A novel rhodamine-based fluorescent probe for selective detection of ClO– and its application in living cell imaging

2018 ◽  
Vol 96 (12) ◽  
pp. 1129-1133
Author(s):  
Changping Yang ◽  
Mingliang Zuo ◽  
Xiaoli Hu ◽  
Xuelin Chen ◽  
Duoduo Zhang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Colour Change ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Selective Detection ◽  
Living Cell Imaging ◽  
Interfering Ions

A novel fluorescent rhodamine-based probe L for selective responding to ClO– has been synthesized and characterized. The spectroscopy showed that probe L can detect ClO– in aqueous solution without interaction with other interfering ions, and the detection is also evident by the colour change from colourless to reddish purple under white light. The remarkable fluorescence enhancement showed the high selectivity and sensitivity of probe L for the detection of ClO–. Furthermore, probe L was applied to intracellular fluorescent imaging of HeLa cells treated with ClO– and MTT assay showed nontoxicity in living cells.

scholarly journals Nile-Red-Based Fluorescence Probe for Selective Detection of Biothiols, Computational Study, and Application in Cell Imaging

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4718
Author(s):  
Xiang Rong ◽  
Zhong-Yong Xu ◽  
Jin-Wu Yan ◽  
Zhi-Zhong Meng ◽  
Bin Zhu ◽  
...  
Keyword(s):  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Fluorescence Probe ◽  
Color Change ◽  
Computational Study ◽  
Theoretical Calculations ◽  
Nile Red ◽  
Blue Shift ◽  
Selective Detection

A new colorimetric and fluorescence probe NRSH based on Nile-red chromophore for the detection of biothiols has been developed, exhibiting high selectivity towards biothiols over other interfering species. NRSH shows a blue shift in absorption peak upon reacting with biothiols, from 587 nm to 567 nm, which induces an obvious color change from blue to pink and exhibits a 35-fold fluorescence enhancement at 645 nm in red emission range. NRSH displays rapid (<1 min) response for H2S, which is faster than other biothiols (>5 min). The detection limits of probe NRSH towards biothiols are very low (22.05 nM for H2S, 34.04 nM for Cys, 107.28 nM for GSH and 113.65 nM for Hcy). Furthermore, NRSH is low cytotoxic and can be successfully applied as a bioimaging tool for real-time monitoring biothiols in HeLa cells. In addition, fluorescence mechanism of probe NRSH is further understood by theoretical calculations.

A new rhodamine-derived fluorescent chemodosimeter for Cu2+in aqueous solution and its application in living cell imaging

RSC Advances ◽  
2014 ◽  
Vol 4 (86) ◽  
pp. 45791-45796 ◽  
Author(s):  
Ying-Ying Zhu ◽  
Xuan Zhang ◽  
Hua Guo ◽  
Zhi-Jia Zhu
Keyword(s):  
Aqueous Solution ◽  
Living Cell ◽  
Cell Imaging ◽  
Living Cells ◽  
Hydrolysis Mechanism ◽  
Living Cell Imaging ◽  
Fluorescent Chemodosimeter

A new rhodamine-derived fluorescent chemodosimeter for imaging of Cu2+in living cells was developed under a coordination-promoted hydrolysis mechanism.

Colorimetric and Fluorescent Probe for Hypochlorite with High Selectivity

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
High Selectivity ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Turn On

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).

scholarly journals A highly selective ratiometric fluorescent probe for the cascade detection of Zn2+ and H2PO4− and its application in living cell imaging

RSC Advances ◽  
2017 ◽  
Vol 7 (64) ◽  
pp. 40615-40620 ◽  
Author(s):  
Kui Du ◽  
Shizhen Niu ◽  
Li Qiao ◽  
Yandong Dou ◽  
Qing Zhu ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Fluorescent Probe ◽  
Living Cell ◽  
Cell Imaging ◽  
High Selectivity ◽  
Ratiometric Sensor ◽  
Intermolecular Charge Transfer ◽  
Living Cell Imaging ◽  
Ratiometric Fluorescent Probe

A simple ratiometric sensor (L1) for the cascade detection of Zn2+ and H2PO4− with high selectivity was reported based on the intermolecular charge transfer (ICT) mechanism.

Development of a near-infrared ratiometric fluorescent probe for glutathione using an intramolecular charge transfer signaling mechanism and its bioimaging application in living cells

2019 ◽  
Vol 7 (5) ◽  
pp. 809-814 ◽  
Author(s):  
Yong Zhou ◽  
Li Zhang ◽  
Xuan Zhang ◽  
Zhi-Jia Zhu
Keyword(s):  
Aqueous Solution ◽  
Charge Transfer ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Intramolecular Charge Transfer ◽  
Living Cells ◽  
Selective Detection ◽  
Signaling Mechanism ◽  
Acetyl Group ◽  
Ratiometric Fluorescent Probe

A novel NIR ratiometric fluorescent probe HBT-GSH was developed for the selective detection of GSH over Cys and Hcy in aqueous solution. The signaling mechanism of the GSH-induced removal of an acetyl group and the switching on of ICT fluorescence was proposed.

scholarly journals A highly selective fluorescent probe for the sensing of Cu2+ based on the hydrolysis of a quinoline-2-carboxylate and its application in cell imaging

2020 ◽  
pp. 174751982097392
Author(s):  
Qihua You ◽  
Yihua Zhuo ◽  
Yadong Feng ◽  
Yujuan Xiao ◽  
Yanyu Zhang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Cell Imaging ◽  
High Selectivity ◽  
Living Cells ◽  
World Health ◽  
Strong Fluorescence ◽  
The World ◽  
Phenolic Group ◽  
Health Organization ◽  
Hydrolysis Of

A highly selective OFF–ON fluorescent probe is developed for the sensing of Cu2+ based on the hydrolysis of a quinoline-2-carboxylate moiety. The probe is weakly fluorescent due to esterification of the phenolic group. Upon treatment with 1 equiv. of Cu2+, the probe exhibits strong fluorescence at 570 nm. The probe also exhibits high selectivity for Cu2+ over other cations with a low detection limit of 0.2 μM, which is sensitive enough to meet the standard of the World Health Organization for Cu2+ in drinking water (30 μM). Moreover, the probe shows a very low cell cytotoxicity, and imaging experiments demonstrate that the probe can be used for the sensing of Cu2+ in living cells.

A 1,8-naphthalimide-based fluorescent sensor with high selectivity and sensitivity for Hg2+ in aqueous solution and living cells

2019 ◽  
Vol 11 (25) ◽  
pp. 3150-3154 ◽  
Author(s):  
Daying Liu ◽  
Hualing Zhu ◽  
Jun Shi ◽  
Xinxin Deng ◽  
Tingting Zhang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
High Selectivity ◽  
Fluorescent Sensor ◽  
Picolinic Acid ◽  
Iminodiacetic Acid ◽  
Living Cells

A new 4-amino-1,8-naphthalimide-based fluorescent sensor, with iminodiacetic acid and picolinic acid as receptors, was developed and applied successfully to detect Hg2+ in aqueous solution and living cells.

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