Cloning and function analysis of ClNAC9 from Chrysanthemum lavandulifolium

2018 ◽  
Vol 98 (6) ◽  
pp. 1265-1279
Author(s):  
Fengli Dong ◽  
He Huang ◽  
Jie Liu ◽  
Mi Zhang ◽  
Yunwei Zhou ◽  
...  
Keyword(s):  
Stress Responses ◽  
Expressed Sequence Tag ◽  
Transgenic Arabidopsis ◽  
Function Analysis ◽  
35S Promoter ◽  
Development Programs ◽  
Nac Transcription Factors ◽  
Chrysanthemum Lavandulifolium ◽  
Full Length Sequence ◽  
And Function

NAC transcription factors have been found to play an important role in several plant development programs and stress responses. In this study, a NAC gene, ClNAC9 (Chrysanthemum lavandulifolium NAC gene), was isolated from a cDNA library constructed according to the known expressed sequence tag sequence. The cDNA full-length sequence of ClNAC9 is comprised of 881 bp, encoding a putative protein of 217 amino acids. ClNAC9 has a conserved NAC domain in the N-terminus — the NAM domain. ClNAC9 is highly similar to other NACs, especially SENU5 subgroup members. Transgenic Arabidopsis overexpressing ClNAC9 controlled by the CaMV-35S promoter was generated and subjected to saline, alkaline, and drought stresses for morphological and physiological assays. Morphological analyses showed that transgenic plants had enhanced tolerance to saline, alkaline, and drought stresses, as indicated by improved physiological traits, including higher superoxide dismutase and peroxidase activities, and reduced malondialdehyde accumulation. Moreover, overexpression of ClNAC9 enhanced up-regulation of RD29A, RD26, MYB2, and MYB96 expression, and reduced HAB1 expression under saline, alkaline, and drought treatments. Taken together, our results demonstrate that ClNAC9 is likely related to saline, alkaline, and drought resistances and overexpression of ClNAC9 increases the saline, alkaline, and drought resistance of transgenic Arabidopsis.

scholarly journals Expression Pattern and Function Analysis of AtPPRT1, a Novel Negative Regulator in ABA and Drought Stress Responses in Arabidopsis

2019 ◽  
Vol 20 (2) ◽  
pp. 394 ◽  
Author(s):  
Linsen Pei ◽  
Lu Peng ◽  
Xia Wan ◽  
Jie Xiong ◽  
Zhibin Liu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Responses ◽  
Function Analysis ◽  
Lateral Roots ◽  
Negative Regulator ◽  
Abiotic Stress Response ◽  
Root Tips ◽  
E3 Ligases ◽  
Ubiquitin E3 Ligase ◽  
And Function

Abscisic acid (ABA) plays a fundamental role in plant growth and development, as well as in the responses to abiotic stresses. Previous studies have revealed that many components in ABA and drought stress signaling pathways are ubiquitinated by E3 ligases. In this study, AtPPRT1, a putative C3HC4 zinc-finger ubiquitin E3 ligase, was explored for its role in abiotic stress response in Arabidopsis thaliana. The expression of AtPPRT1 was induced by ABA. In addition, the β-glucuronidase (GUS) gene driven by the AtPPRT1 promoter was more active in the root hair zone and root tips of primary and major lateral roots of young seedlings in the presence of ABA. The assays for seed germination, stomatal aperture, root length, and water deficit demonstrated that the AtPPRT1-overexpressing Arabidopsis was insensitive to ABA and sensitive to drought stress compared with wild-type (WT) plants. The analysis by quantitative real-time PCR (qRT-PCR) revealed that the expression of three stress-inducible genes (AtRAB18, AtERD10, and AtKIN1) were upregulated in the atpprt1 mutant and downregulated in AtPPRT1-overexpressing plants, while two ABA hydrolysis genes (AtCYP707A1 and AtCYP707A3) were downregulated in the atpprt1 mutant and upregulated in AtPPRT1-overexpressing plants in the presence of ABA. AtPPRT1 was localized in the mitochondria. Our findings indicate that AtPPRT1 plays a negative role in ABA and drought stress responses.

Structure and function analysis of a CC-NBS-LRR protein AT1G12290

2021 ◽  
Vol 534 ◽  
pp. 206-211
Author(s):  
Jianzhong Huang ◽  
Xiaoqiu Wu ◽  
Kaiting Sun ◽  
Zhiyong Gao
Keyword(s):  
Function Analysis ◽  
Structure And Function ◽  
And Function ◽  
Lrr Protein

Characterization of diacylglycerol acyltransferase 2 from Idesia polycarpa and function analysis

2021 ◽  
Vol 234 ◽  
pp. 105023
Author(s):  
Ruishen Fan ◽  
Gui Cai ◽  
Xuanyuan Zhou ◽  
Yuxin Qiao ◽  
Jiabao Wang ◽  
...  
Keyword(s):  
Function Analysis ◽  
Diacylglycerol Acyltransferase ◽  
And Function

scholarly journals OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Xiang Zhang ◽  
Yan Long ◽  
Jingjing Huang ◽  
Jixing Xia
Keyword(s):  
Transcription Factors ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Crop Yields ◽  
Plant Stress ◽  
Root Cells ◽  
Nac Transcription Factors ◽  
Rice Plants ◽  
Plant Stress Responses

Abstract Background Salt stress threatens crop yields all over the world. Many NAC transcription factors have been reported to be involved in different abiotic stress responses, but it remains unclear how loss of these transcription factors alters the transcriptomes of plants. Previous reports have demonstrated that overexpression of OsNAC45 enhances salt and drought tolerance in rice, and that OsNAC45 may regulate the expression of two specific genes, OsPM1 and OsLEA3–1. Results Here, we found that ABA repressed, and NaCl promoted, the expression of OsNAC45 in roots. Immunostaining showed that OsNAC45 was localized in all root cells and was mainly expressed in the stele. Loss of OsNAC45 decreased the sensitivity of rice plants to ABA and over-expressing this gene had the opposite effect, which demonstrated that OsNAC45 played an important role during ABA signal responses. Knockout of OsNAC45 also resulted in more ROS accumulation in roots and increased sensitivity of rice to salt stress. Transcriptome sequencing assay found that thousands of genes were differently expressed in OsNAC45-knockout plants. Most of the down-regulated genes participated in plant stress responses. Quantitative real time RT-PCR suggested that seven genes may be regulated by OsNAC45 including OsCYP89G1, OsDREB1F, OsEREBP2, OsERF104, OsPM1, OsSAMDC2, and OsSIK1. Conclusions These results indicate that OsNAC45 plays vital roles in ABA signal responses and salt tolerance in rice. Further characterization of this gene may help us understand ABA signal pathway and breed rice plants that are more tolerant to salt stress.

scholarly journals Molecular characterization and function analysis of the rice OsDUF617 family

2021 ◽  
Vol 35 (1) ◽  
pp. 862-872
Author(s):  
Chunliu Li ◽  
Dejia Hou ◽  
Lin Zhang ◽  
Xiaohong Li ◽  
Jiangbo Fan ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Function Analysis ◽  
And Function

scholarly journals Genome-wide identification and analysis of class III peroxidases in Betula pendula

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Kewei Cai ◽  
Huixin Liu ◽  
Song Chen ◽  
Yi Liu ◽  
Xiyang Zhao ◽  
...  
Keyword(s):  
Stress Responses ◽  
Betula Pendula ◽  
Expression Patterns ◽  
Class Iii ◽  
Physiological Processes ◽  
Plant Life ◽  
Genome Wide ◽  
Plant Life Cycle ◽  
Class Iii Peroxidases ◽  
And Function

Abstract Background Class III peroxidases (POD) proteins are widely present in the plant kingdom that are involved in a broad range of physiological processes including stress responses and lignin polymerization throughout the plant life cycle. At present, POD genes have been studied in Arabidopsis, rice, poplar, maize and Chinese pear, but there are no reports on the identification and function of POD gene family in Betula pendula. Results We identified 90 nonredundant POD genes in Betula pendula. (designated BpPODs). According to phylogenetic relationships, these POD genes were classified into 12 groups. The BpPODs are distributed in different numbers on the 14 chromosomes, and some BpPODs were located sequentially in tandem on chromosomes. In addition, we analyzed the conserved domains of BpPOD proteins and found that they contain highly conserved motifs. We also investigated their expression patterns in different tissues, the results showed that some BpPODs might play an important role in xylem, leaf, root and flower. Furthermore, under low temperature conditions, some BpPODs showed different expression patterns at different times. Conclusions The research on the structure and function of the POD genes in Betula pendula plays a very important role in understanding the growth and development process and the molecular mechanism of stress resistance. These results lay the theoretical foundation for the genetic improvement of Betula pendula.

Cytochrome P450 mono-oxygenases in conifer genomes: discovery of members of the terpenoid oxygenase superfamily in spruce and pine

2006 ◽  
Vol 34 (6) ◽  
pp. 1209-1214 ◽  
Author(s):  
B. Hamberger ◽  
J. Bohlmann
Keyword(s):  
Cytochrome P450 ◽  
Loblolly Pine ◽  
Large Scale ◽  
Expressed Sequence Tag ◽  
Resin Acid ◽  
Functional Characterization ◽  
Structural Diversity ◽  
Resin Acids ◽  
Full Length Sequence ◽  
Diterpene Resin Acids

Diterpene resin acids, together with monoterpenes and sesquiterpenes, are the most prominent defence chemicals in conifers. These compounds belong to the large group of structurally diverse terpenoids formed by enzymes known as terpenoid synthases. CYPs (cytochrome P450-dependent mono-oxygenases) can further increase the structural diversity of these terpenoids. While most terpenoids are characterized as specialized or secondary metabolites, some terpenoids, such as the phytohormones GA (gibberellic acid), BRs (brassinosteroids) and ABA (abscisic acid), have essential functions in plant growth and development. To date, very few CYP genes involved in conifer terpenoid metabolism have been functionally characterized and were limited to two systems, yew (Taxus) and loblolly pine (Pinus taeda). The characterized yew CYP genes are involved in taxol diterpene biosynthesis, while the only characterized pine terpenoid CYP gene is part of DRA (diterpene resin acid) biosynthesis. These CYPs from yew and pine are members of two apparently conifer-specific CYP families within the larger CYP85 clan, one of four plant CYP multifamily clans. Other CYP families within the CYP85 clan were characterized from a variety of angiosperms with functions in terpenoid phytohormone metabolism of GA, BR, and ABA. The recent development of EST (expressed sequence tag) and FLcDNA (where FL is full-length) sequence databases and cDNA collections for species of two conifers, spruce (Picea) and pine, allows for the discovery of new terpenoid CYPs in gymnosperms by means of large-scale sequence mining, phylogenetic analysis and functional characterization. Here, we present a snapshot of conifer CYP data mining, discovery of new conifer CYPs in all but one family within the CYP85 clan, and suggestions for their functional characterization. This paper will focus on the discovery of conifer CYPs associated with diterpene metabolism and CYP with possible functions in the formation of GA, BR, and ABA in conifers.

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