Characterization of phospholipid-encapsulated gold nanoparticles: a versatile platform to study drug delivery and cellular uptake mechanisms

2015 ◽  
Vol 93 (2) ◽  
pp. 265-271 ◽  
Author(s):  
Meijing Wang ◽  
Nils O. Petersen
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Cellular Uptake ◽  
Study Drug ◽  
Cellular Systems ◽  
Reaction Conditions ◽  
Overall Stability ◽  
Comprehensive Characterization ◽  
Uptake Mechanisms

The data presented in this work aim to provide a comprehensive characterization of lipid-coated gold nanoparticles. We show that it is possible to envelop gold nanoparticles with a coating of lipids during the formation of the gold nanoparticles, that the gold in these lipid-coated gold nanoparticles is crystalline and the size and shape can be controlled by the reaction conditions (within limits), that the lipid coating corresponds to a thickness consistent with the formation of a bilayer, that the bilayer can include fluorescent probes that, while quenched, can be used to trace the fate of the gold nanoparticles in cellular systems, that their surface charge, and hence their overall stability in solution, is influenced by the lipid coating, and that while some lipid dyes may exchange among the particles, they are sufficiently stable to exchange to permit their use as tracers in cell studies. We believe this is the most comprehensive characterization of these systems to date.

scholarly journals Structural characterization of functionalized gold nanoparticles for drug delivery in cancer therapy: a NMR based approach

2015 ◽  
Vol 17 (29) ◽  
pp. 18971-18979 ◽  
Author(s):  
Sílvia C. Coelho ◽  
Maria Rangel ◽  
Maria C. Pereira ◽  
Manuel A. N. Coelho ◽  
Galya Ivanova
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Cancer Therapy ◽  
Structural Characterization ◽  
Au Nanoparticles ◽  
Functionalized Gold Nanoparticles ◽  
Loaded Surface

Structure of Bortezomib loaded, surface functionalized Au nanoparticles.

Synthesis and characterization of levan hydrogels and their use for resveratrol release

2021 ◽  
pp. 088391152110557
Author(s):  
Sinem Selvin Selvi ◽  
Merve Erginer Hasköylü ◽  
Seval Genç ◽  
Ebru Toksoy Öner
Keyword(s):  
Drug Delivery ◽  
Human Keratinocytes ◽  
Diglycidyl Ether ◽  
Adhesive Properties ◽  
Test Analysis ◽  
Culture Studies ◽  
Chemical Derivatives ◽  
Release Studies ◽  
Comprehensive Characterization

Considering the need for systematic studies on levan based hydrogels to widen their use in drug delivery systems and biomedical applications, this study is mainly focused on the synthesis and comprehensive characterization as well as drug release properties of hydrogels based on Halomonas levan (HL) and its chemical derivatives. For this, hydrolyzed and phosphonated HL derivatives were chemically synthesized and then cross-linked with 1,4-Butanediol diglycidyl ether (BDDE) and the obtained hydrogels were characterized in terms of their swelling, adhesivity, and rheological properties. Both native and phosphonated HL hydrogels retained their rigid gel like structure with increasing shear stress levels and tack test analysis showed superior adhesive properties of the phosphonated HL hydrogels. Moreover, hydrogels were loaded with resveratrol and entrapment and release studies as well as cell culture studies with human keratinocytes were performed. Biocompatible and adhesive features of the hydrogels confirmed their suitability for tissue engineering and drug delivery applications.

Synthesis and characterization of Arabic gum capped gold nanoparticles for tumor-targeted drug delivery

2015 ◽  
Vol 139 ◽  
pp. 241-244 ◽  
Author(s):  
P. Renuga Devi ◽  
C. Senthil Kumar ◽  
P. Selvamani ◽  
N. Subramanian ◽  
K. Ruckmani
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Targeted Drug Delivery ◽  
Synthesis And Characterization ◽  
Arabic Gum ◽  
Targeted Drug

scholarly journals CARMUSTINE LOADED LACTOFERRIN NANOPARTICLES DEMONSTRATES AN ENHANCED ANTIPROLIFERATIVE ACTIVITY AGAINST GLIOBLASTOMA IN VITRO

2018 ◽  
Vol 10 (6) ◽  
pp. 234 ◽  
Author(s):  
Harikiran Athmakur ◽  
Anand Kumar Kondapi
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Cellular Uptake ◽  
Stable Distribution ◽  
Drug Loading ◽  
Glioblastoma Cells ◽  
Treatment Regimens ◽  
Microscopic Studies

Objective: Despite sophisticated treatment regimens, there is no significant improvement in the mortality rates of glioblastoma due to insufficient dosage delivery, reoccurrence of tumors, higher systemic toxicity, etc. Since brain endothelial cells and glioblastoma cells express lactoferrin receptors, a target-specific drug delivery vehicle was developed using lactoferrin itself as a matrix, into which carmustine was loaded. The objective was to use carmustine loaded lactoferrin nanoparticles (CLN) to achieve higher therapeutic efficacy and target specificity compared to free carmustine.Methods: CLN were prepared using the Sol-oil method. The nanoparticles prepared were characterized for their size, shape, polydispersity, and stability using FESEM and DLS methods. Drug loading and drug releasing efficiencies were also estimated. Further, cellular uptake of nanoparticles and their antiproliferative efficacy against glioblastoma cells were evaluated.Results: Characterization of CLN showed that they were spherical with ≤ 41 nm diameter and exhibited homogeneously dispersed stable distribution. Loading efficiency of carmustine in CLN was estimated to be 43±3.7 %. Drug release from the nanoparticles was pH dependent with the maximum observed at pH 5. At physiological and gastric pH, drug release was lower, whereas maximum release was observed at endocytotic vesicular and around tumor extracellular pH. Confocal microscopic studies showed an active cellular uptake of nanoparticles. Results of antiproliferative analysis substantiated a higher antiproliferative effect for CLN compared to free carmustine.Conclusion: The results of the study demonstrated that CLN serves as a vital tool, in designing an effective treatment strategy for targeted drug delivery to glioblastoma.

scholarly journals Purification, and Characterization of Polysaccharides of Some Plants and Utilized in Nanocarriers

2020 ◽  
Vol 2 (1) ◽  
pp. 63
Keyword(s):  
Drug Delivery ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Study Drug ◽  
Purification And Characterization

In this study, drug-loaded nanocarrier made of plant gum polysaccharides was used for in vitro drug delivery. Purified gum was subjected to characterization and nanocarriers synthesis. Purified polysaccharides were having good antioxidant activity and antibacterial activity. The nanocarriers were synthesized using STMP, where the size was around 200 nm. It was delivering the drug good and possessing antibacterial activity.

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