Comparative transcriptome analysis of the hepatopancreas of Eriocheir sinensis following oral gavage with enrofloxacin

Enrofloxacin is an important drug that is widely used in the treatment of the diseased Eriocheir sinensis. This study compared transcriptome differences in the hepatopancreas of E. sinensis following oral gavage with enrofloxacin. Our study produced 80 228 728 and 88 888 706 raw reads from control and treatment groups, and after filtering and quality checks of the raw sequence reads, our analysis yielded 78 843 613 and 87 628 922 clean reads with a mean length of 126 bp from control and treatment groups, respectively. A total of 15 797 transcripts were assembled, with 11 975 transcripts annotated. Moreover, 2795 transcripts were judged to be differentially expressed genes. Gene ontology terms “biological process” and “metabolic process” were the most enriched in the oxidation–reduction process, translational initiation, membrane, cytoplasmic part, and hydrolase activity. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that metabolic and signal transduction pathways were significantly enriched. Furthermore, we found that gshB and the CYP450 enzyme system plays a role in the metabolism of enrofloxacin in the hepatopancreas of E. sinensis. This study identified differential transcripts related to transmembrane transport and drug metabolism in E. sinensis that could help develop understanding of the molecular basis of enrofloxacin metabolism in this economically important aquaculture species.

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Transcriptomic Changes Induced by Drought Stress in Hardneck Garlic during the Bolting/Bulbing Stage

Agronomy ◽

10.3390/agronomy11020246 ◽

2021 ◽

Vol 11 (2) ◽

pp. 246

Author(s):

Xiangjun Zhou ◽

Jorge Alberto Condori-Apfata ◽

Xiaoqin Liu ◽

Sandro Jhonatan Condori-Pacsi ◽

Maria Valderrama Valencia ◽

...

Keyword(s):

Drought Stress ◽

Biological Process ◽

Reduction Process ◽

Leaf Water Content ◽

Monocotyledonous Plant ◽

Oxidation Reduction ◽

Taste And Odor ◽

Genes Encoding ◽

Shock Proteins ◽

Transcriptomic Changes

Garlic (Allium sativum L.) is an economically important, monocotyledonous plant with a strong taste and odor. Drought stress adversely affects its growth, development, and yield, particularly during the bolting/bulbing stage. Herein we performed RNA-seq to assess transcriptomic changes induced by drought stress in bolting/bulbing hardneck garlic plants (Purple Glazer). We observed that drought stress significantly reduced photosynthesis rate, fresh weight, and leaf water content. Transcriptomic analysis of garlic leaves under normal conditions and drought stress led to the identification of 5215 differentially expressed genes (2748 up- and 2467 downregulated). The upregulated DEGs were primarily involved in “biological process”, “metabolic process”, “oxidation-reduction process”, carbohydrate and lipid metabolism, and “proteolysis”, whereas the downregulated DEGs were mainly involved in “biological process” and metabolism of various molecules. In addition, genes encoding abscisic acid biosynthetic and catabolic enzymes, heat shock proteins, and E3 ubiquitin ligases were significantly altered by drought stress, indicating involvement in drought tolerance. A further comparison with the DEGs related to salinity stress-treated garlic revealed 867 and 305 DEGs with a similar and reverse expression alteration tendency, respectively.

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Solution blowing spinning technology and plasma-assisted oxidation-reduction process toward green development of electrically conductive cellulose nanofibers

Environmental Science and Pollution Research ◽

10.1007/s11356-021-14615-w ◽

2021 ◽

Author(s):

Hanadi A. Katouah ◽

Refat El-Sayed ◽

Nashwa M. El-Metwaly

Keyword(s):

Cellulose Nanofibers ◽

Reduction Process ◽

Electrically Conductive ◽

Solution Blowing ◽

Oxidation Reduction ◽

Green Development

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Improved Joint Strength with Sintering Bonding Using Microscale Cu Particles by an Oxidation-Reduction Process

2016 IEEE 66th Electronic Components and Technology Conference (ECTC) ◽

10.1109/ectc.2016.248 ◽

2016 ◽

Cited By ~ 2

Author(s):

Xiangdong Liu ◽

Hiroshi Nishikawa

Keyword(s):

Joint Strength ◽

Reduction Process ◽

Oxidation Reduction

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Unusual Wetting Behavior of Liquid Metals on Porous Layer Formed at Surface of Iron Substrate Prepared by Oxidation-Reduction Process

Materials Science Forum ◽

10.4028/www.scientific.net/msf.561-565.1699 ◽

2007 ◽

Vol 561-565 ◽

pp. 1699-1701

Author(s):

Nobuyuki Takahira ◽

Takeshi Yoshikawa ◽

Toshihiro Tanaka

Keyword(s):

Liquid Metal ◽

Porous Layer ◽

Liquid Metals ◽

Reduction Process ◽

Surface Oxidation ◽

Porous Iron ◽

Normal Contact ◽

Wetting Behavior ◽

Oxidation Reduction ◽

Oxidized Iron

Unusual wetting behavior of liquid Cu was found on a surface-oxidized iron substrate in reducing atmosphere. Liquid Cu wetted and spread very widely on the iron substrate when a droplet was attached with the substrate in Ar-10%H2 after the surface oxidation of the substrate. The oxidationreduction process fabricates a porous layer at the surface of the iron substrate. The pores in the porous iron layer are 3-dimensionally interconnected. Thus, liquid metals, which are contacted with the reduced iron samples, penetrate into these pores by capillary force to cause the unusual wetting behavior. It has been already confirmed that liquid Ag, Sn, In and Bi show this phenomenon onto surface-porous iron samples as well as liquid Cu. This unusual wetting behavior of a liquid metal has been correlated to the normal contact angle of the liquid metal on a flat iron substrate.

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Performance of poly-o-phenylenediamine and its carbon dot composite electrode in the removal of Cu2+ from its aqueous solution

Functional Materials Letters ◽

10.1142/s1793604721510371 ◽

2021 ◽

pp. 2151037

Author(s):

Yu Meng ◽

Qing Zhong ◽

Arzugul Muslim

Keyword(s):

Aqueous Solution ◽

Electrochemical Reduction ◽

Composite Electrode ◽

Reduction Process ◽

Order Kinetic ◽

Carbon Dot ◽

First Order ◽

Oxidation Reduction ◽

Order Kinetic Model ◽

Optimal Ph

Because −NH2 and −NH− in poly-[Formula: see text]-phenylenediamine (P[Formula: see text]PD) can interact strongly with the empty orbitals of Cu to show unique electrochemical activity, P[Formula: see text]PD is suitable for the removal of Cu[Formula: see text] by electrochemical oxidation–reduction process. In this study, with P[Formula: see text]PD and its carbon dot composite (CDs/P[Formula: see text]PD) as working electrodes, the electrochemical reduction and removal of Cu[Formula: see text] in the aqueous solution were carried out with the potentiostatic method. According to effects of voltage, pH of the solution, initial concentration of Cu[Formula: see text], and electrochemical reduction time on the Cu[Formula: see text] removal, the Cu[Formula: see text] removal ratios of P[Formula: see text]PD and CDs/P[Formula: see text]PD were up to 64.69% and 73.34%, respectively, at −0.2 V and the optimal pH. Additionally, results showed that these processes were in line with the quasi-first order kinetic model. Both P[Formula: see text]PD and CDs/P[Formula: see text]PD showed good reproducibility in six cycles. After five times of repeated usage, the regeneration efficiencies of P[Formula: see text]PD and CDs/P[Formula: see text]PD dropped to 77.04% and 79.36%, respectively.

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Alternating copolymerization of cyclic germylenes with N-phenyl-p-quinoneimine via oxidation-reduction process

Polymer Journal ◽

10.1038/pj.2016.59 ◽

2016 ◽

Vol 48 (9) ◽

pp. 969-972

Author(s):

Hiroshi Takano ◽

Masafumi Hiraishi ◽

Shigeru Yaguchi ◽

Satoru Iwata ◽

Shin-ichiro Shoda ◽

...

Keyword(s):

Reduction Process ◽

Oxidation Reduction ◽

Alternating Copolymerization

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Comprehensive Analysis of lncRNAs and circRNAs Reveals the Metabolic Specialization in Oxidative and Glycolytic Skeletal Muscles

International Journal of Molecular Sciences ◽

10.3390/ijms20122855 ◽

2019 ◽

Vol 20 (12) ◽

pp. 2855 ◽

Cited By ~ 3

Author(s):

Linyuan Shen ◽

Mailin Gan ◽

Qianzi Tang ◽

Guoqing Tang ◽

Yanzhi Jiang ◽

...

Keyword(s):

Meat Quality ◽

Skeletal Muscles ◽

Target Genes ◽

Fiber Type ◽

Muscle Metabolism ◽

Reduction Process ◽

Differentially Expressed ◽

Rna Seq ◽

Oxidation Reduction ◽

Regulatory Analysis

The biochemical and functional differences between oxidative and glycolytic muscles could affect human muscle health and animal meat quality. However, present understanding of the epigenetic regulation with respect to lncRNAs and circRNAs is rudimentary. Here, porcine oxidative and glycolytic skeletal muscles, which were at the growth curve inflection point, were sampled to survey variant global expression of lncRNAs and circRNAs using RNA-seq. A total of 4046 lncRNAs were identified, including 911 differentially expressed lncRNAs (p < 0.05). The cis-regulatory analysis identified target genes that were enriched for specific GO terms and pathways (p < 0.05), including the oxidation-reduction process, glycolytic process, and fatty acid metabolic. All these were closely related to different phenotypes between oxidative and glycolytic muscles. Additionally, 810 circRNAs were identified, of which 137 were differentially expressed (p < 0.05). Interestingly, some circRNA-miRNA-mRNA networks were found, which were closely linked to muscle fiber-type switching and mitochondria biogenesis in muscles. Furthermore, 44.69%, 39.19%, and 54.01% of differentially expressed mRNAs, lncRNAs, and circRNAs respectively were significantly enriched in pig quantitative trait loci (QTL) regions for growth and meat quality traits. This study reveals a mass of candidate lncRNAs and circRNAs involved in muscle physiological functions, which may improve understanding of muscle metabolism and development from an epigenetic perspective.

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