scholarly journals Nile-Red-Based Fluorescence Probe for Selective Detection of Biothiols, Computational Study, and Application in Cell Imaging

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4718
Author(s):  
Xiang Rong ◽  
Zhong-Yong Xu ◽  
Jin-Wu Yan ◽  
Zhi-Zhong Meng ◽  
Bin Zhu ◽  
...  
Keyword(s):  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Fluorescence Probe ◽  
Color Change ◽  
Computational Study ◽  
Theoretical Calculations ◽  
Nile Red ◽  
Blue Shift ◽  
Selective Detection

A new colorimetric and fluorescence probe NRSH based on Nile-red chromophore for the detection of biothiols has been developed, exhibiting high selectivity towards biothiols over other interfering species. NRSH shows a blue shift in absorption peak upon reacting with biothiols, from 587 nm to 567 nm, which induces an obvious color change from blue to pink and exhibits a 35-fold fluorescence enhancement at 645 nm in red emission range. NRSH displays rapid (<1 min) response for H2S, which is faster than other biothiols (>5 min). The detection limits of probe NRSH towards biothiols are very low (22.05 nM for H2S, 34.04 nM for Cys, 107.28 nM for GSH and 113.65 nM for Hcy). Furthermore, NRSH is low cytotoxic and can be successfully applied as a bioimaging tool for real-time monitoring biothiols in HeLa cells. In addition, fluorescence mechanism of probe NRSH is further understood by theoretical calculations.

A novel rhodamine-based fluorescent probe for selective detection of ClO– and its application in living cell imaging

2018 ◽  
Vol 96 (12) ◽  
pp. 1129-1133
Author(s):  
Changping Yang ◽  
Mingliang Zuo ◽  
Xiaoli Hu ◽  
Xuelin Chen ◽  
Duoduo Zhang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Colour Change ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Selective Detection ◽  
Living Cell Imaging ◽  
Interfering Ions

A novel fluorescent rhodamine-based probe L for selective responding to ClO– has been synthesized and characterized. The spectroscopy showed that probe L can detect ClO– in aqueous solution without interaction with other interfering ions, and the detection is also evident by the colour change from colourless to reddish purple under white light. The remarkable fluorescence enhancement showed the high selectivity and sensitivity of probe L for the detection of ClO–. Furthermore, probe L was applied to intracellular fluorescent imaging of HeLa cells treated with ClO– and MTT assay showed nontoxicity in living cells.

scholarly journals Synthesis of a Flavone Based Fluorescent Probe Bearing a Nitroolefin Moiety for Selective Detection of Cysteine

2018 ◽  
Vol 23 (2) ◽  
pp. 85
Author(s):  
Al-Busafi Saleh N. ◽  
Al-Hamami Nadia M. ◽  
O. Suliman Fakhr Eldin ◽  
Varghese Beena
Keyword(s):  
Fluorescent Probe ◽  
1H Nmr Spectroscopy ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Blue Shift ◽  
Selective Detection ◽  
Normal Human ◽  
The Michael Addition ◽  
Slight Blue Shift ◽  
Cysteine Level

 In this paper, we report the synthesis, characterization and investigation of the optical properties of a new fluorescent probe, 4ʹ-nitroolefinylflavone (4ʹ-NOF) 1, based on a flavone skeleton and bearing a nitroolefin moiety. Upon the addition of cysteine, a remarkable fluorescence enhancement (200 fold) was observed for probe 1 accompanied with a slight blue shift from 457 nm to 459 nm.  The prepared probe displays high selectivity and sensitivity towards cysteine over other amino acids. The Michael addition of cysteine to probe 1 was confirmed by 1H-NMR spectroscopy. The detection limit of probe 1 towards cysteine was found to be 1.63 μΜ, a lower concentration than the normal human cysteine level, 30-200 μΜ.  

A FRET based colorimetric and fluorescence probe for selective detection of Bi3+ ion and live cell imaging

2018 ◽  
Vol 360 ◽  
pp. 26-33 ◽  
Author(s):  
Susanta Adhikari ◽  
Sandip Mandal ◽  
Avijit Ghosh ◽  
Subhajit Guria ◽  
Abhishek Pal ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescence Probe ◽  
Live Cell ◽  
Selective Detection

A highly selective fluorescent ‘turn-on’ chemosensor for Hg2+ based on a phthalazin-hydrazone derivative and its application in human cervical cancer cell imaging

2015 ◽  
Vol 39 (4) ◽  
pp. 3071-3076 ◽  
Author(s):  
Dipesh Mahajan ◽  
Nilesh Khairnar ◽  
Banashree Bondhopadhyay ◽  
Suban K. Sahoo ◽  
Anupam Basu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cell ◽  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Cervical Cancer Cell ◽  
Turn On ◽  
Human Cervical Cancer Cell ◽  
Hydrazone Derivative ◽  
Human Cervical Cancer

Sensor 1 showed a high selectivity and sensitivity towards Hg2+ by giving significant fluorescence enhancement.

A Novel Material Fluorescence Probe Based on Covalently Functionalized Graphene Oxide by Aminocoumarin for Detection of CN−

NANO ◽  
2020 ◽  
Vol 15 (02) ◽  
pp. 2050022
Author(s):  
Wenhui Ma ◽  
Zhaochuan Yu ◽  
Zhongcai Li ◽  
Jing Wen ◽  
Baoru Zhao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Spectra ◽  
High Selectivity ◽  
Fluorescence Probe ◽  
Deionized Water ◽  
Selective Detection ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
The World ◽  
Novel Material

Owing to the hazardness, the sensitive and selective detection of cyanide (CN−) in water or water-containing systems has still been a puzzle plaguing scientists all around the world. In this paper, a novel fluorescence probe based on covalently functionalized graphene oxide (GO) by aminocoumarin for highly selective detection of CN− in deionized water was prepared under the action of EDC/NHS. This probe bears aminocoumarin group as the recognition site and fluorophore, and its fluorescence quantum yield is increased by 0.07. Simultaneously, by loading on GO, the dispersibility, selectivity and reversibility were soundly improved, which facilitated the detection process. The fluorescence spectra showed that the probe has high selectivity and sensitivity for cyanide, and has potential application in real water.

Dihydropyridine-based fluorescence probe for nitric oxide

RSC Advances ◽  
2016 ◽  
Vol 6 (89) ◽  
pp. 85698-85703 ◽  
Author(s):  
Su-Fang Ma ◽  
Qiu-Hua Wang ◽  
Fu-Tao Liu ◽  
Hui-Li Wang ◽  
De-Cai Fang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Probe

A fluorescence probe for detecting nitric oxide based on dihydropyridine showed high selectivity and sensitivity as well as cell imaging ability.

A “Turn‐On” Fluorescence Probe for Selective Detection of Al 3+ in Aqueous Environment: Crystal Structure, Theoretical and Cell Imaging Studies

2020 ◽  
Vol 5 (7) ◽  
pp. 2284-2290 ◽  
Author(s):  
Shraddha Rani Gupta ◽  
Pranjalee Yadav ◽  
Priya Singh ◽  
Biplob Koch ◽  
Vinod P. Singh
Keyword(s):  
Crystal Structure ◽  
Cell Imaging ◽  
Fluorescence Probe ◽  
Aqueous Environment ◽  
Selective Detection ◽  
Imaging Studies ◽  
Turn On

Proton donor modulating ESIPT-based fluorescent probes for highly sensitive and selective detection of Cu2+

RSC Advances ◽  
2015 ◽  
Vol 5 (93) ◽  
pp. 76296-76301 ◽  
Author(s):  
Liyan Huang ◽  
Biao Gu ◽  
Wei Su ◽  
Peng yin ◽  
Haitao Li
Keyword(s):  
Fluorescent Probes ◽  
Cell Imaging ◽  
High Selectivity ◽  
Proton Donor ◽  
Selective Detection ◽  
Probe Molecules ◽  
Highly Sensitive

Two novel ESIPT-based fluorescent probes for Cu2+ detection were developed. Altering the linker in probe molecules reversed their sensing behavior. Both probes exhibited high selectivity and sensitivity to Cu2+, and can be used for cell imaging.

scholarly journals A Reversible Colorimetric and Fluorescence “Turn-Off” Chemosensor for Detection of Cu2+ and Its Application in Living Cell Imaging

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4283 ◽  
Author(s):  
Yun Hu ◽  
Aiqian Chen ◽  
Zhuo Kong ◽  
Demeng Sun
Keyword(s):  
Cell Imaging ◽  
High Selectivity ◽  
Color Change ◽  
Dual Function ◽  
Biological Applications ◽  
Sensing System ◽  
Neutral Aqueous Solution ◽  
Intracellular Changes ◽  
Living Cell Imaging ◽  
Naked Eye Detection

Dual-function chemosensors that combine the capability of colorimetric and fluorimetric detection of Cu2+ are still relatively rare. Herein, we report that a 3-hydroxyflavone derivative (E)-2-(4-(dimethylamino)styryl)-3-hydroxy-4H-chromen-4-one (4), which is a red-emitting fluorophore, could serve as a reversible colorimetric and fluorescence “turn-off” chemosensor for the detection of Cu2+. Upon addition of Cu2+ to 4 in neutral aqueous solution, a dramatic color change from yellow to purple-red was clearly observed, and its fluorescence was markedly quenched, which was attributed to the complexation between the chemosensor and Cu2+. Conditions of the sensing process had been optimized, and the sensing studies were performed in a solution of ethanol/phosphate buffer saline (v/v = 3:7, pH = 7.0). The sensing system exhibited high selectivity towards Cu2+. The limit of naked eye detection of Cu2+ was determined at 8 × 10−6 mol/L, whereas the fluorescence titration experiment showed a detection limit at 5.7 × 10−7 mol/L. The complexation between 4 and Cu2+ was reversible, and the binding constant was found to be 3.2 × 104 M−1. Moreover, bioimaging experiments showed that 4 could penetrate the cell membrane and respond to the intracellular changes of Cu2+ within living cells, which indicated its potential for biological applications.

Synthesis of a BODIPY disulfonate near-infrared fluorescence-enhanced probe with high selectivity to endogenous glutathione and two-photon fluorescent turn-on through thiol-induced SNAr substitution

2018 ◽  
Vol 6 (19) ◽  
pp. 3023-3029 ◽  
Author(s):  
Xiang Xia ◽  
Ying Qian ◽  
Baoxing Shen
Keyword(s):  
Near Infrared ◽  
High Selectivity ◽  
Fluorescence Probe ◽  
Selective Detection ◽  
Near Infrared Fluorescence ◽  
Two Photon ◽  
Turn On

A BODIPY disulfonate BODIPY-diONs with two-photon fluorescent turn-on effect was developed as fluorescence probe for selective detection of glutathione over cysteine and homocysteine.

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