EFFECTS OF POTASSIUM ACID PHOSPHATE, CANE SUGAR, ETHYL MERCURIC BROMIDE, AND INDOLYLACETIC ACID IN A TALC CARRIER ON THE ROOTING OF STEM CUTTINGS

1941 ◽  
Vol 19c (4) ◽  
pp. 99-105
Author(s):  
N. H. Grace
Keyword(s):  
Acid Treatment ◽  
Cane Sugar ◽  
Stem Cuttings ◽  
Organic Mercury ◽  
Acid Phosphate ◽  
Rooted Cuttings ◽  
Indolylacetic Acid ◽  
Beneficial Effects ◽  
Plant Stem

Greenwood cuttings of Deutzia Lemoinei, Symphoricarpus albus, and Weigela rosea and dormant cuttings of Lonicera tatarica were treated with a series of 32 talc dusts containing potassium acid phosphate at concentrations of 0, 0.1, 1.0, and 10%, in combination with 0 and 10% cane sugar, 0 and 50 p.p.m. ethyl mercuric bromide, and 0 and 1000 p.p.m. indolylacetic acid. The lower concentrations of phosphate tended to increase rooting and reduce mortality of two of the species of greenwood cuttings whereas the 10% concentration was ineffective or injurious. However, this concentration was favourable to the rooting of dormant cuttings. Indolylacetic acid treatment increased the number of rooted cuttings and the number and length of roots. Beneficial effects were indicated for organic mercury and cane sugar treatments. However, these were attributed largely to the combinations with phosphate and indolylacetic acid. The results indicate that the effectiveness of dusts containing indolylacetic acid in the treatment of plant stem cuttings may be increased by the addition of nutrient and disinfectant chemicals.

VEGETATIVE PROPAGATION OF CONIFERS: IX. EFFECTS OF CHEMICAL TREATMENTS AND A WAX SPRAY ON THE OUTDOOR PROPAGATION OF SPRUCE CUTTINGS

1941 ◽  
Vol 19c (7) ◽  
pp. 257-266
Author(s):  
N. H. Grace ◽  
J. L. Farrar
Keyword(s):  
Acid Treatment ◽  
Cane Sugar ◽  
Organic Mercury ◽  
Acid Phosphate ◽  
Indolylacetic Acid ◽  
Beneficial Effects ◽  
Chemical Treatments ◽  
Eastern White Cedar ◽  
Wax Coating ◽  
Injurious Effects

Norway spruce cuttings were collected at intervals throughout the year, subjected to treatment with talc dusts containing from 5 up to 10,000 p.p.m. of indolylacetic acid, and propagated in outdoor frames. In some experiments, indolylacetic acid treatments were included in a series of dusts involving cane sugar, potassium acid phosphate, and organic mercurial disinfectants. Effects of wax coating of cuttings of Norway and white spruce and eastern white cedar were also considered.Indolylacetic acid treatment failed to have any general beneficial effects on rooting; concentrations of 8000 and 10,000 p.p.m. were usually injurious. However, treatment of apparently dormant cuttings, taken shortly before emergence of new growth, with 1000 p.p.m. gave 25% rooting, as compared to 8% for the controls, and tended to increase the length of root. Treatments with organic mercury, cane sugar, and potassium acid phosphate increased survival of new growth, and, in combination with indolylacetic acid, increased survival and root lengths.Talc treatment increased rooting to as much as 70% for cuttings planted in sand as compared to 25% for the controls, but had no effect on plantings in a sand–peat mixture. Talc effects were the most marked on cuttings taken before emergence of new growth and when planting was delayed for 24 hr. after treatment.Wax application had no effect on dormant spruce cuttings but was markedly injurious to summer collections. Injurious effects were reduced when wax was used in conjunction with indolylacetic acid treatment.

VEGETATIVE PROPAGATION OF CONIFERS: VI. HORMONE SOLUTION AND DUST TREATMENTS OF SPRUCE CUTTINGS PROPAGATED IN GREENHOUSE AND OUTSIDE FRAMES

1940 ◽  
Vol 18c (8) ◽  
pp. 401-414 ◽  
Author(s):  
N. H. Grace ◽  
J. L. Farrar
Keyword(s):  
Beneficial Effect ◽  
Norway Spruce ◽  
Vegetative Propagation ◽  
Acid Treatment ◽  
Black Spruce ◽  
Cane Sugar ◽  
The Other ◽  
Acid Solutions ◽  
Organic Mercury ◽  
Indolylacetic Acid

Four monthly collections of dormant Norway spruce cuttings, January to April, were treated with indolylbutyric acid solutions and propagated in sand in a greenhouse. February and April collections gave better rooting than those of the other two months, while there was appreciably greater mortality of the March and April collections. The results with short cuttings were uniformly superior to those obtained with long cuttings. Other cuttings of the April collection were propagated outside. The short cuttings responded more favourably to outside conditions, while the long cuttings gave equally poor results inside and outside. Indolylbutyric acid treatment had no beneficial effect and was followed by reduced rooting and increased mortality at concentrations from 20 to 60 p.p.m.Results of a late March collection of Norway, white, and black spruce cuttings treated with talc dusts containing indolylacetic acid, cane sugar, and organic mercury, indicated that Norway spruce rooted more readily than the two other species. Treatment failed to have a beneficial effect, although injury from indolylacetic acid was somewhat reduced by its combination with organic mercury.The results of these and the other experiments reported indicate that short Norway spruce cuttings over the period from January to April root to the extent of about 50%. A May collection, an early June collection with new growth on the cuttings, and a late June collection in which the cuttings were made from new growth only, gave inappreciable rooting. Similar new-growth cuttings did, however, give some rooting when propagated in sand watered with nutrient salts.

VEGETATIVE PROPAGATION OF CONIFERS: VII. OUTDOOR PROPAGATION OF A NOVEMBER COLLECTION OF NORWAY SPRUCE CUTTINGS TREATED WITH PHYTOHORMONES, CANE SUGAR, AND AN ORGANIC MERCURIAL DISINFECTANT

1940 ◽  
Vol 18c (11) ◽  
pp. 566-577
Author(s):  
N. H. Grace ◽  
J. L. Farrar ◽  
J. W. Hopkins
Keyword(s):  
Norway Spruce ◽  
Vegetative Propagation ◽  
Cane Sugar ◽  
Organic Mercury ◽  
Rooted Cuttings ◽  
Indolylacetic Acid ◽  
Propagation Medium ◽  
Naphthylacetic Acid ◽  
Phytohormone Treatment

Dormant Norway spruce cuttings were collected in mid-November and treated with talc dusts containing two separate phytohormone chemicals, indolylacetic and naphthylacetic acids, in three concentrations, 0, 1000, and 5000 p.p.m., alone, and in combination with cane sugar and an organic mercurial disinfectant. Treated cuttings were planted immediately in outside frames in sand and a mixture of sand and peat in equal proportions. They were removed for examination 10 months later. Phytohormone treatment, except with the 5000 p.p.m. concentration of naphthylacetic acid, which was injurious, increased the number of cuttings rooted, the number and length of roots, the number of surviving cuttings, and the number of rooted cuttings with new growth. There were also marked effects on the initiation and development of new growth. The admixture of peat in the propagation medium improved rooting and development of new growth, particularly in certain of the phytohormone treatments. Only about 50% of the controls rooted in both sand and sand-peat, while the 5000 p.p.m. concentration of indolylacetic acid effected 68% rooting in sand and 82% in sand-peat.Both cane sugar and organic mercury significantly affected a number of the responses. However, the effects were comparatively small and depended, for the most part, on interactions with phytohormone treatments and media. Organic mercury increased the number of cuttings rooted by about 6%.

RESPONSES OF DORMANT CUTTINGS OF LONICERA TARTARICA TO SOLUTIONS OF CANE SUGAR AND INDOLYLACETIC ACID

1939 ◽  
Vol 17c (10) ◽  
pp. 334-338
Author(s):  
N. H. Grace
Keyword(s):  
Acid Treatment ◽  
Solution Treatment ◽  
Cane Sugar ◽  
Root Weight ◽  
Rooted Cutting ◽  
Indolylacetic Acid ◽  
Parallel Experiment ◽  
Factorial Series ◽  
Effect Of Treatment ◽  
Fresh Root

Cuttings of dormant Lonicera tartarica, collected in March, were treated with a factorial series of indolylacetic acid and cane sugar solutions. Indolylacetic acid was used at concentrations of 0, 10, 50, and 100 p.p.m., while cane sugar was present at 0, 1, 5, and 10%. Indolylacetic acid treatment greatly increased the percentage of cuttings rooted, the number and length of roots per rooted cutting, the fresh root weight and the green weight of leaf produced. Cane sugar treatment alone or in combination with indolylacetic acid failed to show any significant effects, suggesting that dormant cuttings of this plant have an adequate reserve of carbohydrate material.Apart from a somewhat greater effect of treatment on the percentage of rooting, the results are in essential agreement with those previously secured from dormant October cuttings. In comparison with a parallel experiment on the dusting of March cuttings propagated in the same frame, solution treatment had the greater effect on all the responses considered except green weight of leaf produced, which was greater following dusting.

VEGETATIVE PROPAGATION OF CONIFERS: IV. EFFECTS OF CANE SUGAR, ETHYL MERCURIC PHOSPHATE, AND INDOLYLACETIC ACID IN TALC DUST ON THE ROOTING OF NORWAY SPRUCE

1940 ◽  
Vol 18c (1) ◽  
pp. 13-17
Author(s):  
N. H. Grace
Keyword(s):  
Norway Spruce ◽  
Vegetative Propagation ◽  
Root Length ◽  
Cane Sugar ◽  
Organic Mercury ◽  
Rooted Cutting ◽  
Indolylacetic Acid ◽  
Naphthylacetic Acid

Norway spruce cuttings collected in November from the upper part of the tree were treated with a series of talc dusts containing indolylacetic and naphthylacetic acids, each at 0, 1000, and 5000 p.p.m., combined with cane sugar at 0 and 10%, and ethyl mercuric phosphate at 0 and 50 p.p.m., and propagated in a greenhouse. Indolylacetic acid at 1000 p.p.m. increased rooting by about 10% to 42.5%, three months after planting, but at 5000 p.p.m. reduced it significantly. Naphthylacetic acid reduced rooting at both concentrations. Indolylacetic acid increased the length of root per rooted cutting. Sugar in combination with indolylacetic acid inhibited the increase in root length caused by the hormone alone; organic mercury alone or in combination with indolylacetic acid reduced root length, but sugar and mercury in combination exerted no greater inhibition than either alone.Mean root length was affected only by indolylacetic acid, which increased it.

RESPONSES OF PLANT STEM CUTTINGS TREATED WITH CHEMICALS IN A CARRIER DUST

1945 ◽  
Vol 23c (4) ◽  
pp. 115-126
Author(s):  
N. H. Grace
Keyword(s):  
Nicotinic Acid ◽  
Root Length ◽  
Physiological Activity ◽  
Metallic Silver ◽  
Stem Cuttings ◽  
Organic Mercury ◽  
Nutrient Salt ◽  
Factorial Series ◽  
The Mean ◽  
Plant Stem

Plant stem cuttings were treated in a factorial series with talc dusts containing naphthylbutyric acid, nutrient salts, an organic mercurial disinfectant, finely divided silver metal, thiamin, and nicotinic acid. Naphthylbutyric acid treatment increased the number of roots for cuttings of Physocarpus and Iresine and reduced the mean root length of Lonicera. Nutrient salt treatment increased the number of roots on Iresine cuttings and the mean root length of Lonicera. Differential effects were found for treatments with naphthylbutyric acid and each of organic mercury, metallic silver, and nutrient salts. Organic mercury at 50 p.p.m. and metallic silver at 5000 p.p.m. were similar in their effects. Thiamin and nicotinic acid had no significant effect on the rooting responses.Naphthylacetic acid was prepared in talc at concentrations of 4000, 2000, 1000, and 500 p.p.m. and indolylbutyric, naphthylbutyric acids, and potassium naphthylhexoate at molecularly equivalent concentrations. When applied in a talc carrier dust to cuttings of seven species naphthylacetic and indolylbutyric acids were substantially similar in their effects while naphthylbutyric acid and potassium naphthylhexoate showed a lower order of physiological activity. Effects of chemicals judged by the rooting responses of cuttings tended to be similar over the concentration range. On the average, the 4000 p.p.m. dosage was injurious, while the two lowest dosages failed to differ in effects between themselves.

EFFECTS OF CANE SUGAR, ETHYL MERCURIC PHOSPHATE, AND INDOLYLACETIC ACID IN TALC ON THE ROOTING OF CUTTINGS

1939 ◽  
Vol 17c (10) ◽  
pp. 321-333
Author(s):  
N. H. Grace
Keyword(s):  
Dry Weight ◽  
Cane Sugar ◽  
Root Mass ◽  
Organic Mercury ◽  
Rooted Cutting ◽  
Indolylacetic Acid ◽  
Factorial Series ◽  
Rooting Of Cuttings ◽  
Physocarpus Opulifolius ◽  
Fresh Root

Cuttings of two herbaceous and two dormant woody plants were treated with a factorial series of talc dusts containing cane sugar, ethyl mercuric phosphate and indolylacetic acid. The effect of the dusts on cuttings of Coleus Blumei and Iresine Lindeni was determined by the number of roots per rooted cutting, the length of root mass and dry weight of roots. Each of the three factors gave at least one significant effect with both plants. Iresine Lindeni cuttings showed two significant interactions, one between organic mercury and indolylacetic acid on the number of roots per rooted cutting, the other between sugar and indolylacetic acid on the length of root mass. Dormant Lonicera tartarica cuttings showed significant effects from indolylacetic acid on the number of cuttings rooted, the number and length of roots per rooted cutting, the mean root length, and fresh root weights. Green leaf weights of this plant were significantly affected by sugar, and the sugar × organic mercury, and sugar × organic mercury × indolylacetic acid interactions. Fresh root weights also gave a significant triple interaction. The number of Physocarpus opulifolius cuttings rooted was significantly increased by organic mercury as were the dry root weights. Root weights also were affected by sugar treatment. This plant failed to make any significant response to indolylacetic acid treatment.The results indicate that cane sugar and ethyl mercuric phosphate, as well as indolylacetic acid, affect some of the rooting responses of plant cuttings. It is suggested that the dust method of treating cuttings may be used to supply factors, other than the recognized growth stimulating chemicals, that are advantageous to successful vegetative propagation of plants.

scholarly journals Cutting Type and IBA Treatment Duration Affect Teucrium fruticans Adventitious Root Quality

2014 ◽  
Vol 42 (2) ◽  
pp. 478-481 ◽  
Author(s):  
Leo SABATINO ◽  
Fabio D’ANNA ◽  
Giovanni IAPICHINO
Keyword(s):  
Acid Solution ◽  
Butyric Acid ◽  
Root Development ◽  
Root Length ◽  
Treatment Duration ◽  
Acid Treatment ◽  
Stem Cuttings ◽  
Root Number ◽  
Rooted Cuttings ◽  
Root Quality

Root development of stem cuttings of Silver Germander (Teucrium fruticans) was investigated in relation to cutting type and indole-3-butyric acid (IBA) treatment. Terminal cuttings of a clone grown in Sicily were trimmed to three types: terminal cuttings with apex (TWA), terminal cuttings without apex (TWOA) or sub-terminal cuttings (ST). To verify the cutting response to exogenous auxin, cuttings were dipped to a 2.0 cm depth in a 0.5% indole-3-butyric acid solution for 0, 5 or 7 minutes. Overall percent survival was 97 to 98%. Rooting percent, root number and root length were affected by cutting type and indole-3-butyric acid treatment. In general, TWA cuttings demonstrated a higher capacity to form roots than cuttings without apex (TWOA and ST cuttings). In absence of indole-3-butyric acid treatment, TWA and ST cuttings gave higher rooting percentages than TWOA cuttings. Exposing cuttings to indole-3-butyric acid improved percent rooting, number of roots per cutting and root length. The best results in terms of rooting percentage and root number per cuttings were obtained with TWA cuttings in combination with 7 min indole-3-butyric acid basal dip. However, cuttings taken farther down the stem, such as sub-terminal cuttings gave satisfactory rooting performance as well. We suggest that the use of all cutting types tested associated to indole-3-butyric acid basal dip for 5 or 7 min may be beneficial to propagators wishing to produce T. fruticans rooted cuttings with well-developed root system.

EFFECTS OF TALC DUSTS CONTAINING PHYTOHORMONE, NUTRIENT SALTS, AND AN ORGANIC MERCURIAL DISINFECTANT ON THE ROOTING OF HERBACEOUS CUTTINGS

1941 ◽  
Vol 19c (5) ◽  
pp. 177-182 ◽  
Author(s):  
N. H. Grace
Keyword(s):  
Acid Treatment ◽  
Root Weight ◽  
Organic Mercury ◽  
Rooted Cutting ◽  
Beneficial Effects ◽  
Coleus Blumei ◽  
Fresh Root

Cuttings of Coleus Blumei, varieties of Chrysanthemum and species and varieties of Iresine were treated with a series of talc dusts containing naphthylbutyric acid, nutrient salts, and ethyl mercuric bromide and then were propagated in sand in the greenhouse. Naphthylbutyric acid treatment increased the number of roots per rooted cutting, and its combination with the mixture of nutrient salts increased fresh root weight of Coleus cuttings. Organic mercury treatment increased, by about 5%, the number of Chrysanthemum cuttings that rooted, and increased the number of roots on Iresine cuttings. Beneficial effects from talc treatment alone were a feature of the results. Differential reactions to both talc and organic mercury treatments were shown by closely related varieties.

scholarly journals Submist is Effective for Propagation of Korean Lilac and Inkberry by Stem Cuttings

2018 ◽  
Vol 28 (3) ◽  
pp. 378-381
Author(s):  
Bryan J. Peterson ◽  
Stephanie E. Burnett ◽  
Olivia Sanchez
Keyword(s):  
Butyric Acid ◽  
Woody Plants ◽  
Root Biomass ◽  
Potassium Salt ◽  
Foliar Nutrients ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Soilless Substrate

Although overhead mist revolutionized the propagation industry, it does suffer from potential drawbacks that include the application of large volumes of water, potentially unsanitary conditions, irregular misting coverage, and leaching of foliar nutrients. We explored the feasibility of submist as an alternative as it might avoid these problems by applying water exclusively from below the cutting, which is inserted basally into an enclosed rooting chamber. We propagated cuttings of korean lilac (Syringa pubescens ssp. patula) and inkberry (Ilex glabra) using both overhead mist and submist to compare effectiveness of the systems. Cuttings of korean lilac were wounded and dipped basally into 8000 mg·L−1 of the potassium salt of indole-3-butyric acid (K-IBA), and those in the overhead mist systems were inserted into coarse perlite. Cuttings of inkberry were wounded and treated with 5000 mg·L−1 K-IBA, and those in the overhead mist systems were inserted into 50:50 peat:perlite (by vol). Cuttings of korean lilac in the submist systems produced more than twice as many roots as cuttings in the overhead mist systems, with roots more than 2.6 times the length. Similarly, cuttings of inkberry in the submist systems produced more than three times the root counts and root lengths as cuttings in the overhead mist systems. For korean lilac, root dry weights averaged 58 mg for cuttings in the submist system, compared with only 18 mg among cuttings receiving overhead mist. Likewise, root dry weights averaged 70 and 7 mg for cuttings of inkberry propagated by submist and overhead mist, respectively. Rooted cuttings of korean lilac transplanted well into a soilless substrate, where they more than tripled their root biomass to 218 mg (vs. 59 mg for cuttings transplanted from overhead mist). We did not evaluate transplant performance of inkberry. Our results show that submist systems might merit consideration for the propagation of woody plants by leafy stem cuttings.

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