The arrangement of microtubules in leaves of monocotyledonous and dicotyledonous plants

1989 ◽  
Vol 67 (12) ◽  
pp. 3506-3512 ◽  
Author(s):  
Taizo Hogetsu
Keyword(s):  
Cell Wall ◽  
Zea Mays ◽  
Avena Sativa ◽  
Epidermal Cells ◽  
Cellulose Microfibrils ◽  
Cortical Microtubules ◽  
Monocotyledonous Plant ◽  
Pisum Sativum L ◽  
Dicotyledonous Plants ◽  
Parenchymal Cells

The first leaf of Avena sativa L., a monocotyledonous plant, grows in a region that lies within 10 mm of the base of the leaf. Cells in that region elongate longitudinally but hardly expand laterally. The orientation of cortical microtubules in the elongating region is transverse in both epidermal and parenchymal cells. The same features of the arrangement of microtubules are also observed in the leaves of Zea mays. Cellulose microfibrils in the cell wall are coaligned with microtubules, lying approximately transverse to the axis of elongation, as if they function as hoops to facilitate the longitudinal elongation of the cell. The cells of growing leaves of Pisum sativum L., a dicotyledonous plant, expand superficially in every direction at every point on the leaf. Cortical microtubules lining the outer walls of epidermal cells are arranged randomly or in parallel. The parallel microtubules are oriented in various directions. In the outer walls of epidermal cells of growing leaves, areas with different predominant orientations of microfibrils are found within a single cell, consistent with the arrangement of microtubules. These results indicate that the orientation of cortical microtubules is correlated with the orientation of microfibrils and the direction of growth in growing leaves of both monocotyledons and dicotyledons, suggesting the involvement of cortical microtubules in control of the direction of growth in leaves.

scholarly journals THE STRUCTURE OF THE PRIMARY EPIDERMAL CELL WALL OF AVENA COLEOPTILES

1957 ◽  
Vol 3 (2) ◽  
pp. 171-182 ◽  
Author(s):  
S. T. Bayley ◽  
J. R. Colvin ◽  
F. P. Cooper ◽  
Cecily A. Martin-Smith
Keyword(s):  
Cell Wall ◽  
Outer Wall ◽  
Epidermal Cells ◽  
Cellulose Microfibrils ◽  
X Rays ◽  
Primary Wall ◽  
Normal Type ◽  
Number Of Layers ◽  
Angle Scattering ◽  
Epidermal Cell Wall

The primary walls of epidermal cells in Avena coleoptiles ranging in length from 2 to 40 mm. have been studied in the electron and polarizing microscopes and by the low-angle scattering of x-rays. The outer walls of these cells are composed of multiple layers of cellulose microfibrils oriented longitudinally; initially the number of layers is between 10 and 15 but this increases to about 25 in older tissue. Where epidermal cells touch, these multiple layers fuse gradually into a primary wall of the normal type between cells. In these radial walls, the microfibrils are oriented transversely. Possible mechanisms for the growth of the multilayered outer wall during cell elongation are discussed.

scholarly journals Mechanical properties of the stigmatic cell wall mediate pollen tube path in Arabidopsis

2018 ◽  
Author(s):  
Lucie Riglet ◽  
Frédérique Rozier ◽  
Chie Kodera ◽  
Isabelle Fobis-Loisy ◽  
Thierry Gaude
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Cell Shape ◽  
Cell Imaging ◽  
Pollen Tubes ◽  
Mechanical Anisotropy ◽  
Cellulose Microfibrils ◽  
Mechanical Forces ◽  
Cortical Microtubules ◽  
Isotropic Reorientation

ABSTRACTSuccessful fertilization in angiosperms depends on the proper trajectory of pollen tubes through the pistil tissues to reach the ovules. Pollen tubes first grow within the cell wall of the papilla cells, applying pressure to the cell. Mechanical forces are known to play a major role in plant cell shape by controlling the orientation of cortical microtubules (CMTs), which in turn mediate deposition of cellulose microfibrils (CMFs). Here, by combining cell imaging and genetic approaches, we show that isotropic reorientation of CMTs and CMFs in aged and katanin1-5 (ktn1-5) papilla cells is accompanied by a tendency of pollen tubes to coil around the papillae. Furthermore, we uncover that aged and ktn1-5 papilla cells have a softer cell wall and provide less resistance to pollen tube growth. Our results reveal an unexpected role for KTN1 in pollen tube guidance by ensuring mechanical anisotropy of the papilla cell wall.

scholarly journals KATANIN-dependent mechanical properties of the stigmatic cell wall mediate the pollen tube path in Arabidopsis

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Lucie Riglet ◽  
Frédérique Rozier ◽  
Chie Kodera ◽  
Simone Bovio ◽  
Julien Sechet ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Wall ◽  
Pollen Tube ◽  
Cell Walls ◽  
Cell Shape ◽  
Pollen Tubes ◽  
Mechanical Anisotropy ◽  
Cellulose Microfibrils ◽  
Cortical Microtubules ◽  
Isotropic Reorientation

Successful fertilization in angiosperms depends on the proper trajectory of pollen tubes through the pistil tissues to reach the ovules. Pollen tubes first grow within the cell wall of the papilla cells, applying pressure to the cell. Mechanical forces are known to play a major role in plant cell shape by controlling the orientation of cortical microtubules (CMTs), which in turn mediate deposition of cellulose microfibrils (CMFs). Here, by combining imaging, genetic and chemical approaches, we show that isotropic reorientation of CMTs and CMFs in aged Col-0 and katanin1-5 (ktn1-5) papilla cells is accompanied by a tendency of pollen tubes to coil around the papillae. We show that this coiled phenotype is associated with specific mechanical properties of the cell walls that provide less resistance to pollen tube growth. Our results reveal an unexpected role for KTN1 in pollen tube guidance on the stigma by ensuring mechanical anisotropy of the papilla cell wall.

scholarly journals Torsions-Driven Root Helical Growth, Waving And Skewing In Arabidopsis

2021 ◽  
Author(s):  
Ke Zhou
Keyword(s):  
Cell Wall ◽  
Cellulose Microfibrils ◽  
Cross Linking ◽  
Cortical Microtubules ◽  
Root Cells ◽  
Left Handedness ◽  
Helical Growth ◽  
Root Waving ◽  
Working Together ◽  
Interesting Behavior

AbstractHelical growth broadly exists in immobile plants to support their limited movement, and Arabidopsis seedling root exhibiting natural left-handedness helical growth is considered as a simplified model for investigating this interesting behavior. Efforts have been made for understanding the mechanism of root helical growth and consequent root waving and skewing on tilted and impenetrable surface, and several models have been established. Here, previous reports are reviewed and a straightforward torsions-driven mechanism has been emphasized, and additional experiments have been performed to fill up the gaps of this theory in our study.This study implies that, torsions originating from handedness of both cortical microtubules and cellulose microfibrils play central role in root handed helical growth. Different from torsions directly provided by handed assembled cortical microtubules, torsions originating from right-handed assembled cellulose microfibrils are relaxed by their cross-linking with pectin within cell wall, but only exhibited when their cross-linking is interrupted due to damaged cell wall integrity. To topologically relax these torsions, supercoils of cortical microtubules and/or cellulose microfibrils exhibiting as oblique alignments are formed in root cells, which alter the orientation of root cell files and generate handed helical roots. Working together with gravitropic response, relaxation of torsions originating from helical roots drives roots to elongate with handedness, which therefore produces waved and skewed roots on tilted and impenetrable surface.

scholarly journals Mechanical stress initiates and sustains the morphogenesis of wavy leaf epidermal cells

2019 ◽  
Author(s):  
Amir J Bidhendi ◽  
Bara Altartouri ◽  
Frédérick P. Gosselin ◽  
Anja Geitmann
Keyword(s):  
Mechanical Properties ◽  
Cell Wall ◽  
Cell Walls ◽  
De Novo ◽  
Epidermal Cells ◽  
Cellulose Microfibrils ◽  
List Type ◽  
Pavement Cells ◽  
Morphogenetic Process ◽  
Cell Shapes

Plant cell morphogenesis is governed by the mechanical properties of the cell wall and the resulting cell shape is intimately related to the respective specific function. Pavement cells covering the surface of plant leaves form wavy interlocking patterns in many plants. We use computational mechanics to simulate the morphogenetic process based on experimentally assessed cell shapes, growth dynamics, and cell wall chemistry. The simulations and experimental evidence suggest a multistep process underlying the morphogenesis of pavement cells during tissue differentiation. The mechanical shaping process relies on spatially confined, feedback-augmented stiffening of the cell wall in the periclinal walls, an effect that correlates with experimentally observed deposition patterns of cellulose and de-esterified pectin. We provide evidence for mechanical buckling of the pavement cell walls that can robustly initiate patternsde novoand may precede chemical and geometrical anisotropy.HighlightsA multistep mechano-chemical morphogenetic process underlies the wavy pattern of epidermal pavement cells.Microtubule polarization is preceded by an event that breaks mechanical isotropy in the cell wall.Mechanical models simulate the formation of wavy cell shapes, predict buckling of the cell walls and spatially confined variations in the mechanical properties of leaf epidermal cells.Stress/strain stiffening following the buckling of the cell walls constitutes a crucial element in a positive feedback loop forming interlocking pavement cells.Polarization of cortical microtubules, cellulose microfibrils, and de-esterified pectin occur at the necks of wavy pavement cells, matching thein silicoprediction of cell wall stiffening.

scholarly journals Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Veronica Giourieva ◽  
Emmanuel Panteris
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Expansion ◽  
Cortical Microtubule ◽  
Root Apex ◽  
Cellulose Synthase ◽  
Cellulose Biosynthesis ◽  
Cortical Microtubules ◽  
Wild Type ◽  
Microtubule Stability

Abstract Background Cortical microtubules regulate cell expansion by determining cellulose microfibril orientation in the root apex of Arabidopsis thaliana. While the regulation of cell wall properties by cortical microtubules is well studied, the data on the influence of cell wall to cortical microtubule organization and stability remain scarce. Studies on cellulose biosynthesis mutants revealed that cortical microtubules depend on Cellulose Synthase A (CESA) function and/or cell expansion. Furthermore, it has been reported that cortical microtubules in cellulose-deficient mutants are hypersensitive to oryzalin. In this work, the persistence of cortical microtubules against anti-microtubule treatment was thoroughly studied in the roots of several cesa mutants, namely thanatos, mre1, any1, prc1-1 and rsw1, and the Cellulose Synthase Interacting 1 protein (csi1) mutant pom2-4. In addition, various treatments with drugs affecting cell expansion were performed on wild-type roots. Whole mount tubulin immunolabeling was applied in the above roots and observations were performed by confocal microscopy. Results Cortical microtubules in all mutants showed statistically significant increased persistence against anti-microtubule drugs, compared to those of the wild-type. Furthermore, to examine if the enhanced stability of cortical microtubules was due to reduced cellulose biosynthesis or to suppression of cell expansion, treatments of wild-type roots with 2,6-dichlorobenzonitrile (DCB) and Congo red were performed. After these treatments, cortical microtubules appeared more resistant to oryzalin, than in the control. Conclusions According to these findings, it may be concluded that inhibition of cell expansion, irrespective of the cause, results in increased microtubule stability in A. thaliana root. In addition, cell expansion does not only rely on cortical microtubule orientation but also plays a regulatory role in microtubule dynamics, as well. Various hypotheses may explain the increased cortical microtubule stability under decreased cell expansion such as the role of cell wall sensors and the presence of less dynamic cortical microtubules.

scholarly journals Consumo alimentar comparativo de larvas de Phytalus sanctipauli Blanch. (Coleoptera: Scarabaeidae)

1997 ◽  
Vol 26 (2) ◽  
pp. 223-228
Author(s):  
Régis S. S. dos Santos ◽  
Lúcia M. G. Diefenbach ◽  
Luiza R. Redaelli ◽  
Dirceu N. Gassen
Keyword(s):  
Triticum Aestivum ◽  
Zea Mays ◽  
Avena Sativa

O consumo alimentar de larvas de 3º estádio de Phytalus sanctipauli Blanch. foi estudado em laboratório. Os insetos foram criados individualmente e alimentados com aveia (Avena sativa), milho (Zea mays) ou trigo (Triticum aestivum) ou com as três espécies juntas. A quantidade (massa seca) ingerida de raiz e semente, respectivamente, foi maior em trigo (28,5 mg), menor em aveia (16,4 mg) e intermediária no milho (22,6 mg). O consumo de folha foi semelhante em aveia (18,2 mg) e trigo (19,8 mg) e significativamente superior ao de milho (2,1 mg). Avaliando-se a plântula como um todo, o consumo de trigo foi superior tanto quando as espécies vegetais foram oferecidas isoladamente (89,8 mg) quanto em conjunto (56,9 mg). O peso fresco médio das larvas ao término da alimentação foi maior em trigo (100,2 mg) e milho (102,2 mg) do que em aveia (85,9 mg). O número médio de plantas destruído por larva foi marcadamente maior em trigo e aveia (27,4 e 24,9, respectivamente) comparado com milho (0,9).

Sign in / Sign up

Export Citation Format

Share Document