Pathway of movement of apoplastic fluorescent dye tracers through the endodermis at the site of secondary root formation in corn (Zea mays) and broad bean (Vicia faba)

1981 ◽  
Vol 59 (5) ◽  
pp. 618-625 ◽  
Author(s):  
Carol A. Peterson ◽  
Mary E. Emanuel ◽  
G. B. Humphreys
Keyword(s):  
Zea Mays ◽  
Root Formation ◽  
Broad Bean ◽  
Primary Root ◽  
Root Primordia ◽  
Secondary Root ◽  
Point Of Entry ◽  
Primary Roots ◽  
Apoplastic Pathway ◽  
Root Treatment

The apoplastic dyes disodium 4,4′-bis (2-sulfostyryl) biphenyl (Tinopal CBS), a fabric brightener which is bound by cellulose, and trisodium, 3-hydroxy-5,8,10-pyrene trisulfonate (PTS), which is not bound by the walls, were used to trace an apoplastic pathway into the vascular tissues of corn and broad bean roots. The endodermis prevented dye entry into the stele in most regions of roots. However, if the roots were killed prior to dye treatment both dyes entered the steles, indicating that in healthy roots the dyes were confined to the apoplast and were not toxic in the applied concentrations. The more mobile dye, PTS, appeared in the shoots of the plants following a root treatment, indicating that an apoplastic pathway into the stele does exist in the roots. Neither dye penetrated into the steles at the tips of the primary roots. The point of entry in both corn and broad bean was located along the margin of a secondary root which had recently emerged from the epidermis of the primary root. The dyes did not enter the steles of the primary roots during earlier stages of secondary root development, nor did they enter through the secondary root primordia themselves.

scholarly journals Impact of soil compaction heterogeneity and moisture on maize (Zea mays L.) root and shoot development

2008 ◽  
Vol 54 (No. 12) ◽  
pp. 509-519 ◽  
Author(s):  
B. Konôpka ◽  
L. Pagès ◽  
C. Doussan
Keyword(s):  
Zea Mays ◽  
Soil Moisture ◽  
Soil Compaction ◽  
Statistical Tests ◽  
Lateral Roots ◽  
Primary Root ◽  
Leaf Biomass ◽  
Fine Soil ◽  
Primary Roots ◽  
Primary And Lateral Roots

Soil compaction heterogeneity and water content are supposed to be decisive factors influencing plant growth. Our experiment focused on simulation of two soil moisture levels (0.16 and 0.19 g/g) plus two levels of clod proportion (30 and 60% volume) and their effects on root and leaf variables of maize (<I>Zea mays</I> L.). We studied number of primary and lateral roots as well as primary root length at the particular soil depths. Statistical tests showed that the decrease rate of the number of roots versus depth was significantly affected by the two studied factors (<I>P</I> < 0.01). Soil moisture and clod occurrence, interactively, affected leaf biomass (<I>P</I> = 0.02). Presence of clods modified root morphological features. Particularly, the diameter of primary roots in the clods was significantly higher than of those grown in fine soil (<I>P</I> < 0.01). For primary roots, which penetrated clods, branching density decreased considerably for the root segments located just after the clods (<I>P</I> = 0.01). Regarding their avoidance to clods and tortuosity, large differences were found between primary roots grown in the contrasting soil environments.

Root Growth, Secondary Root Formation and Root Gravitropism in Carotenoid-deficient Seedlings of Zea mays L.

1985 ◽  
Vol 55 (3) ◽  
pp. 387-394 ◽  
Author(s):  
YUK-KIU NG ◽  
RANDY MOORE
Keyword(s):  
Zea Mays ◽  
Root Growth ◽  
Root Formation ◽  
Zea Mays L ◽  
Secondary Root ◽  
Root Gravitropism

scholarly journals Evaluation of Metaxylem Vessel Histogenesis and the Occurrence of Vessel Collapse during Early Development in Primary Roots of Zea mays ssp. mexicana: A Result of Premature Programmed Cell Death?

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 374
Author(s):  
Susumu Saito ◽  
Teruo Niki ◽  
Daniel K. Gladish
Keyword(s):  
Cell Death ◽  
Zea Mays ◽  
Programmed Cell Death ◽  
Primary Root ◽  
Cell Types ◽  
Root Apical Meristem ◽  
Root Tips ◽  
Primary Roots ◽  
Transmission Electron ◽  
Vessel Elements

Root apical meristem histological organization in Zea mays has been carefully studied previously. Classical histology describes its system as having a “closed organization” and a development of xylem that conforms to predictable rules. Among the first cell types to begin differentiation are late-maturing metaxylem (LMX) vessels. As part of a larger study comparing domestic maize root development to a wild subspecies of Z. mays (teosinte), we encountered a metaxylem development abnormality in a small percentage of our specimens that begged further study, as it interrupted normal maturation of LMX. Primary root tips of young seedlings of Zea mays ssp. mexicana were fixed, embedded in appropriate resins, and sectioned for light and transmission electron microscopy. Longitudinal and serial transverse sections were analyzed using computer imaging to determine the position and timing of key xylem developmental events. We observed a severe abnormality of LMX development among 3.5% of the 227 mexicana seedlings we screened. All LMX vessel elements in these abnormal roots collapsed and probably became non-functional shortly after differentiation began. Cytoplasm and nucleoplasm in the abnormal LMX elements became condensed and subdivided into irregularly-shaped “macrovesicles” as their cell walls collapsed inward. We propose that these seedlings possibly suffered from a mutation that affected the timing of the programmed cell death (PCD) that is required to produce functional xylem vessels, such that autolysis of the cytoplasm was prematurely executed, i.e., prior to the development and lignification of secondary walls.

scholarly journals Knock-Down of Arabidopsis PLC5 Reduces Primary Root Growth and Secondary Root Formation While Overexpression Improves Drought Tolerance and Causes Stunted Root Hair Growth

2018 ◽  
Vol 59 (10) ◽  
pp. 2004-2019 ◽  
Author(s):  
Qianqian Zhang ◽  
Ringo van Wijk ◽  
Xavier Zarza ◽  
Muhammad Shahbaz ◽  
Max van Hooren ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Root Growth ◽  
Root Hair ◽  
Hair Growth ◽  
Root Formation ◽  
Primary Root ◽  
Secondary Root ◽  
Knock Down ◽  
Root Hair Growth ◽  
Primary Root Growth

scholarly journals Screening of exotic and indigenous apple rootstocks for different horticultural traits

2017 ◽  
Vol 14 (2) ◽  
pp. 117-125
Author(s):  
KK Srivastava ◽  
DB Singh ◽  
Dinesh Kumar ◽  
JI Mir
Keyword(s):  
Root Length ◽  
Primary Root ◽  
Apple Rootstock ◽  
Apple Rootstocks ◽  
Leaf Petiole ◽  
Secondary Root ◽  
Root Numbers ◽  
Horticultural Traits ◽  
Primary Roots ◽  
Secondary Roots

An experiment was carried out to find the suitable exotic as well as indigenous apple rootstocks for temperate zone of India. The rootstocks were screened on the basis of 11 important pomological traits. The data revealed that maximum numbers of primary and secondary roots were noted of 93.40, 92.76 in MM.106 and USA 106 and 92.0 noted in CITHrootstock- 01, respectively. Maximum length of primary roots (19.83cm) was recorded of EMLA 106 whereas shortest primary roots (6.47 cm) were recorded of ALNARP. The longest secondary roots (11.83cm) were found in M.27 and shortest secondary roots (2.10 cm) in ALNARP rootstock. Most of the indigenous apple rootstocks were having short to medium length of secondary roots. Leaf area was recorded highest (3.80cm2) in CITH-Apple rootstock-04 and smallest (1.96 cm2) of USA- 106. All the evaluated rootstocks have pink colored petiole except B-9, which has dark red color. Highest collar rot incidence was observed in M- 27 and M-26 and least in indigenous root stocks (CITH-Apple rootstocks). Highest wooly aphid infection was found in M-9, B-9, M-27, EMLA-106 and ALNARP whereas least infestation noted in M-26 and indigenous apple rootstock (CITH-ARS-01, CITH-ARS-02, CITH-ARS-03, CITH-ARS-04, CITH-ARS-, CITH-ARS-09 and CITH-ARS-10). Primary root length has positive correlation with secondary root numbers, secondary root length and leaf petiole length but negative correlation with leaf area.SAARC J. Agri., 14(2): 117-125 (2016)

scholarly journals Radioisotopic studies of DNA biosynthesis in relation to growth of Zea mays roots

2014 ◽  
Vol 55 (1) ◽  
pp. 97-106
Author(s):  
Roman Maksymowych ◽  
Andrew B. Maksymowych ◽  
Joseph A. Orkwiszewski
Keyword(s):  
Zea Mays ◽  
Cell Division ◽  
Dna Synthesis ◽  
Cell Elongation ◽  
Liquid Scintillation ◽  
Primary Root ◽  
Root Region ◽  
Dna Biosynthesis ◽  
Primary Roots ◽  
Meristematic Region

Root growth consists of two basic processes, cell division and cell elongation. An integral part of the first process is the synthesis of deoxyribonucleic acid (DNA). DNA biosynthesis was studied in primary roots of <em>Zea mays</em> through incorporation of <sup>3</sup>H-thymidine by autoradiography and liquid scintillation spectrometry. DNA synthesis was restricted to the meristematic region of the primary root. The curve representing this process was bell-shaped with a peak at 1.5 mm from the tip. Up to 3 mm distance from the tip, the root was growing both by cell division and cell elongation. This was also the region of DNA synthesis. The root region between 3 to 9 mm from the tip. was growing only by cell elongation. The relative elemental rate of elongation had a maximum at 3.5 mm from the tip, or shortly after cessation of DNA synthesis and cell division. Cells stopped elongating at 9 mm distance from the tip.

scholarly journals Effect of Preconditioning Treatments and Auxins on the Rooting of Semi-Hardwood Cuttings of Olive Planted During Winter Under Mist Condition

2016 ◽  
Vol 11 (2) ◽  
pp. 560-566
Author(s):  
Manish Thakur ◽  
D. D Sharma ◽  
Pramod Verma ◽  
Babita
Keyword(s):  
Leaf Area ◽  
Plant Height ◽  
Root Length ◽  
Primary Root ◽  
Rooted Cuttings ◽  
Secondary Root ◽  
Survival Percentage ◽  
Primary Roots ◽  
Primary Root Length ◽  
Secondary Roots

An experiment was conducted during winter to find the effect of girdling, etiolation and auxins i.e. IBA and NAA on rooting of semi- hardwood cuttings of olive cv. Leccino under mist. The experiment comprised of 13 treatments and was combinations of girdling, etiolation and auxins. The results indicated that the best rooting characteristics viz; highest percent rooted cuttings (53.33), number of primary roots (6.58) and secondary roots (8.53) and diameter (0.46 mm) were maximum with cuttings treated with IBA at 5000 ppm, where as the maximum primary root length (5.30 cm) and secondary root length (2.42) was recorded with the treatment girdling + IBA 4000 ppm + NAA 1000 ppm and IBA 4000 ppm, respectively. Regarding the shoot characteristics viz; plant height (14.59 cm) was recorded highest for the treatment IBA@ 4000 ppm which was at par with the treatment IBA @ 5000 ppm (14.26 cm). Highest plant diameter (2.40 mm), numbers of leaves (16.26) and leaf area (36.42 cm2) were highest for the treatment IBA @ 5000 ppm. Survival percentage of cuttings (71.57 %) was also highest in cuttings treated with IBA @ 5000 ppm. It is concluded that IBA at 5000 ppm was found to be the best treatment for propagation of olive through semi- hardwood cuttings during winter.

scholarly journals The Aux/IAA gene rum1 involved in seminal and lateral root formation controls vascular patterning in maize (Zea mays L.) primary roots

2014 ◽  
Vol 65 (17) ◽  
pp. 4919-4930 ◽  
Author(s):  
Yanxiang Zhang ◽  
Anja Paschold ◽  
Caroline Marcon ◽  
Sanzhen Liu ◽  
Huanhuan Tai ◽  
...  
Keyword(s):  
Zea Mays ◽  
Lateral Root ◽  
Root Formation ◽  
Zea Mays L ◽  
Lateral Root Formation ◽  
Vascular Patterning ◽  
Primary Roots

Morphometrics of the aerial roots of Kingia australis (Liliales)

1981 ◽  
Vol 29 (1) ◽  
pp. 81 ◽  
Author(s):  
B Lamont
Keyword(s):  
Fire History ◽  
Dead Zone ◽  
Dry Weight ◽  
Root Primordia ◽  
Stem Apex ◽  
Primary Roots ◽  
Aerial Roots ◽  
History Of ◽  
Lateral Branches ◽  
Potential Height

Kingia australis, common in the heaths and forests of south-western Australia, is distinguished from all other grass trees in Australia by the presence of a mantle of concealed aerial roots. A ring of up to 50 root primordia is initiated in winter from the stem apex. In plants more than 1 m high, initiation and commencement of elongation of the primary roots are no longer annual but dependent on the fire history of the plant. These roots descend between the stem and persistent leaf bases at about 2 cm per growing month, sending many lateral branches among the leaf bases. Aerial roots gradually replace the space occupied by the leaf bases until they may account for 45% of the dry weight of the aerial caudex. The caudex of one 6-m-high specimen bore up to 27 roots per cm2 transection of the root mantle, with about 3000 primary roots entering the soil. All underground primary roots (except the initial contractile roots) have an aerial origin and are concentrated vertically under the canopy. After 300-400 years the stem starts to die back from the base, and the aerial roots attached to that portion disintegrate. By propping up the stem and bridging the dead zone of the stem, the living aerial roots greatly extend the potential height and longevity of the plant. In addition, the hairy laterals are ideally located to absorb water and nutrients directly from the leaf bases. Protective and aerating functions are also indicated.

Early Pinus caribaea var. hondurensis root development. 2. Influence of soil strength

1996 ◽  
Vol 36 (7) ◽  
pp. 847 ◽  
Author(s):  
A Costantini ◽  
D Doley ◽  
HB So
Keyword(s):  
Root Development ◽  
Soil Type ◽  
Root Elongation ◽  
Primary Root ◽  
High Strength ◽  
Soil Strength ◽  
Shoot Development ◽  
Pinus Caribaea ◽  
Primary Shoot ◽  
Primary Roots

The influence of penetration resistance (PR), an easily measured indicator of soil strength, on the growth of Pinus caribaea var. hondurensis radicles and seedlings was investigated. Negative exponential relationships between PR and both radicle and primary root elongation were observed. All root elongation ceased at PR levels of 3.25 MPa. Tip diameters of radicles and primary roots were positively correlated with PR values up to 2.4 MPa, whilst numbers of primary roots, total root lengths and lengths of longest roots were all negatively correlated with PR. Hypocotyl elongation was also reduced by increasing PR, although the reductions occurred at higher PRs than those which inhibited root development. In contrast, primary shoot development was unaffected by PR levels which were sufficient to stop root elongation, but was reduced in soil with a PR of 4.8 MPa. There were significant family x soil type and family x PR interactions for radicle, hypocotyl, primary root and primary shoot development. 1f these interactions are correlated with performance in the field, then they may serve as useful indicators of family suitability to both soil type and high strength soils.

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