Odor fingerprinting of Listeria monocytogenes recognized by SPME–GC–MS and E-nose

2015 ◽  
Vol 61 (5) ◽  
pp. 367-372 ◽  
Author(s):  
Yong-xin Yu ◽  
Xiao-hong Sun ◽  
Yuan Liu ◽  
Ying-jie Pan ◽  
Yong Zhao
Keyword(s):  
Listeria Monocytogenes ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Growth Stages ◽  
Relative Peak Area ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Odor Compounds ◽  
Species Specific ◽  
Different Growth Stages

Microorganisms can produce species-specific microbial volatile organic compounds (MVOCs), or odor compounds, which can be characterized by odor fingerprinting. The objective of this study was to characterize the odor fingerprint of Listeria monocytogenes. Solid-phase microextraction – gas chromatography – mass spectrometry (SPME–GC–MS) and electronic nose (E-nose) were used to recognize the MVOCs of L. monocytogenes in pure culture medium. The main MVOCs of L. monocytogenes were identified by SPME–GC–MS analysis as alcohols, aldehydes, ketones, alkanes, and heterocyclics, among which the relative peak area of one compound, 3-hydroxy-2-butanone, increased along with the growth of L. monocytogenes. The odor fingerprint of L. monocytogenes at different growth stages could be clearly discriminated by E-nose. In addition, E-nose signals had a very good linear relationship with the concentration of this bacterium (R2 = 0.9937). Our study may help to establish the analysis of the odor fingerprint of microorganisms as a potential routine method in microbiology.

scholarly journals Detection and Identification of Microbial Volatile Organic Compounds of the Green Mold Disease

2020 ◽  
Vol 11 (2) ◽  
pp. 14-28
Author(s):  
Dalma Radványi ◽  
András Geösel ◽  
Zsuzsa Jókai ◽  
Péter Fodor ◽  
Attila Gere
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Identification System ◽  
Mushroom Species ◽  
Detection And Identification ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Green Mould

Button mushrooms are one of the most commonly cultivated mushroom species facing different risks e.g.: viral, bacterial and fungal diseases. One of the most common problems is caused by Trichoderma aggressivum, or ‘green mould' disease. The presence or absence of mushroom disease-related moulds can sufficiently be detected from the air by headspace solid-phase microextraction coupled gas chromatography-mass spectrometry (HS SPME GC-MS) via their emitted microbial volatile organic compounds (MVOCs). In the present study, HS SPME GC-MS was used to explore the volatile secondary metabolites released by T. aggressivum f. europaeum on different nutrient-rich and -poor media. The MVOC pattern of green mould was determined, then media-dependent and independent biomarkers were also identified during metabolomic experiments. The presented results provide the basics of a green mould identification system which helps producers reducing yield loss, new directions for researchers in mapping the metabolomic pathways of T. aggressivum and new tools for policy makers in mushroom quality control.

scholarly journals Tracking Bacterial Spoilage in Cosmetics by a New Bioanalytical Approach: API-SPME-GC-MS to Monitor MVOCs

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 38
Author(s):  
Maria Celeiro ◽  
Esther Varela ◽  
Rocio Rodriguez ◽  
Manuel Penedo ◽  
Marta Lores
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Practical Application ◽  
Biochemical Tests ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Original Approach

The main goal of this work was the use of the powerful solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) technique to unequivocally identify microbial volatile organic compounds (MVOCs) derived from the enzymatic activity produced during metabolic processes using analytical profile index (API) biochemical tests. Three bacteria were selected for this study: Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa. They were inoculated and incubated to both API components and real cosmetics, as well as to a mixture of them. Specific MVOCs were successfully identified as biomarkers for each one of the studied microorganisms: Indole and 2-nitrophenol as Escherichia coli markers, 2-undecanone and phenylethyl alcohol as Proteus mirabilis-specific markers, and 1-undecene and 2′-aminoacetophenone as Pseudomonas aeruginosa ones. In addition, a high number of MVOCs were identified as general markers of bacterial presence. The results revealed that the MVOCs’ formation is highly subtract dependent. Therefore, the ultimate and most challenging objective is to establish a relationship between the identified MVOCs and the original compound present in the substrate. This work establishes the design and development of this original approach, and its practical application to the control of microbial contamination in real cosmetic samples.

Fungal Infections of Fresh-Cut Fruit Can Be Detected by the Gas Chromatography–Mass Spectrometric Identification of Microbial Volatile Organic Compounds

2005 ◽  
Vol 68 (6) ◽  
pp. 1211-1216 ◽  
Author(s):  
STEVEN W. LLOYD ◽  
CASEY C. GRIMM ◽  
MAREN A. KLICH ◽  
SHANNON B. BELTZ
Keyword(s):  
Gas Chromatography ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Fungal Infections ◽  
Gas Chromatography Mass Spectrometry ◽  
Fungal Contamination ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Fresh Cut

There is a large and rapidly growing market for fresh-cut fruit. Microbial volatile organic compounds indicate the presence of fungal or bacterial contamination in fruit. In order to determine whether microbial volatile organic compounds can be used to detect contamination before fruit becomes unmarketable, pieces of cantaloupe, apple, pineapple, and orange were inoculated with a variety of fungal species, incubated at 25°C, then sealed in glass vials. The volatiles were extracted by headspace solid-phase microextraction and analyzed by gas chromatography–mass spectrometry. Forty-five compounds were identified that might serve as unique identifiers of fungal contamination. Fungal contamination can be detected as early as 24 h after inoculation.

scholarly journals Application of aluminosilicates for mitigation of ammonia and volatile organic compound emissions from poultry manure

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Sebastian Opaliński ◽  
Mariusz Korczyński ◽  
Marek Szołtysik ◽  
Zbigniew Dobrzański ◽  
Roman Kołacz
Keyword(s):  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Poultry Manure ◽  
Gas Chromatography Mass Spectrometry ◽  
Expanded Vermiculite ◽  
Volatile Organic ◽  
Livestock Buildings ◽  
Odor Compounds ◽  
Volatile Organic Compound Emissions ◽  
Livestock Building

AbstractOdor mitigation techniques are widely investigated due to the problem of odor nuisance generated by intensive livestock production. The goal of this research was to investigate the use of aluminosilicate sorbents as filter packs in the air scrubber ODOR1, which enables cleaning of air inside the livestock building. The following sorbents were examined: raw halloysite, roasted halloysite, activated halloysite, raw bentonite, roasted bentonite and expanded vermiculite. The experiment was conducted in chambers where poultry manure was placed, the time of air treatment was 24 hours. A manual SPME (solid-phase microextraction) holder with DVB/Carboxen/PDMS fiber was used for extraction of odor compounds, and analyses were carried out using gas chromatography-mass spectrometry. Ammonia concentrations were determined according to Polish standards (Nessler method) using a spectrophotometer. It was found that all examined aluminosilicates had the potential for removal of ammonia as well as 24 volatile compounds emitted from poultry manure. The highest efficiency was noted for activated halloysite (81%) and roasted bentonite (84%) in the case of ammonia and odors, respectively. Despite the limitations of the study, the results showed the effectiveness of the air scrubber packed with aluminosilicates for the reduction of volatile odorous compounds in the air of livestock buildings.

scholarly journals The Stool Volatile Metabolome of Pre-Term Babies

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3341
Author(s):  
Alessandra Frau ◽  
Lauren Lett ◽  
Rachael Slater ◽  
Gregory R. Young ◽  
Christopher J. Stewart ◽  
...  
Keyword(s):  
Early Life ◽  
Solid Phase ◽  
Mass Spectrometry Data ◽  
Gas Chromatography Mass Spectrometry ◽  
Mass Spectral ◽  
Branched Chain Fatty Acids ◽  
Volatile Organic ◽  
Stool Samples ◽  
Branched Chain ◽  
Mixed Modelling

The fecal metabolome in early life has seldom been studied. We investigated its evolution in pre-term babies during their first weeks of life. Multiple (n = 152) stool samples were studied from 51 babies, all <32 weeks gestation. Volatile organic compounds (VOCs) were analyzed by headspace solid phase microextraction gas chromatography mass spectrometry. Data were interpreted using Automated Mass Spectral Deconvolution System (AMDIS) with the National Institute of Standards and Technology (NIST) reference library. Statistical analysis was based on linear mixed modelling, the number of VOCs increased over time; a rise was mainly observed between day 5 and day 10. The shift at day 5 was associated with products of branched-chain fatty acids. Prior to this, the metabolome was dominated by aldehydes and acetic acid. Caesarean delivery showed a modest association with molecules of fungal origin. This study shows how the metabolome changes in early life in pre-term babies. The shift in the metabolome 5 days after delivery coincides with the establishment of enteral feeding and the transition from meconium to feces. Great diversity of metabolites was associated with being fed greater volumes of milk.

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