Alpha-amylase in seed hulls of developing Brassica campestris

1988 ◽  
Vol 66 (7) ◽  
pp. 1461-1464 ◽  
Author(s):  
L. Van Caeseele ◽  
A. W. MacGregor
Keyword(s):  
Starch Granule ◽  
Brassica Campestris ◽  
Cell Layer ◽  
Amylase Activity ◽  
Total Activity ◽  
Maximum Activity ◽  
Granule Size ◽  
Three Samples ◽  
Germinated Barley ◽  
Epidermal Cell Layer

Extracts of hulls from developing Brassica campestris and Brassica napus seeds were examined for α-amylase activity. The enzyme was compared with α-amylase from barley pericarp and germinated barley seeds, using isoelectric focusing. Enzymes of similar isoelectric point (pI) were present in B. campestris, B. napus, and barley pericarp. Additional bands of activity were present in B. napus. The major α-amylase component of germinated barley has a high pI, but no corresponding isozyme was detected in the other three samples. The increase in total activity of α-amylase in Brassica seed hulls coincided with the increase in starch granule size in the epidermal cell layer, with maximum activity approximately 16 days after pollination.

Starch Granule Size Distribution in Wheat Grains under Irrigated and Rainfed Conditions

2008 ◽  
Vol 34 (5) ◽  
pp. 795-802 ◽  
Author(s):  
Zhong-Min DAI
Keyword(s):  
Size Distribution ◽  
Starch Granule ◽  
Granule Size ◽  
Wheat Grains ◽  
Rainfed Conditions

scholarly journals Comparison of the GFFF and LALLS Methods for the Measurement of Starch Granule Size Distribution in Spring Barley Caryopses

2002 ◽  
Vol 108 (2) ◽  
pp. 200-203 ◽  
Author(s):  
V. Psota ◽  
I. Bohačenko ◽  
J. Hartmann ◽  
M. Budinská ◽  
J. Chmelík
Keyword(s):  
Size Distribution ◽  
Starch Granule ◽  
Spring Barley ◽  
Granule Size

Air classification of flours of three legume species: Effect of starch granule size distribution

1986 ◽  
Vol 37 (2) ◽  
pp. 173-184 ◽  
Author(s):  
Pauline Cloutt ◽  
Ann F. Walker ◽  
Derek J. Pike
Keyword(s):  
Size Distribution ◽  
Starch Granule ◽  
Granule Size ◽  
Legume Species ◽  
Air Classification ◽  
Species Effect

Carbohydrase activity in the pancreatic tissue and small intestine mucosa of sheep fed dried-grass or ground maize-based diets

1985 ◽  
Vol 104 (2) ◽  
pp. 435-443 ◽  
Author(s):  
A. N. Janes ◽  
T. E. C. Weekes ◽  
D. G. Armstrong
Keyword(s):  
Small Intestine ◽  
Amylase Activity ◽  
Total Activity ◽  
Pancreatic Tissue ◽  
Proximal Region ◽  
Small Intestinal Mucosa ◽  
Intestine Mucosa ◽  
Ruminant Animals ◽  
Specific Activities ◽  
Small Intestinal

SummaryTwo groups of six sheep were fed either dried-grass or ground maize-based diets for at least 4 weeks before slaughter. Samples of the small intestinal mucosa and spancreatic tissue were assayed for a-amylase, glucoamylase, maltase and oligo-l,6-glucosidase.The pancreatic tissue contained high activities of α-amylase and much lower activities of glucoamylase, maltase and oligo-1,6-glucosidase. There was no effect of diet on the specific activities of any of these enzymes in the pancreatic tissue.The activity of α-amylase adsorbed on to the mucosa of the small intestine was greatest in the proximal region of the small intestine, the activity generally declining with increasing distance away from the pylorus. There was no diet effect on the absorbed α-amylase activity.Similar patterns of distribution along the small intestine were observed for maltase, glucoamylase and oligo-1,6-glucosidase with the highest activities in t he jejunum. There was no overall effect of diet on glucoamylase or maltase specific activities and glucoamylase total activity, although the total activities of maltase and oligo-1,6-glucosidase were significantly greater for the sheep fed the ground maize-based diet (P < 0·05).It is suggested that ruminant animals may be capable of digesting large amounts of starch in the small intestine through an adaptation in the activity of the host carbohydrases.

Genome-wide association mapping of starch granule size distribution in common wheat

2017 ◽  
Vol 77 ◽  
pp. 211-218 ◽  
Author(s):  
Jieyun Li ◽  
Awais Rasheed ◽  
Qi Guo ◽  
Yan Dong ◽  
Jindong Liu ◽  
...  
Keyword(s):  
Association Mapping ◽  
Size Distribution ◽  
Common Wheat ◽  
Starch Granule ◽  
Granule Size ◽  
Genome Wide Association ◽  
Genome Wide

scholarly journals KARAKTERISTIK PATI BERPORI MIKRO DARI TAPIOKA HASIL PERLAKUAN AMILASE SEBAGAI AGEN PENJERAPAN MINYAK [The Characteristic of Microporous Tapioca Starch After Amylase Treatment For Oil Adsorbent Agent]

2020 ◽  
Vol 25 (2) ◽  
pp. 71
Author(s):  
Dwi Ajias Pramasari ◽  
Dewi Sondari ◽  
Danang Sudarwoko Adi ◽  
Bernadeta Ayu Widyaningrum ◽  
Anugerah Fajar ◽  
...  
Keyword(s):  
Oil Palm ◽  
Functional Group ◽  
Starch Granule ◽  
Granule Size ◽  
The Other ◽  
Partial Hydrolysis ◽  
Color Analysis ◽  
Tapioca Starch ◽  
Granule Surface ◽  
The One

Microporous starch can be used as oil adsorbent agent. The microporous starch can be produced through partial hydrolysis at temperature below gelatinization point using amylase. On the other hand, the study of amylase produced from Indonesian sea microbe, especially Brevibacterium sp. was rarely studied. Therefore, this paper discusses the tapioca characteristic made from Brevibacterium sp. amylase (treatment A) and commercial amylase (treatment B) as oil adsorbent agent. The result showed that the yield from treatment A and B was 74.65% and 12.75% while the starch granule size was 14.60 μm and 12,59 μm. The adsorbent test showed adsorption level of oil palm were 91,08% and 142,14% while for olive oil were 94,70% and 133,17%, for treatment A and B, respectively. The morphological test showed the presence of pori on the granule surface for both treatments with FTIR assessment showed no significant change in chemical functional group for both treatments. The color analysis showed almost similar brightness level between two treatments. In the end, microporous starch of treatment A has prospect as oil adsorbent agent like the one from commercial amylase

scholarly journals A comparative evaluation of Kumaun Himalayan Gymnosperms for their Antifungal potential against plant pathogenic fungi

2018 ◽  
Vol 7 (3) ◽  
pp. 230-241
Author(s):  
Savita Joshi ◽  
◽  
Parikshit Kumar ◽  
Prabha Pant ◽  
SC Sati ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Total Activity ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Cedrus Deodara ◽  
Araucaria Cunninghamii

Fungicidal activity of 10 ethnobotanically known Kumaun Himalayan gymnospermous plants namely Araucaria cunninghamii, Biota orientalis, Cedrus deodara, Cephalotaxus griffithi, Cryptomeria japonica Cupressus torulosa, Ginkgo biloba, Juniperus communis, Picea smithiana and Pinus wallichiana were tested against six plant disease causing fungal pathogens by agar well-diffusion method. Forty extracts of these gymnospermic leaves in different organic solvents (methanol, ethanol, chloroform and hexane) were studied by performing the 160 sets of experiments. The MIC values of each extract (where % inhibition ≥ 40%) were also determined. All the plant extracts exhibited strong antifungal activity. Results indicated that all leaves extracts of C. griffithi and G. biloba were found most effective among the tested plants extracts. Hexane extract of C. griffithi was showed highest inhibitory activity against C. falcatum (72%; MIC, 7.81µg/ml) and T. indica (70%; MIC, 15.62µg/ml). On the other hand, ethanol extract of G. biloba also showed remarkable activity against P. oryzae (66% with MIC, 7.81g/ml). While P. wallichiana leave extracts were found less active among the studied plants against all the tested fungal strains. The chloroform extracts were found the most effective against all the tested fungi (10% to 60%), followed by ethanol extract (30-50%), methanol extract (20-40%), while in hexane extracts ranged 10-30% only. The extracts of C. griffithi exhibited superior Relative Antifungal Activity (RAA, 20%), followed by G. biloba and A. cunninghamii (RAA, 19 and 12%, respectively). All data were also analyzed for determination of total activity of plant for each studied species of gymnosperm. C. griffithi had maximum activity i.e. 71 % followed by G. biloba (54%) and A. cunninghamii (33%). C. torulosa showed the least total activity and RAA i.e. 8% and 3%, respectively. All the plant species assayed possess definite antifungal properties and suggested for phytochemical analysis to identify the active principles responsible for their antifungal activity

scholarly journals Starch Granule Size and Morphology of Arabidopsis thaliana Starch-Related Mutants Analyzed during Diurnal Rhythm and Development

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5859
Author(s):  
Qingting Liu ◽  
Yuan Zhou ◽  
Joerg Fettke
Keyword(s):  
Arabidopsis Thaliana ◽  
Starch Granule ◽  
Granule Size ◽  
High Temporal Resolution ◽  
Starch Granules ◽  
Transitory Starch ◽  
Young Leaves ◽  
Size And Morphology ◽  
Safranin O

Transitory starch plays a central role in the life cycle of plants. Many aspects of this important metabolism remain unknown; however, starch granules provide insight into this persistent metabolic process. Therefore, monitoring alterations in starch granules with high temporal resolution provides one significant avenue to improve understanding. Here, a previously established method that combines LCSM and safranin-O staining for in vivo imaging of transitory starch granules in leaves of Arabidopsis thaliana was employed to demonstrate, for the first time, the alterations in starch granule size and morphology that occur both throughout the day and during leaf aging. Several starch-related mutants were included, which revealed differences among the generated granules. In ptst2 and sex1-8, the starch granules in old leaves were much larger than those in young leaves; however, the typical flattened discoid morphology was maintained. In ss4 and dpe2/phs1/ss4, the morphology of starch granules in young leaves was altered, with a more rounded shape observed. With leaf development, the starch granules became spherical exclusively in dpe2/phs1/ss4. Thus, the presented data provide new insights to contribute to the understanding of starch granule morphogenesis.

Analysis and Specification of Starch Granule Size Distributions

10.3791/61586 ◽  
2021 ◽  
Author(s):  
Ming Gao ◽  
Mahta Moussavi ◽  
Deland Myers
Keyword(s):  
Starch Granule ◽  
Granule Size ◽  
Size Distributions
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