High-quality and homogeneous Arabidopsis thaliana plants from a simple and inexpensive method of hydroponic cultivation

A methodology is presented for the hydroponic cultivation of Arabidopsis thaliana (L.) Heynh. that is simple and inexpensive to construct from easily obtainable and reusable materials. High-quality and uniform plant material, free of algae and disease, are consistently produced through all life-stages. Minor modifications to previous protocols that reduce cost and labour include the use of small food-storage containers with snap-on lids, aluminum foil wrap to cover the containers to occlude light, commercial hydroponic fertilizer as nutrient solution, and clipped 1.5 mL microfuge tubes as plug holders. Seed is germinated on nutrient agar plates, instead of directly on rockwool plugs; after the seedlings have grown to the six-leaf stage, they are removed from the plates and the roots are enclosed within rockwool plugs that have been cut longitudinally. Subsequent seedling growth is very uniform and the elimination of oversowing and subsequent thinning results in a dramatic reduction in seed and seedling wastage.

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Effect of N:K Ratio and Electrical Conductivity of Nutrient Solution on Growth and Yield of Hydroponically Grown Golden Thistle (Scolymus hispanicus L.)

Proceedings ◽

10.3390/proceedings2019030087 ◽

2020 ◽

Vol 30 (1) ◽

pp. 87

Author(s):

Dimitrios Papadimitriou ◽

Emmanouil Kontaxakis ◽

Ioannis Daliakopoulos ◽

Thrassyvoulos Manios ◽

Dimitrios Savvas

Keyword(s):

Electrical Conductivity ◽

Nutrient Solution ◽

Chlorophyll Concentration ◽

Tuberous Root ◽

Growth And Yield ◽

Nutrient Concentrations ◽

Fresh Weight ◽

Natural Habitats ◽

High Quality ◽

Hydroponic Cultivation

As the demand for high-quality wild greens rises, due to their high nutritional, culinary, and medicinal properties, the potential overexploitation and excessive disruption of their natural habitats bring serious environmental problems to the foreground. However, new alternative cultivation techniques, such as hydroponic cultivation, could take advantage of rational water management, optimal fertilization management and climate adaptation, to produce high-quality wild greens, all year round. As an initial step to assess optimal hydroponic cultivation conditions for golden thistle (Scolymus hispanicus L.), in this study we evaluated the effect of N:K ratio and electrical conductivity (EC) in the supplied nutrient solution on plant growth, yield and phenology. Four nutrient solutions were applied with a low or a high N:K ratio (1.59 or 2.38 mol/mol, respectively) combined with a low or a high electrical conductivity (EC) level (2.2 and 2.8 dS m−1, respectively) in a 2 × 2 factorial experiment set as a completely randomized block design with 4 blocks and 48 plants per block. Golden thistle seedlings were planted in plastic growth-bags of hydroponic perlite substrate in an open, drip-irrigated, soilless cultivation system. The experiment commenced in December 2018, in a plastic greenhouse at the campus of the Hellenic Mediterranean University, Crete, Greece. After four months of cultivation, the post-harvest analysis showed that the high N:K ratio significantly increased the fresh weight of leaf and edible tuberous root, whereas the tested EC levels in the nutrient solution had no impact on plant fresh weight. The experimental treatments did not significantly affect leaf chlorophyll concentration (SPAD meter readings), chlorophyll fluorescence (Fv/Fm) or the number of leaves and the specific weight of the tuberous root of the plants. Our results indicate that wild golden thistle could be domesticated as an edible vegetable, and cultivated hydroponically at different seasons of the year using relatively low nutrient concentrations, thereby minimizing aquifer nitrate and phosphate pollution. A nutrient solution with a relatively high N:K ratio (here 2.38 mol/mol) is recommended for the hydroponic cultivation of golden thistle.

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Effects of Node Position and Electric Conductivity of Nutrient Solution on Adventitious Rooting of Nasturtium (Tropaeolum majus L.) Cuttings

Agronomy ◽

10.3390/agronomy11020363 ◽

2021 ◽

Vol 11 (2) ◽

pp. 363

Author(s):

Wenshuo Xu ◽

Na Lu ◽

Masao Kikuchi ◽

Michiko Takagaki

Keyword(s):

Electric Conductivity ◽

Nutrient Solution ◽

Adventitious Rooting ◽

Cutting Propagation ◽

Apical Bud ◽

Hydroponic Cultivation ◽

Bud Position ◽

Seed Propagation ◽

Node Position

Nasturtium is a popular herbal plant, widely cultivated as culinary and medicinal plants all over the world. However, the seed propagation of nasturtium is inefficient, and in-vitro propagation is sophisticated and high-cost. In this study, the cutting propagation method was employed to produce nasturtium seedlings. We aimed to determine the optimal conditions for cutting propagation of nasturtium seedlings by investigating the effects of node position and electric conductivity (EC) of nutrient solution on the root formation of the cuttings. Cuttings from five node positions (apical bud, 2nd node, 3rd node, 4th node, and 5th node) were subjected to water and five EC (1.0, 2.0, 3.0, 4.0, and 5.0 dS m−1) treatments with a hydroponic cultivation system in a plant factory. Results showed that all cuttings rooted successfully within two weeks. The cuttings from the apical bud position rooted earliest and produced the most roots regardless of EC level. Cuttings from other node positions produced longer roots and heavier root fresh and dry weights than those from the apical bud position. The cuttings under EC of 1.0 dS m−1 had the greatest root number, the longest root length, and the heaviest root fresh and dry weights regardless of node positions. The EC of 1.0 dS m−1 is considered the best condition for nasturtium cuttings for the range of EC tested in this study, and the cuttings from all the five node positions can be used as seedling materials.

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Influence of cutting type and size on rooting of Lavandula dentata L

Revista Brasileira de Plantas Medicinais ◽

10.1590/s1516-05722012000100002 ◽

2012 ◽

Vol 14 (1) ◽

pp. 8-11 ◽

Cited By ~ 4

Author(s):

C.M. Bona ◽

I.R. Biasetto ◽

M. Masetto ◽

C. Deschamps ◽

L.A. Biasi

Keyword(s):

Essential Oil ◽

Vegetative Propagation ◽

Plant Material ◽

The Other ◽

Root Weight ◽

Root Number ◽

Polystyrene Foam ◽

High Quality ◽

Cutting Propagation ◽

Superior Clones

Even though the Lavandula species may be propagated by seeds, it should not be the preferred propagation method because it causes a great lack of uniformity. On the other hand, asexually propagated lavender crops would provide more homogeneous crops, and clones from high quality plant material would increase the odds for obtaining a higher quality essential oil. However, problems such as poor rooting and restrict market availability for superior clones have been a problem in vegetative propagation of the Lavandula species. The objective of this work was to define which type and size of cutting is more adequate for cutting propagation of L. dentata, a very productive Lavandula species. Cuttings with 5, 8, 10 or 13 cm and from the apical or basal parts of stems cut from L. dentata stock plants were placed in Plantmax HT® filled polystyrene foam trays and kept under intermittent mist system for two months. Averages of root number, length of the longest root, fresh and dry root weight, and percentage of rooting were evaluated. Apical cuttings combined 97.9% rooting with an average of 13.2 roots per cutting and basal cuttings 93.7% rooting with 2.98 roots per cutting. Apical cuttings with at least 10 cm in length were considered the most adequate for cutting propagation of L. dentata.

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Effect of NPK fertilization on production and leaf nutrient content of eucalyptus minicuttings in nutrient solution

Revista Brasileira de Ciência do Solo ◽

10.1590/s0100-06832011000100023 ◽

2011 ◽

Vol 35 (1) ◽

pp. 249-254

Author(s):

José Pereira Carvalho Neto ◽

Enilson de Barros Silva ◽

Reynaldo Campos Santana ◽

Paulo Henrique Grazziotti

Keyword(s):

Experimental Design ◽

Nutrient Solution ◽

Plant Material ◽

Management Practice ◽

Dry Weight ◽

Nutrient Content ◽

Fractional Factorial ◽

Nutrient Contents ◽

Adequate Nutrient ◽

Npk Fertilization

Adequate nutrient levels in plants vary according to the species or clone, age and management practice. Therefore, adjustments of the nutrient solution are often necessary according to the plant material for multiplication. This study aimed to evaluate the influence of NPK fertilization on production and leaf nutrient contents of eucalyptus cuttings in nutrient solution. The study was conducted from November 2008 to January 2009 in a greenhouse. The experimental design was completely randomized fractional factorial (4 x 4 x 4)½, with a total of 32 treatments with three replications. The treatments consisted of four doses of N (50, 100, 200 and 400 mg L-1) as urea, P (7.5, 15, 30 and 60 mg L-1) in the form of phosphoric acid and K (50, 100, 200 and 400 mg L-1) in the form of potassium chloride in the nutrient solution. Only the effect of N alone was significant for the number and dry weight of minicuttings per ministump, with a linear decreasing effect with increasing N levels. The highest number of cuttings was obtained at a dose of 50, 7.5 and 50 mg L-1 of N, P and K, respectively.

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A nondestructive, rapid, reliable and inexpensive method to sample, store and extract high-quality DNA from fish body mucus and buccal cells

Molecular Ecology Notes ◽

10.1111/j.1471-8286.2005.01142.x ◽

2006 ◽

Vol 6 (1) ◽

pp. 257-260 ◽

Cited By ~ 39

Author(s):

LUCENTINI LIVIA ◽

PALOMBA ANTONELLA ◽

LANCIONI HOVIRAG ◽

NATALI MAURO ◽

FAUSTO PANARA

Keyword(s):

Buccal Cells ◽

Inexpensive Method ◽

High Quality

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The effect of growing media and concentration of nutrient solution on growth, flowering and macroelement content of media and leaves of Tymophylla tenuiloba Small

Acta Agrobotanica ◽

10.5586/aa.2002.043 ◽

2013 ◽

Vol 55 (2) ◽

pp. 57-64

Author(s):

Joanna Nowak

Keyword(s):

Nutrient Solution ◽

Element Content ◽

Mineral Element ◽

Flow Conditions ◽

High Quality ◽

Growing Media ◽

Bedding Plants ◽

Salt Level ◽

Total Soluble Salt ◽

Mineral Element Content

Effects of growing media and concentration of nutrient solution on growth, flowering, evapotranspiration and macroelement content of media and leaves of Tymophylla tenuiloba were evaluated under ebb-and-flow conditions. Two media: peat and peat + perlite (3:l, v/v), and four concentrations of nutrient solution: 1.0, 1.5, 2.0, 2.5 mS cm-1 were applied. High quality plants were produced in both media and all concentration of nutrient solution. The lowest evapotranspiration was measured at the highest concentration of nutrient solution. N concentration of leaves was high in all treatments. Concentrations of K, Ca, and Mg decreased with increasing concentration of nutrient solution. Opposite was found for P. At the end of cultivation the lowest pH was measured in the upper layer of growing media. The highest total soluble salt level was measured in the upper layers. Upper layers accumulated more N-NO3, P, Ca, and Mg. Mineral element content of both media was high in all concentrations of nutrient solution. Low concentration of nutrient solution at 1.0 mS cm-1 is recommended, although -1Tymophylla tenuiloba-1 can be also cultivated at higher concentrations of nutrient solution up to 2.5mS cm-1, if placed on the same bench with other bedding plants requiring more nutrients.

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SEMI-HYDROPONIC CULTIVATION AS A SELECTION TOOL FOR ALUMINUM TOLERANCE IN FORAGE GRASSES

Revista Engenharia na Agricultura - REVENG ◽

10.13083/reveng.v24i4.654 ◽

2016 ◽

Vol 24 (4) ◽

pp. 350-356

Author(s):

Gustavo André Colombo ◽

Aurélio Vaz-de-Melo ◽

Gilberto Coutinho Machado Filho ◽

André Silva de Sousa

Keyword(s):

Nutrient Solution ◽

Aluminum Toxicity ◽

Aluminum Tolerance ◽

Dry Weight ◽

Panicum Maximum ◽

Forage Grasses ◽

Breeding Programs ◽

Brachiaria Humidicola ◽

Hydroponic Cultivation ◽

Constant Improvement

The constant improvement of selection methods is necessary in order to optimize efficiency of breeding programs for aluminum tolerance. Thus, the aim of this study was to establish a vase volume to the ideal semi-hydroponic cultivation of forage grasses subjected to stress by aluminum in nutrient solution. The experimental design completely randomized, with four replicates, each replicate consisting of one plant per vase. The treatments were arranged in a factorial design 4x5, being the first factor consists of four genotypes of forage grasses Brachiaria Humidicola; Brachiaria brizantha cv. Piatã; Panicum maximum cv. Massai and Panicum maximum cv. Mombaça) and the second consisting of five separate volumes plastics vases where seedlings were grown (0.2; 0.25; 0.3; 0.35 and 0.4 dm³). The grasses grown in a semi-hydroponics system irrigated with nutrient solution rich in aluminum (3 mg L-1). Was measured plant height, dry weight of aereal part and root length. The semi-hydroponic cultivation in aluminum increased nutrient solution is effective in differentiation of forage grasses genotypes in relation to aluminum tolerance. Pots volumes near 0.3 dm3 promote greater development for root and aerial part attributes in forage grasses grown in nutrient solution with aluminum toxicity.

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Nutrient and inorganic solute (Na+ and Cl-) content in green onion plants under hydroponic cultivation using brackish water

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044013320 ◽

2020 ◽

Vol 44 ◽

Author(s):

Carlos Donato da Silva Souza ◽

Geronimo Ferreira da Silva ◽

Sirleide Maria de Menezes ◽

José Edson Florentino de Morais ◽

José Amilton Santos Júnior ◽

...

Keyword(s):

Nutrient Solution ◽

Brackish Water ◽

Hydroponic System ◽

Brackish Waters ◽

Randomized Design ◽

Hydroponic Cultivation ◽

Salinity Levels ◽

Completely Randomized Design ◽

Harmful Effects ◽

Different Levels

ABSTRACT Cultivation using brackish waters can result in nutritional and metabolic imbalances in several plant species, consequently reducing the production of dry matter (DM) and accumulation of toxic ions (Na+ and/or Cl-) in plants. We evaluated the DM production, and nutrient and inorganic solute (Na+ and Cl-) content in green onion plants (cv. Todo Ano Evergreen - Nebuka) under different levels of nutrient solution salinity in combination with circulation frequencies of this solution. Two experiments were conducted in a hydroponic system, using a completely randomized design, in a 6 × 2 factorial scheme, with five replicates: six levels of nutrient solution salinity (1.5, 3.0, 4.5, 6.0, 7.5, and 9.0 dS m-1) and two solution circulation frequencies (twice and thrice a day). In Experiment I, the evapotranspired depth was replaced using brackish water that was used to prepare each of the salinity levels (used exclusively), whereas in Experiment II, brackish water was used only to prepare each of the salinity levels and public water was used (electrical conductivity [ECw] = 0.12 dS m-1) for replacement in all treatments. The increase in the nutrient solution salinity reduced the production of DM and accumulation of nutrients; the reductions were more pronounced when brackish waters were used exclusively (Experiment I). However, the circulation of solutions thrice a day resulted in the harmful effects of the salinity effect. Replacing the evapotranspirated blade with water supply (Experiment II) mitigated the deleterious effects of salinity. Moreover, three circulations of the nutrient solution daily resulted in lower accumulation of inorganic Na+ and Cl- solutes and increased accumulation of nutrients N, P, K+, Ca2+, Mg2+, and S in the culture.

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