewed, for which archived (−80°C) serum samples were available. A canine competitive ELISA was used to measure melatonin in two cohorts: dogs with a final diagnosis of cPSS (n=23) with and without clinical signs of HE, and healthy dogs (n=15).ResultsMelatonin concentrations were not significantly different (P=0.81) between healthy controls (median 27.2 pg/mL, range 19.8–161.5 pg/mL) and dogs with cPSS (median 25.7 pg/mL, range 18.5–244.9 pg/mL). Serum melatonin did not differ between cPSS patients with and without clinical signs of HE (P>0.99). No correlation was found between serum melatonin and blood ammonia (Spearman rank correlation coefficient, rs=−0.41, P=0.08).ConclusionSerum melatonin is not increased in canine cPSS with and without HE. We found no evidence that altered melatonin metabolism plays a role in the pathogenesis of cPSS-associated HE.

scholarly journals FRI0285 FILGOTINIB TREATMENT RESULTS IN REDUCTION OF BIOMARKERS ASSOCIATED WITH DISEASE IN PATIENTS WITH ANKYLOSING SPONDYLITIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 730.2-731
Author(s):  
W. P. Maksymowych ◽  
Y. Tian ◽  
O. K. Yoon ◽  
W. Barchuk ◽  
R. Galien ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
Disease Activity ◽  
Systemic Inflammation ◽  
Clustering Analysis ◽  
Rank Correlation ◽  
Spearman Rank Correlation ◽  
Serum Samples ◽  
Spearman Rank ◽  
Time Points ◽  
Meso Scale

Background:Ankylosing spondylitis (AS) is a chronic, immune-mediated disease characterized by inflammation of the sacroiliac joints and spine, and a young age of onset of 20–40 years. In the recent TORTUGA study, filgotinib (FIL), an oral, selective Janus kinase 1 (JAK1) inhibitor, significantly reduced AS disease activity compared with placebo (PBO).1Selective JAK1 inhibition by FIL has the potential to simultaneously block multiple inflammatory pathways, thus we analyzed biomarker concentrations in serum samples from TORTUGA.Objectives:To evaluate the impact of selective JAK1 inhibition with FIL on circulating disease associated biomarkers in adult patients with active AS enrolled in the TORTUGA study.Methods:TORTUGA (Clinicaltrials.gov identifierNCT03117270) was a 12-week, randomized, double-blind, placebo-controlled, phase 2 study. Patients were randomized 1:1 to FIL 200 mg (n=58) or PBO (n=58) once-daily. Serum samples (FIL n=56, PBO n=53) were collected at baseline (BL) and weeks 1, 4 and 12, and analyzed using the Meso Scale Discovery immunoassay platform (Meso Scale Diagnostics, Rockville, MD, USA) to evaluate 135 biomarkers. Biomarker concentration changes from BL were analyzed on paired patient data and reported for weeks 1, 4 and 12, and clustering analysis was performed. Correlation between the 135 biomarkers and selected clinical scores at BL was assessed by Spearman rank correlation analysis.Results:FIL treatment produced significant reductions in serum concentrations of multiple biomarkers associated with AS disease activity. Five clusters of biomarker response were identified based on the kinetics and magnitude of percent changes from BL. These clusters also represented discrete biological functions: cluster 1 (rapid, strong >50% decrease in all three time points) included systemic inflammation biomarkers eg, CRP, SAA; cluster 2 (>20% decrease in at least one time point) included immune cell biomarkers eg, MIP3B, IL12p40; cluster 3 (<20% decrease in all three time points) included cellular adhesion biomarkers eg, ICAM-1, VCAM-1; cluster 4 (delayed decrease) included matrix remodelling biomarkers eg, MMP1, TIMP1; and cluster 5 included biomarkers that exhibited a gradual increase in serum concentration with FIL treatment.Spearman rank correlation analyses showed that at BL, the systemic inflammation biomarkers CRP and SAA, as well as a number of biomarkers including ICAM-1 and MMP3, were positively correlated with BL AS disease activity score (ASDAS); conversely, only a few biomarkers showed a negative correlation with BL ASDAS, the cytokine receptor FLT3 and the chemotactic cytokine fractalkine (FRACTAL).Conclusion:In patients with active AS, FIL treatment significantly decreased levels of circulating biomarkers associated with active AS disease, including proinflammatory cytokines and chemokines, cell adhesion molecules, and markers of matrix remodelling. Clustering analysis revealed early and late biomarker changes associated with disease. These data are consistent with reduced AS disease activity in TORTUGA and suggest that FIL treatment leads to a rapid and sustained reduction of inflammation in AS.References:[1]van der Heijde Det al. Lancet2018;392:2378–87Acknowledgments:This study was funded by Gilead Sciences, Inc. Editorial support was provided by Fishawack Communications Inc and funded by Gilead Sciences, Inc.Disclosure of Interests:Walter P. Maksymowych Grant/research support from: AbbVie, Novartis, Pfizer, and UCB, Consultant of: AbbVie, Boehringer Ingelheim, Celgene, Eli Lilly, Galapagos, Janssen, Novartis, Pfizer, and UCB, Employee of: Chief Medical Officer of CARE Arthritis Limited, Speakers bureau: AbbVie, Janssen, Novartis, Pfizer, and UCB, Yuan Tian Employee of: Gilead Sciences Inc., Oh Kyu Yoon Shareholder of: Gilead Sciences Inc., Employee of: Gilead Sciences Inc., William Barchuk Shareholder of: Gilead Sciences Inc and Eli Lilly, Employee of: Current employee of Gilead Sciences Inc and a former employee of AbbVie, Eli Lilly, and Johnson & Johnson, René Galien Shareholder of: Galapagos, Employee of: Galapagos, Robin Besuyen Shareholder of: Galapagos, Employee of: Galapagos, Yihua Liu Shareholder of: Gilead Sciences Inc., Employee of: Gilead Sciences Inc., Amer M. Mirza Shareholder of: Gilead Sciences Inc., Employee of: Gilead Sciences Inc., Vlad Malkov Shareholder of: Gilead Sciences Inc., Employee of: Gilead Sciences Inc., Angie Hertz Shareholder of: Gilead Sciences Inc, Employee of: Gilead Sciences Inc

Retrospective Diagnosis of Pontocerebellar Hypoplasia Type 1B in a Family with Two Deceased Newborn Children

2020 ◽  
Author(s):  
Irena Bradinova ◽  
Silvia Andonova ◽  
Alexey Savov
Keyword(s):  
Clinical Signs ◽  
Final Diagnosis ◽  
Population Based ◽  
Neuromuscular Disorder ◽  
Population Characteristics ◽  
High Risk Population ◽  
Pontocerebellar Hypoplasia ◽  
Clinical Genetic ◽  
Retrospective Diagnosis ◽  
Newborn Children

AbstractPontocerebellar hypoplasia type 1B is a severe autosomal recessive neurologic disorder characterized by a combination of cerebellar and spinal motor neuron degeneration beginning at birth. Pontocerebellar hypoplasia type 1B is caused by mutations in EXOSC3 gene. High prevalence of the p.Gly31Ala mutation was found recently, especially in the Roma ethnic minority. We present a young Bulgarian Roma family with two deceased newborn children manifesting severe neuromuscular disorder including severe muscle weakness, respiratory distress, and multiple joint contractures. Based on the clinical signs and family's population characteristics, DNA testing for the previously described EXOSC3 in Bulgarian Roma mutation c.92G > C; p.Gly31Ala was performed on blood samples of both parents and they were found to be heterozygous carriers. This finding indirectly confirmed the diagnosis of pontocerebellar hypoplasia type B in the deceased offspring. Knowledge of population-specific molecular bases of genetic conditions was the key to final diagnosis in the presented family. Designing of population-based clinical-genetic panels may be a powerful diagnostic tool for patients with such origin. Preconception carrier screening in high-risk population groups is a feasible option to discuss.

HUBUNGAN BEBAN KERJA PERAWAT DENGAN KELENGKAPAN PENGISIAN DOKUMENTASI ASUHAN KEPERAWATAN

2020 ◽  
Vol 5 (2) ◽  
pp. 588
Author(s):  
Etlida Wati ◽  
Ulva Arini
Keyword(s):  
Nursing Care ◽  
Rank Correlation ◽  
Bivariate Analysis ◽  
Spearman Rank Correlation ◽  
Cross Sectional ◽  
Weight Category ◽  
Spearman Rank ◽  
Hospital Room ◽  
Analysis Technique ◽  
Nurse Workload

<p>Documentation is an activity of recording, reporting or recording an event and activities carried out in the form of providing services that are considered important and valuable. One factor that can influence documentation is the nurse's workload. The purpose of this study is to identify the relationship between nurses' workload and the application of documentation in the Hj. Anna Lasmanah Banjarnegara. This  research is quantitative with a cross sectional approach descriptive correlation design. Samples were taken with a total sampling of 65 nurses. Instruments to measure documentation using observation sheets. While the nurse workload instrument uses a questionnaire sheet. The analysis technique uses Spearman Rank correlation. Based on the research results of the workload of a nurse in the hospital room , most of them are in the weight category, as many as 46 respondents (70.8%). Application of nursing care documentation in the hospital room Hj. Anna Lasmanah Banjarnegara, most of them are respondents in the incomplete category as many as 63 respondents (96.9%). There is a significant relationship between nurse workload with the application of documentation, this is evidenced by the results of the Spearman Rank correlation bivariate analysis, which is r = 0.688 with p = 0.000 &lt;0.05. It is hoped that management will motivate nurses to complete the documentation of nursing care</p>

Efektivitas Komunikasi Pendamping Corporate Social Responsibility (CSR)

2020 ◽  
Vol 4 (6) ◽  
pp. 894
Author(s):  
Yara Falmira Dianira
Keyword(s):  
Statistical Tests ◽  
Rank Correlation ◽  
Communication Effectiveness ◽  
Message Content ◽  
Sources Of Information ◽  
Source Information ◽  
Spearman Rank Correlation ◽  
Level Of Understanding ◽  
Corporate Social

ABSTRACT An important factor for the success of a CSR program is effective communication. Communication will be effective if it has an impact. If the information is conveyed based on the needs, then the communication will be effective. This study aims to analyze the factors which are related to the effectiveness of CSR communication. This study used a census method to approach 37 participants who received CSR programs. The Data analysis used the Spearman rank correlation for the statistical tests. The results showed that there was a correlation between factors that have the strength of CSR companion communication (level of attractiveness of the companion, quality of message content, and sources of information) which have real communication at the level of understanding of the participants of the Kertajaya Creative Destination (KCD) CSR program. In addition, there is a real correlation the factors that have the strength of CSR companion communication (the level of credibility of the companion, the source information, and the level of the recipient) and having communication at the level of attitudes of participants in the Kertajaya Creative Destination (KCD) CSR program. However, there is no real correlation between CSR companion communication factors and participant actions.Keywords :communication effectiveness, CSR, elements of communication. ABSTRAK Faktor penting dari keberhasilan program CSR adalah komunikasi yang efektif. Komunikasi dikatakan efektif jika menimbulkan dampak. Bila informasi tersampaikan sesuai dengan kebutuhan, maka komunikasi yang dijalankan efektif. Penelitian ini bertujuan untuk menganalisis efektivitas komunikasi pendamping CSR. Penelitian ini menggunakan pendekatan sensus terhadap 37 orang peserta penerima program CSR. Analisis data menggunakan uji statistik korelasi rank Spearman. Hasil penelitian menunjukkan bahwa terdapat hubungan nyata antara faktor efektivitas komunikasi pendamping CSR (derajat daya tarik pendamping, kualaitas isi pesan, dan sumber informasi)  dengan efektivitas komunikasi pada tingkat pemahaman peserta program CSR Kertajaya Creative Destination (KCD). Selain itu, terdapat hubungan nyata antara faktor efektivitas komunikasi pendamping CSR (tingkat kredibilitas pendamping, sumber informasi, dan tingkat penerima) dengan efektivitas komunikasi pada tingkat sikap peserta program CSR Kertajaya Creative Destination (KCD). Namun, tidak terdapat hubungan nyata antara faktor efektivitas komunikasi pendamping CSR dengan tindakan peserta. Kata Kunci : CSR, efektivitas komunikasi, unsur-unsur komunikasi.

scholarly journals Normalisation of the psychometric encephalopathy score within the Cameroonian population

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Larissa Pessidjo Djomatcho ◽  
Mathurin Pierre Kowo ◽  
Antonin Ndjitoyap Ndam ◽  
Sylvain Raoul Simeni Njonnou ◽  
Gabin Ulrich Kenfack ◽  
...  
Keyword(s):  
Hepatic Encephalopathy ◽  
Healthy Volunteers ◽  
Clinical Signs ◽  
Minimal Hepatic Encephalopathy ◽  
Education Level ◽  
Multicentric Study ◽  
Tracing Test ◽  
Cirrhotic Patients ◽  
The Mean ◽  
Number Connection Test

Abstract Background Minimal hepatic encephalopathy (MHE) is the presence of neuropsychological abnormalities detectable by psychometric tests. Psychometric Hepatic Encephalopathy Score (PHES) is a gold standard test for the early diagnosis of MHE in cirrhotic patients. The aim of this study was to standardize the PHES in a healthy Cameroonian population and to evaluate the prevalence of MHE among cirrhotic patients. Methods This was a prospective, multicentric study from 1 December 2018 to 31 July 2019 in two groups: healthy volunteers and cirrhotic patients without clinical signs of hepatic encephalopathy. The results of the number connection test-A, number connection test-B, serial dotting test, line tracing test were expressed in seconds and those of the digit symbol test in points. Results A total of 102 healthy volunteers (54 men, 48 women) and 50 cirrhotic patients (29 men, 31 women) were included. The mean age was 38.1 ± 12.55 years in healthy volunteers and 49.3 ± 15.6 years in cirrhotic patients. The mean years of education level was 11.63 ± 4.20 years in healthy volunteers and 9.62 ± 3.9 years in cirrhotic patients. The PHES of the healthy volunteer group was − 0.08 ± 1.28 and the cut-off between normal and pathological values was set at − 3 points. PHES of the cirrhotic patients was − 7.66 ± 5.62 points and significantly lower than that of volunteers (p < 0.001). Prevalence of MHE was 74% among cirrhotic patients. Age and education level were associated with MHE. Conclusion PHES cut-off value in Cameroonians is − 3, with MHE prevalence of 74% among cirrhotic patients.

scholarly journals Does continuity in nursing staff matter? A pilot study on correlation of central line-associated bloodstream infections and employee turnover

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Thomas Scheier ◽  
Stefan P. Kuster ◽  
Mesida Dunic ◽  
Christian Falk ◽  
Hugo Sax ◽  
...  
Keyword(s):  
Nursing Staff ◽  
Employee Turnover ◽  
Rank Correlation ◽  
Bloodstream Infections ◽  
Central Line ◽  
Incidence Rates ◽  
Nursing Personnel ◽  
Care Hospital ◽  
Spearman Rank Correlation ◽  
Spearman Rank

Abstract Background Understaffing has been previously reported as a risk factor for central line-associated bloodstream infections (CLABSI). No previous study addressed the question whether fluctuations in staffing have an impact on CLABSI incidence. We analyzed prospectively collected CLABSI surveillance data and data on employee turnover of health care workers (HCW) to address this research question. Methods In January 2016, a semiautomatic surveillance system for CLABSI was implemented at the University Hospital Zurich, a 940 bed tertiary care hospital in Switzerland. Monthly incidence rates (CLABSI/1000 catheter days) were calculated and correlations with human resources management-derived data on employee turnover of HCWs (defined as number of leaving HCWs per month divided by the number of employed HCWs) investigated. Results Over a period of 24 months, we detected on the hospital level a positive correlation of CLABSI incidence rates and turnover of nursing personnel (Spearman rank correlation, r = 0.467, P = 0.022). In more detailed analyses on the professional training of nursing personnel, a correlation of CLABSI incidence rates and licensed practical nurses (Spearman rank correlation, r = 0.26, P = 0.038) or registered nurses (r = 0.471, P = 0.021) was found. Physician turnover did not correlate with CLABSI incidence (Spearman rank correlation, r =  −0.058, P = 0.787). Conclusions Prospectively determined CLABSI incidence correlated positively with the degree of turnover of nurses overall and nurses with advanced training, but not with the turnover of physicians. Efforts to maintain continuity in nursing staff might be helpful for sustained reduction in CLABSI rates.

scholarly journals FRI0177 MULTI-MODALITY IMAGING TO EVALUATE LUPUS NEPHRITIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 672.2-673
Author(s):  
C. Dykas ◽  
B. H. Rovin ◽  
M. Boesen ◽  
O. Kubassova ◽  
P. Lipsky
Keyword(s):  
Image Analysis ◽  
Renal Function ◽  
Rank Correlation ◽  
Renal Perfusion ◽  
Imaging Data ◽  
Spearman Rank Correlation ◽  
Dce Mri ◽  
Analysis Group ◽  
Multi Center Study ◽  
Mean Time

Background:Lupus nephritis (LN) remains a significant cause of morbidity and mortality in subjects with Systemic Lupus Erythematosus (SLE). The gold standard for evaluation of LN remains the kidney biopsy, whereas renal function is usually evaluated by eGFR and urinary protein:creatinine ratio. More effective and sensitive methodology is needed to assess LN and also the response to treatment. Functional imaging of the kidney using quantitative techniques has great potential, as it can assess kidney function and pathologic changes non-invasively by evaluating perfusion, oxygenation, cellular density and fibrosis.Objectives:The objective of this study was to develop a multi-modality imaging approach for the evaluation of the spectrum of pathologic changes in LN and to determine when imaging data correlated with renal functionMethods:In this multi-center study (NCT03180021), subjects who were having a standard of care renal biopsy for LN were asked to participate in the imaging evaluation. Local Institutional Review Board approval was obtained, and subjects signed an Informed Consent Form. Dynamic contrast enhanced MRI (DCE-MRI) was employed to detect changes in vascularization and perfusion, Diffusion Weighted Imaging (DWI) to assess interstitial diffusion, T2*Map/BOLD to evaluate tissue oxygenation and T1rho to evaluate fibrosis (Figure 1). Regions of interest were identified in the imaged kidneys and imaging parameters were correlated with measures of renal function, including eGFR and urinary protein: creatinine ratio. In DCE-MRI, we specifically focused on mean Maximum Enhancement (ME), mean Time to Peak Enhancement (TTP) and mean Time of Washout (Twashout) as indicators of renal perfusion.Results:Nine subjects have been evaluated to date and their imaging data assessed for quality. Evaluation of mean data from DCE-MRI has shown a significant correlation between renal perfusion and renal function. For example, as shown in the figure, the 24 hour protein concentration negatively correlated with ME (rs=-0.81, p=0.015), TTP (rs=-0.83, p=0.01) and Twashout (rs=-0.81.p=0.01, Spearman rank correlation). In addition, the protein:creatinine ratio also negatively correlated with ME (rs=-0.79, p=0.02), TTP (rs=-0.74, p=0.04) and Twashout (rs=-0.79, p=0.02, Spearman rank correlation).Conclusion:These initial results have established the feasibility of multi-modality imaging as a tool to evaluate LN in a multi-center study. Moreover, changes in perfusion detected by DCE-MRI significantly correlate with proteinuria and urinary protein:creatinine ratio. These results suggest that multiparameter imaging may contribute useful data in the evaluation of subjects with LN.Figure:Disclosure of Interests:Claire Dykas: None declared, Brad H Rovin Grant/research support from: GSK, Consultant of: GSK, Mikael Boesen Consultant of: AbbVie, AstraZeneca, Eli Lilly, Esaote, Glenmark, Novartis, Pfizer, UCB, Paid instructor for: IAG, Image Analysis Group, AbbVie, Eli Lilly, AstraZeneca, esaote, Glenmark, Novartis, Pfizer, UCB (scientific advisor)., Speakers bureau: Eli Lilly, Esaote, Novartis, Pfizer, UCB, Olga Kubassova Shareholder of: IAG, Image Analysis Group, Consultant of: Novartis, Takeda, Lilly, Employee of: IAG, Image Analysis Group, Peter Lipsky Consultant of: Horizon Therapeutics

scholarly journals In vivo calibration of the T2* cardiovascular magnetic resonance method at 1.5 T for estimation of cardiac iron in a minipig model of transfusional iron overload

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Peter Diedrich Jensen ◽  
Asbjørn Haaning Nielsen ◽  
Carsten Wiberg Simonsen ◽  
Ulrik Thorngren Baandrup ◽  
Svend Eggert Jensen ◽  
...  
Keyword(s):  
Rank Correlation ◽  
Calibration Data ◽  
Iron Loading ◽  
Spearman Rank Correlation ◽  
Calibration Equation ◽  
Spearman Rank ◽  
Cardiac Iron ◽  
In Vivo Calibration ◽  
Spearman Rank Correlation Coefficient

Abstract Background Non-invasive estimation of the cardiac iron concentration (CIC) by T2* cardiovascular magnetic resonance (CMR) has been validated repeatedly and is in widespread clinical use. However, calibration data are limited, and mostly from post-mortem studies. In the present study, we performed an in vivo calibration in a dextran-iron loaded minipig model. Methods R2* (= 1/T2*) was assessed in vivo by 1.5 T CMR in the cardiac septum. Chemical CIC was assessed by inductively coupled plasma-optical emission spectroscopy in endomyocardial catheter biopsies (EMBs) from cardiac septum taken during follow up of 11 minipigs on dextran-iron loading, and also in full-wall biopsies from cardiac septum, taken post-mortem in another 16  minipigs, after completed iron loading. Results A strong correlation could be demonstrated between chemical CIC in 55 EMBs and parallel cardiac T2* (Spearman rank correlation coefficient 0.72, P < 0.001). Regression analysis led to [CIC] = (R2* − 17.16)/41.12 for the calibration equation with CIC in mg/g dry weight and R2* in Hz. An even stronger correlation was found, when chemical CIC was measured by full-wall biopsies from cardiac septum, taken immediately after euthanasia, in connection with the last CMR session after finished iron loading (Spearman rank correlation coefficient 0.95 (P < 0.001). Regression analysis led to the calibration equation [CIC] = (R2* − 17.2)/31.8. Conclusions Calibration of cardiac T2* by EMBs is possible in the minipig model but is less accurate than by full-wall biopsies. Likely explanations are sampling error, variable content of non-iron containing tissue and smaller biopsies, when using catheter biopsies. The results further validate the CMR T2* technique for estimation of cardiac iron in conditions with iron overload and add to the limited calibration data published earlier.

scholarly journals Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA

1992 ◽  
Vol 34 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Eide Dias Camargo ◽  
Paulo Mutuko Nakamura ◽  
Adelaide José Vaz ◽  
Marcos Vinícius da Silva ◽  
Pedro Paulo Chieffi ◽  
...  
Keyword(s):  
Low Cost ◽  
Clinical Signs ◽  
Toxocara Canis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Normal Individuals ◽  
Before And After ◽  
Dot Elisa ◽  
Stability Time

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

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