The diversity and antimicrobial activity of endophytic fungi associated with medicinal plant Baccharis trimera (Asteraceae) from the Brazilian savannah

2014 ◽  
Vol 60 (12) ◽  
pp. 847-856 ◽  
Author(s):  
Mariana L.A. Vieira ◽  
Susana Johann ◽  
Frederic M. Hughes ◽  
Carlos A. Rosa ◽  
Luiz H. Rosa
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Endophytic Fungi ◽  
Folk Medicine ◽  
Abundant Species ◽  
Antimicrobial Activities ◽  
Fungal Endophyte ◽  
Bioactive Metabolites ◽  
Brazilian Savannah ◽  
Baccharis Trimera

The fungal endophyte community associated with Baccharis trimera, a Brazilian medicinal plant, was characterized and screened for its ability to present antimicrobial activity. By using molecular methods, we identified and classified the endophytic fungi obtained into 25 different taxa from the phyla Ascomycota and Basidiomycota. The most abundant species were closely related to Diaporthe phaseolorum, Pestalotiopsis sp. 1, and Preussia pseudominima. The differences observed in endophytic assemblages from different B. trimera specimens might be associated with their crude extract activities. Plants that had higher α-biodiversity were also those that contributed more to the regional (γ) diversity. All fungal isolates were cultured and their crude extracts screened to examine the antimicrobial activities. Twenty-three extracts (12.8%) displayed antimicrobial activities against at least one target microorganism. Among these extracts, those obtained from Epicoccum sp., Pestalotiopsis sp. 1, Cochliobolus lunatus, and Nigrospora sp. presented the best minimum inhibitory concentration values. Our results show that the endophytic fungal community associated with the medicinal plant B. trimera included few dominant bioactive taxa, which may represent sources of compounds with antifungal activity. Additionally, the discovery of these bioactive fungi in association with B. trimera suggests that Brazilian plants used as folk medicine may shelter a rich fungal diversity as well as taxa able to produce bioactive metabolites with antimicrobial activities.

scholarly journals Isolation, diversity, and antimicrobial activity of fungal endophytes from Rohdea chinensis (Baker) N.Tanaka (synonym Tupistra chinensis Baker) of Qinling Mountains, China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9342
Author(s):  
Chao An ◽  
Saijian Ma ◽  
Xinwei Shi ◽  
Wenjiao Xue ◽  
Chen Liu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
High Performance ◽  
Fungal Endophytes ◽  
Folk Medicine ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Qinling Mountains

Endophytic fungi have been emerged as fruitful resources for producing structurally fascinating and biologically active secondary metabolites. However, endophytic fungi from medicinal plants of Qinling Mountains–the most important natural climatic boundary between the subtropical and warm temperate zones of China with an astonishingly high level of biodiversity–have rarely been explored as potential sources of novel fungal species and active secondary metabolites. In this study, a total of 371 fungal colonies were successfully isolated from 510 tissue segments of the medicinal Tupistra chinensis Baker collected from Qinling Mountains, China. Roots of T. chinensis Baker are used as a folk medicine to ameliorate pharyngitis and treat rheumatic diseases. A total of 100 representative morphotype strains were identified according to ITS rDNA sequence analyses and were grouped into three phyla (Ascomycota, Basidiomycota, Mucoromycota), seven classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Microbotryomycetes, Agaricomycetes, Leotiomycetes, Mortierellomycetes), and at least 35 genera. The genera of Collectotrichum (IF, 29.92%), Fusarium (IF, 8.36%), Aspergillus (IF, 8.09%), and Dactylonectria (IF, 5.39%) were most frequently isolated from the tissues of T. chinensis Baker. The Species Richness Index (S, 65) and the Shannon-Wiener Index (H′, 3.7914) indicated that T. chinensis Baker harbored abundant fungal resources. Moreover, five isolates were potential new taxa because of low similarity of ITS sequences ranged from 95.09%∼96.61%. Fifteen out of 100 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. Two important lead compounds produced by two stains (F8047 and F8075) with high antimicrobial activities were identified using high performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC–QTOF MS) analyses. In addition, it was noteworthy that the strain F8001, which may be a potential new species, showed antimicrobial activity and should be investigated further. Overall, these results indicated that the endophytic fungi from T. chinensis Baker could be exploited as a novel source of bioactive compounds.

Diversity and antimicrobial activities of the fungal endophyte community associated with the traditional Brazilian medicinal plant Solanum cernuum Vell. (Solanaceae)

2012 ◽  
Vol 58 (1) ◽  
pp. 54-66 ◽  
Author(s):  
Mariana L.A. Vieira ◽  
Alice F.S. Hughes ◽  
Viviane B. Gil ◽  
Aline B.M. Vaz ◽  
Tânia M.A. Alves ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Endophytic Fungi ◽  
Colletotrichum Gloeosporioides ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Fungal Endophyte ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
Promising Source ◽  
Endophyte Community

The diversity and antimicrobial activity of endophytic fungi associated with the Brazilian medicinal plant Solanum cernuum Vell. were studied during summer and winter seasons. A total of 246 fungal isolates were obtained, including 225 filamentous fungi and 21 yeasts. They were identified by morphological, physiological, and molecular methods. Fifty-five different taxa represented by the phyla Ascomycota (33 taxa), Basidiomycota (21 taxa), and Zygomycota (one taxon) were identified. The most abundant taxa were closely related to Arthrobotrys foliicola , Colletotrichum gloeosporioides , Coprinellus radians , Glomerella acutata , Diatrypella frostii , Phoma glomerata , Mucor sp., Phlebia subserialis , Phoma moricola , Phanerochaete sordida , and Colletotrichum sp. A total of 265 fungal extracts were screened and 64 (26.01%) displayed antimicrobial activities. Among these extracts, 18 (28.12%) presented antibacterial and antifungal activities, 42 (65.62%) displayed selective antibacterial activity, and four (6.25%) exhibited only antifungal activity. The best values of minimum inhibitory concentration were obtained from extracts of Cryptococcus rajasthanensis , Glomerella acutata, Leptosphaeria sp., and Phoma glomerata ranging from 7.8 to 15.62 µg/mL. This study is the first survey of the endophytic fungi community associated with S. cernuum, and our results show that they can represent a promising source of bioactive compounds.

Endophytic fungi from the medicinal plant Lippia sidoides Cham. and their antimicrobial activity

Symbiosis ◽  
2011 ◽  
Vol 53 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Virginia Medeiros de Siqueira ◽  
Raphael Conti ◽  
Janete Magali de Araújo ◽  
Cristina Maria Souza-Motta
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Endophytic Fungi ◽  
Lippia Sidoides

scholarly journals Profiling Secondary Metabolites of Cocoa (Theobroma cacao L.) Endophytic Fungi Lasiodiplodia pseudotheobromae PAK-7 and Lasiodiplodia theobromae TN-R-3 and Their Antimicrobial Activities

2020 ◽  
pp. 47-56 ◽  
Author(s):  
M. Chaithra ◽  
S. Vanitha ◽  
A. Ramanathan ◽  
V. Jegadeeshwari ◽  
V. Rajesh ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Tamil Nadu ◽  
Statistical Design ◽  
Mevalonic Acid ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Metabolites ◽  
Chemical Compositions

Aims: To determine the chemical composition of secondary metabolite of cocoa endophytic fungi L. pseudotheobromae PAK-7, L. theobromae TN-R-3 and their anti-oomycete activities. Statistical Design: Multivariate analysis. Place and Duration of Study: Department of Plant Pathology, TNAU, Coimbatore, Tamil Nadu from April 2018 to December 2019. Methodology: Lipophilic extracellular secondary metabolites were extracted using ethyl acetate as a solvent and their chemical composition was detected by Gas Chromatography-Mass Spectrometry (GC-MS) and identified by NIST library and Pub Chem databases. Results: Metabolic profiling of cocoa endophytic fungi  L. pseudotheobromae PAK-7  and  L. theobromae TN-R-3  showed the presence of eleven peaks representing nine compounds. The most abundant compound observed were Acetic acid, 3-methyl-6-oxo-9oxabicyclo[3.3.1]non-2-yl ester, 2H-Pyran-2-one, tetrahydro-4-hydroxy-6-pentyl-, Melezitose, Ethyl à-d-glucopyranoside  collectively representing 58.01% area. In comparison to L. pseudotheobromae PAK-7 GC-MS analysis of L. theobromae TN-R-3 exhibited the presence of 29 peaks. The most abundant compounds were dl-Mevalonic acid lactone, Methyl 6-O-[1-methylpropyl]-á-d-galactopyranoside, 2H-Pyran-2-one, tetrahydro-4-hydroxy-6-pentyl-, Melezitose, Ethyl à-d-glucopyranoside, 1,6-Anhydro-á-d-talopyranose collectively representing 60.47% of the total area. Conclusion: Chemical compositions and anti-oomycete activities of crude secondary metabolites of L. pseudotheobromae PAK-7, L. theobromae TN-R-3 differed entirely depending on the property and abundance of bioactive metabolites.

Endophytic fungi from the root tubers of medicinal plant Stephania dielsiana and their antimicrobial activity

2020 ◽  
Vol 40 (5) ◽  
pp. 383-387
Author(s):  
Hongying Wu ◽  
Zhenling Yan ◽  
Yecheng Deng ◽  
Zhengjun Wu ◽  
Xianglin Xu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Endophytic Fungi

Antibacterial and antioxidant activities of endophytic fungi and nettle (Urtica dioica L.) leaves as their host

2021 ◽  
Vol 67 (3) ◽  
pp. 204-211
Author(s):  
Djouza Salmi ◽  
Catherine Riou ◽  
Mohammad Issawi ◽  
Yacine Titouche ◽  
Veronica Ambrosini ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Urtica Dioica ◽  
Biologically Active ◽  
Bioactive Metabolites ◽  
Wide Range ◽  
Thiol Content ◽  
Pharmaceutical Industries

Nettle (Urtica dioica L), as a plant rich in biologically active compounds, is one of the most important plants used in herbal medicine. Studies have shown that this plant has antioxidant, antiplatelet, hypoglycemic and hypocholesterolemia effects. In this study, we characterized three Alternaria endophytic fungi isolated from their host U. dioica. We hypothesized that these endophytic fungi can produce new bioactive metabolites, which may possess the bioactive property with potential application in the medical and pharmaceutical industries. The antibacterial activity was evaluated against reference and isolated strains, including Methicillin-Resistant Staphylococcus aureus. A wide range of antimicrobial activities similar to those measured in nettle leaves was detected especially for Alternaria sorghi. Furthermore, the highest antioxidant activity detected with DPPH free radical scavenging was measured for A. sorghi and nettle leaves ethyl acetate extracts. In addition, whereas catalase activity was similar in the three isolated fungi and nettle leaves, total thiol content and superoxide dismutase activity were significantly higher in leaves. A. sorghi showed the best activities compared to other isolated fungi. The characterization and further production of bioactive compounds produced by this endophyte should be investigated to fight bacteria and especially those that develop drug multi-resistance.

Antimicrobial activity of endophytic fungi associated with Orchidaceae in Brazil

2009 ◽  
Vol 55 (12) ◽  
pp. 1381-1391 ◽  
Author(s):  
Aline B.M. Vaz ◽  
Rubens C. Mota ◽  
Maria Rosa Q. Bomfim ◽  
Mariana L.A. Vieira ◽  
Carlos L. Zani ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Antimicrobial Activities ◽  
Tropical Ecosystem ◽  
Multivariate Statistical ◽  
Semideciduous Forest ◽  
Terrestrial Orchids ◽  
Alternaria Sp ◽  
Bacteria And Fungi ◽  
Bioactive Potential

The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of Orchidaceae collected in a Brazilian tropical ecosystem. In total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude extracts. Thirty-three percent of the isolates displayed antimicrobial activity against at least one target microorganism. The multivariate statistical analyses conducted indicate that the extracts of endophytic fungi isolated from leaves of terrestrial orchids in semideciduous forest were more active against Escherichia coli , whereas extracts of endophytic fungi from roots of rupicolous orchids collected in rock fields were more active against Candida krusei and Candida albicans . Among the fungi that were screened in the study, 22 isolates held their antimicrobial activities after replication and were therefore selected for assessment of the minimum inhibitory concentration (MIC), which ranged from 62.5 to 250 µg/mL and 7.8 to 250 µg/mL against bacteria and fungi, respectively. One isolate of Alternaria sp. and one isolate of Fusarium oxysporum presented the strongest antibacterial activity. Three Fusarium isolates, Epicoccum nigrum , and Sclerostagonospora opuntiae showed the greatest MIC values against the pathogenic yeasts. This study is the first survey investigating the bioactive potential of endophytic fungi associated with tropical Orchidaceae species present in Brazilian ecosystems.

Diversity and antimicrobial activity of endophytic fungi isolated from Nyctanthes arbor-tristis, a well-known medicinal plant of India

Mycoscience ◽  
2012 ◽  
Vol 53 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Surendra K. Gond ◽  
Ashish Mishra ◽  
Vijay K. Sharma ◽  
Satish K. Verma ◽  
Jitendra Kumar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Endophytic Fungi

scholarly journals Herbal mouthwash formulated with the leaf extract of Jatropha gossypiifolia Linn. (Euphorbiaceae) exhibited in vitro antimicrobial activity against selected oral pathogens

2021 ◽  
Vol 18 (3) ◽  
pp. 207-214
Author(s):  
Abel Olusola Idowu ◽  
Nwamaka Henrietta Igbokwe ◽  
Oreoluwa Ayomide Abiodun ◽  
Chijioke Ofomata
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Antimicrobial Activities ◽  
Oral Disease ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Root Bark ◽  
Oral Pathogens ◽  
Jatropha Gossypiifolia

Increase in the incidence of oral disease, resistance to antibiotics and adverse effect to synthetic medicines has made search for alternative safe, effective and cheaper treatment options imperative, preferably from plant sources. This study evaluated the antimicrobial profile of ethanol extracts of leaf and root bark of Jatropha gossypiifolia and mouthwash formulation containing the leaf extract. Extraction of plant parts was done by cold maceration with 70% ethanol. The antimicrobial activities of the extracts and the formulated mouthwash were evaluated againstStaphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Bacillus subtilis and Candida albicans using Agar well diffusion method. The minimum inhibitory concentration of the extracts on susceptible organisms was determined according to the Clinical Laboratory Standards Institute protocol. The physicochemical parameters of the formulated mouthwash were assessed using standard methods. J. gossypiifolia leaf and root bark extracts exhibited a concentration dependent antimicrobial activity and MIC ranging from 50 to 260 mg/ml against test organisms. The formulated herbal mouthwash showed effective antimicrobial activity and suitable cosmetics properties. This study indicates that the leaf extracts of J. gossypiifolia possess bioactive metabolites with strong antimicrobial activities and its herbal mouthwash formulation has potential in the treatment of various diseases caused by oral pathogens.

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