Molecular modification of triacylglycerol accumulation by over-expression of DGAT1 to produce canola with increased seed oil content under field conditionsThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

Botany ◽  
2009 ◽  
Vol 87 (6) ◽  
pp. 533-543 ◽  
Author(s):  
David C. Taylor ◽  
Yan Zhang ◽  
Arvind Kumar ◽  
Tammy Francis ◽  
E. Michael Giblin ◽  
...  
Keyword(s):  
Field Trial ◽  
Oil Content ◽  
Seed Oil ◽  
National Research Council ◽  
Plant Biotechnology ◽  
Field Trials ◽  
Substantial Effect ◽  
Oilseed Crop ◽  
Seed Oil Content ◽  
Over Expression

The final step in the Kennedy pathway for seed oil synthesis is catalyzed by an acyl-CoA-dependent diacylglycerol acyltransferase, DGAT1 (EC. 2.3.1.20). We have cloned DGAT1 genes from both Arabidopsis thaliana (L.) Heynh ecotype Columbia and Brassica napus ‘Jet Neuf’ and over-expressed them in canola under the control of the seed-specific promoter, napin. DGAT1 from A. thaliana was inserted into B. napus ‘Quantum,’ whereas DGAT1 from B. napus was introduced into the B. napus double haploid breeding line DH12075. Both sets of transgenic plants exhibited increased seed oil content in both greenhouse and in field trial settings, ranging from 2.5% to 7% of dried mass on an absolute basis. The ‘Quantum’ transgenic lines were field-tested in plots at Watrous, Saskatchewan, in 2006 and 2007. Larger scale field trials of the DH12075 transgenics were carried out in 2007 at Ellerslie and Vegreville, Alberta. This is the first study wholly dedicated to DGAT1 over-expression and the resultant oil-content increases in transgenic canola under field conditions. Collectively, the field trial results strongly support the hypothesis that the level of DGAT1 activity during seed development in an oilseed crop can have a substantial effect on the flow of carbon into seed oil. Therefore, the over-expression of DGAT1 is a positive strategy for increasing oil content and cultivar performance in canola.

scholarly journals Identification of candidate genes controlling oil content by combination of genome-wide association and transcriptome analysis in the oilseed crop Brassica napus

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhongchun Xiao ◽  
Chao Zhang ◽  
Fang Tang ◽  
Bo Yang ◽  
Liyuan Zhang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Candidate Genes ◽  
Oil Content ◽  
Seed Oil ◽  
Significant Snps ◽  
Genome Wide Association ◽  
Oilseed Crop ◽  
Seed Oil Content ◽  
Genome Wide ◽  
A Genome

Abstract Background Increasing seed oil content is one of the most important targets for rapeseed (Brassica napus) breeding. However, genetic mechanisms of mature seed oil content in Brassica napus (B. napus) remain little known. To identify oil content-related genes, a genome-wide association study (GWAS) was performed using 588 accessions. Results High-throughput genome resequencing resulted in 385,692 high-quality single nucleotide polymorphism (SNPs) with a minor allele frequency (MAF) > 0.05. We identified 17 loci that were significantly associated with seed oil content, among which 12 SNPs were distributed on the A3 (11 loci) and A1 (one loci) chromosomes, and five novel significant SNPs on the C5 (one loci) and C7 (four loci) chromosomes, respectively. Subsequently, we characterized differentially expressed genes (DEGs) between the seeds and silique pericarps on main florescences and primary branches of extremely high- and low-oil content accessions (HO and LO). A total of 64 lipid metabolism-related DEGs were identified, 14 of which are involved in triacylglycerols (TAGs) biosynthesis and assembly. Additionally, we analyzed differences in transcription levels of key genes involved in de novo fatty acid biosynthesis in the plastid, TAGs assembly and lipid droplet packaging in the endoplasmic reticulum (ER) between high- and low-oil content B. napus accessions. Conclusions The combination of GWAS and transcriptome analyses revealed seven candidate genes located within the confidence intervals of significant SNPs. Current findings provide valuable information for facilitating marker-based breeding for higher seed oil content in B. napus.

Efficacy of Impact® to manage blackleg (Leptosphaeria maculans) in canola

2002 ◽  
Vol 53 (3) ◽  
pp. 311 ◽  
Author(s):  
Ravjit K. Khangura ◽  
Martin J. Barbetti
Keyword(s):  
Seed Yield ◽  
Oil Content ◽  
Seed Oil ◽  
Leptosphaeria Maculans ◽  
Field Trials ◽  
Seed Oil Content ◽  
Resistant Cultivars ◽  
Moderate Disease ◽  
Moderately Resistant ◽  
Different Levels

The efficacy of the fungicide Impact® (a.i. flutriafol at 250 g/L) was tested for control of blackleg (Leptosphaeria maculans), and for improved yield and oil content in canola (Brassica napus) cultivars with varying levels of blackleg resistance. Field trials were conducted in 1996 in Western Australia at 3 locations (Merredin, Wongan Hills, Mt Barker) in paddocks containing 1–4-year-old blackleg-infested residues. The fungicide (400 mL product/ha) was coated on a double superphosphate fertiliser and applied at seeding. Blackleg was substantially reduced and the seed yield improved following the application of Impact® in most treatments at all locations except Mt Barker, where the fungicide had no effect on reducing the blackleg severity. The percentage reduction in blackleg severity with Impact® ranged between 18 and 59% and 1 and 43% at Merredin and Wongan Hills, respectively, in cultivars with different levels of resistance and exposed to infected residues of various ages. Likewise, the application of Impact® increased the seed yield by 40–322, 186–357, and 71–426 kg/ha at Merredin, Wongan Hills, and Mt Barker, respectively, on residue of various ages. Seed oil content was also improved following the application of Impact® in most treatments at all locations. The improvement in seed yield when using Impact® was variable for different ages of the residue, and was greater under severe to moderate disease conditions caused by exposure to more recent residues than under the milder disease conditions resulting from older residues. In general, susceptible to moderately resistant cultivars showed greater improvement in yield than resistant cultivars. The rates of Impact® were further evaluated in paddocks containing 3-year-old residue in field trials at the same 3 locations during 1997. The fungicide was applied at 200, 400, and 800 mL product/ha. Although blackleg severity was substantially reduced following application of Impact® at 400 and 800 mL/ha compared with 0 and 200 mL/ha, yield was improved only in some cultivars and at some locations.

scholarly journals The Brassica Napus Fatty Acid Exporter FAX1-1 Contributes to Biological Yield, Seed Oil Production, and Oil Quality

2021 ◽  
Author(s):  
Zhongchun Xiao ◽  
Fang Tang ◽  
Liyuan Zhang ◽  
Shengting Li ◽  
Shufeng Wang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Seed Yield ◽  
Oil Content ◽  
Seed Oil ◽  
Oil Quality ◽  
Oil Production ◽  
Oilseed Crop ◽  
Seed Oil Content ◽  
Biological Yield

Abstract Background: In the oilseed crop Brassica napus (rapeseed), various metabolic processes influence seed oil production, oil quality, and biological yield. However, the role of plastid membrane proteins in these traits has not been explored. Results: Our genome-wide association study (GWAS) of 520 B. napus accessions identified the chloroplast membrane protein-localized FATTY ACID EXPORTER 1-1 (FAX1-1) as a candidate associated with biological yield. Seed transcript levels of BnaFAX1-1 were higher in a cultivar with high seed oil content relative to a low-oil cultivar. BnaFAX1-1 localized to the plastid envelope. When expressed in Arabidopsis thaliana, BnaFAX1-1 enhanced biological yield (total plant dry matter), seed yield and seed oil content per plant. Likewise, in the field, B. napus BnaFAX1-1 overexpression lines (BnaFAX1-1-OE) displayed significantly enhanced biological yield, seed yield, and seed oil content compared with the wild type. BnaFAX1-1 overexpression also up-regulated gibberellic acid 4 (GA4) biosynthesis, which may contribute to biological yield improvement. Furthermore, oleic acid (C18:1) significantly increased in BnaFAX1-1 overexpression seeds. Conclusion: Our results indicated that the putative fatty acid exporter BnaFAX1-1 simultaneously improved seed oil production, oil quality and biological yield in B. napus, providing new approaches for future molecular breeding.

scholarly journals The Brassica napus fatty acid exporter FAX1-1 contributes to biological yield, seed oil content, and oil quality

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Zhongchun Xiao ◽  
Fang Tang ◽  
Liyuan Zhang ◽  
Shengting Li ◽  
Shufeng Wang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Seed Yield ◽  
Oil Content ◽  
Seed Oil ◽  
Genome Wide Association Study ◽  
Oil Quality ◽  
Oilseed Crop ◽  
Seed Oil Content ◽  
Biological Yield

Abstract Background In the oilseed crop Brassica napus (rapeseed), various metabolic processes influence seed oil content, oil quality, and biological yield. However, the role of plastid membrane proteins in these traits has not been explored. Results Our genome-wide association study (GWAS) of 520 B. napus accessions identified the chloroplast membrane protein-localized FATTY ACID EXPORTER 1-1 (FAX1-1) as a candidate associated with biological yield. Seed transcript levels of BnaFAX1-1 were higher in a cultivar with high seed oil content relative to a low-oil cultivar. BnaFAX1-1 was localized to the plastid envelope. When expressed in Arabidopsis thaliana, BnaFAX1-1 enhanced biological yield (total plant dry matter), seed yield and seed oil content per plant. Likewise, in the field, B. napus BnaFAX1-1 overexpression lines (BnaFAX1-1-OE) displayed significantly enhanced biological yield, seed yield, and seed oil content compared with the wild type. BnaFAX1-1 overexpression also up-regulated gibberellic acid 4 (GA4) biosynthesis, which may contribute to biological yield improvement. Furthermore, oleic acid (C18:1) significantly increased in BnaFAX1-1 overexpression seeds. Conclusion Our results indicated that the putative fatty acid exporter BnaFAX1-1 may simultaneously improve seed oil content, oil quality and biological yield in B. napus, providing new approaches for future molecular breeding.

scholarly journals Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight

2001 ◽  
Vol 126 (2) ◽  
pp. 861-874 ◽  
Author(s):  
Colette Jako ◽  
Arvind Kumar ◽  
Yangdou Wei ◽  
Jitao Zou ◽  
Dennis L. Barton ◽  
...  
Keyword(s):  
Seed Weight ◽  
Oil Content ◽  
Seed Oil ◽  
Diacylglycerol Acyltransferase ◽  
Seed Oil Content ◽  
Over Expression

Registration of ‘NC-Miller’ Soybean with High Yield and High Seed-Oil Content

2012 ◽  
Vol 6 (3) ◽  
pp. 294-297 ◽  
Author(s):  
J. W. Burton ◽  
L. M. Miranda ◽  
T. E. Carter ◽  
D. T. Bowman
Keyword(s):  
Oil Content ◽  
Seed Oil ◽  
High Yield ◽  
Seed Oil Content

scholarly journals Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in Brassica napus L.

2021 ◽  
Vol 22 (3) ◽  
pp. 1033
Author(s):  
Abirami Rajavel ◽  
Selina Klees ◽  
Johanna-Sophie Schlüter ◽  
Hendrik Bertram ◽  
Kun Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Brassica Napus ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content ◽  
Complex Interplay ◽  
Brassica Napus L ◽  
Data Set ◽  
Developmental Switches

Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of Brassica napus L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies.

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